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The map label for this gene is estA [H]

Identifier: 187927598

GI number: 187927598

Start: 530665

End: 531708

Strand: Direct

Name: estA [H]

Synonym: Rpic_0498

Alternate gene names: 187927598

Gene position: 530665-531708 (Clockwise)

Preceding gene: 187927597

Following gene: 187927603

Centisome position: 13.46

GC content: 64.08

Gene sequence:

>1044_bases
ATGAAGTTCCGTCAATGGATGGGCGTCGTCGCATCGGCGACCCTGGCCTTGAGCCTGGCCGCTTGCGGCGGCGAAAGCGA
TCAGAGCGGCAATCCCAACGTCGCCAAAGTGCAGCGCATGGTGGTGTTCGGCGACAGCCTGAGCGATGCCGGCACCTACA
CGCCGGCAGCGGCTGCCGTGGGCGGCGGCAAGTTCACTACCAACCCTGGCCCGGTCTGGGCGGAAACGGTGGCGGCGCAA
CTCGGTGTGGCCCTTACGCCGGCCGTGATGGGTTATGCCAACTCGGTGCAGAACTGCCCAAAACCTGGTTGCTTCGACTA
CGCGCAAGGCGGTTCGCGCGTGACCGACCCGAACGGTATCGGCCACAACGGTGGCGCCGGGGCGCTCACGTATCCGGTCA
AGCAGCAACTCGCCAACTTCTACGCGGCCAGCAACAACACGTTCAACGGCAACAACGACGTGGTGTTCGTCTTTGGCGGC
AACAACGACATCTTCTATTGGGCCGCCGCCGTGGCGACCCCCAATTCGGGTGTGACGCCTGCCATCGCCACCGCTCAAGT
TCAGCAGGCGGCGACTGATCTGGTCGGTTACGTCAAGGACATGGTCAGCAAGAACGCAACGCAGCTTTACGTGTTCAACC
TGCCCGACAGCAGCCTGACGCCGCAAGGCGTGGCGGGCGGTGCGGCAGGTCAGGCGCTGCTGCATGCGCTGGTGGGCACG
TTCAACACCACGCTGCAGGCTGGGCTTGCCGGTACCGGTGCCCGCGTGATCGACTTCAATTCGCAATTGACGGCCGTGAT
TCAGAACGGGGCGATGTTCGGCTTCAGCAATACCAGCGTGCCGGCTTGCGATGCGACCAAGATCAACGCACTCGTGCCGG
GTGCTGGCGGCAGCTCGCTGTTCTGCTCCGCCAACACGTTGGTGACTGCAGGTGCAGACACGGCGTACCTGTTCGCCGAC
GGCGTGCACCCCACCACAGGTGGCCATCGCCTGATCTCCAGCAACGTGCTGCAACGCCTGCTGGCCGACGGTGTAACGCA
CTGA

Upstream 100 bases:

>100_bases
ACTTGGGCGTTGTGTCGTTCCGCGTGCTTCAAACACGCACGGCGAGGTTACGCACCGCGGCACCACAGACCATAAAAATT
CCACACGGGAGACAGACATC

Downstream 100 bases:

>100_bases
CCAAGCACCACTGAGCAAAATAACGCCCGCGCGAAGCGGGCGTTCTTGTTTGCGGCGATGGTTTCTATTTGGCGTCGGCC
GCTGGCAAGGTCACGCCAAG

Product: G-D-S-L family lipolytic protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKFRQWMGVVASATLALSLAACGGESDQSGNPNVAKVQRMVVFGDSLSDAGTYTPAAAAVGGGKFTTNPGPVWAETVAAQ
LGVALTPAVMGYANSVQNCPKPGCFDYAQGGSRVTDPNGIGHNGGAGALTYPVKQQLANFYAASNNTFNGNNDVVFVFGG
NNDIFYWAAAVATPNSGVTPAIATAQVQQAATDLVGYVKDMVSKNATQLYVFNLPDSSLTPQGVAGGAAGQALLHALVGT
FNTTLQAGLAGTGARVIDFNSQLTAVIQNGAMFGFSNTSVPACDATKINALVPGAGGSSLFCSANTLVTAGADTAYLFAD
GVHPTTGGHRLISSNVLQRLLADGVTH

Sequences:

>Translated_347_residues
MKFRQWMGVVASATLALSLAACGGESDQSGNPNVAKVQRMVVFGDSLSDAGTYTPAAAAVGGGKFTTNPGPVWAETVAAQ
LGVALTPAVMGYANSVQNCPKPGCFDYAQGGSRVTDPNGIGHNGGAGALTYPVKQQLANFYAASNNTFNGNNDVVFVFGG
NNDIFYWAAAVATPNSGVTPAIATAQVQQAATDLVGYVKDMVSKNATQLYVFNLPDSSLTPQGVAGGAAGQALLHALVGT
FNTTLQAGLAGTGARVIDFNSQLTAVIQNGAMFGFSNTSVPACDATKINALVPGAGGSSLFCSANTLVTAGADTAYLFAD
GVHPTTGGHRLISSNVLQRLLADGVTH
>Mature_347_residues
MKFRQWMGVVASATLALSLAACGGESDQSGNPNVAKVQRMVVFGDSLSDAGTYTPAAAAVGGGKFTTNPGPVWAETVAAQ
LGVALTPAVMGYANSVQNCPKPGCFDYAQGGSRVTDPNGIGHNGGAGALTYPVKQQLANFYAASNNTFNGNNDVVFVFGG
NNDIFYWAAAVATPNSGVTPAIATAQVQQAATDLVGYVKDMVSKNATQLYVFNLPDSSLTPQGVAGGAAGQALLHALVGT
FNTTLQAGLAGTGARVIDFNSQLTAVIQNGAMFGFSNTSVPACDATKINALVPGAGGSSLFCSANTLVTAGADTAYLFAD
GVHPTTGGHRLISSNVLQRLLADGVTH

Specific function: Unknown

COG id: COG3240

COG function: function code IR; Phospholipase/lecithinase/hemolysin

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 autotransporter (TC 1.B.12) domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005546
- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR017186
- InterPro:   IPR001087
- InterPro:   IPR008265
- InterPro:   IPR006315 [H]

Pfam domain/function: PF03797 Autotransporter; PF00657 Lipase_GDSL [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 35135; Mature: 35135

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01098 LIPASE_GDSL_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFRQWMGVVASATLALSLAACGGESDQSGNPNVAKVQRMVVFGDSLSDAGTYTPAAAAV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEEEEEECCCCCCCCCCCCHHHHC
GGGKFTTNPGPVWAETVAAQLGVALTPAVMGYANSVQNCPKPGCFDYAQGGSRVTDPNGI
CCCCEECCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCC
GHNGGAGALTYPVKQQLANFYAASNNTFNGNNDVVFVFGGNNDIFYWAAAVATPNSGVTP
CCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEEEEEECCCCCCCC
AIATAQVQQAATDLVGYVKDMVSKNATQLYVFNLPDSSLTPQGVAGGAAGQALLHALVGT
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
FNTTLQAGLAGTGARVIDFNSQLTAVIQNGAMFGFSNTSVPACDATKINALVPGAGGSSL
HHHHHHHCCCCCCCEEEECCCCEEEEECCCEEEECCCCCCCCCCCCCCEEEECCCCCCEE
FCSANTLVTAGADTAYLFADGVHPTTGGHRLISSNVLQRLLADGVTH
EEECCEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKFRQWMGVVASATLALSLAACGGESDQSGNPNVAKVQRMVVFGDSLSDAGTYTPAAAAV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEEEEEECCCCCCCCCCCCHHHHC
GGGKFTTNPGPVWAETVAAQLGVALTPAVMGYANSVQNCPKPGCFDYAQGGSRVTDPNGI
CCCCEECCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCC
GHNGGAGALTYPVKQQLANFYAASNNTFNGNNDVVFVFGGNNDIFYWAAAVATPNSGVTP
CCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEEEEEECCCCCCCC
AIATAQVQQAATDLVGYVKDMVSKNATQLYVFNLPDSSLTPQGVAGGAAGQALLHALVGT
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
FNTTLQAGLAGTGARVIDFNSQLTAVIQNGAMFGFSNTSVPACDATKINALVPGAGGSSL
HHHHHHHCCCCCCCEEEECCCCEEEEECCCEEEECCCCCCCCCCCCCCEEEECCCCCCEE
FCSANTLVTAGADTAYLFADGVHPTTGGHRLISSNVLQRLLADGVTH
EEECCEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10559163; 10984043 [H]