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The map label for this gene is cysW [H]

Identifier: 187928311

GI number: 187928311

Start: 1310380

End: 1311360

Strand: Direct

Name: cysW [H]

Synonym: Rpic_1222

Alternate gene names: 187928311

Gene position: 1310380-1311360 (Clockwise)

Preceding gene: 187928310

Following gene: 187928312

Centisome position: 33.24

GC content: 63.3

Gene sequence:

>981_bases
ATGGCCGGTACTGTTTCCCGTCAACTTGGTCAGGCGCCCGCTGTTGGCCGCCGTGGGGCCACCGCAGAATCCGCTTGGGT
GCGCGCCACGCTGATCCTCATATCGTTCGTGTTCCTGGCGCTGTTCCTGTTCGTGCCGCTGGCGAGCGTGTTCTACGAGG
CGTTCAAGAAAGGCATCGACGTCTACCTTGAAGCGCTGATCGAGCCCGATGCGCTGTCCGCGATTTACCTGACACTGACG
GTCGCGGCGATTGCGGTGCCGCTGAACGTGGTGTTTGGCGTAGCCGCGGCGTGGGCGATCGCCAAGTTCGAGTTTCGCGG
CAAGAACCTGCTGCTCACGCTGATCGACTTGCCGTTCTCGGTATCGCCCGTCATTGCCGGCCTGATCTACGTGCTGCTGT
TCGGCGCGCAGGGCTGGTTCGGGCCTTACCTGTCCGACCACGACTTCAAGGTCATCTTTGCTGTGCCGGGCATCGTGCTG
GCCACGGTGTTCGTCACGTTTCCGTTTGTGGCGCGCGAGCTGATTCCGCTCATGCAGGCGCAGGGCAGCGAAGAGGAAGA
GGCCGCCATCGTGCTCGGCGCCTCGGGCTGGCAGACGTTCTTTCGCGTGACGCTGCCGAACATCAAGTGGGGCCTGTTGT
ACGGCGTGATCCTCTGCAACGCGCGGGCGATGGGCGAGTTTGGCGCGGTGTCGGTGGTGTCTGGCCACATTCGCGGGCTG
ACCAACACGATGCCGCTGCACGTGGAAATTCTCTACAACGAATACAACTTTGCGGCCGCGTTTGCCGTGGCGTCGTTGCT
GACGTTGCTGGCGCTTGTGACGCTGGCACTGAAGACGCTGGTCGAATGGCGCAGCCGCCGCGAGCTGGCAGACGCAGATG
CCGGCGTGGGCGAGGGGCCGGGCCAGCGCGTTACGCAACAAACACCGATTTCGAATTCGCACGTCGCCGTGCAATCTCCG
CTCGAAGGGAGCGCAGCATGA

Upstream 100 bases:

>100_bases
AACCTGCTGCAGGCCTGGACGCGCCGCCGCGGCAATCGCAACGACACCATCGAGCGCGCCCCTGAACCTTCGACCGCCCA
CGCGGCAGCTGGAGCCTGAC

Downstream 100 bases:

>100_bases
GCATCCAGGTTCAACACATCACCAAGCGCTTTGGCAACTTCGTTGCCCTCGACGATGTTTCGCTCGACTTCAAGCAGGGC
GAACTGACCGCGCTGCTGGG

Product: sulfate ABC transporter inner membrane subunit CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MAGTVSRQLGQAPAVGRRGATAESAWVRATLILISFVFLALFLFVPLASVFYEAFKKGIDVYLEALIEPDALSAIYLTLT
VAAIAVPLNVVFGVAAAWAIAKFEFRGKNLLLTLIDLPFSVSPVIAGLIYVLLFGAQGWFGPYLSDHDFKVIFAVPGIVL
ATVFVTFPFVARELIPLMQAQGSEEEEAAIVLGASGWQTFFRVTLPNIKWGLLYGVILCNARAMGEFGAVSVVSGHIRGL
TNTMPLHVEILYNEYNFAAAFAVASLLTLLALVTLALKTLVEWRSRRELADADAGVGEGPGQRVTQQTPISNSHVAVQSP
LEGSAA

Sequences:

>Translated_326_residues
MAGTVSRQLGQAPAVGRRGATAESAWVRATLILISFVFLALFLFVPLASVFYEAFKKGIDVYLEALIEPDALSAIYLTLT
VAAIAVPLNVVFGVAAAWAIAKFEFRGKNLLLTLIDLPFSVSPVIAGLIYVLLFGAQGWFGPYLSDHDFKVIFAVPGIVL
ATVFVTFPFVARELIPLMQAQGSEEEEAAIVLGASGWQTFFRVTLPNIKWGLLYGVILCNARAMGEFGAVSVVSGHIRGL
TNTMPLHVEILYNEYNFAAAFAVASLLTLLALVTLALKTLVEWRSRRELADADAGVGEGPGQRVTQQTPISNSHVAVQSP
LEGSAA
>Mature_325_residues
AGTVSRQLGQAPAVGRRGATAESAWVRATLILISFVFLALFLFVPLASVFYEAFKKGIDVYLEALIEPDALSAIYLTLTV
AAIAVPLNVVFGVAAAWAIAKFEFRGKNLLLTLIDLPFSVSPVIAGLIYVLLFGAQGWFGPYLSDHDFKVIFAVPGIVLA
TVFVTFPFVARELIPLMQAQGSEEEEAAIVLGASGWQTFFRVTLPNIKWGLLYGVILCNARAMGEFGAVSVVSGHIRGLT
NTMPLHVEILYNEYNFAAAFAVASLLTLLALVTLALKTLVEWRSRRELADADAGVGEGPGQRVTQQTPISNSHVAVQSPL
EGSAA

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=263, Percent_Identity=54.3726235741445, Blast_Score=302, Evalue=2e-83,
Organism=Escherichia coli, GI1788764, Length=206, Percent_Identity=38.3495145631068, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1786980, Length=230, Percent_Identity=32.6086956521739, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1787369, Length=230, Percent_Identity=27.8260869565217, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34959; Mature: 34828

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGTVSRQLGQAPAVGRRGATAESAWVRATLILISFVFLALFLFVPLASVFYEAFKKGID
CCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VYLEALIEPDALSAIYLTLTVAAIAVPLNVVFGVAAAWAIAKFEFRGKNLLLTLIDLPFS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEECCCCC
VSPVIAGLIYVLLFGAQGWFGPYLSDHDFKVIFAVPGIVLATVFVTFPFVARELIPLMQA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHC
QGSEEEEAAIVLGASGWQTFFRVTLPNIKWGLLYGVILCNARAMGEFGAVSVVSGHIRGL
CCCCCCCCEEEEECCCHHEEEEEECCCCHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHCC
TNTMPLHVEILYNEYNFAAAFAVASLLTLLALVTLALKTLVEWRSRRELADADAGVGEGP
CCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GQRVTQQTPISNSHVAVQSPLEGSAA
CCCCCCCCCCCCCCEEEECCCCCCCC
>Mature Secondary Structure 
AGTVSRQLGQAPAVGRRGATAESAWVRATLILISFVFLALFLFVPLASVFYEAFKKGID
CCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VYLEALIEPDALSAIYLTLTVAAIAVPLNVVFGVAAAWAIAKFEFRGKNLLLTLIDLPFS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEECCCCC
VSPVIAGLIYVLLFGAQGWFGPYLSDHDFKVIFAVPGIVLATVFVTFPFVARELIPLMQA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHC
QGSEEEEAAIVLGASGWQTFFRVTLPNIKWGLLYGVILCNARAMGEFGAVSVVSGHIRGL
CCCCCCCCEEEEECCCHHEEEEEECCCCHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHCC
TNTMPLHVEILYNEYNFAAAFAVASLLTLLALVTLALKTLVEWRSRRELADADAGVGEGP
CCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GQRVTQQTPISNSHVAVQSPLEGSAA
CCCCCCCCCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]