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The map label for this gene is purA

Identifier: 187928163

GI number: 187928163

Start: 1137042

End: 1138382

Strand: Direct

Name: purA

Synonym: Rpic_1070

Alternate gene names: 187928163

Gene position: 1137042-1138382 (Clockwise)

Preceding gene: 187928162

Following gene: 187928164

Centisome position: 28.84

GC content: 62.19

Gene sequence:

>1341_bases
ATGTCCGCACCAGCAGTGAGCCAAGGACGCAATGTCGTCGTCATCGGCACCCAATGGGGTGACGAAGGTAAAGGGAAAAT
CGTCGATTGGCTGACCGATCATGCGCAAGGCGTGGTTCGTTTCCAGGGCGGACATAACGCAGGCCATACCCTCATCATCG
GCGGTAAGAAGACGATTCTGCGTCTGATCCCGTCGGGCATCATGCGTGACGGCGTCGCGTGTTACATCGGCAACGGTGTT
GTGCTGTCGCCTGAGGCGCTGTTCAAGGAAATCGACGAGCTTGAATCCGCCGGTGTGCAGGTGCAGAACCGACTGCGCAT
TTCGGAAGCGACCAACCTGATCCTGCCGTACCACGTGGCCATCGACAAGGCGCGCGAAGCCAAGCGCGGCGCCGCCAAGA
TCGGCACCACCGGTCGCGGTATCGGCCCGGCGTACGAAGACAAGGTGGCCCGCCGCGGGCTGCGCGTGCAGGACCTGTTC
GACCCGGCTTACTTTGCCGAGCGTCTGCGCGAGAACCTGGATTTCCACAACTTTGTTCTGACGCAGTACCTGAATCATCC
GGCGCTGGACTTCCAGCAAACGCTGGACGAGATGCTGTCCTACGCCGGCCGCCTCGCCCCGATGGTGACGGACGTGTCGG
CTGAACTGTTCGCTGCCAACGCCGCCGGTAAGAACCTGATGTTCGAGGGCGCGCAAGGTACGCTGCTCGATATCGACCAC
GGCACCTATCCGTTCGTCACGTCGAGCAACTGCGTGGCGGGTAATGCCGCCGCGGGCGCGGGTGTCGGCCCGGGCCAACT
GCATTACATCCTTGGCATCACCAAGGCGTATTGCACGCGCGTGGGTTCCGGCCCGTTCCCGAGCGAACTGTATGACGCGG
ACAACCCCGCGCGCCAAGATCCGATTGGCGTGCGTCTGGCCAATGTCGGCAAGGAATTCGGCTCGGTCACCGGCCGGCCG
CGCCGCACGGGCTGGTTGGATGCTGCCGCGCTGCGCCGAGCGATCCAGATCAACGGTGTGTCGGGCCTGTGCATGACCAA
GCTCGATGTGCTGGATGGCCTGGAAACCCTCAAGCTGTGCGTTGGCTACATGCTCGATGGCAAGCAAATTGACATCTTGC
CGCGTGGCTCCGATGCCGTGGCGCGTTGCCAGCCGATCTACGAAGAATTCCCGGGTTGGAACACCTCGACCTTCGGTTTG
AAGGAGTGGGATGCGCTGCCCCAGACCGCTCAGGCTTACCTGAAGCGCGTCGAAGAAGTGGCCGGTATTCCGATTGCCAT
GATTTCGACCGGTCCTGACCGGGACGAGACCATCCTGCTGCGTCATCCCTACAAGGACTGA

Upstream 100 bases:

>100_bases
TCTGAGGCTGTTGCATAACGTTGTGTAAATACCCCGTAATTTCAGGGAAGCCCCTGGCAACAAGGGTAGAATACGTTTTT
AACCCATCTGCAAATTCAAC

Downstream 100 bases:

>100_bases
CGCGCAGCTCCCAGATTCGCCCGGTGATTTGCCGGGCGAATTTGCATGGAGCCCGTTTTCAACAGAACACGATAGCGGCA
ACTCACGCCGTATTGACGAT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MSAPAVSQGRNVVVIGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKTILRLIPSGIMRDGVACYIGNGV
VLSPEALFKEIDELESAGVQVQNRLRISEATNLILPYHVAIDKAREAKRGAAKIGTTGRGIGPAYEDKVARRGLRVQDLF
DPAYFAERLRENLDFHNFVLTQYLNHPALDFQQTLDEMLSYAGRLAPMVTDVSAELFAANAAGKNLMFEGAQGTLLDIDH
GTYPFVTSSNCVAGNAAAGAGVGPGQLHYILGITKAYCTRVGSGPFPSELYDADNPARQDPIGVRLANVGKEFGSVTGRP
RRTGWLDAAALRRAIQINGVSGLCMTKLDVLDGLETLKLCVGYMLDGKQIDILPRGSDAVARCQPIYEEFPGWNTSTFGL
KEWDALPQTAQAYLKRVEEVAGIPIAMISTGPDRDETILLRHPYKD

Sequences:

