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The map label for this gene is cysL [H]

Identifier: 187934802

GI number: 187934802

Start: 2109063

End: 2109944

Strand: Reverse

Name: cysL [H]

Synonym: CLL_A2017

Alternate gene names: 187934802

Gene position: 2109944-2109063 (Counterclockwise)

Preceding gene: 187932792

Following gene: 187935022

Centisome position: 55.52

GC content: 29.37

Gene sequence:

>882_bases
ATGATAAGTAAATTAGATCTATATAAAGTTTTTCGTCAAGTGGGCAAAAGCAAGAGTTTTTCAAAGGCTGCAGCGGAACT
TTATATGACACAACCTGCTATAAGTCAGTCTATAATGCAGCTTGAAAGAGAATTAGACACTCGTCTTTTTAATAGAACAC
CTAAGGGCGTATCACTAACTAATGAAGGAAATCATCTCTTTGAATATGTAGATTCAGCTATAGGTTTAATAGAAGCTGGA
GAAGAAAAGATTTTAGAATTTCACAATTTATCTGTAGGAGAACTTAAAATTGGTGTGTGTGATACAATTTCAAAATATTA
TTTAATGCCTTATTTAGAGAAGTTTCATAATATGTATCCTAATATTAAGTTTAAGATCATTAATAGCACAACATTAGAGC
TGTGCACACTTATAAAAGGTGGTGAGATAGACATAGCAATATGTAATTTTCCAATAGATGACTCATCTTTAGAATTAAGA
CCATGTGTAGAAGTACAAGATATTTTCGTTTGTGGAGAGAAGTACAAGTATTTATTAAATGATAATATGTCACTTGACGA
TGTGGCTAATTTGCCATTAATATTTTTAGAACAAACATCAAATTCAAGAAAATATGTGGAAAATTTTATGGTTTCAAAAG
GGATAAGAATATCACCAGAGTTTGAACTTGGTTCATATGATTTGCTCTTAGAATTTGCAAAAATAAATCTAGGAATAGCA
TGTGTAGTTAAGGAATTTGCAAGGGAATATTTAAATAAAGGTTTAATATATGAAATAAAAACAGTAGAAGAGATTCCTAA
AAGAAATATAGGGGTATGTTATTTAAAAAGAGTTCCACTTTCATTAGCTTCAACTAAATTTGTAGAAATAATTGAAGAGT
AA

Upstream 100 bases:

>100_bases
AAAATAAATTCAAAATAATATGGTTTATACTTGTAATATACTCCAATTAATGATATAAGTAAAATAAATAGTAAATATAT
CTAATATAAGGAGAGCTTAT

Downstream 100 bases:

>100_bases
ATCTGTAAGTATTAAATATTATTACTTAATTTTAATAATGTTTTAAAATATAAATAAAAGTGATGTTACTTTACTTCACT
TTTATTTATTATATGTACTT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MISKLDLYKVFRQVGKSKSFSKAAAELYMTQPAISQSIMQLERELDTRLFNRTPKGVSLTNEGNHLFEYVDSAIGLIEAG
EEKILEFHNLSVGELKIGVCDTISKYYLMPYLEKFHNMYPNIKFKIINSTTLELCTLIKGGEIDIAICNFPIDDSSLELR
PCVEVQDIFVCGEKYKYLLNDNMSLDDVANLPLIFLEQTSNSRKYVENFMVSKGIRISPEFELGSYDLLLEFAKINLGIA
CVVKEFAREYLNKGLIYEIKTVEEIPKRNIGVCYLKRVPLSLASTKFVEIIEE

Sequences:

