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The map label for this gene is ispG [H]

Identifier: 187928152

GI number: 187928152

Start: 1125040

End: 1126326

Strand: Direct

Name: ispG [H]

Synonym: Rpic_1059

Alternate gene names: 187928152

Gene position: 1125040-1126326 (Clockwise)

Preceding gene: 187928151

Following gene: 187928153

Centisome position: 28.54

GC content: 64.34

Gene sequence:

>1287_bases
ATGGAAATCTCCACGCCTCTCGATTGCGCTCCAGCCTTGGTGGGCCCGCTGCCGCGCCGGAACTCGCGCGCCGCGCAAAT
CCGCTGGGGTGATCGTGTCGTGCACGTCGGTGGCGACGCGCCGGTCTGCGTGCAATCGATGACGAATACCGACACTTCGG
ACGCCATCGGTACCGCAATCCAGGTAAAGGAGCTGGCGCGTGCCGGCTCTGAGTTGGTGCGCATCACAGTGGATACCCCG
GCCGCCGCGGCCGCGGTGCCTGCCATCCGCGAGCAGCTCGACCGCATGGGTGTGGATGTGCCGCTTGTCGGCGATTTCCA
CTACAACGGCCACAAGCTGCTGCAGGACTATCCGGAGTGCGCGCAGGCGCTATCCAAGTACCGGATCAACCCCGGCAACG
TCGGGCAGGGTGCCAAGCGCGACACGCAGTTCGCGCAGATGATCGAGATTGCTTGCCGCTATGACAAGCCGGTGCGTATC
GGCGTGAACTGGGGCAGCCTCGATCAGGATCTGCTTGCGCGCATCATGGACGAGAACGCGCAACGTGCAACCCCCTGGGA
TGCCCGCGCCGTGATGGTCGAGGCGCTGATCACCTCCGCGATCCAGTCCGCGCAAAAGGCTGAAGAACTGGGCTTGCCAG
GCAGCCAGATCATCCTGTCGTGCAAGGTGTCGGCGGTGCAGGATCTGATTGCTGTGTACCGCGAGTTGGCGCGTCGCTGC
CATTACGCGCTGCACCTGGGCCTGACCGAGGCCGGCATGGGGAGCAAGGGTATCGTCGCTTCCACGGCGGCGTTGTCGGT
GCTGCTGCAGGAAGGCATCGGCGACACGATCCGCATTTCGCTCACGCCGGAACCGGGTGCGCCGCGCGAGAAGGAAGTCA
TCGTCGCGCAAGAGATTCTGCAGACCATGGGCCTGCGCAATTTCACGCCCATGGTGATCGCGTGCCCGGGTTGCGGGCGC
ACGACCAGCACGGTGTTCCAGGAACTGGCGGCGCGCATCCAGTCGTATTTGCGCGAGCAGATGCCGGTGTGGAAGGAGCA
GTACCCCGGCGTCGAAGAGATGAATGTGGCCGTGATGGGCTGCATCGTCAACGGCCCCGGAGAGAGCAAGCACGCCAATA
TCGGCATTTCGCTGCCAGGCACGGGCGAGTCGCCGGCGGCGCCGGTTTTCGTTGACGGCGTGAAGGTGAAAACACTGCGC
GGCGAGCGCATCACAGAAGAATTCCAGGCGATCGTCGACGAGTACGTGCGGACGCATTACGGCGCACAGACTCCTGCGAC
GGTCTAA

Upstream 100 bases:

>100_bases
ATCTGCAGAAGTACGCCCGCAATCGCGTGGCACGGGCCACGTTGCCCTGAACCGTTTTGTAATTGGCGCGGTGCCTGTAG
GCAGCGCGCCCTGTCTGATC

Downstream 100 bases:

>100_bases
AGCGACATCAGGCTTCGGAAACCAAAGCCGAAGCACGAAACGACCGGCCGGCTCTGCATGAAGGTAGATGCGAGAAGTCG
GCTTTCAACGTGACATCATG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MEISTPLDCAPALVGPLPRRNSRAAQIRWGDRVVHVGGDAPVCVQSMTNTDTSDAIGTAIQVKELARAGSELVRITVDTP
AAAAAVPAIREQLDRMGVDVPLVGDFHYNGHKLLQDYPECAQALSKYRINPGNVGQGAKRDTQFAQMIEIACRYDKPVRI
GVNWGSLDQDLLARIMDENAQRATPWDARAVMVEALITSAIQSAQKAEELGLPGSQIILSCKVSAVQDLIAVYRELARRC
HYALHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGAPREKEVIVAQEILQTMGLRNFTPMVIACPGCGR
TTSTVFQELAARIQSYLREQMPVWKEQYPGVEEMNVAVMGCIVNGPGESKHANIGISLPGTGESPAAPVFVDGVKVKTLR
GERITEEFQAIVDEYVRTHYGAQTPATV

Sequences:

>Translated_428_residues
MEISTPLDCAPALVGPLPRRNSRAAQIRWGDRVVHVGGDAPVCVQSMTNTDTSDAIGTAIQVKELARAGSELVRITVDTP
AAAAAVPAIREQLDRMGVDVPLVGDFHYNGHKLLQDYPECAQALSKYRINPGNVGQGAKRDTQFAQMIEIACRYDKPVRI
GVNWGSLDQDLLARIMDENAQRATPWDARAVMVEALITSAIQSAQKAEELGLPGSQIILSCKVSAVQDLIAVYRELARRC
HYALHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGAPREKEVIVAQEILQTMGLRNFTPMVIACPGCGR
TTSTVFQELAARIQSYLREQMPVWKEQYPGVEEMNVAVMGCIVNGPGESKHANIGISLPGTGESPAAPVFVDGVKVKTLR
GERITEEFQAIVDEYVRTHYGAQTPATV
>Mature_428_residues
MEISTPLDCAPALVGPLPRRNSRAAQIRWGDRVVHVGGDAPVCVQSMTNTDTSDAIGTAIQVKELARAGSELVRITVDTP
AAAAAVPAIREQLDRMGVDVPLVGDFHYNGHKLLQDYPECAQALSKYRINPGNVGQGAKRDTQFAQMIEIACRYDKPVRI
GVNWGSLDQDLLARIMDENAQRATPWDARAVMVEALITSAIQSAQKAEELGLPGSQIILSCKVSAVQDLIAVYRELARRC
HYALHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGAPREKEVIVAQEILQTMGLRNFTPMVIACPGCGR
TTSTVFQELAARIQSYLREQMPVWKEQYPGVEEMNVAVMGCIVNGPGESKHANIGISLPGTGESPAAPVFVDGVKVKTLR
GERITEEFQAIVDEYVRTHYGAQTPATV

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=402, Percent_Identity=35.0746268656716, Blast_Score=187, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 46117; Mature: 46117

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEISTPLDCAPALVGPLPRRNSRAAQIRWGDRVVHVGGDAPVCVQSMTNTDTSDAIGTAI
CCCCCCCCCHHHHHCCCCCCCCCEEEEEECCEEEEECCCCCHHHHHHCCCCCHHHHHHHH
QVKELARAGSELVRITVDTPAAAAAVPAIREQLDRMGVDVPLVGDFHYNGHKLLQDYPEC
HHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHH
AQALSKYRINPGNVGQGAKRDTQFAQMIEIACRYDKPVRIGVNWGSLDQDLLARIMDENA
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHCCCC
QRATPWDARAVMVEALITSAIQSAQKAEELGLPGSQIILSCKVSAVQDLIAVYRELARRC
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHH
HYALHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGAPREKEVIVAQEIL
HHHHEECCHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHH
QTMGLRNFTPMVIACPGCGRTTSTVFQELAARIQSYLREQMPVWKEQYPGVEEMNVAVMG
HHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHCCEEEEE
CIVNGPGESKHANIGISLPGTGESPAAPVFVDGVKVKTLRGERITEEFQAIVDEYVRTHY
EEECCCCCCCCCEEEEECCCCCCCCCCCEEECCEEEEEECCHHHHHHHHHHHHHHHHHHC
GAQTPATV
CCCCCCCC
>Mature Secondary Structure
MEISTPLDCAPALVGPLPRRNSRAAQIRWGDRVVHVGGDAPVCVQSMTNTDTSDAIGTAI
CCCCCCCCCHHHHHCCCCCCCCCEEEEEECCEEEEECCCCCHHHHHHCCCCCHHHHHHHH
QVKELARAGSELVRITVDTPAAAAAVPAIREQLDRMGVDVPLVGDFHYNGHKLLQDYPEC
HHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHH
AQALSKYRINPGNVGQGAKRDTQFAQMIEIACRYDKPVRIGVNWGSLDQDLLARIMDENA
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHCCCC
QRATPWDARAVMVEALITSAIQSAQKAEELGLPGSQIILSCKVSAVQDLIAVYRELARRC
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHH
HYALHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGAPREKEVIVAQEIL
HHHHEECCHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHH
QTMGLRNFTPMVIACPGCGRTTSTVFQELAARIQSYLREQMPVWKEQYPGVEEMNVAVMG
HHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHCCEEEEE
CIVNGPGESKHANIGISLPGTGESPAAPVFVDGVKVKTLRGERITEEFQAIVDEYVRTHY
EEECCCCCCCCCEEEEECCCCCCCCCCCEEECCEEEEEECCHHHHHHHHHHHHHHHHHHC
GAQTPATV
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]