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The map label for this gene is nirB [C]

Identifier: 187934833

GI number: 187934833

Start: 3048368

End: 3049042

Strand: Reverse

Name: nirB [C]

Synonym: CLL_A2962

Alternate gene names: 187934833

Gene position: 3049042-3048368 (Counterclockwise)

Preceding gene: 187933194

Following gene: 187935700

Centisome position: 80.23

GC content: 32.89

Gene sequence:

>675_bases
ATGAAAAAAGATTTATTAGACAAGGGCGCAATTTTACAAAGAGATAAGGAAACATATGCTATAGCACCTCATTTAACAGC
AGGGCTTATAACACCAGAACAATTAAGAACATTAGCTGATGTTGCAGAAAAATATGAGGTTAAAGCTGTAAAAGTAACAG
GAGCTCAACGTATTGCACTTGTTGGGATAAAAGAAGAAGAAATAGATAATGCATGGAAGGACTTGGATATGAAACCAGGA
GCTGCTGTTGGGTTATGTGTAAGAAGTGTTAAAATATGTCCAGGAACAACTTTTTGTAAAAAAGGACTACAAGATTCAGT
AGCAATTGGAAGTAAGCTTGATGGATTATACCATGGTAAAAATTTACCTAATAAGCTTAAAATAGGAGTTAGTGGATGTC
CACAAAGTTGTGCAGACAACCACATAAAAGATATCGGTATATATGGAATGCCTAAGGGATGGGTAGTTCAAATTGGTGGA
AAAGGTGGACTAAAACCAAGACTTGCAGATAAGATTGCAATGAATGTGTCAGAAGAAAAATTATTTCCATTAGTTGAAAA
AATAATTCAAATTTATTCAGAAAATGCTACTGCTAGAGAAAGATTAGGTGACTATATAGATAGAGTTGGATTAGAAGAAG
TTAAAAAACAAATAGATATTGAAAGTTATTTATAA

Upstream 100 bases:

>100_bases
CAATTTTAATAAATGAAAATTTAGTAATAGTTGGATTTAAAGAATATAATTATAGTAAATTACAATAATTATTTGTAGTA
TTATTAGGGAGGCATTAAAC

Downstream 100 bases:

>100_bases
TTTAAAATAGATCTAAAGTAAGAAATTAAGCAGAGAATTAGCAAAAGTAGCTATTTCTCTGCTTTAATGACTATCTTAAA
AAGTCTTTAAATGTTGCACG

Product: sulfite reductase, assimilatory-type

Products: 3 NAD(P)+; NH4OH; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MKKDLLDKGAILQRDKETYAIAPHLTAGLITPEQLRTLADVAEKYEVKAVKVTGAQRIALVGIKEEEIDNAWKDLDMKPG
AAVGLCVRSVKICPGTTFCKKGLQDSVAIGSKLDGLYHGKNLPNKLKIGVSGCPQSCADNHIKDIGIYGMPKGWVVQIGG
KGGLKPRLADKIAMNVSEEKLFPLVEKIIQIYSENATARERLGDYIDRVGLEEVKKQIDIESYL

Sequences:

>Translated_224_residues
MKKDLLDKGAILQRDKETYAIAPHLTAGLITPEQLRTLADVAEKYEVKAVKVTGAQRIALVGIKEEEIDNAWKDLDMKPG
AAVGLCVRSVKICPGTTFCKKGLQDSVAIGSKLDGLYHGKNLPNKLKIGVSGCPQSCADNHIKDIGIYGMPKGWVVQIGG
KGGLKPRLADKIAMNVSEEKLFPLVEKIIQIYSENATARERLGDYIDRVGLEEVKKQIDIESYL
>Mature_224_residues
MKKDLLDKGAILQRDKETYAIAPHLTAGLITPEQLRTLADVAEKYEVKAVKVTGAQRIALVGIKEEEIDNAWKDLDMKPG
AAVGLCVRSVKICPGTTFCKKGLQDSVAIGSKLDGLYHGKNLPNKLKIGVSGCPQSCADNHIKDIGIYGMPKGWVVQIGG
KGGLKPRLADKIAMNVSEEKLFPLVEKIIQIYSENATARERLGDYIDRVGLEEVKKQIDIESYL

Specific function: This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789765, Length=203, Percent_Identity=33.4975369458128, Blast_Score=137, Evalue=8e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR017220 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: 1.7.1.4 [C]

Molecular weight: Translated: 24487; Mature: 24487

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKDLLDKGAILQRDKETYAIAPHLTAGLITPEQLRTLADVAEKYEVKAVKVTGAQRIAL
CCCCHHCCCCHHCCCCCCEEECCCHHHCCCCHHHHHHHHHHHHHHHEEEEEECCCCEEEE
VGIKEEEIDNAWKDLDMKPGAAVGLCVRSVKICPGTTFCKKGLQDSVAIGSKLDGLYHGK
EECCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHCCHHCCC
NLPNKLKIGVSGCPQSCADNHIKDIGIYGMPKGWVVQIGGKGGLKPRLADKIAMNVSEEK
CCCCEEEECCCCCCHHHHHCCHHHCCCEECCCCCEEEECCCCCCCHHHHHHHHHCCCHHH
LFPLVEKIIQIYSENATARERLGDYIDRVGLEEVKKQIDIESYL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKDLLDKGAILQRDKETYAIAPHLTAGLITPEQLRTLADVAEKYEVKAVKVTGAQRIAL
CCCCHHCCCCHHCCCCCCEEECCCHHHCCCCHHHHHHHHHHHHHHHEEEEEECCCCEEEE
VGIKEEEIDNAWKDLDMKPGAAVGLCVRSVKICPGTTFCKKGLQDSVAIGSKLDGLYHGK
EECCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHCCHHCCC
NLPNKLKIGVSGCPQSCADNHIKDIGIYGMPKGWVVQIGGKGGLKPRLADKIAMNVSEEK
CCCCEEEECCCCCCHHHHHCCHHHCCCEECCCCCEEEECCCCCCCHHHHHHHHHCCCHHH
LFPLVEKIIQIYSENATARERLGDYIDRVGLEEVKKQIDIESYL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD; NAD+; NADH; Siroheme [C]

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.4 {NO2-}} 0.005 {NO2-}} 0.016 {NADH}} 5.3 {hydroxylamine}} [C]

Substrates: 3 NAD(P)H; nitrite [C]

Specific reaction: 3 NAD(P)H + nitrite = 3 NAD(P)+ + NH4OH + H2O [C]

General reaction: Redox reaction [C]

Inhibitor: 1, 10-Phenanthroline; 2, 2'-Bipyridine; Arsenite; CN-; NAD+; NADP+; NaN3; p-Chloromercuribenzoate; SO32- [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1911781 [H]