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The map label for this gene is glcA [H]

Identifier: 187927932

GI number: 187927932

Start: 885894

End: 887570

Strand: Reverse

Name: glcA [H]

Synonym: Rpic_0837

Alternate gene names: 187927932

Gene position: 887570-885894 (Counterclockwise)

Preceding gene: 187927938

Following gene: 187927931

Centisome position: 22.51

GC content: 64.76

Gene sequence:

>1677_bases
ATGTGGAATCAAGTCTATGACCCGCTCGGCAGTGCCGTGTGGTCAACCCTAGCGGCCGGGATACCGGTGGCGGTACTGCT
GTGCTCGCTGGCGTTCTTCCATATGCAGGCGCATCTGGCAGCCGGCCTGGCGCTGATTGTCGGGGTGGGGATCGCCTCGT
TTGTGTTTGGCATGCCGACCGCAATGGCTGGCAAGGCTGCGGGCCTCGGCATCGTCTCGGGCCTGTTCCCAATTGGCTGG
ATTGTTCTCAACATCATCTTCCTGCACCGGCTCACCACCTTGAACGGGTCGTTCAAGGTCTTGCAGAGCTCGATTTCCGG
CATTACCGAAGACCGCCGCCTGCAGCTGCTGCTGGTAGCGTTCAGCTTTGGCGCGTTCTTTGAAGGCGCCGCGGGCTTCG
GCACGCCGGTGGCCGTCACGGGCGCCATCCTGATCGGCCTGGGCTTCTCGCCACTGGCCGCGTCGGGCCTGGCCCTGATC
GCCAACACCGCACCGGTGGCCTACGGCGCGCTGGGTGCCCCGCTCATCGGGCTGGCTTCGGTCACGGGGCTGGACCTGCT
GCAGCTCTCTGCCATGGTTGGCCGCCAGTTGCCGTTCTTCTCGGTGCTGGTGCCGTTCTGGCTGATCTGGGCGTTCGCGG
GCTTCCGCGGCATGCTGCAGATCTGGCCAGCAATCCTGGTGGCGGGCGTGTCGTTTGCGATTCCGCAGTTCCTGGTGTCG
AACTTCCACGGCCCGTGGCTGGTGGACGTGATTGCGGCGCTGGTGTCGATGGGCACGCTCACGCTGTTCCTGAAGGTGTG
GAAGCCCAAGACGATCTGGACCTCGACCGCCCTGCGCAACCGCGAAGACAACTCCCGCGTCGACCCCGAAGCTGCTGCCG
AAGCCCGCGAAACCACCACGGCCACCAACGGCAAGATCACGCGCGTGCAGGCATGGTTGCCGTGGGTCATCCTGACCGTG
TTCGTGTTCATCTGGGGCGTGCCGCAGTTCAAGGCGTTTGCCGACGGCCTCTGGGCATTCAAGTTCGCCATCCCGGGCCT
CGACAAGATGGTGCTCAAGGGCCCGCCGGTGGTGCCCAAGGTGATTGCCGAAGGCGCAGTGTTCAACTTCAACGTCCTGT
CGATGGCGGGTACGGGCATCCTGGTATCGGCCATCGTCGGCGGCCTGCTGATGGGCTACTCGCTGCCGCGCATGCTCAAG
GAGTACTGGAACACCATCAAGCTCACGCGCTATTCGCTGCTGACGATCTGCGCGATGTTCGGCATCGGCTACCTGACGCG
CTACTCCGGTCTGGACGCGACGCTGGGCCTGGCCTTCGCCCACACGGGCGTGCTGTACCCGCTGTTCGGCACGATGCTGG
GCTGGCTGGGCGTGGCGCTCACCGGCTCCGATACGGCATCGAACGTGCTGTTCGGCGGCCTGCAGAAGACCACGGCCGAA
CAGCTTGGCCTGTCGCCGATCCTCATGTCGGCGGCCAACAGTTCGGGCGGCGTGATGGGCAAGATGATCGACGCGCAGTC
CATCGTCGTGGCTTCCACCGCCACCAAGTGGTACGGCCACGAGGGCGACATCCTGCGCTACGTGTTCTTCCACTCGCTGG
CGCTGGCCTTCCTGGTGGGGCTGATGATCACCCTGCAGGCTTATGTGGAGCCGTTCTCGCGGATGGTGGTGCACTGA

Upstream 100 bases:

>100_bases
CGCTTGTTCCTACAATCCGTGGCTGCCCCTTAAAAAAGGGGCGACAGAAGACGTTCGGTATCGAGAAGATACTGAAACGC
CCAAACAGGAGGAGATCAAG

Downstream 100 bases:

>100_bases
GCCCCGCTCCCGACGGAACCCGAAAGCCGCTCAATCGAGCGGCTTTTTTCATGGCGCGATGCGTCTCGCGAACCGCCTGG
CGCCTCCCACGGATCGCAGC

Product: L-lactate transport

Products: lactate [Cytoplasm]; Proton [Cytoplasm]; glycolate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 558; Mature: 558

Protein sequence:

>558_residues
MWNQVYDPLGSAVWSTLAAGIPVAVLLCSLAFFHMQAHLAAGLALIVGVGIASFVFGMPTAMAGKAAGLGIVSGLFPIGW
IVLNIIFLHRLTTLNGSFKVLQSSISGITEDRRLQLLLVAFSFGAFFEGAAGFGTPVAVTGAILIGLGFSPLAASGLALI
ANTAPVAYGALGAPLIGLASVTGLDLLQLSAMVGRQLPFFSVLVPFWLIWAFAGFRGMLQIWPAILVAGVSFAIPQFLVS
NFHGPWLVDVIAALVSMGTLTLFLKVWKPKTIWTSTALRNREDNSRVDPEAAAEARETTTATNGKITRVQAWLPWVILTV
FVFIWGVPQFKAFADGLWAFKFAIPGLDKMVLKGPPVVPKVIAEGAVFNFNVLSMAGTGILVSAIVGGLLMGYSLPRMLK
EYWNTIKLTRYSLLTICAMFGIGYLTRYSGLDATLGLAFAHTGVLYPLFGTMLGWLGVALTGSDTASNVLFGGLQKTTAE
QLGLSPILMSAANSSGGVMGKMIDAQSIVVASTATKWYGHEGDILRYVFFHSLALAFLVGLMITLQAYVEPFSRMVVH

Sequences:

>Translated_558_residues
MWNQVYDPLGSAVWSTLAAGIPVAVLLCSLAFFHMQAHLAAGLALIVGVGIASFVFGMPTAMAGKAAGLGIVSGLFPIGW
IVLNIIFLHRLTTLNGSFKVLQSSISGITEDRRLQLLLVAFSFGAFFEGAAGFGTPVAVTGAILIGLGFSPLAASGLALI
ANTAPVAYGALGAPLIGLASVTGLDLLQLSAMVGRQLPFFSVLVPFWLIWAFAGFRGMLQIWPAILVAGVSFAIPQFLVS
NFHGPWLVDVIAALVSMGTLTLFLKVWKPKTIWTSTALRNREDNSRVDPEAAAEARETTTATNGKITRVQAWLPWVILTV
FVFIWGVPQFKAFADGLWAFKFAIPGLDKMVLKGPPVVPKVIAEGAVFNFNVLSMAGTGILVSAIVGGLLMGYSLPRMLK
EYWNTIKLTRYSLLTICAMFGIGYLTRYSGLDATLGLAFAHTGVLYPLFGTMLGWLGVALTGSDTASNVLFGGLQKTTAE
QLGLSPILMSAANSSGGVMGKMIDAQSIVVASTATKWYGHEGDILRYVFFHSLALAFLVGLMITLQAYVEPFSRMVVH
>Mature_558_residues
MWNQVYDPLGSAVWSTLAAGIPVAVLLCSLAFFHMQAHLAAGLALIVGVGIASFVFGMPTAMAGKAAGLGIVSGLFPIGW
IVLNIIFLHRLTTLNGSFKVLQSSISGITEDRRLQLLLVAFSFGAFFEGAAGFGTPVAVTGAILIGLGFSPLAASGLALI
ANTAPVAYGALGAPLIGLASVTGLDLLQLSAMVGRQLPFFSVLVPFWLIWAFAGFRGMLQIWPAILVAGVSFAIPQFLVS
NFHGPWLVDVIAALVSMGTLTLFLKVWKPKTIWTSTALRNREDNSRVDPEAAAEARETTTATNGKITRVQAWLPWVILTV
FVFIWGVPQFKAFADGLWAFKFAIPGLDKMVLKGPPVVPKVIAEGAVFNFNVLSMAGTGILVSAIVGGLLMGYSLPRMLK
EYWNTIKLTRYSLLTICAMFGIGYLTRYSGLDATLGLAFAHTGVLYPLFGTMLGWLGVALTGSDTASNVLFGGLQKTTAE
QLGLSPILMSAANSSGGVMGKMIDAQSIVVASTATKWYGHEGDILRYVFFHSLALAFLVGLMITLQAYVEPFSRMVVH

Specific function: Transports glycolate across the membrane. Can also transport L-lactate and D-lactate. Seems to be driven by a proton motive force [H]

COG id: COG1620

COG function: function code C; L-lactate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lactate permease family [H]

Homologues:

Organism=Escherichia coli, GI1789347, Length=562, Percent_Identity=46.797153024911, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI1790031, Length=554, Percent_Identity=43.5018050541516, Blast_Score=412, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003804 [H]

Pfam domain/function: PF02652 Lactate_perm [H]

EC number: NA

Molecular weight: Translated: 59353; Mature: 59353

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactate [Periplasm]; Proton [Periplasm]; glycolate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactate [Periplasm] = Proton [Cytoplasm] + lactate [Cytoplasm] Proton [Periplasm] + glycolate [Periplasm] = Proton [Cytoplasm] + glycolate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 11283302; 11785976 [H]