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The map label for this gene is cobJ [H]

Identifier: 187934491

GI number: 187934491

Start: 3012242

End: 3012979

Strand: Reverse

Name: cobJ [H]

Synonym: CLL_A2930

Alternate gene names: 187934491

Gene position: 3012979-3012242 (Counterclockwise)

Preceding gene: 187935212

Following gene: 187933316

Centisome position: 79.28

GC content: 30.35

Gene sequence:

>738_bases
TTGGGAGTACTTAGAGTTATAGGAATAGGTCCAGGTTCAATAGATAATATGAGCATAAGAGCTAAAAATGCTATTGAAAA
TAGTGACGTAATTGTAGGATATACAAAATACATAGATATGGTAAAAGATTTACTTAAGGATAAAGAAATTTATTCAACTG
GAATGATGGGTGAAGAAGCTAGATGTAAAGAGGCATTAGAACTTTCAAAAGATAAGAATGTAGCGTTAATAAGTACTGGG
GATTCAGGGATTTATGGAATGGCAGGATTAATTCTTGAGATGAGAACTAATGAAGAAGTAGAAATAATACCAGGAATAAC
AGCTTCAAGTTCTGCAGGATCAGTAGTTGGAGCACCTCTTATGCATGATAATTGTAATATAAGTTTAAGTGATTTAATGA
CACCTTATGAAGAGATTAAAAAAAGAGTTAGATTAGCGGCAGAAGGTGATTTTGTAATCTCTCTTTATAATCCTAGAAGT
AAGGGTAGAGATAAGTATTTAAAAGAATGTATTGATATAATAAGAGGATTTAGAAAAGATAATACTCCAATTGCAGTAGT
TAGAAATGCTTTAAGAGATGGTGAAAGTTATACATTAGCTGAGCTTAAAGACTTTGATTATGAAATTGTTGATATGATGT
CTATTGTTATTATAGGAAATTCAAAGAGTTTTCTTAAAAATGGATGTTTTATAACACCTAGAGGATATTCATCTAAGAAA
AGAGATACAGTAAAGTAA

Upstream 100 bases:

>100_bases
ATATCTAGATGGTGCAGAAATATTAATAAATAAGATAAAAGATAATGGAATTACATTATGCATTGGAATAAATAATGATT
AATTTTAGGAGGAAAATGAT

Downstream 100 bases:

>100_bases
TGATTGGTTTTATATTAGGAACTTCTGAAGGAAAAAATATTGTATCTTTAATTAATAAGTATACAAATGATATTGCAGTC
AGTACAGCAACAGCATATGG

Product: precorrin-3B C17-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MGVLRVIGIGPGSIDNMSIRAKNAIENSDVIVGYTKYIDMVKDLLKDKEIYSTGMMGEEARCKEALELSKDKNVALISTG
DSGIYGMAGLILEMRTNEEVEIIPGITASSSAGSVVGAPLMHDNCNISLSDLMTPYEEIKKRVRLAAEGDFVISLYNPRS
KGRDKYLKECIDIIRGFRKDNTPIAVVRNALRDGESYTLAELKDFDYEIVDMMSIVIIGNSKSFLKNGCFITPRGYSSKK
RDTVK

Sequences:

>Translated_245_residues
MGVLRVIGIGPGSIDNMSIRAKNAIENSDVIVGYTKYIDMVKDLLKDKEIYSTGMMGEEARCKEALELSKDKNVALISTG
DSGIYGMAGLILEMRTNEEVEIIPGITASSSAGSVVGAPLMHDNCNISLSDLMTPYEEIKKRVRLAAEGDFVISLYNPRS
KGRDKYLKECIDIIRGFRKDNTPIAVVRNALRDGESYTLAELKDFDYEIVDMMSIVIIGNSKSFLKNGCFITPRGYSSKK
RDTVK
>Mature_244_residues
GVLRVIGIGPGSIDNMSIRAKNAIENSDVIVGYTKYIDMVKDLLKDKEIYSTGMMGEEARCKEALELSKDKNVALISTGD
SGIYGMAGLILEMRTNEEVEIIPGITASSSAGSVVGAPLMHDNCNISLSDLMTPYEEIKKRVRLAAEGDFVISLYNPRSK
GRDKYLKECIDIIRGFRKDNTPIAVVRNALRDGESYTLAELKDFDYEIVDMMSIVIIGNSKSFLKNGCFITPRGYSSKKR
DTVK

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.131

Molecular weight: Translated: 27043; Mature: 26911

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVLRVIGIGPGSIDNMSIRAKNAIENSDVIVGYTKYIDMVKDLLKDKEIYSTGMMGEEA
CCEEEEEECCCCCCCCCEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHH
RCKEALELSKDKNVALISTGDSGIYGMAGLILEMRTNEEVEIIPGITASSSAGSVVGAPL
HHHHHHHHCCCCCEEEEECCCCCHHHHHEEEEEECCCCCEEEECCCCCCCCCCCEEECCE
MHDNCNISLSDLMTPYEEIKKRVRLAAEGDFVISLYNPRSKGRDKYLKECIDIIRGFRKD
EECCCCEEHHHHCCCHHHHHHHHEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCC
NTPIAVVRNALRDGESYTLAELKDFDYEIVDMMSIVIIGNSKSFLKNGCFITPRGYSSKK
CCHHHHHHHHHHCCCCEEEHHHHCCCHHHHHEEEEEEECCCHHHHHCCCEEECCCCCCCC
RDTVK
CCCCC
>Mature Secondary Structure 
GVLRVIGIGPGSIDNMSIRAKNAIENSDVIVGYTKYIDMVKDLLKDKEIYSTGMMGEEA
CEEEEEECCCCCCCCCEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHH
RCKEALELSKDKNVALISTGDSGIYGMAGLILEMRTNEEVEIIPGITASSSAGSVVGAPL
HHHHHHHHCCCCCEEEEECCCCCHHHHHEEEEEECCCCCEEEECCCCCCCCCCCEEECCE
MHDNCNISLSDLMTPYEEIKKRVRLAAEGDFVISLYNPRSKGRDKYLKECIDIIRGFRKD
EECCCCEEHHHHCCCHHHHHHHHEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCC
NTPIAVVRNALRDGESYTLAELKDFDYEIVDMMSIVIIGNSKSFLKNGCFITPRGYSSKK
CCHHHHHHHHHHCCCCEEEHHHHCCCHHHHHEEEEEEECCCHHHHHCCCEEECCCCCCCC
RDTVK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]