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The map label for this gene is dusA [H]

Identifier: 187928263

GI number: 187928263

Start: 1263605

End: 1264588

Strand: Direct

Name: dusA [H]

Synonym: Rpic_1174

Alternate gene names: 187928263

Gene position: 1263605-1264588 (Clockwise)

Preceding gene: 187928259

Following gene: 187928265

Centisome position: 32.05

GC content: 65.14

Gene sequence:

>984_bases
ATGATGGATTGGACTGACAGACATTGCCGGTTCTTCCATCGCCAGATCTCGCGCCACACGTGGCTCTACACCGAAATGGT
GACCACGGGCGCGCTGCTGCACGGCGACGTCCCGCGCCACCTCGATTTTGACGAGTTCGAGCATCCCGTGGCGCTCCAGC
TCGGCGGCAGTGAACCGGACGATCTGGCCCAGGCCGCGCGCCTCGGCCAGCAATGGGGCTACAAAGAAATCAACCTCAAC
TGCGGCTGCCCGTCGGAGCGCGTGCAGCGCGGTGCGTTCGGCGCCTGCCTGATGGCTGAGCCCAAGCTCGTCGCGCAGGG
TGTCACGGCGATGCGCGATGCGGTGTCGATTCCGGTCACGGTCAAGCACCGCATCGGCATTGATGCAATCGAGACCTACG
ATTTCGTGCGCGATTTCGTCGGCACCATCGCCGAGGCGGGTTGCGAGACGTTCATCGTGCACGCGCGCAATGCCATCCTG
AAAGGCCTGAGCCCGAAGGAAAATCGCGAGATTCCGCCGCTGCGCTACGAAGTCGTCTATCAGCTCAAGCGGGATTTCCC
GCATCTGGAAATTCTGCTCAACGGTGGGGTGCAGACGCTCGAGCAGATCGACACGCACCTGGCGCACGTTGATGGCGTAA
TGATCGGCCGTGAGGCGTATCACCATCCGCATCTGATGGCTGCGTTCGATGCGCGCTACTACGGCGCAGGCACCGAGGCC
CCCACGCGCGCCGAGGTGGAAGACGCGATGATCGGCTACGTCGGCCGCTGGGTAGAGCGTGGCGGCTACGCAGGTGCGGC
GGTGCGGCACATGCTGGGGCTGTATCGCGGTGTGGCGGGCGCGCGCGGCTGGCGGCGCGTGCTGTCGGACTCCAAGGCGC
TGGGCCGCGTCAAGACGCTGGCCGACGCCCATGCACTGTTCGACGAGGCGCGTGCCCACCTGCGGCGTGGCGAAGAATCG
AATGAGGAGGCGGTAGCGGTTTGA

Upstream 100 bases:

>100_bases
CAAATCTCATGAAATCGCGCAGAAAACAGTAGGGTGTAAGCGCTTGAATTCAAACCGTATTCCAATGAAATCAACGCCTA
GGCGGCTGAGCGTGGCCCCG

Downstream 100 bases:

>100_bases
TGCGGGGAAAGGGGCGTTGCCCCGATCGCTGAATGCAAAAAAGCCCGCGAATGCGGGCTTTTTCTTGAAACTTGGGGTGA
CTGATGGGATTCGAACCCAC

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MMDWTDRHCRFFHRQISRHTWLYTEMVTTGALLHGDVPRHLDFDEFEHPVALQLGGSEPDDLAQAARLGQQWGYKEINLN
CGCPSERVQRGAFGACLMAEPKLVAQGVTAMRDAVSIPVTVKHRIGIDAIETYDFVRDFVGTIAEAGCETFIVHARNAIL
KGLSPKENREIPPLRYEVVYQLKRDFPHLEILLNGGVQTLEQIDTHLAHVDGVMIGREAYHHPHLMAAFDARYYGAGTEA
PTRAEVEDAMIGYVGRWVERGGYAGAAVRHMLGLYRGVAGARGWRRVLSDSKALGRVKTLADAHALFDEARAHLRRGEES
NEEAVAV

Sequences:

>Translated_327_residues
MMDWTDRHCRFFHRQISRHTWLYTEMVTTGALLHGDVPRHLDFDEFEHPVALQLGGSEPDDLAQAARLGQQWGYKEINLN
CGCPSERVQRGAFGACLMAEPKLVAQGVTAMRDAVSIPVTVKHRIGIDAIETYDFVRDFVGTIAEAGCETFIVHARNAIL
KGLSPKENREIPPLRYEVVYQLKRDFPHLEILLNGGVQTLEQIDTHLAHVDGVMIGREAYHHPHLMAAFDARYYGAGTEA
PTRAEVEDAMIGYVGRWVERGGYAGAAVRHMLGLYRGVAGARGWRRVLSDSKALGRVKTLADAHALFDEARAHLRRGEES
NEEAVAV
>Mature_327_residues
MMDWTDRHCRFFHRQISRHTWLYTEMVTTGALLHGDVPRHLDFDEFEHPVALQLGGSEPDDLAQAARLGQQWGYKEINLN
CGCPSERVQRGAFGACLMAEPKLVAQGVTAMRDAVSIPVTVKHRIGIDAIETYDFVRDFVGTIAEAGCETFIVHARNAIL
KGLSPKENREIPPLRYEVVYQLKRDFPHLEILLNGGVQTLEQIDTHLAHVDGVMIGREAYHHPHLMAAFDARYYGAGTEA
PTRAEVEDAMIGYVGRWVERGGYAGAAVRHMLGLYRGVAGARGWRRVLSDSKALGRVKTLADAHALFDEARAHLRRGEES
NEEAVAV

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=215, Percent_Identity=26.046511627907, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI40807366, Length=184, Percent_Identity=29.3478260869565, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI145693211, Length=314, Percent_Identity=56.687898089172, Blast_Score=355, Evalue=2e-99,
Organism=Escherichia coli, GI1788462, Length=181, Percent_Identity=30.939226519337, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1789660, Length=176, Percent_Identity=25.5681818181818, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 36505; Mature: 36505

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDWTDRHCRFFHRQISRHTWLYTEMVTTGALLHGDVPRHLDFDEFEHPVALQLGGSEPD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCEEEEECCCCHH
DLAQAARLGQQWGYKEINLNCGCPSERVQRGAFGACLMAEPKLVAQGVTAMRDAVSIPVT
HHHHHHHHHHHCCCEEEEECCCCCHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCCCEE
VKHRIGIDAIETYDFVRDFVGTIAEAGCETFIVHARNAILKGLSPKENREIPPLRYEVVY
EECCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
QLKRDFPHLEILLNGGVQTLEQIDTHLAHVDGVMIGREAYHHPHLMAAFDARYYGAGTEA
HHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHEECHHHHCCCCHHHHHCCHHCCCCCCC
PTRAEVEDAMIGYVGRWVERGGYAGAAVRHMLGLYRGVAGARGWRRVLSDSKALGRVKTL
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
ADAHALFDEARAHLRRGEESNEEAVAV
HHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MMDWTDRHCRFFHRQISRHTWLYTEMVTTGALLHGDVPRHLDFDEFEHPVALQLGGSEPD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCEEEEECCCCHH
DLAQAARLGQQWGYKEINLNCGCPSERVQRGAFGACLMAEPKLVAQGVTAMRDAVSIPVT
HHHHHHHHHHHCCCEEEEECCCCCHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHCCCEE
VKHRIGIDAIETYDFVRDFVGTIAEAGCETFIVHARNAILKGLSPKENREIPPLRYEVVY
EECCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
QLKRDFPHLEILLNGGVQTLEQIDTHLAHVDGVMIGREAYHHPHLMAAFDARYYGAGTEA
HHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHEECHHHHCCCCHHHHHCCHHCCCCCCC
PTRAEVEDAMIGYVGRWVERGGYAGAAVRHMLGLYRGVAGARGWRRVLSDSKALGRVKTL
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
ADAHALFDEARAHLRRGEESNEEAVAV
HHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]