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The map label for this gene is trmD [H]

Identifier: 187927901

GI number: 187927901

Start: 851448

End: 852236

Strand: Direct

Name: trmD [H]

Synonym: Rpic_0806

Alternate gene names: 187927901

Gene position: 851448-852236 (Clockwise)

Preceding gene: 187927900

Following gene: 187927902

Centisome position: 21.6

GC content: 64.13

Gene sequence:

>789_bases
ATGCAGTTCGACGTCGTCTCGCTGTTTCCGGACATGTTCCGGGCGCTGACGGACTGGGGTATCACCAGCCGCGCAGCCAA
ACAGCAGGTGTACACGTTGCGCACCTGGAACCCGCGCGATTTCACGACGGACAACTACCGCACCGTGGACGACCGCCCGT
ACGGCGGTGGCCCGGGCATGGTGATGTTGGCGAAGCCGCTAGAGGCCGCGCTGGATGCGATTCAAGCGGCGCAAGCGCCG
GCGGCTTCGCATGTGGTGTTGCTGTCGCCGCAAGGCAAGCCGCTCACGCACAAGCGGGTGATGGAACTGGCGCAGTTGCC
GGCGCTCACGTTGCTGTGCGGACGCTACGAAGCGATTGATCAACGTCTGGTCGACCGCCGCGTGGATGAGGAAATCAGCC
TTGGCGATTTCGTCCTCTCCGGCGGTGAGATTGCAGCAATGGCGATCATCGACGCCGTGGTGCGGCAGTTGCCCGGCGTG
CTGGGCGATGCACAGTCGGCGGTGCAAGACAGTTTCGTCAACGGCTTGCTCGACTGCCCGCACTACACGCGGCCGGAAGA
GTACGAAAGTGTGCGTGTGCCCGATGTGCTGCTGGGTGGCCACCATGCCGAGATCGAGAAATGGCGCCGCCAGCAGGCGC
TGGTGAACACGATGCGCAAGCGGCCCGATCTGATCGAGGCGGCGCGCAAGCAGGGTTTGTTGTCCCGCAGCGATGAGGTC
TTCCTCGCTGCCGCGGCGATAACGGCAAAGGGGTCAGAGCCTTCCATACCGGAAGGCTCGGCCAAGTGA

Upstream 100 bases:

>100_bases
CGTTTGTCGAGGTTTACGTGAAGTCGGTGGATGTCGCAGGCAAGCGCATCGTCGTCGACTGGGGTCTGGATTACTGAACA
TGCGGCCGGAGAATTCGGCG

Downstream 100 bases:

>100_bases
AGTTCCCATCCTCTACCGGGGCCACGGTCAACCGACTGGGCAAAACGCCGGCATGATGGTCAAGGAGAAAAAGATGAATC
TCATCGAGCAAATCGAGCAG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MQFDVVSLFPDMFRALTDWGITSRAAKQQVYTLRTWNPRDFTTDNYRTVDDRPYGGGPGMVMLAKPLEAALDAIQAAQAP
AASHVVLLSPQGKPLTHKRVMELAQLPALTLLCGRYEAIDQRLVDRRVDEEISLGDFVLSGGEIAAMAIIDAVVRQLPGV
LGDAQSAVQDSFVNGLLDCPHYTRPEEYESVRVPDVLLGGHHAEIEKWRRQQALVNTMRKRPDLIEAARKQGLLSRSDEV
FLAAAAITAKGSEPSIPEGSAK

Sequences:

>Translated_262_residues
MQFDVVSLFPDMFRALTDWGITSRAAKQQVYTLRTWNPRDFTTDNYRTVDDRPYGGGPGMVMLAKPLEAALDAIQAAQAP
AASHVVLLSPQGKPLTHKRVMELAQLPALTLLCGRYEAIDQRLVDRRVDEEISLGDFVLSGGEIAAMAIIDAVVRQLPGV
LGDAQSAVQDSFVNGLLDCPHYTRPEEYESVRVPDVLLGGHHAEIEKWRRQQALVNTMRKRPDLIEAARKQGLLSRSDEV
FLAAAAITAKGSEPSIPEGSAK
>Mature_262_residues
MQFDVVSLFPDMFRALTDWGITSRAAKQQVYTLRTWNPRDFTTDNYRTVDDRPYGGGPGMVMLAKPLEAALDAIQAAQAP
AASHVVLLSPQGKPLTHKRVMELAQLPALTLLCGRYEAIDQRLVDRRVDEEISLGDFVLSGGEIAAMAIIDAVVRQLPGV
LGDAQSAVQDSFVNGLLDCPHYTRPEEYESVRVPDVLLGGHHAEIEKWRRQQALVNTMRKRPDLIEAARKQGLLSRSDEV
FLAAAAITAKGSEPSIPEGSAK

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=226, Percent_Identity=55.3097345132743, Blast_Score=259, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 28759; Mature: 28759

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFDVVSLFPDMFRALTDWGITSRAAKQQVYTLRTWNPRDFTTDNYRTVDDRPYGGGPGM
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHEEEEECCCCCCCCCCCCEECCCCCCCCCCCE
VMLAKPLEAALDAIQAAQAPAASHVVLLSPQGKPLTHKRVMELAQLPALTLLCGRYEAID
EEEHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QRLVDRRVDEEISLGDFVLSGGEIAAMAIIDAVVRQLPGVLGDAQSAVQDSFVNGLLDCP
HHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCHHCHHHHHHHHHHHHHHHCCC
HYTRPEEYESVRVPDVLLGGHHAEIEKWRRQQALVNTMRKRPDLIEAARKQGLLSRSDEV
CCCCCCCCCCCCCCHHEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCE
FLAAAAITAKGSEPSIPEGSAK
EEEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MQFDVVSLFPDMFRALTDWGITSRAAKQQVYTLRTWNPRDFTTDNYRTVDDRPYGGGPGM
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHEEEEECCCCCCCCCCCCEECCCCCCCCCCCE
VMLAKPLEAALDAIQAAQAPAASHVVLLSPQGKPLTHKRVMELAQLPALTLLCGRYEAID
EEEHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QRLVDRRVDEEISLGDFVLSGGEIAAMAIIDAVVRQLPGVLGDAQSAVQDSFVNGLLDCP
HHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCHHCHHHHHHHHHHHHHHHCCC
HYTRPEEYESVRVPDVLLGGHHAEIEKWRRQQALVNTMRKRPDLIEAARKQGLLSRSDEV
CCCCCCCCCCCCCCHHEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCE
FLAAAAITAKGSEPSIPEGSAK
EEEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]