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The map label for this gene is cusS [H]

Identifier: 187928864

GI number: 187928864

Start: 1878565

End: 1879962

Strand: Direct

Name: cusS [H]

Synonym: Rpic_1781

Alternate gene names: 187928864

Gene position: 1878565-1879962 (Clockwise)

Preceding gene: 187928863

Following gene: 187928865

Centisome position: 47.65

GC content: 63.66

Gene sequence:

>1398_bases
ATGGGTCGGTTCTCCCTCACGACGCGGCTGACCGCGTATTTCTCGCTGTGTTCGGCTGCCGTCTTGCTGGGGCTGGGTGT
CGTGATTGCGCTGGCTATGGACCAACACTTCGCGGTGGAGGACTACACCGCGCTGCGTGAGAACGTCAGCGTCATTGAAA
AGATCGTCGAATCGAGCCCCGCCACGCAGGTGCCCGAGCGCATTCGGGAAGCGCTCCAGCACCGCACCGATCTCGTGGCG
CGCATCCAGGGATCCGACCGGCGAATCCTGTACGTGACCAAGGATTTTGATTTCCGGGCTGCGGCGCAAGCGCTGGCGCA
GCTACGGCGCAAGAGCGATGCCCTTGTCTGGGAGCAAGGCGTTCAGCAATATCGGGGCATGCACGCGGCCATCCCCATGC
GAGATGGCTCATCCGGCGCCCTCGATGTCCTGCTGGGTATCAACACGGACATCCACGCCCACTTTCTCCATACGTTCCGT
GGCACGCTGGCTTTCTACATCGCCGTCGCCGCGCTGGCCACCGGCCTGTTCGGATGGTGGGCCGCACGCCATGGCCTGGC
GCCACTGCGCACCATGGCCTCGCGGGCGCAGACCGTGACGGCCGACAAGCTCGACGAGCGCATGCCCGTTGACACCGTGC
CGGTAGAGGTGGCGGACCTTGCCGCCACCCTGAACGCGATGCTGGAGCGGCTGCAGAACGATTTCCGCCGCTTGTCGGAC
TTCTCAACCGACATCGCACATGAACTGCGTACGCCCATCACGAACCTACTCACGCAGACGGAGGTCGTGCTATCCCAGCC
GCGCGAGGGCACCAAGTATCGCGATGTGCTGACCTCCAACGCGGAGGAACTGCAGCGCCTGGCACGCATGGTGTCCGACA
TGCTGTACCTTGCCAAGATGGAGCACAGCCTGACGCTGCCAAGCGCCGAAGACATCCGAATCGCTGACGAAATCCGCGCG
TTGTTTGAGTTCTATGACGCACTCGCCGAAGACAAGGCGGTCCAACTCGAATTGCGCGGTGACGGCCACGTCGTGGGCGA
TCGTCTGATGCTGCGGCGCGCGTTGAGCAATTTGCTGTCCAACGCGATCCGGCATACTCCCCGACTCGGCCACGTGGTCG
TTTCGGTCAGCGACGAACGCGAAGGCGTGACGATTTCCGTCGACAATGACGGAAACGAGATCCCACCGCATCTGCTGCCT
TTCCTCTTTGAGCGCTTCTTCCGCGCGGACAAGTCACGCGCGCGGCCCGAGTCAGAAAGCGCTGGCCTCGGGTTGGCCAT
CACCAAGGCGATCGTCGTGGCACACGGCGGCGCCATTACCGTGTCGCGCATCGACGAGCGCACCCGGTTCTCCGTTCGCT
TACCCCATCGCGCCGAGGTGTCCGACGCGCTCGCTTAG

Upstream 100 bases:

>100_bases
GCCGCCTGCGCGCAAAGATCGATGATCACTTCGAGCCGAAGCTGATCCAAACGGTGCGCGGCATGGGTTATGTGCTCGAA
GCACCCGAGGAAGTGGCTTG

Downstream 100 bases:

>100_bases
CTTGGGAACCCTTGCGGGCAGGCGCACATTACCGCCACATGGCGATCCGATTACGATTCGGACAGGTATGGGAGTTTGCG
GCCTATCTGGCACCGCGCCG

Product: heavy metal sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MGRFSLTTRLTAYFSLCSAAVLLGLGVVIALAMDQHFAVEDYTALRENVSVIEKIVESSPATQVPERIREALQHRTDLVA
RIQGSDRRILYVTKDFDFRAAAQALAQLRRKSDALVWEQGVQQYRGMHAAIPMRDGSSGALDVLLGINTDIHAHFLHTFR
GTLAFYIAVAALATGLFGWWAARHGLAPLRTMASRAQTVTADKLDERMPVDTVPVEVADLAATLNAMLERLQNDFRRLSD
FSTDIAHELRTPITNLLTQTEVVLSQPREGTKYRDVLTSNAEELQRLARMVSDMLYLAKMEHSLTLPSAEDIRIADEIRA
LFEFYDALAEDKAVQLELRGDGHVVGDRLMLRRALSNLLSNAIRHTPRLGHVVVSVSDEREGVTISVDNDGNEIPPHLLP
FLFERFFRADKSRARPESESAGLGLAITKAIVVAHGGAITVSRIDERTRFSVRLPHRAEVSDALA

Sequences:

>Translated_465_residues
MGRFSLTTRLTAYFSLCSAAVLLGLGVVIALAMDQHFAVEDYTALRENVSVIEKIVESSPATQVPERIREALQHRTDLVA
RIQGSDRRILYVTKDFDFRAAAQALAQLRRKSDALVWEQGVQQYRGMHAAIPMRDGSSGALDVLLGINTDIHAHFLHTFR
GTLAFYIAVAALATGLFGWWAARHGLAPLRTMASRAQTVTADKLDERMPVDTVPVEVADLAATLNAMLERLQNDFRRLSD
FSTDIAHELRTPITNLLTQTEVVLSQPREGTKYRDVLTSNAEELQRLARMVSDMLYLAKMEHSLTLPSAEDIRIADEIRA
LFEFYDALAEDKAVQLELRGDGHVVGDRLMLRRALSNLLSNAIRHTPRLGHVVVSVSDEREGVTISVDNDGNEIPPHLLP
FLFERFFRADKSRARPESESAGLGLAITKAIVVAHGGAITVSRIDERTRFSVRLPHRAEVSDALA
>Mature_464_residues
GRFSLTTRLTAYFSLCSAAVLLGLGVVIALAMDQHFAVEDYTALRENVSVIEKIVESSPATQVPERIREALQHRTDLVAR
IQGSDRRILYVTKDFDFRAAAQALAQLRRKSDALVWEQGVQQYRGMHAAIPMRDGSSGALDVLLGINTDIHAHFLHTFRG
TLAFYIAVAALATGLFGWWAARHGLAPLRTMASRAQTVTADKLDERMPVDTVPVEVADLAATLNAMLERLQNDFRRLSDF
STDIAHELRTPITNLLTQTEVVLSQPREGTKYRDVLTSNAEELQRLARMVSDMLYLAKMEHSLTLPSAEDIRIADEIRAL
FEFYDALAEDKAVQLELRGDGHVVGDRLMLRRALSNLLSNAIRHTPRLGHVVVSVSDEREGVTISVDNDGNEIPPHLLPF
LFERFFRADKSRARPESESAGLGLAITKAIVVAHGGAITVSRIDERTRFSVRLPHRAEVSDALA

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=472, Percent_Identity=36.228813559322, Blast_Score=278, Evalue=6e-76,
Organism=Escherichia coli, GI1788279, Length=307, Percent_Identity=30.6188925081433, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1786600, Length=227, Percent_Identity=34.8017621145374, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1790346, Length=265, Percent_Identity=28.3018867924528, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790861, Length=343, Percent_Identity=26.2390670553936, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1788393, Length=260, Percent_Identity=30.3846153846154, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787894, Length=315, Percent_Identity=28.8888888888889, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1786912, Length=216, Percent_Identity=26.8518518518519, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1789149, Length=331, Percent_Identity=24.773413897281, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790551, Length=322, Percent_Identity=25.1552795031056, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790436, Length=246, Percent_Identity=28.8617886178862, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1789403, Length=260, Percent_Identity=28.8461538461538, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI145693157, Length=225, Percent_Identity=28.8888888888889, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87081816, Length=239, Percent_Identity=25.1046025104602, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI48994928, Length=214, Percent_Identity=26.1682242990654, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51635; Mature: 51504

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRFSLTTRLTAYFSLCSAAVLLGLGVVIALAMDQHFAVEDYTALRENVSVIEKIVESSP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
ATQVPERIREALQHRTDLVARIQGSDRRILYVTKDFDFRAAAQALAQLRRKSDALVWEQG
CHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHH
VQQYRGMHAAIPMRDGSSGALDVLLGINTDIHAHFLHTFRGTLAFYIAVAALATGLFGWW
HHHHCCCEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AARHGLAPLRTMASRAQTVTADKLDERMPVDTVPVEVADLAATLNAMLERLQNDFRRLSD
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FSTDIAHELRTPITNLLTQTEVVLSQPREGTKYRDVLTSNAEELQRLARMVSDMLYLAKM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
EHSLTLPSAEDIRIADEIRALFEFYDALAEDKAVQLELRGDGHVVGDRLMLRRALSNLLS
HHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
NAIRHTPRLGHVVVSVSDEREGVTISVDNDGNEIPPHLLPFLFERFFRADKSRARPESES
HHHHCCCCCCEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
AGLGLAITKAIVVAHGGAITVSRIDERTRFSVRLPHRAEVSDALA
CCCHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCHHCHHHHCC
>Mature Secondary Structure 
GRFSLTTRLTAYFSLCSAAVLLGLGVVIALAMDQHFAVEDYTALRENVSVIEKIVESSP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
ATQVPERIREALQHRTDLVARIQGSDRRILYVTKDFDFRAAAQALAQLRRKSDALVWEQG
CHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHH
VQQYRGMHAAIPMRDGSSGALDVLLGINTDIHAHFLHTFRGTLAFYIAVAALATGLFGWW
HHHHCCCEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AARHGLAPLRTMASRAQTVTADKLDERMPVDTVPVEVADLAATLNAMLERLQNDFRRLSD
HHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FSTDIAHELRTPITNLLTQTEVVLSQPREGTKYRDVLTSNAEELQRLARMVSDMLYLAKM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
EHSLTLPSAEDIRIADEIRALFEFYDALAEDKAVQLELRGDGHVVGDRLMLRRALSNLLS
HHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
NAIRHTPRLGHVVVSVSDEREGVTISVDNDGNEIPPHLLPFLFERFFRADKSRARPESES
HHHHCCCCCCEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
AGLGLAITKAIVVAHGGAITVSRIDERTRFSVRLPHRAEVSDALA
CCCHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCHHCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]