>Translated_446_residues
MSAPAVSQGRNVVVIGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKTILRLIPSGIMRDGVACYIGNGV
VLSPEALFKEIDELESAGVQVQNRLRISEATNLILPYHVAIDKAREAKRGAAKIGTTGRGIGPAYEDKVARRGLRVQDLF
DPAYFAERLRENLDFHNFVLTQYLNHPALDFQQTLDEMLSYAGRLAPMVTDVSAELFAANAAGKNLMFEGAQGTLLDIDH
GTYPFVTSSNCVAGNAAAGAGVGPGQLHYILGITKAYCTRVGSGPFPSELYDADNPARQDPIGVRLANVGKEFGSVTGRP
RRTGWLDAAALRRAIQINGVSGLCMTKLDVLDGLETLKLCVGYMLDGKQIDILPRGSDAVARCQPIYEEFPGWNTSTFGL
KEWDALPQTAQAYLKRVEEVAGIPIAMISTGPDRDETILLRHPYKD
>Mature_445_residues
SAPAVSQGRNVVVIGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKTILRLIPSGIMRDGVACYIGNGVV
LSPEALFKEIDELESAGVQVQNRLRISEATNLILPYHVAIDKAREAKRGAAKIGTTGRGIGPAYEDKVARRGLRVQDLFD
PAYFAERLRENLDFHNFVLTQYLNHPALDFQQTLDEMLSYAGRLAPMVTDVSAELFAANAAGKNLMFEGAQGTLLDIDHG
TYPFVTSSNCVAGNAAAGAGVGPGQLHYILGITKAYCTRVGSGPFPSELYDADNPARQDPIGVRLANVGKEFGSVTGRPR
RTGWLDAAALRRAIQINGVSGLCMTKLDVLDGLETLKLCVGYMLDGKQIDILPRGSDAVARCQPIYEEFPGWNTSTFGLK
EWDALPQTAQAYLKRVEEVAGIPIAMISTGPDRDETILLRHPYKD

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=444, Percent_Identity=40.7657657657658, Blast_Score=318, Evalue=6e-87,
Organism=Homo sapiens, GI34577063, Length=442, Percent_Identity=39.8190045248869, Blast_Score=303, Evalue=3e-82,
Organism=Homo sapiens, GI40316944, Length=405, Percent_Identity=39.0123456790123, Blast_Score=281, Evalue=1e-75,
Organism=Escherichia coli, GI1790620, Length=437, Percent_Identity=60.6407322654462, Blast_Score=538, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI25146555, Length=436, Percent_Identity=39.9082568807339, Blast_Score=313, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI25146553, Length=439, Percent_Identity=39.8633257403189, Blast_Score=313, Evalue=1e-85,
Organism=Saccharomyces cerevisiae, GI6324109, Length=437, Percent_Identity=37.5286041189931, Blast_Score=307, Evalue=2e-84,
Organism=Drosophila melanogaster, GI21356033, Length=431, Percent_Identity=38.9791183294664, Blast_Score=306, Evalue=2e-83,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_RALPJ (B2U9W0)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001898650.1
- ProteinModelPortal:   B2U9W0
- SMR:   B2U9W0
- GeneID:   6289436
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_1070
- HOGENOM:   HBG658237
- OMA:   YVLGIIK
- ProtClustDB:   PRK01117
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 48233; Mature: 48101

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 21-21 ACT_SITE 49-49 BINDING 137-137 BINDING 151-151 BINDING 232-232 BINDING 247-247 BINDING 319-319 BINDING 321-321

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPAVSQGRNVVVIGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKTIL
CCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCEEEECCHHHHH
RLIPSGIMRDGVACYIGNGVVLSPEALFKEIDELESAGVQVQNRLRISEATNLILPYHVA
HHHHHHHHHCCEEEEECCCEEECHHHHHHHHHHHHHCCCCHHHHEEHHHHCCEEEEEHHH
IDKAREAKRGAAKIGTTGRGIGPAYEDKVARRGLRVQDLFDPAYFAERLRENLDFHNFVL
HHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCCHHHHHH
TQYLNHPALDFQQTLDEMLSYAGRLAPMVTDVSAELFAANAAGKNLMFEGAQGTLLDIDH
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHEECCCCCCEEEECCCCCEEECCC
GTYPFVTSSNCVAGNAAAGAGVGPGQLHYILGITKAYCTRVGSGPFPSELYDADNPARQD
CCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCC
PIGVRLANVGKEFGSVTGRPRRTGWLDAAALRRAIQINGVSGLCMTKLDVLDGLETLKLC
CCCEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHH
VGYMLDGKQIDILPRGSDAVARCQPIYEEFPGWNTSTFGLKEWDALPQTAQAYLKRVEEV
HHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHH
AGIPIAMISTGPDRDETILLRHPYKD
CCCCEEEEECCCCCCCEEEEECCCCC
>Mature Secondary Structure 
SAPAVSQGRNVVVIGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTLIIGGKKTIL
CCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCEEEECCHHHHH
RLIPSGIMRDGVACYIGNGVVLSPEALFKEIDELESAGVQVQNRLRISEATNLILPYHVA
HHHHHHHHHCCEEEEECCCEEECHHHHHHHHHHHHHCCCCHHHHEEHHHHCCEEEEEHHH
IDKAREAKRGAAKIGTTGRGIGPAYEDKVARRGLRVQDLFDPAYFAERLRENLDFHNFVL
HHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCCHHHHHH
TQYLNHPALDFQQTLDEMLSYAGRLAPMVTDVSAELFAANAAGKNLMFEGAQGTLLDIDH
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHEECCCCCCEEEECCCCCEEECCC
GTYPFVTSSNCVAGNAAAGAGVGPGQLHYILGITKAYCTRVGSGPFPSELYDADNPARQD
CCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCC
PIGVRLANVGKEFGSVTGRPRRTGWLDAAALRRAIQINGVSGLCMTKLDVLDGLETLKLC
CCCEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHH
VGYMLDGKQIDILPRGSDAVARCQPIYEEFPGWNTSTFGLKEWDALPQTAQAYLKRVEEV
HHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHH
AGIPIAMISTGPDRDETILLRHPYKD
CCCCEEEEECCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA