Click here to switch to the map view.

The map label for this gene is yfnC [H]

Identifier: 187935240

GI number: 187935240

Start: 1977053

End: 1978291

Strand: Reverse

Name: yfnC [H]

Synonym: CLL_A1875

Alternate gene names: 187935240

Gene position: 1978291-1977053 (Counterclockwise)

Preceding gene: 187935581

Following gene: 187932926

Centisome position: 52.06

GC content: 31.64

Gene sequence:

>1239_bases
ATGAACAAAAAATTTATAAGACTATTTGCAGTTGGACATTTAGTAACAGACATTTATCAAGGAGCATTACCAGCAATGCT
ACCATTTCTTATATCAGAGAAAAACTTAAGTTATGCAGCAGCAGCATTCTTAATTTTTGCAGCCAATGCGAGCTCATCAA
TAATACAACCTCTATTTGGAATTTATGCAGATAAAATTTCGCTTCCGTGGGCACTTGGAACGGGTGTATTACTAGGTGGA
ATAGGAATTGGTGTATCAGGAGTTACAAGTAGTTATAATATGATATTTGCGTTAGTAGCTTTAAGTGGAGTAGGAATAGC
ATTATATCATCCAGAAGGTGCTAGGCTTACAAATAAATTTTCAGGTAAAAATAAAACAACTGCTGTGAGTAAATTTGCAG
CAGGCGGAAATATTGGTTTTGCAGTAGGACCAATAATAACAACAGTAATTTTGTCAATCTTGGGTTTAAAGGGAACTATA
GTATTGTGTATTCCTGCAATAATAATGGGATTAATTCTTTTATATGAAGCAAGATATTTTTCTAAAAATGAAAATGAATT
AGTTAATGAGAGAGATTGTATAAGGAATAAAGGAGAAGTAAAAGCAGATCAGTGGGGTGCTTTTGGAAGATTAACACTTA
CACTTCTTTGTAGATCTACAGTATTTTTTGGACTTAATACATTTTTAGCACTTTATTTTGTACATGTATTGAATCAATCA
GAAGTACATGGAAGTATAGCGTTATCTACATTAATTGTTGTTGGAGCAGTTGGAACATTATTTTCAGGAAAACTTGCTGA
TAAGGTTGGAAATAAGAAGGTAATAATAGTGGGTTATTCTTGTTTAGTACCATTATTAATATGTTTTTTAAGTATAAAAA
ATCCAATAATAGTTACAATAATACTGATTCCATTAGGATTTTTCTTATATATGCCCTATAGCCCAATGATAGCACTTGGA
CAAAAGTACTTGCCTAATCATGTTGGACTAGCTTCAGGGGTTACAATGGGATTAGGAGTTACTATGGGTGGTGTTGTATC
ACCAATACTTGGATGGGTTTCAGATAACTATGGTATACACACAGCATTATGTACTTTAATGGTAATGCCTGTTTTTGCAG
TGATTTTGGCAAGAAAATTACCTGTATATAATGAAGATATAGAAAATGATGAAATACAAAAAGATGTACAATCAAACACT
AAAAAGTTATCAAATAAGAGTTTAAATTTACAAAAATAA

Upstream 100 bases:

>100_bases
TCTTGATATAATAATAAAGAATAAATTATATTTTTACAAGTAAAGATAAATAATACAAAATTACTTAAAAATATAAACTT
ATATGAAAAGAAGGTTTAAT

Downstream 100 bases:

>100_bases
TAAAATTTTTAAATAATATAAAGAATATGAGTTAAATTAATTGAGACTATATAATAAAAAATGTTAAATAATAAAATTAA
AATTTTTATGCCTATTATCT

Product: major facilitator superfamily MFS_1

Products: fosmidomycin [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MNKKFIRLFAVGHLVTDIYQGALPAMLPFLISEKNLSYAAAAFLIFAANASSSIIQPLFGIYADKISLPWALGTGVLLGG
IGIGVSGVTSSYNMIFALVALSGVGIALYHPEGARLTNKFSGKNKTTAVSKFAAGGNIGFAVGPIITTVILSILGLKGTI
VLCIPAIIMGLILLYEARYFSKNENELVNERDCIRNKGEVKADQWGAFGRLTLTLLCRSTVFFGLNTFLALYFVHVLNQS
EVHGSIALSTLIVVGAVGTLFSGKLADKVGNKKVIIVGYSCLVPLLICFLSIKNPIIVTIILIPLGFFLYMPYSPMIALG
QKYLPNHVGLASGVTMGLGVTMGGVVSPILGWVSDNYGIHTALCTLMVMPVFAVILARKLPVYNEDIENDEIQKDVQSNT
KKLSNKSLNLQK

Sequences:

>Translated_412_residues
MNKKFIRLFAVGHLVTDIYQGALPAMLPFLISEKNLSYAAAAFLIFAANASSSIIQPLFGIYADKISLPWALGTGVLLGG
IGIGVSGVTSSYNMIFALVALSGVGIALYHPEGARLTNKFSGKNKTTAVSKFAAGGNIGFAVGPIITTVILSILGLKGTI
VLCIPAIIMGLILLYEARYFSKNENELVNERDCIRNKGEVKADQWGAFGRLTLTLLCRSTVFFGLNTFLALYFVHVLNQS
EVHGSIALSTLIVVGAVGTLFSGKLADKVGNKKVIIVGYSCLVPLLICFLSIKNPIIVTIILIPLGFFLYMPYSPMIALG
QKYLPNHVGLASGVTMGLGVTMGGVVSPILGWVSDNYGIHTALCTLMVMPVFAVILARKLPVYNEDIENDEIQKDVQSNT
KKLSNKSLNLQK
>Mature_412_residues
MNKKFIRLFAVGHLVTDIYQGALPAMLPFLISEKNLSYAAAAFLIFAANASSSIIQPLFGIYADKISLPWALGTGVLLGG
IGIGVSGVTSSYNMIFALVALSGVGIALYHPEGARLTNKFSGKNKTTAVSKFAAGGNIGFAVGPIITTVILSILGLKGTI
VLCIPAIIMGLILLYEARYFSKNENELVNERDCIRNKGEVKADQWGAFGRLTLTLLCRSTVFFGLNTFLALYFVHVLNQS
EVHGSIALSTLIVVGAVGTLFSGKLADKVGNKKVIIVGYSCLVPLLICFLSIKNPIIVTIILIPLGFFLYMPYSPMIALG
QKYLPNHVGLASGVTMGLGVTMGGVVSPILGWVSDNYGIHTALCTLMVMPVFAVILARKLPVYNEDIENDEIQKDVQSNT
KKLSNKSLNLQK

Specific function: Confers The Resistance Against Fosmidomycin. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786686, Length=364, Percent_Identity=26.9230769230769, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44142; Mature: 44142

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKFIRLFAVGHLVTDIYQGALPAMLPFLISEKNLSYAAAAFLIFAANASSSIIQPLFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
IYADKISLPWALGTGVLLGGIGIGVSGVTSSYNMIFALVALSGVGIALYHPEGARLTNKF
HHHHHCCCCHHHHHHHHHHHHHCCCHHHCHHHHHHHHHHHHHCCCEEEECCCCCCHHCCC
SGKNKTTAVSKFAAGGNIGFAVGPIITTVILSILGLKGTIVLCIPAIIMGLILLYEARYF
CCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
SKNENELVNERDCIRNKGEVKADQWGAFGRLTLTLLCRSTVFFGLNTFLALYFVHVLNQS
CCCCHHHCCHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EVHGSIALSTLIVVGAVGTLFSGKLADKVGNKKVIIVGYSCLVPLLICFLSIKNPIIVTI
HHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEEEEHHHHHHHHHHHHHCCCCHHHHH
ILIPLGFFLYMPYSPMIALGQKYLPNHVGLASGVTMGLGVTMGGVVSPILGWVSDNYGIH
HHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHH
TALCTLMVMPVFAVILARKLPVYNEDIENDEIQKDVQSNTKKLSNKSLNLQK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNKKFIRLFAVGHLVTDIYQGALPAMLPFLISEKNLSYAAAAFLIFAANASSSIIQPLFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
IYADKISLPWALGTGVLLGGIGIGVSGVTSSYNMIFALVALSGVGIALYHPEGARLTNKF
HHHHHCCCCHHHHHHHHHHHHHCCCHHHCHHHHHHHHHHHHHCCCEEEECCCCCCHHCCC
SGKNKTTAVSKFAAGGNIGFAVGPIITTVILSILGLKGTIVLCIPAIIMGLILLYEARYF
CCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
SKNENELVNERDCIRNKGEVKADQWGAFGRLTLTLLCRSTVFFGLNTFLALYFVHVLNQS
CCCCHHHCCHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EVHGSIALSTLIVVGAVGTLFSGKLADKVGNKKVIIVGYSCLVPLLICFLSIKNPIIVTI
HHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEEEEHHHHHHHHHHHHHCCCCHHHHH
ILIPLGFFLYMPYSPMIALGQKYLPNHVGLASGVTMGLGVTMGGVVSPILGWVSDNYGIH
HHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHH
TALCTLMVMPVFAVILARKLPVYNEDIENDEIQKDVQSNTKKLSNKSLNLQK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: fosmidomycin [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: fosmidomycin [Cytoplasm] + Proton [Periplasm] = fosmidomycin [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9141694; 9384377 [H]