| Definition | Ralstonia pickettii 12J chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_010682 |
| Length | 3,942,557 |
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The map label for this gene is glnA [H]
Identifier: 187928179
GI number: 187928179
Start: 1157212
End: 1158627
Strand: Direct
Name: glnA [H]
Synonym: Rpic_1086
Alternate gene names: 187928179
Gene position: 1157212-1158627 (Clockwise)
Preceding gene: 187928177
Following gene: 187928180
Centisome position: 29.35
GC content: 59.89
Gene sequence:
>1416_bases ATGTCCCAAAGCATTGCAGACGTGATGAAGCTCGTGAAGGAAAACGACGTCAAGTTCGTCGATTTCCGCTTCACCGATAC CAAGGGTAAGGAGCAGCACGTCTCGGTCCCCGTTTCGCATTTCGACGAAGACAAGTTCGAAAGCGGTCACGCATTCGACG GCTCGTCGATCGCCGGCTGGAAAGGCATCGAAGCGTCGGACATGCTGCTGGTGCCGGATGCAAATACCGCGCACATCGAC CCGTTTTACGAAGAGCCGACCCTGGTCCTGACCTGCGACGTGGTCGAGCCGTCGGACGGCAAGGGCTACGACCGCGACCC GCGCTCGATCGCCAAGCGCGCTGAAGCCTACCTGAAGAGCACCGGCCTGGGCGACACCGCCTACTTTGGTCCGGAACCCG AATTCTTCATCTTCGACGGCGTGACCTGGAACATCGACATGCAGGGTTGCTTCGTGAAGATCAATTCCGAAGAAGCTCCG TGGTCGTCGGGCAAGGAGTTCGAACACGGCAACTCGGGCCACCGTCCGGGCAAGAAGGGCGGCTACTTCCCGGTTGCCCC GATCGACACGTTCCAAGACATGCGCTCGGAAATGTGCCTGATTCTGGAATCGCTGGGTATTCCCGTTGAAGTGCACCACC ACGAAGTGGCAGGCCAAGGTCAGAACGAAATCGGCACGAAGTTCAGCACGCTGGTGCAACGCGCCGACTGGACCCAGCTG CAAAAGTACGTGATCCAGAACGTCGCGCACACCTATGGCAAGACGGCGACGTTCATGCCGAAGCCGGTCGTGGGCGACAA CGGTTCGGGCATGCATGTTCACCAATCGGTGTGGAAAGATGGCCAGAACCTGTTCGCAGGCAATGGTTACGCTGGCCTGT CGGAATTCGCGCTGTATTACATCGGCGGCATCATCAAGCACGCCCGCGCGCTGAATGCCCTCACCAACCCGGGTACGAAC TCGTACAAGCGTCTGGTGCCGGGCTTCGAGGCTCCGGTCAAGCTGGCTTACTCGGCACGTAACCGTTCGGCTTCGATCCG CATTCCGTACGTCGCCAACCCGAAGGGCCGTCGTATCGAGACCCGCTTCCCGGATCCGCTGATGAACCCGTACCTGGGCT TCTCGGCGCTGCTGATGGCCGGTCTGGATGGCGTGCAGAACAAGATCCACCCGGGCGAAGCTGCCGACAAGAACCTGTAC GACCTGCCGCCGGAAGAAGACGCAAAGATCCCGACCGTGTGCTCGAGCCTGGATCAGGCCCTCGAGTACCTGGACAAGGA TCGCGAGTTCCTGACCCGCGGTGGTGTGTTCTCCAACGCCATGCTCGACGCCTACATCGAACTGAAGATGGAAGAAGTCA CGCGCTTCCGCATGACGACGCACCCGCTCGAGTTCGAGATGTACTACTCGCTGTAA
Upstream 100 bases:
>100_bases AGATGTTTCGGAGTGAGGGCGGGTTGCGGCCGATTGCGCAGCGATCCGTCACACAACCGGCTCACAATACTTTGCGTTAT TGCGCTACCAGGAGAATGGC
Downstream 100 bases:
>100_bases TGTGCGAGCGCTCCGACCGATGTGTCGGGGCGAAGAGACGGTTGTGAGAGGAGGGGCGGCGCAGGTCGCCCTTCCTTTTT ATGAAGCGGAGTTTTGTGGC
Product: glutamine synthetase
Products: NA
Alternate protein names: Glutamate--ammonia ligase [H]
Number of amino acids: Translated: 471; Mature: 470
Protein sequence:
>471_residues MSQSIADVMKLVKENDVKFVDFRFTDTKGKEQHVSVPVSHFDEDKFESGHAFDGSSIAGWKGIEASDMLLVPDANTAHID PFYEEPTLVLTCDVVEPSDGKGYDRDPRSIAKRAEAYLKSTGLGDTAYFGPEPEFFIFDGVTWNIDMQGCFVKINSEEAP WSSGKEFEHGNSGHRPGKKGGYFPVAPIDTFQDMRSEMCLILESLGIPVEVHHHEVAGQGQNEIGTKFSTLVQRADWTQL QKYVIQNVAHTYGKTATFMPKPVVGDNGSGMHVHQSVWKDGQNLFAGNGYAGLSEFALYYIGGIIKHARALNALTNPGTN SYKRLVPGFEAPVKLAYSARNRSASIRIPYVANPKGRRIETRFPDPLMNPYLGFSALLMAGLDGVQNKIHPGEAADKNLY DLPPEEDAKIPTVCSSLDQALEYLDKDREFLTRGGVFSNAMLDAYIELKMEEVTRFRMTTHPLEFEMYYSL
Sequences:
>Translated_471_residues MSQSIADVMKLVKENDVKFVDFRFTDTKGKEQHVSVPVSHFDEDKFESGHAFDGSSIAGWKGIEASDMLLVPDANTAHID PFYEEPTLVLTCDVVEPSDGKGYDRDPRSIAKRAEAYLKSTGLGDTAYFGPEPEFFIFDGVTWNIDMQGCFVKINSEEAP WSSGKEFEHGNSGHRPGKKGGYFPVAPIDTFQDMRSEMCLILESLGIPVEVHHHEVAGQGQNEIGTKFSTLVQRADWTQL QKYVIQNVAHTYGKTATFMPKPVVGDNGSGMHVHQSVWKDGQNLFAGNGYAGLSEFALYYIGGIIKHARALNALTNPGTN SYKRLVPGFEAPVKLAYSARNRSASIRIPYVANPKGRRIETRFPDPLMNPYLGFSALLMAGLDGVQNKIHPGEAADKNLY DLPPEEDAKIPTVCSSLDQALEYLDKDREFLTRGGVFSNAMLDAYIELKMEEVTRFRMTTHPLEFEMYYSL >Mature_470_residues SQSIADVMKLVKENDVKFVDFRFTDTKGKEQHVSVPVSHFDEDKFESGHAFDGSSIAGWKGIEASDMLLVPDANTAHIDP FYEEPTLVLTCDVVEPSDGKGYDRDPRSIAKRAEAYLKSTGLGDTAYFGPEPEFFIFDGVTWNIDMQGCFVKINSEEAPW SSGKEFEHGNSGHRPGKKGGYFPVAPIDTFQDMRSEMCLILESLGIPVEVHHHEVAGQGQNEIGTKFSTLVQRADWTQLQ KYVIQNVAHTYGKTATFMPKPVVGDNGSGMHVHQSVWKDGQNLFAGNGYAGLSEFALYYIGGIIKHARALNALTNPGTNS YKRLVPGFEAPVKLAYSARNRSASIRIPYVANPKGRRIETRFPDPLMNPYLGFSALLMAGLDGVQNKIHPGEAADKNLYD LPPEEDAKIPTVCSSLDQALEYLDKDREFLTRGGVFSNAMLDAYIELKMEEVTRFRMTTHPLEFEMYYSL
Specific function: Unknown
COG id: COG0174
COG function: function code E; Glutamine synthetase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glutamine synthetase family [H]
Homologues:
Organism=Escherichia coli, GI1790301, Length=468, Percent_Identity=64.7435897435898, Blast_Score=658, Evalue=0.0, Organism=Escherichia coli, GI87081870, Length=400, Percent_Identity=25.5, Blast_Score=90, Evalue=3e-19,
Paralogues:
None
Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR008147 - InterPro: IPR014746 - InterPro: IPR008146 - InterPro: IPR004809 - InterPro: IPR001637 [H]
Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]
EC number: =6.3.1.2 [H]
Molecular weight: Translated: 52424; Mature: 52292
Theoretical pI: Translated: 5.33; Mature: 5.33
Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 3.0 %Met (Translated Protein) 3.8 %Cys+Met (Translated Protein) 0.9 %Cys (Mature Protein) 2.8 %Met (Mature Protein) 3.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSQSIADVMKLVKENDVKFVDFRFTDTKGKEQHVSVPVSHFDEDKFESGHAFDGSSIAGW CCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCHHHCCCHHCCCCCCCCCCCCCCC KGIEASDMLLVPDANTAHIDPFYEEPTLVLTCDVVEPSDGKGYDRDPRSIAKRAEAYLKS CCCCCCCEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH TGLGDTAYFGPEPEFFIFDGVTWNIDMQGCFVKINSEEAPWSSGKEFEHGNSGHRPGKKG CCCCCCCCCCCCCCEEEECCEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC GYFPVAPIDTFQDMRSEMCLILESLGIPVEVHHHEVAGQGQNEIGTKFSTLVQRADWTQL CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHH QKYVIQNVAHTYGKTATFMPKPVVGDNGSGMHVHQSVWKDGQNLFAGNGYAGLSEFALYY HHHHHHHHHHHHCCCEECCCCCCCCCCCCCCEEHHHHHHCCCCEECCCCCCCHHHHHHHH IGGIIKHARALNALTNPGTNSYKRLVPGFEAPVKLAYSARNRSASIRIPYVANPKGRRIE HHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCEEE TRFPDPLMNPYLGFSALLMAGLDGVQNKIHPGEAADKNLYDLPPEEDAKIPTVCSSLDQA CCCCCHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH LEYLDKDREFLTRGGVFSNAMLDAYIELKMEEVTRFRMTTHPLEFEMYYSL HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC >Mature Secondary Structure SQSIADVMKLVKENDVKFVDFRFTDTKGKEQHVSVPVSHFDEDKFESGHAFDGSSIAGW CCHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCHHHCCCHHCCCCCCCCCCCCCCC KGIEASDMLLVPDANTAHIDPFYEEPTLVLTCDVVEPSDGKGYDRDPRSIAKRAEAYLKS CCCCCCCEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH TGLGDTAYFGPEPEFFIFDGVTWNIDMQGCFVKINSEEAPWSSGKEFEHGNSGHRPGKKG CCCCCCCCCCCCCCEEEECCEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC GYFPVAPIDTFQDMRSEMCLILESLGIPVEVHHHEVAGQGQNEIGTKFSTLVQRADWTQL CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHH QKYVIQNVAHTYGKTATFMPKPVVGDNGSGMHVHQSVWKDGQNLFAGNGYAGLSEFALYY HHHHHHHHHHHHCCCEECCCCCCCCCCCCCCEEHHHHHHCCCCEECCCCCCCHHHHHHHH IGGIIKHARALNALTNPGTNSYKRLVPGFEAPVKLAYSARNRSASIRIPYVANPKGRRIE HHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCEEE TRFPDPLMNPYLGFSALLMAGLDGVQNKIHPGEAADKNLYDLPPEEDAKIPTVCSSLDQA CCCCCHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH LEYLDKDREFLTRGGVFSNAMLDAYIELKMEEVTRFRMTTHPLEFEMYYSL HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 1969923 [H]