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The map label for this gene is nemR [H]

Identifier: 187928138

GI number: 187928138

Start: 1111105

End: 1111773

Strand: Direct

Name: nemR [H]

Synonym: Rpic_1045

Alternate gene names: 187928138

Gene position: 1111105-1111773 (Clockwise)

Preceding gene: 187928137

Following gene: 187928139

Centisome position: 28.18

GC content: 63.23

Gene sequence:

>669_bases
ATGCAGCTAGACGGTCGGTCTACTACAATGCGATCGGTCATGAGCAAGCACCCCGAACCCGTCACCGCTGAAGGCGCCGA
AGCCCGGCACGACACCCGCGACCGCATCCTGGACGCTGGCGCCGAGCTGATCCTCGGGCGCGGTTTTTCGGCCGTCGGTT
TGGCGGAAATCCTTGGCCGTGCGCAGGTACCCAAGGGCTCGTTTTATTACTACTTCGGGTCGAAGGAAGAATTTGGCGTC
GCCATGCTGGAGCGCTATTTCCAGGACTACGACGCGGGCGTTGTCAGCCTGTTCAACGACACGCGCATCACCGCCCGTGA
GCGGCTGCTGCGCTATTTCTTGGCCTGGATCGATCTGCATGAGCGCAGCGCGTGCGAGGTCACGTGCCTGGCGGTCAAGC
TTTCAGGCGAGGTATCGGATTTGTCGGAGCCGATGCGCAAGGTGTTGTCGACAGGGATGACGCGCATGGTCGAGCGCATT
GCCACCGCGATCGAGGCCGGTGTCGCCGACGGCTCGTTGGCGCCGGTGCAGGACGCGCACGAACTGGCCGAAGGCCTGTA
CGCCATGTGGATGGGTGGTGCACTGCTGGCCAAGGCGCACCGCACGGTCGACGCGTTCAAGAGCTGTGTCGCGCAAACGG
AAGTGCTGCTGGCCAAGCCCAAACACTGA

Upstream 100 bases:

>100_bases
AAAACGCCTGATGCGCTGGCAACCGAAGAGCGGCGAGACTGAAAAGCTCTGAGCGCGCTGACGACGCGAACGTCACATCG
ACCCTGCCAGGAGCAGGGTC

Downstream 100 bases:

>100_bases
TTCCATTCTGAAACCAATTGCCTGCGAGGCACGTTCCTCGCTTTTTCTTTGATTCCGGTTCTAGATGACCGGTCGAATAG
TCCCTCTTCCAGGAGCTGAT

Product: TetR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MQLDGRSTTMRSVMSKHPEPVTAEGAEARHDTRDRILDAGAELILGRGFSAVGLAEILGRAQVPKGSFYYYFGSKEEFGV
AMLERYFQDYDAGVVSLFNDTRITARERLLRYFLAWIDLHERSACEVTCLAVKLSGEVSDLSEPMRKVLSTGMTRMVERI
ATAIEAGVADGSLAPVQDAHELAEGLYAMWMGGALLAKAHRTVDAFKSCVAQTEVLLAKPKH

Sequences:

>Translated_222_residues
MQLDGRSTTMRSVMSKHPEPVTAEGAEARHDTRDRILDAGAELILGRGFSAVGLAEILGRAQVPKGSFYYYFGSKEEFGV
AMLERYFQDYDAGVVSLFNDTRITARERLLRYFLAWIDLHERSACEVTCLAVKLSGEVSDLSEPMRKVLSTGMTRMVERI
ATAIEAGVADGSLAPVQDAHELAEGLYAMWMGGALLAKAHRTVDAFKSCVAQTEVLLAKPKH
>Mature_222_residues
MQLDGRSTTMRSVMSKHPEPVTAEGAEARHDTRDRILDAGAELILGRGFSAVGLAEILGRAQVPKGSFYYYFGSKEEFGV
AMLERYFQDYDAGVVSLFNDTRITARERLLRYFLAWIDLHERSACEVTCLAVKLSGEVSDLSEPMRKVLSTGMTRMVERI
ATAIEAGVADGSLAPVQDAHELAEGLYAMWMGGALLAKAHRTVDAFKSCVAQTEVLLAKPKH

Specific function: Represses the transcription of the nemRA operon by binding to the nemR box [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693141, Length=207, Percent_Identity=37.6811594202899, Blast_Score=145, Evalue=2e-36,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR001647 [H]

Pfam domain/function: PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 24404; Mature: 24404

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLDGRSTTMRSVMSKHPEPVTAEGAEARHDTRDRILDAGAELILGRGFSAVGLAEILGR
CCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
AQVPKGSFYYYFGSKEEFGVAMLERYFQDYDAGVVSLFNDTRITARERLLRYFLAWIDLH
CCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ERSACEVTCLAVKLSGEVSDLSEPMRKVLSTGMTRMVERIATAIEAGVADGSLAPVQDAH
CCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
ELAEGLYAMWMGGALLAKAHRTVDAFKSCVAQTEVLLAKPKH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQLDGRSTTMRSVMSKHPEPVTAEGAEARHDTRDRILDAGAELILGRGFSAVGLAEILGR
CCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
AQVPKGSFYYYFGSKEEFGVAMLERYFQDYDAGVVSLFNDTRITARERLLRYFLAWIDLH
CCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ERSACEVTCLAVKLSGEVSDLSEPMRKVLSTGMTRMVERIATAIEAGVADGSLAPVQDAH
CCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
ELAEGLYAMWMGGALLAKAHRTVDAFKSCVAQTEVLLAKPKH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]