>Translated_293_residues
MISKLDLYKVFRQVGKSKSFSKAAAELYMTQPAISQSIMQLERELDTRLFNRTPKGVSLTNEGNHLFEYVDSAIGLIEAG
EEKILEFHNLSVGELKIGVCDTISKYYLMPYLEKFHNMYPNIKFKIINSTTLELCTLIKGGEIDIAICNFPIDDSSLELR
PCVEVQDIFVCGEKYKYLLNDNMSLDDVANLPLIFLEQTSNSRKYVENFMVSKGIRISPEFELGSYDLLLEFAKINLGIA
CVVKEFAREYLNKGLIYEIKTVEEIPKRNIGVCYLKRVPLSLASTKFVEIIEE
>Mature_293_residues
MISKLDLYKVFRQVGKSKSFSKAAAELYMTQPAISQSIMQLERELDTRLFNRTPKGVSLTNEGNHLFEYVDSAIGLIEAG
EEKILEFHNLSVGELKIGVCDTISKYYLMPYLEKFHNMYPNIKFKIINSTTLELCTLIKGGEIDIAICNFPIDDSSLELR
PCVEVQDIFVCGEKYKYLLNDNMSLDDVANLPLIFLEQTSNSRKYVENFMVSKGIRISPEFELGSYDLLLEFAKINLGIA
CVVKEFAREYLNKGLIYEIKTVEEIPKRNIGVCYLKRVPLSLASTKFVEIIEE

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=224, Percent_Identity=25.4464285714286, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1786401, Length=179, Percent_Identity=31.2849162011173, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI145693105, Length=294, Percent_Identity=24.8299319727891, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787601, Length=147, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788481, Length=283, Percent_Identity=22.9681978798587, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788748, Length=285, Percent_Identity=21.4035087719298, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787128, Length=250, Percent_Identity=27.6, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788296, Length=240, Percent_Identity=23.75, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787879, Length=291, Percent_Identity=22.3367697594502, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789639, Length=173, Percent_Identity=28.3236994219653, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786448, Length=135, Percent_Identity=30.3703703703704, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790262, Length=259, Percent_Identity=22.3938223938224, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787589, Length=117, Percent_Identity=29.0598290598291, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081904, Length=219, Percent_Identity=22.3744292237443, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788706, Length=269, Percent_Identity=25.2788104089219, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87082024, Length=179, Percent_Identity=24.5810055865922, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1789204, Length=251, Percent_Identity=22.3107569721116, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789173, Length=122, Percent_Identity=29.5081967213115, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1790208, Length=279, Percent_Identity=22.9390681003584, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33454; Mature: 33454

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISKLDLYKVFRQVGKSKSFSKAAAELYMTQPAISQSIMQLERELDTRLFNRTPKGVSLT
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEC
NEGNHLFEYVDSAIGLIEAGEEKILEFHNLSVGELKIGVCDTISKYYLMPYLEKFHNMYP
CCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCC
NIKFKIINSTTLELCTLIKGGEIDIAICNFPIDDSSLELRPCVEVQDIFVCGEKYKYLLN
CEEEEEECCCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHEEECCHHHEEEC
DNMSLDDVANLPLIFLEQTSNSRKYVENFMVSKGIRISPEFELGSYDLLLEFAKINLGIA
CCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHCCCHH
CVVKEFAREYLNKGLIYEIKTVEEIPKRNIGVCYLKRVPLSLASTKFVEIIEE
HHHHHHHHHHHCCCCEEEEHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHCC
>Mature Secondary Structure
MISKLDLYKVFRQVGKSKSFSKAAAELYMTQPAISQSIMQLERELDTRLFNRTPKGVSLT
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEC
NEGNHLFEYVDSAIGLIEAGEEKILEFHNLSVGELKIGVCDTISKYYLMPYLEKFHNMYP
CCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCC
NIKFKIINSTTLELCTLIKGGEIDIAICNFPIDDSSLELRPCVEVQDIFVCGEKYKYLLN
CEEEEEECCCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHEEECCHHHEEEC
DNMSLDDVANLPLIFLEQTSNSRKYVENFMVSKGIRISPEFELGSYDLLLEFAKINLGIA
CCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHCCCHH
CVVKEFAREYLNKGLIYEIKTVEEIPKRNIGVCYLKRVPLSLASTKFVEIIEE
HHHHHHHHHHHCCCCEEEEHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]