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The map label for this gene is ispF [H]

Identifier: 187934964

GI number: 187934964

Start: 357965

End: 358438

Strand: Reverse

Name: ispF [H]

Synonym: CLL_A0353

Alternate gene names: 187934964

Gene position: 358438-357965 (Counterclockwise)

Preceding gene: 187933622

Following gene: 187935189

Centisome position: 9.43

GC content: 28.06

Gene sequence:

>474_bases
ATGAGAGTTGGACTTGGATATGATGTTCATAAACTAGTTGAAAATAGAAAATTAATTTTAGGTGGAGTTGAAATTCCTTA
TGAAAAAGGATTATTAGGACATTCTGATGCTGATGTTTTATTACATGCAATAATGGATTCATTATTAGGAGCTTGTGCAT
TAGGGGACATTGGTCGTCACTTTCCTGATACTGATAATAAATTTAAAGGAATATCTAGTATAAAACTTTTAGAGGAAGTA
AATAAATTAATCATAAAAAATAGATATATTATAAATAATATTGATTCTGTTATTATTGCTCAAAAACCAAAACTTGCTCC
ATATATAGACACTATGACAGAAAATATATCTAATGCTCTTAATATAAAGATTGATCAAATTAATATAAAAGCTACTACAG
AAGAGGGGCTTGGCTTCACAGGAGGTATGCTTGGAATATCATCACAAAGCATATGTTCTGTTATTAAAAAATAA

Upstream 100 bases:

>100_bases
AATGATAATAATAATACTAAAATTGATTACTATAAATTTAAATGATAAAATTAAAATATGTTTATTAAAATTTATTTTAA
CTTGAAAGGAACTTTGTTAT

Downstream 100 bases:

>100_bases
TATTTTAAGGGTTACTAGAAAATTTTTTAAAATAAATTCTAGTAACCCTTATTAATTTTTATAAAGCATGGCCTTATTTT
AAAATACTTCTGTCAATCTT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MRVGLGYDVHKLVENRKLILGGVEIPYEKGLLGHSDADVLLHAIMDSLLGACALGDIGRHFPDTDNKFKGISSIKLLEEV
NKLIIKNRYIINNIDSVIIAQKPKLAPYIDTMTENISNALNIKIDQINIKATTEEGLGFTGGMLGISSQSICSVIKK

Sequences:

>Translated_157_residues
MRVGLGYDVHKLVENRKLILGGVEIPYEKGLLGHSDADVLLHAIMDSLLGACALGDIGRHFPDTDNKFKGISSIKLLEEV
NKLIIKNRYIINNIDSVIIAQKPKLAPYIDTMTENISNALNIKIDQINIKATTEEGLGFTGGMLGISSQSICSVIKK
>Mature_157_residues
MRVGLGYDVHKLVENRKLILGGVEIPYEKGLLGHSDADVLLHAIMDSLLGACALGDIGRHFPDTDNKFKGISSIKLLEEV
NKLIIKNRYIINNIDSVIIAQKPKLAPYIDTMTENISNALNIKIDQINIKATTEEGLGFTGGMLGISSQSICSVIKK

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=51.9230769230769, Blast_Score=155, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 17130; Mature: 17130

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVGLGYDVHKLVENRKLILGGVEIPYEKGLLGHSDADVLLHAIMDSLLGACALGDIGRH
CCCCCCCHHHHHHCCCEEEEECCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
FPDTDNKFKGISSIKLLEEVNKLIIKNRYIINNIDSVIIAQKPKLAPYIDTMTENISNAL
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCEEEECCCCCCHHHHHHHHHHCHHE
NIKIDQINIKATTEEGLGFTGGMLGISSQSICSVIKK
EEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MRVGLGYDVHKLVENRKLILGGVEIPYEKGLLGHSDADVLLHAIMDSLLGACALGDIGRH
CCCCCCCHHHHHHCCCEEEEECCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
FPDTDNKFKGISSIKLLEEVNKLIIKNRYIINNIDSVIIAQKPKLAPYIDTMTENISNAL
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCEEEECCCCCCHHHHHHHHHHCHHE
NIKIDQINIKATTEEGLGFTGGMLGISSQSICSVIKK
EEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA