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The map label for this gene is pilJ [H]

Identifier: 187927711

GI number: 187927711

Start: 649798

End: 652035

Strand: Direct

Name: pilJ [H]

Synonym: Rpic_0615

Alternate gene names: 187927711

Gene position: 649798-652035 (Clockwise)

Preceding gene: 187927710

Following gene: 187927712

Centisome position: 16.48

GC content: 63.94

Gene sequence:

>2238_bases
ATGGGGTTCAAGTGGTTCAACGCAGGTCGCCGTGCCGGTGAGGCTGGGGCGCCCGAGGTTGCAGCGGAGGGCGCGTCGGC
GGCTTCCGTGCAGTTGGCGCAGTTGGACAATGCGTCCCATACGGATGACGATAATCCCGCTGGCACGCCCACGCAGCTCG
GCCGCTCGCCCATGGACGCCGTGGTCGGCTGGATGGAGCGCGTGCCGTTTGCCGCGCAGCAGCGTGTCTTCACGATCGGC
CTGGTGGTCGGTCTGCTGGCCCTGCTGATCTCGGTGTACCTGGATAACCGCCAGGCCAACAACGGTTCGGTGCAGATTGA
AATTGCCGGTGACACGCTGATGCACTCGCAGCGTCTGGCCAAGGCGGTGCCGGTGGCGCTGCTGGGTAACACCAACGCAT
TCGCCCAGCTCAAGGAGAGCCGCGCGCGTCTGCAGGAAAACCTCGATGCGTTGAAGAATGGCAACTCGGAGCGCGGTGTG
CGCGCCTCGGGTGGCGAGGCCGAAGCACTGCTCGACAAGTCGATCGAAGAGTGGAAGCGCAGCGACAAGAGCGCCCAGGC
CATCCTGGCGCAGGAAAAGACGCTGGTGGCGGTGGGTAAGACGCTGAACGTGTTCAACGCGTCGAACCCCGAACTGCTGG
AAGAAGCCGAGCAGATCTCGTCGATGAAGCTGCAGACCAACGCGCCGGCCCGTGAAGTGGCCGCCTCGTCGCAGCTGGTG
ATGTTGACGCAGCGTCTGGGTAAGAACATGAACGAGTTCCTGGCCGGCGAAGGCGTGAACCCGGAAACGGCGTTCCTGCT
CGGTAAGGACACCAACACCTTCCGCGAAACGCTGAACGGCCTGCTCAACGGCTCGGAAGCGCTGCGCCTGTCGGCTGCCA
CCGATGCCGAGACGAAGGGCTACCTGCAAGAGCTGTCGACCCGCTTCGACAACGTGCAGAAGACCACGCAGATCATTCTG
GAAAACCTGCCGGGCCTGATCGCCGCCAAGCGCGCCCAGCAGCAGATCTTCAACGACAACGAAAAGCTGCGTGCCGACCT
GTCTGACCTGCAGTCTGCCTATGCCGGCAGCGTGCGCAGCCGCCCCATGACGCTCGGTGCGGTGGTGCTGTCGGCCATCC
TGACCGTGGTCTGCCTGGCCGGCCTGGCCGCCCTGTACCTGCGCGATTCGCGCGTGCGTACGATGGAAGCAGAAGCGCGC
CAGCGCGAAGCTGAAGAGCGCCGGATGGTGGAAAAGCGCAACAACGATTCCACCCAGGCCGCAATTCTGCGTCTGATGAA
CGAACTGCAGGACATTGCAGACGGTGACTTGACCAAGCAGGCCACCGTGTCGGAAGACATTACAGGCGCCATCGCCGACT
CGGTGAACTACACCGTGGAAGAACTGCGCGAGCTGGTCGGCCGGGTGCAGCAGACGGCCGAGCAGGTGACGCAGGCATCG
TCGGCGGTGCAGACCACGTCGGAAAGCCTGGTGGCCGCATCGGAAGAACAATCGCGCCAGATTCGTCAGACCGGTCAGTC
GGTGGTGGAGATGGCCGACCGCATTACGCAGGTCTCGCGCGGTGCTGCTGAATCGGCCAACGTTGCACGCGCCTCGCTGG
CCGCTGCCGAGCAAGGTCAGCAGGCGGTGCAGAACGCCATCACGGGCATGAACGACATCCGTGACCAGATTCAGGAAACC
TCGAAGCGGATCAAGCGCCTGGGCGAATCGTCGCAGGAGATCGGTGAAATCGTGGAGCTGATCTCGGACATTACCGAGCA
GACCAACGTGCTGGCACTGAACGCTGCCATTCAGGCGGCATCCGCTGGTGAAGCCGGTCGCGGCTTCTCGGTGGTGGCAG
AAGAAGTGCAGCGCCTGGCAGAACGTTCGGCCGAGGCAGCCAAGCAGATCGGCGCGCTGATCCGCACGATTCAGACCGAC
ACGCAAGACGCGGTGCACGCCATGGAGCGCAGCACGGCGGGTGTGGTGGAAGGGGCGAAGCTGTCCGATAACGCCGGTGC
TGCACTGGTGGAGATCGGTCGCGTGTCCCGCCAGCTTGCCGAACTGATTGAGAGCATTTCGCAGACGACGTCGCACGAAG
CGACGCTGGCTTCGGACGTTGCGCAGAACATCGAACAGATCCTGCACATCACGGAACAGACGTCGACCGGTACGCGCCAG
ACCGCGCAGTCGGTGCGCCAGCTCACGCAGCTTGCCGAAGAACTGCGTGACTCGGTGGCGCGGTTCCGCATCGCCTGA

Upstream 100 bases:

>100_bases
GTACCACGCCAGGTTGAACAAGGCGCGGACGCGGACGGGAAATCCATGCGGCTGATCTCGACAGAACGAGACGGCAGCAT
CGACAAGACAAGAGGGTCCT

Downstream 100 bases:

>100_bases
TCTCGTCACCTCTGGATTTCGGGCGGGAACGCCGAAGGGGCCACCCCGGGGGCAATCGCGGCAACCGCCACCTGCTTTTT
GGAAGCACACATGCGTCATC

Product: methyl-accepting chemotaxis sensory transducer

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 745; Mature: 744

Protein sequence:

>745_residues
MGFKWFNAGRRAGEAGAPEVAAEGASAASVQLAQLDNASHTDDDNPAGTPTQLGRSPMDAVVGWMERVPFAAQQRVFTIG
LVVGLLALLISVYLDNRQANNGSVQIEIAGDTLMHSQRLAKAVPVALLGNTNAFAQLKESRARLQENLDALKNGNSERGV
RASGGEAEALLDKSIEEWKRSDKSAQAILAQEKTLVAVGKTLNVFNASNPELLEEAEQISSMKLQTNAPAREVAASSQLV
MLTQRLGKNMNEFLAGEGVNPETAFLLGKDTNTFRETLNGLLNGSEALRLSAATDAETKGYLQELSTRFDNVQKTTQIIL
ENLPGLIAAKRAQQQIFNDNEKLRADLSDLQSAYAGSVRSRPMTLGAVVLSAILTVVCLAGLAALYLRDSRVRTMEAEAR
QREAEERRMVEKRNNDSTQAAILRLMNELQDIADGDLTKQATVSEDITGAIADSVNYTVEELRELVGRVQQTAEQVTQAS
SAVQTTSESLVAASEEQSRQIRQTGQSVVEMADRITQVSRGAAESANVARASLAAAEQGQQAVQNAITGMNDIRDQIQET
SKRIKRLGESSQEIGEIVELISDITEQTNVLALNAAIQAASAGEAGRGFSVVAEEVQRLAERSAEAAKQIGALIRTIQTD
TQDAVHAMERSTAGVVEGAKLSDNAGAALVEIGRVSRQLAELIESISQTTSHEATLASDVAQNIEQILHITEQTSTGTRQ
TAQSVRQLTQLAEELRDSVARFRIA

Sequences:

>Translated_745_residues
MGFKWFNAGRRAGEAGAPEVAAEGASAASVQLAQLDNASHTDDDNPAGTPTQLGRSPMDAVVGWMERVPFAAQQRVFTIG
LVVGLLALLISVYLDNRQANNGSVQIEIAGDTLMHSQRLAKAVPVALLGNTNAFAQLKESRARLQENLDALKNGNSERGV
RASGGEAEALLDKSIEEWKRSDKSAQAILAQEKTLVAVGKTLNVFNASNPELLEEAEQISSMKLQTNAPAREVAASSQLV
MLTQRLGKNMNEFLAGEGVNPETAFLLGKDTNTFRETLNGLLNGSEALRLSAATDAETKGYLQELSTRFDNVQKTTQIIL
ENLPGLIAAKRAQQQIFNDNEKLRADLSDLQSAYAGSVRSRPMTLGAVVLSAILTVVCLAGLAALYLRDSRVRTMEAEAR
QREAEERRMVEKRNNDSTQAAILRLMNELQDIADGDLTKQATVSEDITGAIADSVNYTVEELRELVGRVQQTAEQVTQAS
SAVQTTSESLVAASEEQSRQIRQTGQSVVEMADRITQVSRGAAESANVARASLAAAEQGQQAVQNAITGMNDIRDQIQET
SKRIKRLGESSQEIGEIVELISDITEQTNVLALNAAIQAASAGEAGRGFSVVAEEVQRLAERSAEAAKQIGALIRTIQTD
TQDAVHAMERSTAGVVEGAKLSDNAGAALVEIGRVSRQLAELIESISQTTSHEATLASDVAQNIEQILHITEQTSTGTRQ
TAQSVRQLTQLAEELRDSVARFRIA
>Mature_744_residues
GFKWFNAGRRAGEAGAPEVAAEGASAASVQLAQLDNASHTDDDNPAGTPTQLGRSPMDAVVGWMERVPFAAQQRVFTIGL
VVGLLALLISVYLDNRQANNGSVQIEIAGDTLMHSQRLAKAVPVALLGNTNAFAQLKESRARLQENLDALKNGNSERGVR
ASGGEAEALLDKSIEEWKRSDKSAQAILAQEKTLVAVGKTLNVFNASNPELLEEAEQISSMKLQTNAPAREVAASSQLVM
LTQRLGKNMNEFLAGEGVNPETAFLLGKDTNTFRETLNGLLNGSEALRLSAATDAETKGYLQELSTRFDNVQKTTQIILE
NLPGLIAAKRAQQQIFNDNEKLRADLSDLQSAYAGSVRSRPMTLGAVVLSAILTVVCLAGLAALYLRDSRVRTMEAEARQ
REAEERRMVEKRNNDSTQAAILRLMNELQDIADGDLTKQATVSEDITGAIADSVNYTVEELRELVGRVQQTAEQVTQASS
AVQTTSESLVAASEEQSRQIRQTGQSVVEMADRITQVSRGAAESANVARASLAAAEQGQQAVQNAITGMNDIRDQIQETS
KRIKRLGESSQEIGEIVELISDITEQTNVLALNAAIQAASAGEAGRGFSVVAEEVQRLAERSAEAAKQIGALIRTIQTDT
QDAVHAMERSTAGVVEGAKLSDNAGAALVEIGRVSRQLAELIESISQTTSHEATLASDVAQNIEQILHITEQTSTGTRQT
AQSVRQLTQLAEELRDSVARFRIA

Specific function: May be a part of a signal-transduction system that regulates twitching motility by controlling pilus function (extension and retraction) [H]

COG id: COG0840

COG function: function code NT; Methyl-accepting chemotaxis protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI2367378, Length=326, Percent_Identity=30.0613496932515, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1788194, Length=320, Percent_Identity=29.375, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788195, Length=325, Percent_Identity=27.6923076923077, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1789453, Length=299, Percent_Identity=30.4347826086957, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1787690, Length=282, Percent_Identity=31.9148936170213, Blast_Score=99, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 80006; Mature: 79874

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFKWFNAGRRAGEAGAPEVAAEGASAASVQLAQLDNASHTDDDNPAGTPTQLGRSPMDA
CCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCHHHH
VVGWMERVPFAAQQRVFTIGLVVGLLALLISVYLDNRQANNGSVQIEIAGDTLMHSQRLA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHH
KAVPVALLGNTNAFAQLKESRARLQENLDALKNGNSERGVRASGGEAEALLDKSIEEWKR
HHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
SDKSAQAILAQEKTLVAVGKTLNVFNASNPELLEEAEQISSMKLQTNAPAREVAASSQLV
CCHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHH
MLTQRLGKNMNEFLAGEGVNPETAFLLGKDTNTFRETLNGLLNGSEALRLSAATDAETKG
HHHHHHCCCHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCHHEEEECCCCCHHHH
YLQELSTRFDNVQKTTQIILENLPGLIAAKRAQQQIFNDNEKLRADLSDLQSAYAGSVRS
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
RPMTLGAVVLSAILTVVCLAGLAALYLRDSRVRTMEAEARQREAEERRMVEKRNNDSTQA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AILRLMNELQDIADGDLTKQATVSEDITGAIADSVNYTVEELRELVGRVQQTAEQVTQAS
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAVQTTSESLVAASEEQSRQIRQTGQSVVEMADRITQVSRGAAESANVARASLAAAEQGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
QAVQNAITGMNDIRDQIQETSKRIKRLGESSQEIGEIVELISDITEQTNVLALNAAIQAA
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCEEEHHHHHHHH
SAGEAGRGFSVVAEEVQRLAERSAEAAKQIGALIRTIQTDTQDAVHAMERSTAGVVEGAK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
LSDNAGAALVEIGRVSRQLAELIESISQTTSHEATLASDVAQNIEQILHITEQTSTGTRQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
TAQSVRQLTQLAEELRDSVARFRIA
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GFKWFNAGRRAGEAGAPEVAAEGASAASVQLAQLDNASHTDDDNPAGTPTQLGRSPMDA
CCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCHHHH
VVGWMERVPFAAQQRVFTIGLVVGLLALLISVYLDNRQANNGSVQIEIAGDTLMHSQRLA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHH
KAVPVALLGNTNAFAQLKESRARLQENLDALKNGNSERGVRASGGEAEALLDKSIEEWKR
HHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
SDKSAQAILAQEKTLVAVGKTLNVFNASNPELLEEAEQISSMKLQTNAPAREVAASSQLV
CCHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHH
MLTQRLGKNMNEFLAGEGVNPETAFLLGKDTNTFRETLNGLLNGSEALRLSAATDAETKG
HHHHHHCCCHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCHHEEEECCCCCHHHH
YLQELSTRFDNVQKTTQIILENLPGLIAAKRAQQQIFNDNEKLRADLSDLQSAYAGSVRS
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
RPMTLGAVVLSAILTVVCLAGLAALYLRDSRVRTMEAEARQREAEERRMVEKRNNDSTQA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AILRLMNELQDIADGDLTKQATVSEDITGAIADSVNYTVEELRELVGRVQQTAEQVTQAS
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAVQTTSESLVAASEEQSRQIRQTGQSVVEMADRITQVSRGAAESANVARASLAAAEQGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
QAVQNAITGMNDIRDQIQETSKRIKRLGESSQEIGEIVELISDITEQTNVLALNAAIQAA
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCEEEHHHHHHHH
SAGEAGRGFSVVAEEVQRLAERSAEAAKQIGALIRTIQTDTQDAVHAMERSTAGVVEGAK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
LSDNAGAALVEIGRVSRQLAELIESISQTTSHEATLASDVAQNIEQILHITEQTSTGTRQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
TAQSVRQLTQLAEELRDSVARFRIA
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7908398; 10984043 [H]