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The map label for this gene is ykfA [H]

Identifier: 187934289

GI number: 187934289

Start: 2560610

End: 2561527

Strand: Reverse

Name: ykfA [H]

Synonym: CLL_A2470

Alternate gene names: 187934289

Gene position: 2561527-2560610 (Counterclockwise)

Preceding gene: 187933376

Following gene: 187935293

Centisome position: 67.4

GC content: 29.41

Gene sequence:

>918_bases
TTGAGAAGACCTAAGCCATTAAAAAAAGGAGATAAAATAGCTATTGTAGGATTATCTAGTCCTACAACAGAAGAAAGATT
AGAATTATCTATTAAAGCAATGCATGATTTTGGATTTGAAGTTGTAGTTGGTGAGAGTTGCAAATCTCATTATGGTTATT
TAGCTGGAAGTGACGAGATAAGAGCAGATGATATGAATAAAATGTTTTGCGATAAATCAATATCTGGAATCTTTGCAATA
AGAGGAGGATATGGAGCAACAAGAATTTTAGATAAGCTCAATTACAATATGATAAAAAAGAATCCTAAAGTATTTTCAGG
ATATAGTGACGTTACAGCTATTCATAATGTAATAAATGCAAGATGCGATTTTATAACATTTCATGCACCGATGCCAGCAA
CAGAAATGTATAAGGGACTAGATAATTACACGGAATATTATTTTAAAAAGAGTATATTCGATTCAGAGCCATTAGGCAAA
TTAAAAAATACAGAAGATATAAAAATGAGAACTTTATTTAAAGGTAAAGCTGAAGGAAGATTAGTTGGTGGAAATCTTTC
AGTGATTTGCTCAACATTAGGAACTGAGTATGAAATAGATACTAAAGGAAAAATATTATTTTTAGAAGAGATTGATGAAT
ATCCATACAAAATTGATAGAATGATTATGCAATTAAAGCAGTCTAATAAATTTAAAGATGCAAGTGGAATAATTTTAGGA
AAATGGACTGATTGTTTACCACCAAAGGGAAGAGAAAGTTTATCATTAATTCAAATATTTGAAGAATTAATAGCGCCAGA
AAGAAAACCAACACTATATGATGTTTGTTGTGGCCATTGTTTACCAACACTTACTCTTCCTTTGGGTGCTAAAATAAAAA
TTAATGCAAATAGTAAAGAAATAAACATATTAGAATAA

Upstream 100 bases:

>100_bases
TGAAAAAAGGTGAAATAAGATTAATTTTAATACTTTAAGGATAAATAACTTTAAGTTTAAATATATTGATATTAAAGTAA
TTATATAGGATGGAAGTATA

Downstream 100 bases:

>100_bases
AAAATTTAAATATTAAATCAATAGACTATATTTTCCTTGGTATAACTTACTTATGGAATTGGAAAATATAAATTTGTATA
ATTATAAGTAAACTAATAAA

Product: muramoyltetrapeptide carboxypeptidase

Products: NA

Alternate protein names: LD-carboxypeptidase [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MRRPKPLKKGDKIAIVGLSSPTTEERLELSIKAMHDFGFEVVVGESCKSHYGYLAGSDEIRADDMNKMFCDKSISGIFAI
RGGYGATRILDKLNYNMIKKNPKVFSGYSDVTAIHNVINARCDFITFHAPMPATEMYKGLDNYTEYYFKKSIFDSEPLGK
LKNTEDIKMRTLFKGKAEGRLVGGNLSVICSTLGTEYEIDTKGKILFLEEIDEYPYKIDRMIMQLKQSNKFKDASGIILG
KWTDCLPPKGRESLSLIQIFEELIAPERKPTLYDVCCGHCLPTLTLPLGAKIKINANSKEINILE

Sequences:

>Translated_305_residues
MRRPKPLKKGDKIAIVGLSSPTTEERLELSIKAMHDFGFEVVVGESCKSHYGYLAGSDEIRADDMNKMFCDKSISGIFAI
RGGYGATRILDKLNYNMIKKNPKVFSGYSDVTAIHNVINARCDFITFHAPMPATEMYKGLDNYTEYYFKKSIFDSEPLGK
LKNTEDIKMRTLFKGKAEGRLVGGNLSVICSTLGTEYEIDTKGKILFLEEIDEYPYKIDRMIMQLKQSNKFKDASGIILG
KWTDCLPPKGRESLSLIQIFEELIAPERKPTLYDVCCGHCLPTLTLPLGAKIKINANSKEINILE
>Mature_305_residues
MRRPKPLKKGDKIAIVGLSSPTTEERLELSIKAMHDFGFEVVVGESCKSHYGYLAGSDEIRADDMNKMFCDKSISGIFAI
RGGYGATRILDKLNYNMIKKNPKVFSGYSDVTAIHNVINARCDFITFHAPMPATEMYKGLDNYTEYYFKKSIFDSEPLGK
LKNTEDIKMRTLFKGKAEGRLVGGNLSVICSTLGTEYEIDTKGKILFLEEIDEYPYKIDRMIMQLKQSNKFKDASGIILG
KWTDCLPPKGRESLSLIQIFEELIAPERKPTLYDVCCGHCLPTLTLPLGAKIKINANSKEINILE

Specific function: May be involved in the degradation of peptidoglycan by catalyzing the cleavage of the terminal D-alanine residue from cytoplasmic murein peptides [H]

COG id: COG1619

COG function: function code V; Uncharacterized proteins, homologs of microcin C7 resistance protein MccF

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S66 family [H]

Homologues:

Organism=Escherichia coli, GI1787441, Length=285, Percent_Identity=29.4736842105263, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003507 [H]

Pfam domain/function: PF02016 Peptidase_S66 [H]

EC number: NA

Molecular weight: Translated: 34326; Mature: 34326

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRPKPLKKGDKIAIVGLSSPTTEERLELSIKAMHDFGFEVVVGESCKSHYGYLAGSDEI
CCCCCCCCCCCEEEEEECCCCCCHHHHHHHEEEHHHCCEEEEECCCHHHHCCEECCCCCC
RADDMNKMFCDKSISGIFAIRGGYGATRILDKLNYNMIKKNPKVFSGYSDVTAIHNVINA
CCCCHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCHHHHCCCCEECCCHHHHHHHHHHHC
RCDFITFHAPMPATEMYKGLDNYTEYYFKKSIFDSEPLGKLKNTEDIKMRTLFKGKAEGR
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCE
LVGGNLSVICSTLGTEYEIDTKGKILFLEEIDEYPYKIDRMIMQLKQSNKFKDASGIILG
EECCCHHHHHHHCCCCEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEE
KWTDCLPPKGRESLSLIQIFEELIAPERKPTLYDVCCGHCLPTLTLPLGAKIKINANSKE
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCE
INILE
EEEEC
>Mature Secondary Structure
MRRPKPLKKGDKIAIVGLSSPTTEERLELSIKAMHDFGFEVVVGESCKSHYGYLAGSDEI
CCCCCCCCCCCEEEEEECCCCCCHHHHHHHEEEHHHCCEEEEECCCHHHHCCEECCCCCC
RADDMNKMFCDKSISGIFAIRGGYGATRILDKLNYNMIKKNPKVFSGYSDVTAIHNVINA
CCCCHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCHHHHCCCCEECCCHHHHHHHHHHHC
RCDFITFHAPMPATEMYKGLDNYTEYYFKKSIFDSEPLGKLKNTEDIKMRTLFKGKAEGR
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCE
LVGGNLSVICSTLGTEYEIDTKGKILFLEEIDEYPYKIDRMIMQLKQSNKFKDASGIILG
EECCCHHHHHHHCCCCEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEE
KWTDCLPPKGRESLSLIQIFEELIAPERKPTLYDVCCGHCLPTLTLPLGAKIKINANSKE
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCE
INILE
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]