| Definition | Clostridium botulinum B str. Eklund 17B, complete genome. |
|---|---|
| Accession | NC_010674 |
| Length | 3,800,327 |
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The map label for this gene is pulA [H]
Identifier: 187933645
GI number: 187933645
Start: 3064603
End: 3066561
Strand: Reverse
Name: pulA [H]
Synonym: CLL_A2978
Alternate gene names: 187933645
Gene position: 3066561-3064603 (Counterclockwise)
Preceding gene: 187933031
Following gene: 187934096
Centisome position: 80.69
GC content: 27.77
Gene sequence:
>1959_bases ATGATGCAATCTATATTATATGAACATGAAAATTATTGTGGCAAATTAGGGGCAATTTATTCTGAAGAAAAAACAAAGTT TATATTATGGGCTCCAATTTCAAAAAGTGTAAGAGTAATATTATATTACGAAGAAAAAAATGAAGTTTTTGATATGAAAA AAGGAGAAGAAGGGATATGGAGTTTAGAACTTTTGGGAGATTTTAATGGAGTTTATTATAATTATTTGGTTAATAATGAT CAAAATGAAATAGAAGTTGTAGATCCATATGCTAAAGCAGTAAATGTAAATGGAAATAGGGGAATGGTAATTGACTTAGA GTCTACTAATCCTAAAGATTGGGATGAAGATATTAAGCCCCAATTTACAAACGCAACAGATGCTATTATATATGAAATAC ATGTAAGAGATTTTTCAATAAGTAAAACATCAGGTGTTAATGATAAATTTAGAGGAAAGTATAATGGGGTTTATGAGAAG GAAACTAAGATTCCAGGAACTAATTACCCTACATGTTTAGAGTATATAAAAGAGTTAGGAATAACACATGTTCATTTAAT GCCAGCATTTGATTATGCAACAGTAGATGAATCTAATGTTAGTTCTGAAAATTATAATTGGGGATATGATCCTAAAAATT TCAATGTACCAGAAGGATCATATTCTTTAGACCCTTTTAATGGGGAAAAACGAATAATAGAATTTAAGAATATGATTAAA ACTTTACATGAACATGGTATAAGAGTGATTATGGATGTTGTATATAACCACACACATTCAACATATAGTTCTAATTTAAA TTGCATAGTCCCCAGTTATTACTATAGACAAAATAATGATGGGTGGTTTTCTAATGGGTCTGGATGCGGAAATGAATTAG CAACAGAAAGAAAGATGGTTAAAAAATTTATAATTGATTCATTATTATATTGGGCAAGTGAGTATCATATAGATGGTTTT AGATTTGACTTAATGGGATTATATGATATAGAAATTATGAAAGATATAAGAAAAAGCTTGGATAAAATAGATAAATCCAT ACTTATGTATGGTGAAGGTTGGACAGGTGGTGCATCTCCTTTACCAGAATGGGATAAATGTATTAAGTTTAATATTAGTA AATTTGGAGACATGAATATAGCAGCATTTAGTGATGATATTAGAGATGGAATAAAAGGAAATGTTTTTGATTCTCATTCA AAAGGTTTTATAAGTGGTGCAGGCATTTTAGAAGAGACTATTAAATTTGGAGTAGTAGCGTCTACAAAGCATAATCAAAT AAATTATGAAAGTTTAAAATATAGTAAATTTCCATGGGCAAATGAACCGTATCAAACAGTAACATATGCGTCTGCACATG ATAATTATACATTATGGGATAAGATATGTCTGTCTAATAATAATTTTTCAAAAGAAGATTTAAAAGCCATGAATAAATTG TCTGCGGCTATAATTTTAACTTCTCAAGGTATTCCATTTTTTCAAGCAGGAGAAGAATTTTTAAGAACTAAGAAAAATAA TGATGGAAGCTTAAATGGCAATAGTTATAATTCACCTGATAGTGTAAATGAATTACAATGGAATAGAACTGTAGAATATA AAGATGTTGTAGATTATTATAAAGGATTAATTAGGCTTAGAAAATTTTGCAAAGGGTTTAGAATGGAAAGTAGCAATGAG ATAAGAGAAAATTTAAGGTTCTTAGAACGGGAAAGGAATTTTTATAATGATAAAGTAGTTGGATTTAATATAGACTTAAA TTATTTACAGATTAATTGGAAACAAATTTGTGTAATATATAATGCGAATTTGAATTCAGTTGAAGTTAAACTAGACAAGA ATGGATGGAATATTATTGTTAATGAAAATGAATCTGGAATAAATAAATTATCATATGTAGATAATAATATAGTAAGAGTT CCTAAATTAAGTTGCTATGTATTAGTAAAAGAAGAATAA
Upstream 100 bases:
>100_bases GATAAACATTTAAACTCCTATAAAGAAAAGAGTTTAAATTGAGTGTGCATTAATAATTAGAAATAATTAATATATAAAAA GTATTAGAGGTGCTAAAAGT
Downstream 100 bases:
>100_bases GTATATTTTAGTGGAGTACTGATATATGTATAAACAAATCGTAGCAGAATTTTAGAAATTAAATTACAAAAATACTTCAT ATATAAACACATATTCAAAA
Product: pullulanase, type I
Products: NA
Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]
Number of amino acids: Translated: 652; Mature: 652
Protein sequence:
>652_residues MMQSILYEHENYCGKLGAIYSEEKTKFILWAPISKSVRVILYYEEKNEVFDMKKGEEGIWSLELLGDFNGVYYNYLVNND QNEIEVVDPYAKAVNVNGNRGMVIDLESTNPKDWDEDIKPQFTNATDAIIYEIHVRDFSISKTSGVNDKFRGKYNGVYEK ETKIPGTNYPTCLEYIKELGITHVHLMPAFDYATVDESNVSSENYNWGYDPKNFNVPEGSYSLDPFNGEKRIIEFKNMIK TLHEHGIRVIMDVVYNHTHSTYSSNLNCIVPSYYYRQNNDGWFSNGSGCGNELATERKMVKKFIIDSLLYWASEYHIDGF RFDLMGLYDIEIMKDIRKSLDKIDKSILMYGEGWTGGASPLPEWDKCIKFNISKFGDMNIAAFSDDIRDGIKGNVFDSHS KGFISGAGILEETIKFGVVASTKHNQINYESLKYSKFPWANEPYQTVTYASAHDNYTLWDKICLSNNNFSKEDLKAMNKL SAAIILTSQGIPFFQAGEEFLRTKKNNDGSLNGNSYNSPDSVNELQWNRTVEYKDVVDYYKGLIRLRKFCKGFRMESSNE IRENLRFLERERNFYNDKVVGFNIDLNYLQINWKQICVIYNANLNSVEVKLDKNGWNIIVNENESGINKLSYVDNNIVRV PKLSCYVLVKEE
Sequences:
>Translated_652_residues MMQSILYEHENYCGKLGAIYSEEKTKFILWAPISKSVRVILYYEEKNEVFDMKKGEEGIWSLELLGDFNGVYYNYLVNND QNEIEVVDPYAKAVNVNGNRGMVIDLESTNPKDWDEDIKPQFTNATDAIIYEIHVRDFSISKTSGVNDKFRGKYNGVYEK ETKIPGTNYPTCLEYIKELGITHVHLMPAFDYATVDESNVSSENYNWGYDPKNFNVPEGSYSLDPFNGEKRIIEFKNMIK TLHEHGIRVIMDVVYNHTHSTYSSNLNCIVPSYYYRQNNDGWFSNGSGCGNELATERKMVKKFIIDSLLYWASEYHIDGF RFDLMGLYDIEIMKDIRKSLDKIDKSILMYGEGWTGGASPLPEWDKCIKFNISKFGDMNIAAFSDDIRDGIKGNVFDSHS KGFISGAGILEETIKFGVVASTKHNQINYESLKYSKFPWANEPYQTVTYASAHDNYTLWDKICLSNNNFSKEDLKAMNKL SAAIILTSQGIPFFQAGEEFLRTKKNNDGSLNGNSYNSPDSVNELQWNRTVEYKDVVDYYKGLIRLRKFCKGFRMESSNE IRENLRFLERERNFYNDKVVGFNIDLNYLQINWKQICVIYNANLNSVEVKLDKNGWNIIVNENESGINKLSYVDNNIVRV PKLSCYVLVKEE >Mature_652_residues MMQSILYEHENYCGKLGAIYSEEKTKFILWAPISKSVRVILYYEEKNEVFDMKKGEEGIWSLELLGDFNGVYYNYLVNND QNEIEVVDPYAKAVNVNGNRGMVIDLESTNPKDWDEDIKPQFTNATDAIIYEIHVRDFSISKTSGVNDKFRGKYNGVYEK ETKIPGTNYPTCLEYIKELGITHVHLMPAFDYATVDESNVSSENYNWGYDPKNFNVPEGSYSLDPFNGEKRIIEFKNMIK TLHEHGIRVIMDVVYNHTHSTYSSNLNCIVPSYYYRQNNDGWFSNGSGCGNELATERKMVKKFIIDSLLYWASEYHIDGF RFDLMGLYDIEIMKDIRKSLDKIDKSILMYGEGWTGGASPLPEWDKCIKFNISKFGDMNIAAFSDDIRDGIKGNVFDSHS KGFISGAGILEETIKFGVVASTKHNQINYESLKYSKFPWANEPYQTVTYASAHDNYTLWDKICLSNNNFSKEDLKAMNKL SAAIILTSQGIPFFQAGEEFLRTKKNNDGSLNGNSYNSPDSVNELQWNRTVEYKDVVDYYKGLIRLRKFCKGFRMESSNE IRENLRFLERERNFYNDKVVGFNIDLNYLQINWKQICVIYNANLNSVEVKLDKNGWNIIVNENESGINKLSYVDNNIVRV PKLSCYVLVKEE
Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]
COG id: COG1523
COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glycosyl hydrolase 13 family [H]
Homologues:
Organism=Escherichia coli, GI2367229, Length=674, Percent_Identity=23.4421364985163, Blast_Score=131, Evalue=1e-31,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006047 - InterPro: IPR004193 - InterPro: IPR017853 - InterPro: IPR013781 - InterPro: IPR013783 - InterPro: IPR014756 - InterPro: IPR005323 - InterPro: IPR011840 [H]
Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48; PF03714 PUD [H]
EC number: =3.2.1.41 [H]
Molecular weight: Translated: 75297; Mature: 75297
Theoretical pI: Translated: 5.21; Mature: 5.21
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.4 %Cys (Translated Protein) 2.1 %Met (Translated Protein) 3.5 %Cys+Met (Translated Protein) 1.4 %Cys (Mature Protein) 2.1 %Met (Mature Protein) 3.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MMQSILYEHENYCGKLGAIYSEEKTKFILWAPISKSVRVILYYEEKNEVFDMKKGEEGIW CCHHHHHHHHHHHHHHCCEECCCCCEEEEEECCCCCEEEEEEECCCCCEECCCCCCCCEE SLELLGDFNGVYYNYLVNNDQNEIEVVDPYAKAVNVNGNRGMVIDLESTNPKDWDEDIKP EEEEEECCCCEEEEEEEECCCCCEEEECCCCEEEECCCCCEEEEEECCCCCCCCCCCCCC QFTNATDAIIYEIHVRDFSISKTSGVNDKFRGKYNGVYEKETKIPGTNYPTCLEYIKELG CCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCC ITHVHLMPAFDYATVDESNVSSENYNWGYDPKNFNVPEGSYSLDPFNGEKRIIEFKNMIK CEEEEEEECCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHH TLHEHGIRVIMDVVYNHTHSTYSSNLNCIVPSYYYRQNNDGWFSNGSGCGNELATERKMV HHHHCCHHEEHHHHHCCCCCHHCCCCCEEECCEEEEECCCCCCCCCCCCCHHHHHHHHHH