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The map label for this gene is iaaA [H]

Identifier: 187928340

GI number: 187928340

Start: 1340077

End: 1341039

Strand: Direct

Name: iaaA [H]

Synonym: Rpic_1251

Alternate gene names: 187928340

Gene position: 1340077-1341039 (Clockwise)

Preceding gene: 187928339

Following gene: 187928341

Centisome position: 33.99

GC content: 66.56

Gene sequence:

>963_bases
ATGACTACGCCCATCCTCGCCATCCACGGCGGCGCCGGCACCATCACCCGCACGGCCATGAGTGCCGACAAGGAAGCCGC
TTACCAGCAAGCGCTGAACGACATCCTGGCCGCAGGGCAACGCATCCTTGTGGAAGGCGGTAGCGCCCTGGATGCCGTGA
CCGAAGCCGTGCGCCTGCTCGAAGAGTGCCCGCTGTTCAACGCCGGCAAGGGCGCGGTGCTTACCAGTGCCGGCACGTAT
GAACTCGATGCGTCGATCATGGATGGCGCCAGGCTGGCCGCCGGCGCAGTGACCTGTGTCAAGCGCCTGCGCAACCCGAT
CCTCGCGGCCCGCGCGGTGATGGAGCGCAGCGAACACGTGCTGTTCACCTCCGAAGGCGCCGAGGCTTTCGCGAAAGCGC
AGGGCCTGGAGTTTGTCGAGCCGGACTACTACTACACCGAAGCGCGCTACGCCCAATGGCAGCGCGCCCGCCAGCAAGAC
GGCATGGCGCTGCTCGATCACGATGCCGCGTCGCTCGTGGCCAAGGAAGCTGCACCCATCGACCCGGACAACAAGTTCGG
CACCGTGGGCGCCGTGGCATGCGACGCGCAAGGGCGCCTCGCGGCGGCCACCTCCACGGGCGGCGTGACCAACAAGAAGG
TCGGCCGCGTGGGCGATACGCCCATCGTTGGCGCGGGTTGCTTTGCAAACAACGTGGCGGCGGTGTCGTGCACCGGCACG
GGCGAGATGTTCATCCGCGCCGTGGCTGCCTACGACGTGGCCGCACAGATGGAATACGCCGGCAAGTCACTGGCCGATGC
AACCGACGACGTGGTCATGCGCAAGCTCATGGCCATCAATGGCCGCGGTGGGCTGATTGCCGTAGACGCGAAGGGCAACG
TCGCGTTGCCGTTCAACACCGAAGGGATGTACCGCGGGTTCGCACGCGGCGCGCAAGCGCCGGTTGTGTCGATCTATCGG
TAA

Upstream 100 bases:

>100_bases
CGCCGCGCACCAATGACGCCCCGGGCGCCTCCGGCACACCGACAACCACCCGACGCGGCCGCAAACGCGCCGCTGAAAAC
TGATTGACATCGAGCCACGC

Downstream 100 bases:

>100_bases
GAAGCCGAAGCCGCCAAGCAGGACAGCAGGAGATTTGTCGTGACAGCAACCAAACAGCCGAGTGCCAGCAGCGTCATTGC
CATGCCCGAGCAGCGCGTTG

Product: peptidase T2 asparaginase 2

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MTTPILAIHGGAGTITRTAMSADKEAAYQQALNDILAAGQRILVEGGSALDAVTEAVRLLEECPLFNAGKGAVLTSAGTY
ELDASIMDGARLAAGAVTCVKRLRNPILAARAVMERSEHVLFTSEGAEAFAKAQGLEFVEPDYYYTEARYAQWQRARQQD
GMALLDHDAASLVAKEAAPIDPDNKFGTVGAVACDAQGRLAAATSTGGVTNKKVGRVGDTPIVGAGCFANNVAAVSCTGT
GEMFIRAVAAYDVAAQMEYAGKSLADATDDVVMRKLMAINGRGGLIAVDAKGNVALPFNTEGMYRGFARGAQAPVVSIYR

Sequences:

>Translated_320_residues
MTTPILAIHGGAGTITRTAMSADKEAAYQQALNDILAAGQRILVEGGSALDAVTEAVRLLEECPLFNAGKGAVLTSAGTY
ELDASIMDGARLAAGAVTCVKRLRNPILAARAVMERSEHVLFTSEGAEAFAKAQGLEFVEPDYYYTEARYAQWQRARQQD
GMALLDHDAASLVAKEAAPIDPDNKFGTVGAVACDAQGRLAAATSTGGVTNKKVGRVGDTPIVGAGCFANNVAAVSCTGT
GEMFIRAVAAYDVAAQMEYAGKSLADATDDVVMRKLMAINGRGGLIAVDAKGNVALPFNTEGMYRGFARGAQAPVVSIYR
>Mature_319_residues
TTPILAIHGGAGTITRTAMSADKEAAYQQALNDILAAGQRILVEGGSALDAVTEAVRLLEECPLFNAGKGAVLTSAGTYE
LDASIMDGARLAAGAVTCVKRLRNPILAARAVMERSEHVLFTSEGAEAFAKAQGLEFVEPDYYYTEARYAQWQRARQQDG
MALLDHDAASLVAKEAAPIDPDNKFGTVGAVACDAQGRLAAATSTGGVTNKKVGRVGDTPIVGAGCFANNVAAVSCTGTG
EMFIRAVAAYDVAAQMEYAGKSLADATDDVVMRKLMAINGRGGLIAVDAKGNVALPFNTEGMYRGFARGAQAPVVSIYR

Specific function: Degrades proteins damaged by L-isoaspartyl residue formation (also known as beta-Asp residues). Degrades L- isoaspartyl-containing di- and tripeptides. Acts best on iso-Asp- Leu, followed by iso-Asp-Ala, -His and to a lesser extent iso-Asp- Lys, -Phe and

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=303, Percent_Identity=39.6039603960396, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI145275200, Length=303, Percent_Identity=39.6039603960396, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI98991769, Length=286, Percent_Identity=32.8671328671329, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI285002251, Length=238, Percent_Identity=33.6134453781513, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI285002253, Length=238, Percent_Identity=31.5126050420168, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1787050, Length=321, Percent_Identity=59.5015576323988, Blast_Score=362, Evalue=1e-101,
Organism=Drosophila melanogaster, GI18921183, Length=315, Percent_Identity=36.5079365079365, Blast_Score=167, Evalue=7e-42,
Organism=Drosophila melanogaster, GI21357893, Length=269, Percent_Identity=31.5985130111524, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI221330102, Length=247, Percent_Identity=31.9838056680162, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24652135, Length=247, Percent_Identity=31.9838056680162, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24655553, Length=222, Percent_Identity=30.1801801801802, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24657358, Length=293, Percent_Identity=25.5972696245734, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 33318; Mature: 33187

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPILAIHGGAGTITRTAMSADKEAAYQQALNDILAAGQRILVEGGSALDAVTEAVRLL
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHH
EECPLFNAGKGAVLTSAGTYELDASIMDGARLAAGAVTCVKRLRNPILAARAVMERSEHV
HHCCCCCCCCCCEEECCCCEEECHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LFTSEGAEAFAKAQGLEFVEPDYYYTEARYAQWQRARQQDGMALLDHDAASLVAKEAAPI
EEECCCHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCC
DPDNKFGTVGAVACDAQGRLAAATSTGGVTNKKVGRVGDTPIVGAGCFANNVAAVSCTGT
CCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCEEECCHHHCCEEEEEECCC
GEMFIRAVAAYDVAAQMEYAGKSLADATDDVVMRKLMAINGRGGLIAVDAKGNVALPFNT
HHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECC
EGMYRGFARGAQAPVVSIYR
CHHHHHHHCCCCCCEEEECC
>Mature Secondary Structure 
TTPILAIHGGAGTITRTAMSADKEAAYQQALNDILAAGQRILVEGGSALDAVTEAVRLL
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHH
EECPLFNAGKGAVLTSAGTYELDASIMDGARLAAGAVTCVKRLRNPILAARAVMERSEHV
HHCCCCCCCCCCEEECCCCEEECHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LFTSEGAEAFAKAQGLEFVEPDYYYTEARYAQWQRARQQDGMALLDHDAASLVAKEAAPI
EEECCCHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCC
DPDNKFGTVGAVACDAQGRLAAATSTGGVTNKKVGRVGDTPIVGAGCFANNVAAVSCTGT
CCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCEEECCHHHCCEEEEEECCC
GEMFIRAVAAYDVAAQMEYAGKSLADATDDVVMRKLMAINGRGGLIAVDAKGNVALPFNT
HHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECC
EGMYRGFARGAQAPVVSIYR
CHHHHHHHCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]