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The map label for this gene is rnhB

Identifier: 187928381

GI number: 187928381

Start: 1388087

End: 1388785

Strand: Direct

Name: rnhB

Synonym: Rpic_1292

Alternate gene names: 187928381

Gene position: 1388087-1388785 (Clockwise)

Preceding gene: 187928380

Following gene: 187928382

Centisome position: 35.21

GC content: 65.52

Gene sequence:

>699_bases
ATGGCTTCCCGCAAACCCAATCCGGACCAGCTTGGCCTGCTCCTCTCGCAGGAGCCGGCGGCCAAGCGCGCGCGTCGCAT
TCTCTGTGGCGTGGATGAGGCTGGACGTGGGCCGTTGGCCGGGCCGGTGACGGCAGCGGCGGTCGTTCTCAATCCGCGCA
AGCCGATCAAGGGCTTGGCTGATTCTAAGATCCTGACCGCCAGGAAGCGTGAGGCGCTGTACGACGAGATTGTCGAAAAG
GCGCTCGCCTGGCACGTGGCGGAAGCCACCGTCGAAGAAATTGATCGCATCAACATCCTGCACGCGACCATGCTGGCCAT
GCAGCGTGCGGTACAGGGCGTGGCCGCATTGGGCACGCTTCCGGATCTCGTGCAGGTGGACGGCAATCGCTGCCCACAGG
TGGCGTTTGCGGTGGAGGCCATCGTCAAGGGCGATGCGCTGGTGCCGGCCATTTCGGCGGCCTCCATCCTGGCGAAGGTG
ACGCGCGACCGCCAGTTGGCCGCGCTGCATATCGAGTTTCCGCTCTACGGGTTTGACGTTCATGCCGGCTATGGCACGCC
GCAGCATCTGGCGGCGATCGACCTGCATGGCGTGACGCCGCATCATCGCCGCTCGTTCGCGCCCGTGCGTCGTGCGTTGG
AGGGGATGAGCGCCATCAGCATCGAAACCGTCGCTGCAGGCAGCGCCTGGGACGACTGA

Upstream 100 bases:

>100_bases
CACTTCACGCAGATGCACCTGACGCTCAAGCAGAATATGGCGGAGATCGGCGCGAAAGTCGTGGTCGATCTGCTACGCAG
TCGCGGTAAGCTTTAAGCCC

Downstream 100 bases:

>100_bases
CCTTTTTCCGCTGGGCCCATTCGTGAAGCACATCACTTCCCGCGACAACGCGGTGTTCAAGCATCTCAAGGCGCTCTCGG
GCTCGACGCAGCACCGTCGG

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MASRKPNPDQLGLLLSQEPAAKRARRILCGVDEAGRGPLAGPVTAAAVVLNPRKPIKGLADSKILTARKREALYDEIVEK
ALAWHVAEATVEEIDRINILHATMLAMQRAVQGVAALGTLPDLVQVDGNRCPQVAFAVEAIVKGDALVPAISAASILAKV
TRDRQLAALHIEFPLYGFDVHAGYGTPQHLAAIDLHGVTPHHRRSFAPVRRALEGMSAISIETVAAGSAWDD

Sequences:

>Translated_232_residues
MASRKPNPDQLGLLLSQEPAAKRARRILCGVDEAGRGPLAGPVTAAAVVLNPRKPIKGLADSKILTARKREALYDEIVEK
ALAWHVAEATVEEIDRINILHATMLAMQRAVQGVAALGTLPDLVQVDGNRCPQVAFAVEAIVKGDALVPAISAASILAKV
TRDRQLAALHIEFPLYGFDVHAGYGTPQHLAAIDLHGVTPHHRRSFAPVRRALEGMSAISIETVAAGSAWDD
>Mature_231_residues
ASRKPNPDQLGLLLSQEPAAKRARRILCGVDEAGRGPLAGPVTAAAVVLNPRKPIKGLADSKILTARKREALYDEIVEKA
LAWHVAEATVEEIDRINILHATMLAMQRAVQGVAALGTLPDLVQVDGNRCPQVAFAVEAIVKGDALVPAISAASILAKVT
RDRQLAALHIEFPLYGFDVHAGYGTPQHLAAIDLHGVTPHHRRSFAPVRRALEGMSAISIETVAAGSAWDD

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=188, Percent_Identity=59.5744680851064, Blast_Score=219, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_RALPJ (B2UBB5)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001898868.1
- ProteinModelPortal:   B2UBB5
- SMR:   B2UBB5
- GeneID:   6288926
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_1292
- HOGENOM:   HBG584843
- OMA:   IICGVDE
- ProtClustDB:   PRK00015
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 24725; Mature: 24594

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASRKPNPDQLGLLLSQEPAAKRARRILCGVDEAGRGPLAGPVTAAAVVLNPRKPIKGLA
CCCCCCCHHHHCEEECCCHHHHHHHHHHCCCCCCCCCCCCCCHHHEEEEECCCCCCCCCC
DSKILTARKREALYDEIVEKALAWHVAEATVEEIDRINILHATMLAMQRAVQGVAALGTL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDLVQVDGNRCPQVAFAVEAIVKGDALVPAISAASILAKVTRDRQLAALHIEFPLYGFDV
CHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEE
HAGYGTPQHLAAIDLHGVTPHHRRSFAPVRRALEGMSAISIETVAAGSAWDD
ECCCCCHHHEEEEEECCCCCHHHHHHHHHHHHHHCCHHHEEEEEECCCCCCC
>Mature Secondary Structure 
ASRKPNPDQLGLLLSQEPAAKRARRILCGVDEAGRGPLAGPVTAAAVVLNPRKPIKGLA
CCCCCCHHHHCEEECCCHHHHHHHHHHCCCCCCCCCCCCCCHHHEEEEECCCCCCCCCC
DSKILTARKREALYDEIVEKALAWHVAEATVEEIDRINILHATMLAMQRAVQGVAALGTL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDLVQVDGNRCPQVAFAVEAIVKGDALVPAISAASILAKVTRDRQLAALHIEFPLYGFDV
CHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEE
HAGYGTPQHLAAIDLHGVTPHHRRSFAPVRRALEGMSAISIETVAAGSAWDD
ECCCCCHHHEEEEEECCCCCHHHHHHHHHHHHHHCCHHHEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA