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The map label for this gene is cysU [H]

Identifier: 187928310

GI number: 187928310

Start: 1309464

End: 1310378

Strand: Direct

Name: cysU [H]

Synonym: Rpic_1221

Alternate gene names: 187928310

Gene position: 1309464-1310378 (Clockwise)

Preceding gene: 187928309

Following gene: 187928311

Centisome position: 33.21

GC content: 65.03

Gene sequence:

>915_bases
ATGAGCCTGCCTTCCGCGTTTTTTTCGTCCAAGCGGCGTAAGCCCGCCAACGTGCTGCCCGGCTTCGGGCTGGCGCTGGG
CTTTTCGCTTCTGTACCTCGCGCTGATCGTCCTGATCCCGCTGTCGGCAACCGTCCTCAAGACGTTCACCATGACGTGGG
ATGCGTTCTGGACCACCGTCACCGCGCCGCGCGTGGTGGCGTCGTACAAGCTGACCTTTGGCGCCTCGCTGATCGCTGCG
GTGATCAACCTCGTGTTCGGGCTGATCGTGGCGTGGGTGCTGGTGCGCTATCGCTTTCCGGGCAAGCGGTTGATCGACGC
GCTGGTGGATCTGCCTTTTGCGCTGCCGACGGCCGTGGCTGGTATCGCGCTCACCGCGTTGTATGCGCCGAATGGCTGGA
TCGGCTCGCGGCTGGAGCCGCTGGGGCTGAAGGTGGCGTTCACGCCGCTGGGCATCGTGGTGGCGCTCACGTTCATCGGC
TTGCCGTTTGTCGTGCGCACGGTGCAGCCGGTGCTGGAAGATCTGGAGACCGAACTGGAAGAGGCGGCTGCCAGCCTGGG
TGCCACGCGCTGGCAGACCTTCATTCGCGTGATCCTGCCGACCTTGCTGCCTGCATTGCTGACCGGTTTCGCGCTGGCGT
TTGCGCGGGCCACGGGCGAGTACGGCTCGGTCATCTTCATCGCCGGCAACATGCCGATGGTCTCCGAGATCACGCCGCTG
ATGATCTATTCCAAGCTGGAGCAGTTCGACTACGCGGGCGCCACGGCCATTGCCGTCGTCATGCTCGGCATCTCGTTCAC
GCTGTTGCTGGTGATCAACCTGCTGCAGGCCTGGACGCGCCGCCGCGGCAATCGCAACGACACCATCGAGCGCGCCCCTG
AACCTTCGACCGCCCACGCGGCAGCTGGAGCCTGA

Upstream 100 bases:

>100_bases
GCAAATATTCGTTTTCGTCATAAGAACAGTCCGCCACAATTGGTTCATCTGCATATCGGCCGCAGTGTTTTGCTCTGCGG
CCATTTTTATCGGTGCGCTG

Downstream 100 bases:

>100_bases
CATGGCCGGTACTGTTTCCCGTCAACTTGGTCAGGCGCCCGCTGTTGGCCGCCGTGGGGCCACCGCAGAATCCGCTTGGG
TGCGCGCCACGCTGATCCTC

Product: sulfate ABC transporter inner membrane subunit CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MSLPSAFFSSKRRKPANVLPGFGLALGFSLLYLALIVLIPLSATVLKTFTMTWDAFWTTVTAPRVVASYKLTFGASLIAA
VINLVFGLIVAWVLVRYRFPGKRLIDALVDLPFALPTAVAGIALTALYAPNGWIGSRLEPLGLKVAFTPLGIVVALTFIG
LPFVVRTVQPVLEDLETELEEAAASLGATRWQTFIRVILPTLLPALLTGFALAFARATGEYGSVIFIAGNMPMVSEITPL
MIYSKLEQFDYAGATAIAVVMLGISFTLLLVINLLQAWTRRRGNRNDTIERAPEPSTAHAAAGA

Sequences:

>Translated_304_residues
MSLPSAFFSSKRRKPANVLPGFGLALGFSLLYLALIVLIPLSATVLKTFTMTWDAFWTTVTAPRVVASYKLTFGASLIAA
VINLVFGLIVAWVLVRYRFPGKRLIDALVDLPFALPTAVAGIALTALYAPNGWIGSRLEPLGLKVAFTPLGIVVALTFIG
LPFVVRTVQPVLEDLETELEEAAASLGATRWQTFIRVILPTLLPALLTGFALAFARATGEYGSVIFIAGNMPMVSEITPL
MIYSKLEQFDYAGATAIAVVMLGISFTLLLVINLLQAWTRRRGNRNDTIERAPEPSTAHAAAGA
>Mature_303_residues
SLPSAFFSSKRRKPANVLPGFGLALGFSLLYLALIVLIPLSATVLKTFTMTWDAFWTTVTAPRVVASYKLTFGASLIAAV
INLVFGLIVAWVLVRYRFPGKRLIDALVDLPFALPTAVAGIALTALYAPNGWIGSRLEPLGLKVAFTPLGIVVALTFIGL
PFVVRTVQPVLEDLETELEEAAASLGATRWQTFIRVILPTLLPALLTGFALAFARATGEYGSVIFIAGNMPMVSEITPLM
IYSKLEQFDYAGATAIAVVMLGISFTLLLVINLLQAWTRRRGNRNDTIERAPEPSTAHAAAGA

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=276, Percent_Identity=55.0724637681159, Blast_Score=268, Evalue=3e-73,
Organism=Escherichia coli, GI87082099, Length=284, Percent_Identity=31.6901408450704, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI1786980, Length=217, Percent_Identity=35.0230414746544, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1786254, Length=223, Percent_Identity=29.1479820627803, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787369, Length=155, Percent_Identity=30.9677419354839, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787368, Length=241, Percent_Identity=28.2157676348548, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32725; Mature: 32594

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPSAFFSSKRRKPANVLPGFGLALGFSLLYLALIVLIPLSATVLKTFTMTWDAFWTTV
CCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAPRVVASYKLTFGASLIAAVINLVFGLIVAWVLVRYRFPGKRLIDALVDLPFALPTAVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHH
GIALTALYAPNGWIGSRLEPLGLKVAFTPLGIVVALTFIGLPFVVRTVQPVLEDLETELE
HHHHHHHCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAAASLGATRWQTFIRVILPTLLPALLTGFALAFARATGEYGSVIFIAGNMPMVSEITPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHH
MIYSKLEQFDYAGATAIAVVMLGISFTLLLVINLLQAWTRRRGNRNDTIERAPEPSTAHA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
AAGA
CCCC
>Mature Secondary Structure 
SLPSAFFSSKRRKPANVLPGFGLALGFSLLYLALIVLIPLSATVLKTFTMTWDAFWTTV
CCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAPRVVASYKLTFGASLIAAVINLVFGLIVAWVLVRYRFPGKRLIDALVDLPFALPTAVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHH
GIALTALYAPNGWIGSRLEPLGLKVAFTPLGIVVALTFIGLPFVVRTVQPVLEDLETELE
HHHHHHHCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAAASLGATRWQTFIRVILPTLLPALLTGFALAFARATGEYGSVIFIAGNMPMVSEITPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHH
MIYSKLEQFDYAGATAIAVVMLGISFTLLLVINLLQAWTRRRGNRNDTIERAPEPSTAHA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
AAGA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]