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The map label for this gene is trmJ [H]

Identifier: 187928095

GI number: 187928095

Start: 1060028

End: 1060876

Strand: Direct

Name: trmJ [H]

Synonym: Rpic_1002

Alternate gene names: 187928095

Gene position: 1060028-1060876 (Clockwise)

Preceding gene: 187928093

Following gene: 187928096

Centisome position: 26.89

GC content: 68.9

Gene sequence:

>849_bases
ATGAGCACACCGAACACCACACCCGCCGACCCGGAAATCCTGCGCCAGCAAGCCACGCGGTGCCGCTTCGTGCTGGTCGA
GACCAGTCACCCGGGCAATGTCGGCTCCACGGCACGTGCGCTCAAGACCATGGGGTTTGGCGAGCCCGGTAGCTTCGTGC
TCGTCCGCCCGCGCGAGGCCGACGCAAAGCGCCACGCTGATGCCGTTGCCATGGCAAGTGGCGCCGATGACGTGCTGGCC
GCCGCCCGCGTCGTCGACGACATTGGCGAGGCGTTGGCCGGTGCCTCGCTCACGATTGCGCTGACGGCCCGCCCGCGCGA
ATTCGGTCCGCGCCGCATCGGCCCGCGTGCTGCGGCAGAAGAAGTGGCCGCATTGCTGGCCGCGCCGGACGCCACCGTCG
CCTTCGTCTTCGGCAACGAGCGATTCGGCTTGCCCAACGAGGTGGTGGAGCGCTGCCACGTCGTCACGCACATTCCAGCC
AACCCGGCGTATGCCTCGCTGAACCTGTCGCAGGCCGTGCAGTTGATTGCGTACGAGGTGCGCATGGCGCTGCTGGCCGG
CACCGGCACGGGCGACACGCGCATGGAAGGCGTCGGCTTTGTCGGCGAGCCGGCCACGGCCGAGCAGATCGACGGGATGT
TCGAACATCTGGAGCAGGGCTTGGTCGAGATCGGCTTTCTCGACCCGGCCCAGCCGAAAAAGCTGATGCCGCGCCTGCGA
CGTCTGTTCGCCCGCACGCAGCTGGAGACCGAAGAGGTCAACATCCTGCGCGGCATCGCCAAGCGCATGCTCGGCCGCCG
TGCCGAAGCCACCACGCCGCCCACCGGCGCCGCCGACGACTGTCGCTGA

Upstream 100 bases:

>100_bases
AAGAACGAGCGGCGGGCCAATCTTGCGATCCTGCCCGCCGTCAAAAGAGTGGCCGTATTGTATATGAACCCCGCCCCCCG
GCGCCCCGCGAATTGCGCCA

Downstream 100 bases:

>100_bases
TCGCCCAGAAAGCCGCCTCCTGGCTGATCCAGCAGGGCGGCATTTCCTGCATGTCTGGTTAAAACCCTTGCCGCAGCGCG
GTAAGGCAATGCCTTACACT

Product: TrmH family RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MSTPNTTPADPEILRQQATRCRFVLVETSHPGNVGSTARALKTMGFGEPGSFVLVRPREADAKRHADAVAMASGADDVLA
AARVVDDIGEALAGASLTIALTARPREFGPRRIGPRAAAEEVAALLAAPDATVAFVFGNERFGLPNEVVERCHVVTHIPA
NPAYASLNLSQAVQLIAYEVRMALLAGTGTGDTRMEGVGFVGEPATAEQIDGMFEHLEQGLVEIGFLDPAQPKKLMPRLR
RLFARTQLETEEVNILRGIAKRMLGRRAEATTPPTGAADDCR

Sequences:

>Translated_282_residues
MSTPNTTPADPEILRQQATRCRFVLVETSHPGNVGSTARALKTMGFGEPGSFVLVRPREADAKRHADAVAMASGADDVLA
AARVVDDIGEALAGASLTIALTARPREFGPRRIGPRAAAEEVAALLAAPDATVAFVFGNERFGLPNEVVERCHVVTHIPA
NPAYASLNLSQAVQLIAYEVRMALLAGTGTGDTRMEGVGFVGEPATAEQIDGMFEHLEQGLVEIGFLDPAQPKKLMPRLR
RLFARTQLETEEVNILRGIAKRMLGRRAEATTPPTGAADDCR
>Mature_281_residues
STPNTTPADPEILRQQATRCRFVLVETSHPGNVGSTARALKTMGFGEPGSFVLVRPREADAKRHADAVAMASGADDVLAA
ARVVDDIGEALAGASLTIALTARPREFGPRRIGPRAAAEEVAALLAAPDATVAFVFGNERFGLPNEVVERCHVVTHIPAN
PAYASLNLSQAVQLIAYEVRMALLAGTGTGDTRMEGVGFVGEPATAEQIDGMFEHLEQGLVEIGFLDPAQPKKLMPRLRR
LFARTQLETEEVNILRGIAKRMLGRRAEATTPPTGAADDCR

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=244, Percent_Identity=45.4918032786885, Blast_Score=185, Evalue=3e-48,
Organism=Escherichia coli, GI1790865, Length=166, Percent_Identity=31.9277108433735, Blast_Score=71, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30073; Mature: 29942

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPNTTPADPEILRQQATRCRFVLVETSHPGNVGSTARALKTMGFGEPGSFVLVRPREA
CCCCCCCCCCHHHHHHHHHCEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEECCHH
DAKRHADAVAMASGADDVLAAARVVDDIGEALAGASLTIALTARPREFGPRRIGPRAAAE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCHHCCCCCCCCHHHHH
EVAALLAAPDATVAFVFGNERFGLPNEVVERCHVVTHIPANPAYASLNLSQAVQLIAYEV
HHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHEEECCCCCCEEECCHHHHHHHHHHHH
RMALLAGTGTGDTRMEGVGFVGEPATAEQIDGMFEHLEQGLVEIGFLDPAQPKKLMPRLR
HHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHH
RLFARTQLETEEVNILRGIAKRMLGRRAEATTPPTGAADDCR
HHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
STPNTTPADPEILRQQATRCRFVLVETSHPGNVGSTARALKTMGFGEPGSFVLVRPREA
CCCCCCCCCHHHHHHHHHCEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEECCHH
DAKRHADAVAMASGADDVLAAARVVDDIGEALAGASLTIALTARPREFGPRRIGPRAAAE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCHHCCCCCCCCHHHHH
EVAALLAAPDATVAFVFGNERFGLPNEVVERCHVVTHIPANPAYASLNLSQAVQLIAYEV
HHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHEEECCCCCCEEECCHHHHHHHHHHHH
RMALLAGTGTGDTRMEGVGFVGEPATAEQIDGMFEHLEQGLVEIGFLDPAQPKKLMPRLR
HHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHH
RLFARTQLETEEVNILRGIAKRMLGRRAEATTPPTGAADDCR
HHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]