KKFIIDSLLYWASEYHIDGFRFDLMGLYDIEIMKDIRKSLDKIDKSILMYGEGWTGGASP HHHHHHHHHHHHHHCCCCCEEEEEEEEEHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCC LPEWDKCIKFNISKFGDMNIAAFSDDIRDGIKGNVFDSHSKGFISGAGILEETIKFGVVA CCCHHHHHEECCCCCCCCEEEEECHHHHCCCCCCEECCCCCCCEECCHHHHHHHHHCEEE STKHNQINYESLKYSKFPWANEPYQTVTYASAHDNYTLWDKICLSNNNFSKEDLKAMNKL ECCCCCCCCCCEEECCCCCCCCCCCEEEEEECCCCCEEEHEEEECCCCCCHHHHHHHHHC SAAIILTSQGIPFFQAGEEFLRTKKNNDGSLNGNSYNSPDSVNELQWNRTVEYKDVVDYY EEEEEEEECCCCHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH KGLIRLRKFCKGFRMESSNEIRENLRFLERERNFYNDKVVGFNIDLNYLQINWKQICVIY HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEEEEEEEEEEE NANLNSVEVKLDKNGWNIIVNENESGINKLSYVDNNIVRVPKLSCYVLVKEE ECCCCEEEEEEECCCCEEEEECCCCCCCHHHHCCCCEEECCCEEEEEEEECC >Mature Secondary Structure MMQSILYEHENYCGKLGAIYSEEKTKFILWAPISKSVRVILYYEEKNEVFDMKKGEEGIW CCHHHHHHHHHHHHHHCCEECCCCCEEEEEECCCCCEEEEEEECCCCCEECCCCCCCCEE SLELLGDFNGVYYNYLVNNDQNEIEVVDPYAKAVNVNGNRGMVIDLESTNPKDWDEDIKP EEEEEECCCCEEEEEEEECCCCCEEEECCCCEEEECCCCCEEEEEECCCCCCCCCCCCCC QFTNATDAIIYEIHVRDFSISKTSGVNDKFRGKYNGVYEKETKIPGTNYPTCLEYIKELG CCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCC ITHVHLMPAFDYATVDESNVSSENYNWGYDPKNFNVPEGSYSLDPFNGEKRIIEFKNMIK CEEEEEEECCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHH TLHEHGIRVIMDVVYNHTHSTYSSNLNCIVPSYYYRQNNDGWFSNGSGCGNELATERKMV HHHHCCHHEEHHHHHCCCCCHHCCCCCEEECCEEEEECCCCCCCCCCCCCHHHHHHHHHH KKFIIDSLLYWASEYHIDGFRFDLMGLYDIEIMKDIRKSLDKIDKSILMYGEGWTGGASP HHHHHHHHHHHHHHCCCCCEEEEEEEEEHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCC LPEWDKCIKFNISKFGDMNIAAFSDDIRDGIKGNVFDSHSKGFISGAGILEETIKFGVVA CCCHHHHHEECCCCCCCCEEEEECHHHHCCCCCCEECCCCCCCEECCHHHHHHHHHCEEE STKHNQINYESLKYSKFPWANEPYQTVTYASAHDNYTLWDKICLSNNNFSKEDLKAMNKL ECCCCCCCCCCEEECCCCCCCCCCCEEEEEECCCCCEEEHEEEECCCCCCHHHHHHHHHC SAAIILTSQGIPFFQAGEEFLRTKKNNDGSLNGNSYNSPDSVNELQWNRTVEYKDVVDYY EEEEEEEECCCCHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH KGLIRLRKFCKGFRMESSNEIRENLRFLERERNFYNDKVVGFNIDLNYLQINWKQICVIY HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEEEEEEEEEEE NANLNSVEVKLDKNGWNIIVNENESGINKLSYVDNNIVRVPKLSCYVLVKEE ECCCCEEEEEEECCCCEEEEECCCCCCCHHHHCCCCEEECCCEEEEEEEECC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9453151; 10360571 [H]