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The map label for this gene is eno [H]

Identifier: 187928065

GI number: 187928065

Start: 1030165

End: 1031448

Strand: Direct

Name: eno [H]

Synonym: Rpic_0972

Alternate gene names: 187928065

Gene position: 1030165-1031448 (Clockwise)

Preceding gene: 187928064

Following gene: 187928066

Centisome position: 26.13

GC content: 61.29

Gene sequence:

>1284_bases
ATGAGTGCCATCGTAGATATCATCGGTCGCGAAGTGCTGGACTCGCGCGGCAACCCCACCGTCGAATGCGACGTGCTGTT
GGAATCGGGCGTGATGGGCCGTGCCGCGGTGCCGTCGGGCGCATCCACCGGCTCGCGCGAAGCCATCGAACTGCGTGACG
GCGACAAGTCGCGCTACCTGGGCAAGGGCGTGCTCAAGGCCGTCGAGCACATCAACACCGAAATCTCCGAGGCCATCATG
GGCCTGGACGCGTCGGAACAGGCTTTCCTGGACCGTACCCTGATCGATCTGGACGGTACCGAGAACAAGAGCCGCCTGGG
CGCGAACGCCATGCTGGCGGTGTCGATGGCGGTTGCCAAGGCTGCCGCTGAAGAAGCCGGCCTGCCGCTGTACCGCTACT
TCGGCGGTTCGGGCGCGATGCAGATGCCGGTGCCGATGATGAACATCGTCAACGGCGGTGCACACGCCAACAACAGCCTG
GACATCCAGGAATTCATGGTGATGCCGGTTGGCCAGTCGAGCTTCCGTGAAGCCCTGCGCTGCGGTGCAGAGATCTTTCA
CGCGCTGAAGAAGATCCTGGCTGACAAGGGTATGAGCACGGCCGTAGGCGATGAAGGTGGCTTCGCACCGAACTTCGCCA
GCAACGAAGAATGCCTGAACACCATCCTGTCGGCCATCGAGCAGGCTGGCTACAAGGCCGGCGAAGACGTGCTGCTGGCG
CTCGACTGCGCAGCTTCCGAGTTCTACAAGGACGGCAAGTACCACCTCGAAGGCGAAGGCCTCCAACTGTCGTCGGAAGA
CTTTGCCAACTACCTGGCGAACCTGGCTGACAAGTTCCCCATCGTGTCGATTGAAGACGGCATGCACGAGAGCGACTGGG
CGGGCTGGAAGGCACTGACCGATAAGCTCGGCAAGAAGGTGCAACTGGTGGGCGACGACTTGTTCGTCACCAACACGAAG
ATCCTGAAGGAAGGCATCGAGAAGGGCATCGCCAACTCGATCCTCATCAAGATCAATCAGATCGGTACGCTGACCGAGAC
GTTCGCGGCCATCGAGATGGCCAAGCGCGCTGGCTACACCGCCGTCATCTCGCACCGTTCGGGCGAAACCGAAGACAGCA
CGATCGCCGACATCGCCGTTGGCACGAACGCTGGCCAGATCAAGACCGGCTCGCTGTCGCGCTCGGACCGCATGGCCAAG
TACAACCAACTGCTGCGCATCGAAGAAGACCTCGGTGATATCGCCAGCTACCCGGGCAAGTCGGCGTTCTATAACCTGCG
ATAA

Upstream 100 bases:

>100_bases
CGCCAACGCTGCCAACATGAACATGATCGTGGTCGAAGGCATTTAACCTGCCTGTCGATCTCGTAGCGTTACCTGCTTTA
AGACACCAAGAGGGATTTTC

Downstream 100 bases:

>100_bases
TTCCTCGCAGTTTTGCCCTGCATGACTGTGCGACCGCCGCTCTGGCGGTCGCATGGCATTTGGGGCCCGAGTCCTGCCAA
CGCTTGTCTCTATGCGCCTG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase 1; 2-phosphoglycerate dehydratase 1 [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSAIVDIIGREVLDSRGNPTVECDVLLESGVMGRAAVPSGASTGSREAIELRDGDKSRYLGKGVLKAVEHINTEISEAIM
GLDASEQAFLDRTLIDLDGTENKSRLGANAMLAVSMAVAKAAAEEAGLPLYRYFGGSGAMQMPVPMMNIVNGGAHANNSL
DIQEFMVMPVGQSSFREALRCGAEIFHALKKILADKGMSTAVGDEGGFAPNFASNEECLNTILSAIEQAGYKAGEDVLLA
LDCAASEFYKDGKYHLEGEGLQLSSEDFANYLANLADKFPIVSIEDGMHESDWAGWKALTDKLGKKVQLVGDDLFVTNTK
ILKEGIEKGIANSILIKINQIGTLTETFAAIEMAKRAGYTAVISHRSGETEDSTIADIAVGTNAGQIKTGSLSRSDRMAK
YNQLLRIEEDLGDIASYPGKSAFYNLR

Sequences:

>Translated_427_residues
MSAIVDIIGREVLDSRGNPTVECDVLLESGVMGRAAVPSGASTGSREAIELRDGDKSRYLGKGVLKAVEHINTEISEAIM
GLDASEQAFLDRTLIDLDGTENKSRLGANAMLAVSMAVAKAAAEEAGLPLYRYFGGSGAMQMPVPMMNIVNGGAHANNSL
DIQEFMVMPVGQSSFREALRCGAEIFHALKKILADKGMSTAVGDEGGFAPNFASNEECLNTILSAIEQAGYKAGEDVLLA
LDCAASEFYKDGKYHLEGEGLQLSSEDFANYLANLADKFPIVSIEDGMHESDWAGWKALTDKLGKKVQLVGDDLFVTNTK
ILKEGIEKGIANSILIKINQIGTLTETFAAIEMAKRAGYTAVISHRSGETEDSTIADIAVGTNAGQIKTGSLSRSDRMAK
YNQLLRIEEDLGDIASYPGKSAFYNLR
>Mature_426_residues
SAIVDIIGREVLDSRGNPTVECDVLLESGVMGRAAVPSGASTGSREAIELRDGDKSRYLGKGVLKAVEHINTEISEAIMG
LDASEQAFLDRTLIDLDGTENKSRLGANAMLAVSMAVAKAAAEEAGLPLYRYFGGSGAMQMPVPMMNIVNGGAHANNSLD
IQEFMVMPVGQSSFREALRCGAEIFHALKKILADKGMSTAVGDEGGFAPNFASNEECLNTILSAIEQAGYKAGEDVLLAL
DCAASEFYKDGKYHLEGEGLQLSSEDFANYLANLADKFPIVSIEDGMHESDWAGWKALTDKLGKKVQLVGDDLFVTNTKI
LKEGIEKGIANSILIKINQIGTLTETFAAIEMAKRAGYTAVISHRSGETEDSTIADIAVGTNAGQIKTGSLSRSDRMAKY
NQLLRIEEDLGDIASYPGKSAFYNLR

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=441, Percent_Identity=51.2471655328798, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI4503571, Length=431, Percent_Identity=49.4199535962877, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI301897477, Length=437, Percent_Identity=50.3432494279176, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI301897469, Length=437, Percent_Identity=50.3432494279176, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI301897479, Length=428, Percent_Identity=45.7943925233645, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=25.5952380952381, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=25.5952380952381, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=25.5952380952381, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1789141, Length=425, Percent_Identity=67.5294117647059, Blast_Score=573, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI17536383, Length=437, Percent_Identity=50.5720823798627, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI71995829, Length=437, Percent_Identity=50.5720823798627, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32563855, Length=197, Percent_Identity=45.1776649746193, Blast_Score=171, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6321693, Length=434, Percent_Identity=47.4654377880184, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6323985, Length=432, Percent_Identity=48.8425925925926, Blast_Score=385, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6324974, Length=432, Percent_Identity=48.8425925925926, Blast_Score=384, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6324969, Length=432, Percent_Identity=48.8425925925926, Blast_Score=384, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6321968, Length=434, Percent_Identity=47.2350230414747, Blast_Score=363, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580918, Length=428, Percent_Identity=50.7009345794392, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580916, Length=428, Percent_Identity=50.7009345794392, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580920, Length=428, Percent_Identity=50.7009345794392, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580914, Length=428, Percent_Identity=50.7009345794392, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI281360527, Length=428, Percent_Identity=50.7009345794392, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI17137654, Length=428, Percent_Identity=50.7009345794392, Blast_Score=399, Evalue=1e-111,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45641; Mature: 45510

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIVDIIGREVLDSRGNPTVECDVLLESGVMGRAAVPSGASTGSREAIELRDGDKSRYL
CCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHH
GKGVLKAVEHINTEISEAIMGLDASEQAFLDRTLIDLDGTENKSRLGANAMLAVSMAVAK
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEECCCCCCHHHHCCHHHHHHHHHHHH
AAAEEAGLPLYRYFGGSGAMQMPVPMMNIVNGGAHANNSLDIQEFMVMPVGQSSFREALR
HHHHHCCCCEEEEECCCCCEECCCHHHHHHCCCCCCCCCCCHHHHHCCCCCHHHHHHHHH
CGAEIFHALKKILADKGMSTAVGDEGGFAPNFASNEECLNTILSAIEQAGYKAGEDVLLA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDCAASEFYKDGKYHLEGEGLQLSSEDFANYLANLADKFPIVSIEDGMHESDWAGWKALT
EEHHHHHHHCCCCEEECCCCCEECHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHH
DKLGKKVQLVGDDLFVTNTKILKEGIEKGIANSILIKINQIGTLTETFAAIEMAKRAGYT
HHHCCEEEEECCCEEECHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCE
AVISHRSGETEDSTIADIAVGTNAGQIKTGSLSRSDRMAKYNQLLRIEEDLGDIASYPGK
EEEECCCCCCCCCEEEEEEECCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
SAFYNLR
CCCCCCC
>Mature Secondary Structure 
SAIVDIIGREVLDSRGNPTVECDVLLESGVMGRAAVPSGASTGSREAIELRDGDKSRYL
CHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHH
GKGVLKAVEHINTEISEAIMGLDASEQAFLDRTLIDLDGTENKSRLGANAMLAVSMAVAK
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEECCCCCCHHHHCCHHHHHHHHHHHH
AAAEEAGLPLYRYFGGSGAMQMPVPMMNIVNGGAHANNSLDIQEFMVMPVGQSSFREALR
HHHHHCCCCEEEEECCCCCEECCCHHHHHHCCCCCCCCCCCHHHHHCCCCCHHHHHHHHH
CGAEIFHALKKILADKGMSTAVGDEGGFAPNFASNEECLNTILSAIEQAGYKAGEDVLLA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDCAASEFYKDGKYHLEGEGLQLSSEDFANYLANLADKFPIVSIEDGMHESDWAGWKALT
EEHHHHHHHCCCCEEECCCCCEECHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHH
DKLGKKVQLVGDDLFVTNTKILKEGIEKGIANSILIKINQIGTLTETFAAIEMAKRAGYT
HHHCCEEEEECCCEEECHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCE
AVISHRSGETEDSTIADIAVGTNAGQIKTGSLSRSDRMAKYNQLLRIEEDLGDIASYPGK
EEEECCCCCCCCCEEEEEEECCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
SAFYNLR
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA