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The map label for this gene is mexA [H]

Identifier: 187927936

GI number: 187927936

Start: 891225

End: 892406

Strand: Direct

Name: mexA [H]

Synonym: Rpic_0841

Alternate gene names: 187927936

Gene position: 891225-892406 (Clockwise)

Preceding gene: 187927935

Following gene: 187927937

Centisome position: 22.61

GC content: 67.94

Gene sequence:

>1182_bases
ATGATGTTCTCCGTGTTGTTCCGTCGTTACCGTTCGTTCCGGTGGTTGCTGTGCGCGTGCGCCTTGGCTGTGTTGCAGGC
GTGCGGCAAGCATGCTGATGAGGCTGCCAAGCCAGCCGGCCCGCCGGAAGTCGAGTTTGCCGTTGCGCAGCGCTCCGATG
CGCCGCTGGTGACCGAACTGCCTGGCCGCCTGGAGCCGTATCGCACGGCGCAAGTGCGCGCGCGCGTCGACGGCATCGTC
CTCAAGCGCGCCTATGAAGAAGGCCAGGTGGTCAAGGCCGGCCAGGTGTTGTTCCGCATCGATCCGGCGCCGCTCAAGGC
ACAGGTGGACGCCGCGCAAGGCGCGCTTGCCCGTGCGCAGGCACAACTGGCCATCGCGCGCGACAAGGCTGCCCGCTACA
AGGGGCTGGCCGCCACGCGTGCCGTGAGCGAGTTGGAATACGCTGAAGCGCAATCCGCCGAGCGTCAGGCCGCCGCAGAC
GTGGTGTCCAGCCGTGCCGCGCTGGAAACCGCGCAACTGCGCTTGGGCTATGCCACCGTCACGGCCCCGATTGCCGGCCG
CTCGCGCCGCGCGCAGGTCACCGAAGGTGCGCTGGTCAAGGAAGACGCTGCGACGACGCTCACCACGGTCGAACAGATCG
ACCCGATCTACGTCGACTTCGCGCAGCCCGCTGCCGAAGTGCTCGCCTTGCAGCGCGCGCTCAAGAAGGGCCAGGCCGCT
GCATTGAGCGGCCAGGACATCGGCGTGCAGGTCATCCTGAGCGATGGCGTGGCGTACGAGCGCCCGGGCAGGCTGCTGTT
CTCCGATCTGACGGTGGACCGTGCGACGGATAACGTCTCGATGCGCGCGGTGCTGCCCAATCCCGATGGCCTGCTGCTGC
CCGGCATGTACGTGCGGGTGAAGCTTTCGCACGCCGTGCAGCAGGGCGCGGTGACGGTTCCGAAGGGCGCGTTGCAGCGC
GACCGCCAGGGGGCATCGGTGTTTGTGGTGCAGGCCGACAATACGCTGGCGGCCCAGCCCGTGGCTGCGCAAACGTTGGC
CGGTGACCAATGGCTGGTGACGGGCGGGCTATCCGGTGGGGAGCGGGTTGCCATCCCAGGCATGCAGACGCTCGAGACCG
GCATGGCAGTCAAGCCGATTCCTGCCGCACCGCGCGCTGATACGAAGACGAAGAGGGGCTGA

Upstream 100 bases:

>100_bases
TGGGGAAATCAATCGGTCAGCTAGCGGCGGACGTGCGTGCTAGCCTTCCGCTTCGCTTCGTTCAAGACCTCGGGCGCGGT
CGCGCCCAGCCCTCTTTCCC

Downstream 100 bases:

>100_bases
CCATGGCGCGCTTCTTTATCGACCGCCCGGTCTTTGCCTGGGTGATTGCGCTGGCCATCATCCTGGCCGGCGTGCTCGCG
CTGCCCAAGCTGCCGGTGGC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MMFSVLFRRYRSFRWLLCACALAVLQACGKHADEAAKPAGPPEVEFAVAQRSDAPLVTELPGRLEPYRTAQVRARVDGIV
LKRAYEEGQVVKAGQVLFRIDPAPLKAQVDAAQGALARAQAQLAIARDKAARYKGLAATRAVSELEYAEAQSAERQAAAD
VVSSRAALETAQLRLGYATVTAPIAGRSRRAQVTEGALVKEDAATTLTTVEQIDPIYVDFAQPAAEVLALQRALKKGQAA
ALSGQDIGVQVILSDGVAYERPGRLLFSDLTVDRATDNVSMRAVLPNPDGLLLPGMYVRVKLSHAVQQGAVTVPKGALQR
DRQGASVFVVQADNTLAAQPVAAQTLAGDQWLVTGGLSGGERVAIPGMQTLETGMAVKPIPAAPRADTKTKRG

Sequences:

>Translated_393_residues
MMFSVLFRRYRSFRWLLCACALAVLQACGKHADEAAKPAGPPEVEFAVAQRSDAPLVTELPGRLEPYRTAQVRARVDGIV
LKRAYEEGQVVKAGQVLFRIDPAPLKAQVDAAQGALARAQAQLAIARDKAARYKGLAATRAVSELEYAEAQSAERQAAAD
VVSSRAALETAQLRLGYATVTAPIAGRSRRAQVTEGALVKEDAATTLTTVEQIDPIYVDFAQPAAEVLALQRALKKGQAA
ALSGQDIGVQVILSDGVAYERPGRLLFSDLTVDRATDNVSMRAVLPNPDGLLLPGMYVRVKLSHAVQQGAVTVPKGALQR
DRQGASVFVVQADNTLAAQPVAAQTLAGDQWLVTGGLSGGERVAIPGMQTLETGMAVKPIPAAPRADTKTKRG
>Mature_393_residues
MMFSVLFRRYRSFRWLLCACALAVLQACGKHADEAAKPAGPPEVEFAVAQRSDAPLVTELPGRLEPYRTAQVRARVDGIV
LKRAYEEGQVVKAGQVLFRIDPAPLKAQVDAAQGALARAQAQLAIARDKAARYKGLAATRAVSELEYAEAQSAERQAAAD
VVSSRAALETAQLRLGYATVTAPIAGRSRRAQVTEGALVKEDAATTLTTVEQIDPIYVDFAQPAAEVLALQRALKKGQAA
ALSGQDIGVQVILSDGVAYERPGRLLFSDLTVDRATDNVSMRAVLPNPDGLLLPGMYVRVKLSHAVQQGAVTVPKGALQR
DRQGASVFVVQADNTLAAQPVAAQTLAGDQWLVTGGLSGGERVAIPGMQTLETGMAVKPIPAAPRADTKTKRG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=378, Percent_Identity=39.4179894179894, Blast_Score=258, Evalue=6e-70,
Organism=Escherichia coli, GI1786668, Length=375, Percent_Identity=41.3333333333333, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1789929, Length=389, Percent_Identity=37.7892030848329, Blast_Score=251, Evalue=4e-68,
Organism=Escherichia coli, GI87082036, Length=321, Percent_Identity=30.8411214953271, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI87081796, Length=275, Percent_Identity=27.6363636363636, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41665; Mature: 41665

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMFSVLFRRYRSFRWLLCACALAVLQACGKHADEAAKPAGPPEVEFAVAQRSDAPLVTEL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCEEEEEECCCCCCCCCCC
PGRLEPYRTAQVRARVDGIVLKRAYEEGQVVKAGQVLFRIDPAPLKAQVDAAQGALARAQ
CCCCCCCHHHHHHHHHHHHHEEHHHCCCCEEEECEEEEEECCCCCEEECHHHHHHHHHHH
AQLAIARDKAARYKGLAATRAVSELEYAEAQSAERQAAADVVSSRAALETAQLRLGYATV
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECEEEE
TAPIAGRSRRAQVTEGALVKEDAATTLTTVEQIDPIYVDFAQPAAEVLALQRALKKGQAA
EECCCCCCCCCCCCCCCEEECCCHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHCCCEE
ALSGQDIGVQVILSDGVAYERPGRLLFSDLTVDRATDNVSMRAVLPNPDGLLLPGMYVRV
EECCCCCCEEEEEECCCCCCCCCCEEEEECEEECCCCCEEEEEECCCCCCEECCCEEEEE
KLSHAVQQGAVTVPKGALQRDRQGASVFVVQADNTLAAQPVAAQTLAGDQWLVTGGLSGG
EEHHHHHCCCEECCCHHHHHCCCCCEEEEEECCCCCCCCCCHHHHCCCCCEEEEECCCCC
ERVAIPGMQTLETGMAVKPIPAAPRADTKTKRG
CEEECCCHHHHHCCCEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMFSVLFRRYRSFRWLLCACALAVLQACGKHADEAAKPAGPPEVEFAVAQRSDAPLVTEL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCEEEEEECCCCCCCCCCC
PGRLEPYRTAQVRARVDGIVLKRAYEEGQVVKAGQVLFRIDPAPLKAQVDAAQGALARAQ
CCCCCCCHHHHHHHHHHHHHEEHHHCCCCEEEECEEEEEECCCCCEEECHHHHHHHHHHH
AQLAIARDKAARYKGLAATRAVSELEYAEAQSAERQAAADVVSSRAALETAQLRLGYATV
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECEEEE
TAPIAGRSRRAQVTEGALVKEDAATTLTTVEQIDPIYVDFAQPAAEVLALQRALKKGQAA
EECCCCCCCCCCCCCCCEEECCCHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHCCCEE
ALSGQDIGVQVILSDGVAYERPGRLLFSDLTVDRATDNVSMRAVLPNPDGLLLPGMYVRV
EECCCCCCEEEEEECCCCCCCCCCEEEEECEEECCCCCEEEEEECCCCCCEECCCEEEEE
KLSHAVQQGAVTVPKGALQRDRQGASVFVVQADNTLAAQPVAAQTLAGDQWLVTGGLSGG
EEHHHHHCCCEECCCHHHHHCCCCCEEEEEECCCCCCCCCCHHHHCCCCCEEEEECCCCC
ERVAIPGMQTLETGMAVKPIPAAPRADTKTKRG
CEEECCCHHHHHCCCEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]