Click here to switch to the map view.

The map label for this gene is merA [H]

Identifier: 187928870

GI number: 187928870

Start: 1882384

End: 1884069

Strand: Direct

Name: merA [H]

Synonym: Rpic_1787

Alternate gene names: 187928870

Gene position: 1882384-1884069 (Clockwise)

Preceding gene: 187928869

Following gene: 187928871

Centisome position: 47.75

GC content: 68.86

Gene sequence:

>1686_bases
ATGACCCATCTGAAGATCACTGGAATGACCTGCGATTCGTGTGCCGTCCACGTCAAGGATGCCTTGGAGAAGGTGCCGGG
CGTGCGGTCGGCGGTCGTATCCTATCCGAAGGGGACGGCGCAAATCGCTACCGACCCCGGCACTTCGACGGATGCGCTGA
CTGCGGCCGTGGCCGGACTCGGCTACCGGGTCATGCTCGGTGAGGCCCCGCCAGTGGAAAAACGCGGCAACCTGCTCGAC
AAGGCGCTGGGCTGGCTTGCCAGCGGTGAGAAATCCGCAGGCGAAGGCAGCGCGCTACGCATCGCCGTCATCGGCAGCGG
CGGCGCCGCGATGGCGGCGGCCCTCAAGGCCGTCGAGCAAGGCGCGCAGGTCACCCTGATCGAGCGCGGCACCATCGGTG
GCACCTGCGTCAACGTCGGCTGCGTGCCGTCCAAGATCATGATCCGCGCGGCCCATATCGCCCACCTGCGCCGCGAAAGC
CCGTTCGACGGCGGCATCCAGGCCACACCGCCGGCCATCGTGCGCGAACGCCTGCTGGCCCAGCAGCAGGGGCGCGTCGA
TGAGCTGCGCCACGCTAAGTACGAGGGCATCCTGGCCAGCACCCCGGCCATCACGGTGCTGCGCGGCGAGGCCCGCTTCC
AGAACGGGCATGCCCTGAGCGTGCGCCTGGCCGAGGGCGGTGAGCGCGAGGTGGCATTTGACCGCTGCCTGATCGCCACG
GGCGCCAGCGCGGCCGTGCCGCCGATCCCCGGCCTGAAGGACACCCCATACTGGACCTCGAACGAGGCGCTGGCCAGCGA
CACCCTGCCCAAGCGGCTGGCCGTGATCGGCTCGTCGGTGGTGGCCGTGGAGCTGGCGCAGGCATTCGCCCGGCTGGGCA
GCCAGGTCACGGTCCTGGCGCGCAGCACGCTGTTCTTCCGTGAGGATCCGGCCATCGGCGAGGCCGTGACGGCCGCGTTC
CGCGCCGAGGGCATCACGGTGCTGGACCACACGCAGGCCAGCCAGGTGGCCTACGCGAACGGTGAATTCGTGCTCACCAC
CGGGCACGGTGAAATCCGCGCCGACCAGTTGCTGGTCGCCACCGGCCGCGCGCCGAACACGCGTGAGCTGGCGCTGGAGG
CGGCGGGGGTGAGGGACAACGCGCAAGGGGCCATCGTCATCGACAAGGCGATGCGCACCAGCGCACAGAACATCTACGCC
GCCGGCGATTGCACCGACCAGCCGCAATTCGTCTATGTGGCCGCAGCGGCCGGCACCCGCGCGGCGATCAACATGACCGG
AGGCGATGCCGCGCTCGATCTGAGCGCCGTGCCGGCGGTGGTGTTCACCGACCCGCAGGTCGCCACCGTTGGCTACAGCG
AAGCGGAAGCGCACCACGATGGCATCGAGACCGATAGCCGGCTGCTCACGCTGGACAACGTGCCGCGGGCGCTCGCCAAC
TTCGACACGCGCGGCTTCATCAAGCTGGTCGCTGATGCTGGCAGTGGGCGCTTGATCGGCGTGCAGGCGGTGGCGCCGGA
AGCGGGTGAACTGATCCAGACGGCGGCCCTGGCAATTCGCAACCGCATGACGGTGCAGGAACTGGCCGACCAGTTGTTCC
CGTACCTGACCATGGTCGAGGGACTCAAGCTTGCCGCGCAGACCTTCTCCAAGGACGTGAAGCAGCTGTCCTGCTGCGCT
GGGTGA

Upstream 100 bases:

>100_bases
ACCGGCGAAGCGCTGCAGCGCACCGGACACCTGTGAACTCCCGGCCAAACGCGGCTGACGCTTTAGCCCTGTATCACTGT
ATCAAAGAAGGAACTGTGGC

Downstream 100 bases:

>100_bases
GGCAAAGGAGGTGTTCGATGAATGCCTACACAGTGTCGCGTCTGGCCCACGATGCCAGCGTGAGCGTCAACGTGGTGCGT
GACTATGCGCTGCGCGGCCT

Product: putative mercuric reductase

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 561; Mature: 560

Protein sequence:

>561_residues
MTHLKITGMTCDSCAVHVKDALEKVPGVRSAVVSYPKGTAQIATDPGTSTDALTAAVAGLGYRVMLGEAPPVEKRGNLLD
KALGWLASGEKSAGEGSALRIAVIGSGGAAMAAALKAVEQGAQVTLIERGTIGGTCVNVGCVPSKIMIRAAHIAHLRRES
PFDGGIQATPPAIVRERLLAQQQGRVDELRHAKYEGILASTPAITVLRGEARFQNGHALSVRLAEGGEREVAFDRCLIAT
GASAAVPPIPGLKDTPYWTSNEALASDTLPKRLAVIGSSVVAVELAQAFARLGSQVTVLARSTLFFREDPAIGEAVTAAF
RAEGITVLDHTQASQVAYANGEFVLTTGHGEIRADQLLVATGRAPNTRELALEAAGVRDNAQGAIVIDKAMRTSAQNIYA
AGDCTDQPQFVYVAAAAGTRAAINMTGGDAALDLSAVPAVVFTDPQVATVGYSEAEAHHDGIETDSRLLTLDNVPRALAN
FDTRGFIKLVADAGSGRLIGVQAVAPEAGELIQTAALAIRNRMTVQELADQLFPYLTMVEGLKLAAQTFSKDVKQLSCCA
G

Sequences:

>Translated_561_residues
MTHLKITGMTCDSCAVHVKDALEKVPGVRSAVVSYPKGTAQIATDPGTSTDALTAAVAGLGYRVMLGEAPPVEKRGNLLD
KALGWLASGEKSAGEGSALRIAVIGSGGAAMAAALKAVEQGAQVTLIERGTIGGTCVNVGCVPSKIMIRAAHIAHLRRES
PFDGGIQATPPAIVRERLLAQQQGRVDELRHAKYEGILASTPAITVLRGEARFQNGHALSVRLAEGGEREVAFDRCLIAT
GASAAVPPIPGLKDTPYWTSNEALASDTLPKRLAVIGSSVVAVELAQAFARLGSQVTVLARSTLFFREDPAIGEAVTAAF
RAEGITVLDHTQASQVAYANGEFVLTTGHGEIRADQLLVATGRAPNTRELALEAAGVRDNAQGAIVIDKAMRTSAQNIYA
AGDCTDQPQFVYVAAAAGTRAAINMTGGDAALDLSAVPAVVFTDPQVATVGYSEAEAHHDGIETDSRLLTLDNVPRALAN
FDTRGFIKLVADAGSGRLIGVQAVAPEAGELIQTAALAIRNRMTVQELADQLFPYLTMVEGLKLAAQTFSKDVKQLSCCA
G
>Mature_560_residues
THLKITGMTCDSCAVHVKDALEKVPGVRSAVVSYPKGTAQIATDPGTSTDALTAAVAGLGYRVMLGEAPPVEKRGNLLDK
ALGWLASGEKSAGEGSALRIAVIGSGGAAMAAALKAVEQGAQVTLIERGTIGGTCVNVGCVPSKIMIRAAHIAHLRRESP
FDGGIQATPPAIVRERLLAQQQGRVDELRHAKYEGILASTPAITVLRGEARFQNGHALSVRLAEGGEREVAFDRCLIATG
ASAAVPPIPGLKDTPYWTSNEALASDTLPKRLAVIGSSVVAVELAQAFARLGSQVTVLARSTLFFREDPAIGEAVTAAFR
AEGITVLDHTQASQVAYANGEFVLTTGHGEIRADQLLVATGRAPNTRELALEAAGVRDNAQGAIVIDKAMRTSAQNIYAA
GDCTDQPQFVYVAAAAGTRAAINMTGGDAALDLSAVPAVVFTDPQVATVGYSEAEAHHDGIETDSRLLTLDNVPRALANF
DTRGFIKLVADAGSGRLIGVQAVAPEAGELIQTAALAIRNRMTVQELADQLFPYLTMVEGLKLAAQTFSKDVKQLSCCAG

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=476, Percent_Identity=29.6218487394958, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI50301238, Length=438, Percent_Identity=29.2237442922374, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI22035672, Length=443, Percent_Identity=28.6681715575621, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI148277071, Length=421, Percent_Identity=28.0285035629454, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI148277065, Length=421, Percent_Identity=27.5534441805226, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI33519430, Length=421, Percent_Identity=28.0285035629454, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI33519428, Length=421, Percent_Identity=28.0285035629454, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI33519426, Length=421, Percent_Identity=28.0285035629454, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI291045266, Length=419, Percent_Identity=27.6849642004773, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI291045268, Length=413, Percent_Identity=26.634382566586, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI87081717, Length=447, Percent_Identity=31.7673378076063, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1786307, Length=470, Percent_Identity=28.936170212766, Blast_Score=189, Evalue=5e-49,
Organism=Escherichia coli, GI1789915, Length=427, Percent_Identity=32.3185011709602, Blast_Score=179, Evalue=5e-46,
Organism=Escherichia coli, GI87082354, Length=460, Percent_Identity=23.2608695652174, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI1789065, Length=230, Percent_Identity=26.9565217391304, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=461, Percent_Identity=27.114967462039, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI71983429, Length=350, Percent_Identity=32.2857142857143, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71983419, Length=350, Percent_Identity=32.2857142857143, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17557007, Length=492, Percent_Identity=26.0162601626016, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=416, Percent_Identity=23.0769230769231, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17559934, Length=267, Percent_Identity=26.2172284644195, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=468, Percent_Identity=28.6324786324786, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6325166, Length=444, Percent_Identity=26.5765765765766, Blast_Score=131, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6325240, Length=472, Percent_Identity=25.4237288135593, Blast_Score=124, Evalue=3e-29,
Organism=Drosophila melanogaster, GI21358499, Length=483, Percent_Identity=31.6770186335404, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI24640553, Length=499, Percent_Identity=29.2585170340681, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24640549, Length=499, Percent_Identity=29.2585170340681, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24640551, Length=499, Percent_Identity=29.2585170340681, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17737741, Length=528, Percent_Identity=27.6515151515151, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000815
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00403 HMA; PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 58373; Mature: 58242

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS01047 HMA_1 ; PS50846 HMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHLKITGMTCDSCAVHVKDALEKVPGVRSAVVSYPKGTAQIATDPGTSTDALTAAVAGL
CCEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCC
GYRVMLGEAPPVEKRGNLLDKALGWLASGEKSAGEGSALRIAVIGSGGAAMAAALKAVEQ
CEEEEECCCCCHHHHCCHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHC
GAQVTLIERGTIGGTCVNVGCVPSKIMIRAAHIAHLRRESPFDGGIQATPPAIVRERLLA
CCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
QQQGRVDELRHAKYEGILASTPAITVLRGEARFQNGHALSVRLAEGGEREVAFDRCLIAT
HHCCCHHHHHHHHHCCEEECCCEEEEEECCCEECCCCEEEEEECCCCCCCCHHHEEEEEC
GASAAVPPIPGLKDTPYWTSNEALASDTLPKRLAVIGSSVVAVELAQAFARLGSQVTVLA
CCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEE
RSTLFFREDPAIGEAVTAAFRAEGITVLDHTQASQVAYANGEFVLTTGHGEIRADQLLVA
EEEEEEECCCCHHHHHHHHHHHCCEEEEECCCCCEEEEECCCEEEECCCCCEEECEEEEE
TGRAPNTRELALEAAGVRDNAQGAIVIDKAMRTSAQNIYAAGDCTDQPQFVYVAAAAGTR
ECCCCCCHHEEEEECCCCCCCCCEEEEEHHHHCCCCCEEECCCCCCCCCEEEEEECCCCE
AAINMTGGDAALDLSAVPAVVFTDPQVATVGYSEAEAHHDGIETDSRLLTLDNVPRALAN
EEEEECCCCCEEECCCCCEEEECCCCEEEECCCHHHHHCCCCCCCCEEEEECCCCHHHHC
FDTRGFIKLVADAGSGRLIGVQAVAPEAGELIQTAALAIRNRMTVQELADQLFPYLTMVE
CCCCCEEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
GLKLAAQTFSKDVKQLSCCAG
HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
THLKITGMTCDSCAVHVKDALEKVPGVRSAVVSYPKGTAQIATDPGTSTDALTAAVAGL
CEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCC
GYRVMLGEAPPVEKRGNLLDKALGWLASGEKSAGEGSALRIAVIGSGGAAMAAALKAVEQ
CEEEEECCCCCHHHHCCHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHC
GAQVTLIERGTIGGTCVNVGCVPSKIMIRAAHIAHLRRESPFDGGIQATPPAIVRERLLA
CCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
QQQGRVDELRHAKYEGILASTPAITVLRGEARFQNGHALSVRLAEGGEREVAFDRCLIAT
HHCCCHHHHHHHHHCCEEECCCEEEEEECCCEECCCCEEEEEECCCCCCCCHHHEEEEEC
GASAAVPPIPGLKDTPYWTSNEALASDTLPKRLAVIGSSVVAVELAQAFARLGSQVTVLA
CCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEE
RSTLFFREDPAIGEAVTAAFRAEGITVLDHTQASQVAYANGEFVLTTGHGEIRADQLLVA
EEEEEEECCCCHHHHHHHHHHHCCEEEEECCCCCEEEEECCCEEEECCCCCEEECEEEEE
TGRAPNTRELALEAAGVRDNAQGAIVIDKAMRTSAQNIYAAGDCTDQPQFVYVAAAAGTR
ECCCCCCHHEEEEECCCCCCCCCEEEEEHHHHCCCCCEEECCCCCCCCCEEEEEECCCCE
AAINMTGGDAALDLSAVPAVVFTDPQVATVGYSEAEAHHDGIETDSRLLTLDNVPRALAN
EEEEECCCCCEEECCCCCEEEECCCCEEEECCCHHHHHCCCCCCCCEEEEECCCCHHHHC
FDTRGFIKLVADAGSGRLIGVQAVAPEAGELIQTAALAIRNRMTVQELADQLFPYLTMVE
CCCCCEEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
GLKLAAQTFSKDVKQLSCCAG
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8302940 [H]