Click here to switch to the map view.

The map label for this gene is tilS [H]

Identifier: 187928104

GI number: 187928104

Start: 1067968

End: 1069386

Strand: Direct

Name: tilS [H]

Synonym: Rpic_1011

Alternate gene names: 187928104

Gene position: 1067968-1069386 (Clockwise)

Preceding gene: 187928103

Following gene: 187928105

Centisome position: 27.09

GC content: 66.17

Gene sequence:

>1419_bases
ATGGCAAGTTCAAGGAAACCGAAGGTCGCTGATTCGTCGGCTGATCTCGTCAACAAGGTCGCACAACGCGTTGCCGCGTG
TGCGGCCTTTGTTGTTTCTGGCGAGATCGAAACACCGACCGTGGCGATTGCACTGTCGGGCGGGCGGGATTCCGCCGCGC
TCCTGCACGCGTGTGCTGCGTGGCGGGATGCGGGCGCCCGTGTGCAGCTCGTTGCCCTGCATATTCACCACGGCTTGCAG
GCCGATGCCGATGTCTGGGAATGTGCCTGTCAACGTATGGCCCAAGCCGCTGGCGTGGCGTTTCATGCGCGTCGCGTCTA
CGTAGAGATTGATGCGGGGCAGGGCGTTGAAGAAGCGGCGCGCGAAGCCCGGTATGCAGCGCTCGAAGCGCTGTGCGCGC
AGACAGGCGCCTCCATTCTGCTGACGGCCCATCATCAGGATGACCAGGCTGAGACGGTACTGCTGCAATTGATGCGCGGC
GCCGGCCTCGATGGCCTCGCCGCCATGCCGATGGCCCGGGCGGGTGCAGTCACGTTGCTGCGTCCATGGGTCGATGCACC
GCGCAGCGAGATCGAGCTGTACGCACACGCCCACGCGCTCTGCTGGGTCGAAGACCCGTCCAACACCGACGCACGCTACG
CACGCAATGCATTGCGTCCACTGCTCGCAGGCATGGCGGACCATTTCCCGGCCTATCGCGAGGCGCTGTCGCGCAGCGCG
GCACATCTGGCGGATGCGGCCGCATTGATCGACGAGATCGCTCAGGCGGATTTGACGCGCATTGCTTCGGCGAATGGACT
GGATGTAGCGGCGCTGATTGCGCTGTCCGCGCCACGTCAGCGTGCGGTGCTGCGTGCATGGTTGACCAAAGCGGGCATGC
GCGCGCTGTCGAGTCGCCGCCTGGAGGATCTGCATACGCAACTGATTGACGCACGAGATGACGGTGCACTGCGCATTGAA
TTGCCGACTGGGCAGGTCAGGCGCTACCGTGGCGTTGCATGGATCGACACTGGCGCCAACGATCGCCCCGGGCAGGCCGC
GGTCGCCATCGGGGCCCAGCTGTTCGAGCCGGCGCATCCGGCCGAGCAGCGTGTGACGGTGGACGCATGGGGCGGGGCGC
TGTTGTTTGCGCCCGTGGCGAGCGATGGCGTTGCGACGCAAACTTTGCAAGCGCCGCTGGTCCTGTCGCCCCGGCGCGGC
GGCGAGCGCATCGCGCTGCGCCCGGGCGGGCCGTCGCGGGCGCTCAAGCAGGCTTACCAGGAAGCCGGCATTCCTGCGTG
GGAGCGCCAGCGTTTGCCACTGCTCTATGCAGGCGAAATGCTAGTGTTTGCCGCGGGGCTGGGAATGAACCAGGCGGCCA
CGCATAGCGGTACTGGCTGGCGCATCACGTGGCGTCCTGGCCTTGAGGGTGCGTCGTGA

Upstream 100 bases:

>100_bases
GCGACGATGCTCAAGCGCGCGCTGGCCGAATCGCTGCGCCAGTTCCAGGGCATGAAGACGTCGGAACTCCAGGCGCGCCG
TCACGAGCGCCTGATGGCTT

Downstream 100 bases:

>100_bases
TACGCATCGTCACCGGCGGATTGCTTGCCGATCGATGCAAATTGCTGTATTTTCAAAAGCTTTTGCACCGCTCCGCCTCC
TCCTGATCTTTCCAAGATGG

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MASSRKPKVADSSADLVNKVAQRVAACAAFVVSGEIETPTVAIALSGGRDSAALLHACAAWRDAGARVQLVALHIHHGLQ
ADADVWECACQRMAQAAGVAFHARRVYVEIDAGQGVEEAAREARYAALEALCAQTGASILLTAHHQDDQAETVLLQLMRG
AGLDGLAAMPMARAGAVTLLRPWVDAPRSEIELYAHAHALCWVEDPSNTDARYARNALRPLLAGMADHFPAYREALSRSA
AHLADAAALIDEIAQADLTRIASANGLDVAALIALSAPRQRAVLRAWLTKAGMRALSSRRLEDLHTQLIDARDDGALRIE
LPTGQVRRYRGVAWIDTGANDRPGQAAVAIGAQLFEPAHPAEQRVTVDAWGGALLFAPVASDGVATQTLQAPLVLSPRRG
GERIALRPGGPSRALKQAYQEAGIPAWERQRLPLLYAGEMLVFAAGLGMNQAATHSGTGWRITWRPGLEGAS

Sequences:

>Translated_472_residues
MASSRKPKVADSSADLVNKVAQRVAACAAFVVSGEIETPTVAIALSGGRDSAALLHACAAWRDAGARVQLVALHIHHGLQ
ADADVWECACQRMAQAAGVAFHARRVYVEIDAGQGVEEAAREARYAALEALCAQTGASILLTAHHQDDQAETVLLQLMRG
AGLDGLAAMPMARAGAVTLLRPWVDAPRSEIELYAHAHALCWVEDPSNTDARYARNALRPLLAGMADHFPAYREALSRSA
AHLADAAALIDEIAQADLTRIASANGLDVAALIALSAPRQRAVLRAWLTKAGMRALSSRRLEDLHTQLIDARDDGALRIE
LPTGQVRRYRGVAWIDTGANDRPGQAAVAIGAQLFEPAHPAEQRVTVDAWGGALLFAPVASDGVATQTLQAPLVLSPRRG
GERIALRPGGPSRALKQAYQEAGIPAWERQRLPLLYAGEMLVFAAGLGMNQAATHSGTGWRITWRPGLEGAS
>Mature_471_residues
ASSRKPKVADSSADLVNKVAQRVAACAAFVVSGEIETPTVAIALSGGRDSAALLHACAAWRDAGARVQLVALHIHHGLQA
DADVWECACQRMAQAAGVAFHARRVYVEIDAGQGVEEAAREARYAALEALCAQTGASILLTAHHQDDQAETVLLQLMRGA
GLDGLAAMPMARAGAVTLLRPWVDAPRSEIELYAHAHALCWVEDPSNTDARYARNALRPLLAGMADHFPAYREALSRSAA
HLADAAALIDEIAQADLTRIASANGLDVAALIALSAPRQRAVLRAWLTKAGMRALSSRRLEDLHTQLIDARDDGALRIEL
PTGQVRRYRGVAWIDTGANDRPGQAAVAIGAQLFEPAHPAEQRVTVDAWGGALLFAPVASDGVATQTLQAPLVLSPRRGG
ERIALRPGGPSRALKQAYQEAGIPAWERQRLPLLYAGEMLVFAAGLGMNQAATHSGTGWRITWRPGLEGAS

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=438, Percent_Identity=34.4748858447489, Blast_Score=183, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 50101; Mature: 49970

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSRKPKVADSSADLVNKVAQRVAACAAFVVSGEIETPTVAIALSGGRDSAALLHACAA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHH
WRDAGARVQLVALHIHHGLQADADVWECACQRMAQAAGVAFHARRVYVEIDAGQGVEEAA
HHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEEEEEECCCCHHHHH
REARYAALEALCAQTGASILLTAHHQDDQAETVLLQLMRGAGLDGLAAMPMARAGAVTLL
HHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHCCHHHCCHHHEE
RPWVDAPRSEIELYAHAHALCWVEDPSNTDARYARNALRPLLAGMADHFPAYREALSRSA
HHHHCCCHHHHHHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
AHLADAAALIDEIAQADLTRIASANGLDVAALIALSAPRQRAVLRAWLTKAGMRALSSRR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LEDLHTQLIDARDDGALRIELPTGQVRRYRGVAWIDTGANDRPGQAAVAIGAQLFEPAHP
HHHHHHHHHCCCCCCEEEEECCCCHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHCCCCC
AEQRVTVDAWGGALLFAPVASDGVATQTLQAPLVLSPRRGGERIALRPGGPSRALKQAYQ
HHHEEEEEECCCEEEEEECCCCCCCHHHHCCCEEECCCCCCCEEEECCCCHHHHHHHHHH
EAGIPAWERQRLPLLYAGEMLVFAAGLGMNQAATHSGTGWRITWRPGLEGAS
HCCCCCHHHCCCCEEEECCCHHHEECCCCCCCCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
ASSRKPKVADSSADLVNKVAQRVAACAAFVVSGEIETPTVAIALSGGRDSAALLHACAA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHH
WRDAGARVQLVALHIHHGLQADADVWECACQRMAQAAGVAFHARRVYVEIDAGQGVEEAA
HHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEEEEEECCCCHHHHH
REARYAALEALCAQTGASILLTAHHQDDQAETVLLQLMRGAGLDGLAAMPMARAGAVTLL
HHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHCCHHHCCHHHEE
RPWVDAPRSEIELYAHAHALCWVEDPSNTDARYARNALRPLLAGMADHFPAYREALSRSA
HHHHCCCHHHHHHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
AHLADAAALIDEIAQADLTRIASANGLDVAALIALSAPRQRAVLRAWLTKAGMRALSSRR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LEDLHTQLIDARDDGALRIELPTGQVRRYRGVAWIDTGANDRPGQAAVAIGAQLFEPAHP
HHHHHHHHHCCCCCCEEEEECCCCHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHCCCCC
AEQRVTVDAWGGALLFAPVASDGVATQTLQAPLVLSPRRGGERIALRPGGPSRALKQAYQ
HHHEEEEEECCCEEEEEECCCCCCCHHHHCCCEEECCCCCCCEEEECCCCHHHHHHHHHH
EAGIPAWERQRLPLLYAGEMLVFAAGLGMNQAATHSGTGWRITWRPGLEGAS
HCCCCCHHHCCCCEEEECCCHHHEECCCCCCCCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]