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The map label for this gene is nirB [C]

Identifier: 187933514

GI number: 187933514

Start: 2991222

End: 2992079

Strand: Reverse

Name: nirB [C]

Synonym: CLL_A2910

Alternate gene names: 187933514

Gene position: 2992079-2991222 (Counterclockwise)

Preceding gene: 187934973

Following gene: 187935430

Centisome position: 78.73

GC content: 31.35

Gene sequence:

>858_bases
ATGGCAGTTAATGCAAAAAGACAAAAGGAATTAAAAGCTTTAGGATTTTTATTACAAAATGATAAGGAACATTTTGCAGT
AAGGTTTTTAGGTAGAGCAGGTAATTTTACAGTAGAGGAACTAAACAATATAAATGTTATTTCTAAAAAGTATGGTAGAG
ATTATTGCGGAATGACAACAAGACTTCAAATTGAAGTACCTTGGATAAAAGATGAAGATGTAGAAATGGTTATTGAAGAA
GCAAAGAAATTAGGATTAAGACATGGTGGTACAGGACAAAAATTTAGACCTTTAGTATCTTGTAAAGGTACTGTATGTCT
TCATGGAAACATTAATACACAAGAAATATGTAGACAATTAGAAGATAAATACTTTGGAACAGATACTCCTCATAAATGTA
AGGTAGCAGTAGTTGGTTGCGCTAACAATTGTGCAAAAGCTAATATAAATGATATTGGTATTATGGGAAGAACAGTACCA
CAGTTTGTTTTAGATAAGTGTGTTGGATGTGGATTATGTGTTAAGGCTTGTAGACAAAAAGCTTTAGAAGTAGTTGATAA
AAAAATAGTATATAATAAAGATTTATGTGTTGATTGTGGCGGATGTGTAAGAGCTTGTAAATTAGGTGCAGCTGTTGCAA
AAGAAAAAGGTGGAGAAATCTTTGTTGGGGGAAGATTTGGTAGAGGAATGAGAATCGGAGATTCTTTAGGAAAGATATTT
AAAGAAGAAGAAATAATTCCAATGGTTGATAAGATTGTGGATTATTACAGAGAAGTAGGACAACCTGGTGAAAGAATTTC
TAAGGTTATGGATAGAATGGGAAAAGAAGAATTTATAAATAATGTTTTAAAGAGATAG

Upstream 100 bases:

>100_bases
AATATTAAAAGATAAAAATAAAATCTAATAATATTAAAAACTATATTTATATAAAATTAACATAAATAAATATATATTTA
TTTCTAGGGAGGGCACACAC

Downstream 100 bases:

>100_bases
AATGTTATAAAAATACATTTTAAATAGTATTAAAAAAGTTCAAGTAGTAATTATTTTATACAACTTGAACTTTTTAATAT
ATAGAAAATTATAAGAATTT

Product: anaerobic sulfite reductase subunit C

Products: 3 NAD(P)+; NH4OH; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MAVNAKRQKELKALGFLLQNDKEHFAVRFLGRAGNFTVEELNNINVISKKYGRDYCGMTTRLQIEVPWIKDEDVEMVIEE
AKKLGLRHGGTGQKFRPLVSCKGTVCLHGNINTQEICRQLEDKYFGTDTPHKCKVAVVGCANNCAKANINDIGIMGRTVP
QFVLDKCVGCGLCVKACRQKALEVVDKKIVYNKDLCVDCGGCVRACKLGAAVAKEKGGEIFVGGRFGRGMRIGDSLGKIF
KEEEIIPMVDKIVDYYREVGQPGERISKVMDRMGKEEFINNVLKR

Sequences:

>Translated_285_residues
MAVNAKRQKELKALGFLLQNDKEHFAVRFLGRAGNFTVEELNNINVISKKYGRDYCGMTTRLQIEVPWIKDEDVEMVIEE
AKKLGLRHGGTGQKFRPLVSCKGTVCLHGNINTQEICRQLEDKYFGTDTPHKCKVAVVGCANNCAKANINDIGIMGRTVP
QFVLDKCVGCGLCVKACRQKALEVVDKKIVYNKDLCVDCGGCVRACKLGAAVAKEKGGEIFVGGRFGRGMRIGDSLGKIF
KEEEIIPMVDKIVDYYREVGQPGERISKVMDRMGKEEFINNVLKR
>Mature_284_residues
AVNAKRQKELKALGFLLQNDKEHFAVRFLGRAGNFTVEELNNINVISKKYGRDYCGMTTRLQIEVPWIKDEDVEMVIEEA
KKLGLRHGGTGQKFRPLVSCKGTVCLHGNINTQEICRQLEDKYFGTDTPHKCKVAVVGCANNCAKANINDIGIMGRTVPQ
FVLDKCVGCGLCVKACRQKALEVVDKKIVYNKDLCVDCGGCVRACKLGAAVAKEKGGEIFVGGRFGRGMRIGDSLGKIFK
EEEIIPMVDKIVDYYREVGQPGERISKVMDRMGKEEFINNVLKR

Specific function: Nitrate assimilation (denitrification); second step. [C]

COG id: COG2221

COG function: function code C; Dissimilatory sulfite reductase (desulfoviridin), alpha and beta subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR007516
- InterPro:   IPR007525
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF00037 Fer4; PF04432 FrhB_FdhB_C; PF04422 FrhB_FdhB_N; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: 1.7.1.4 [C]

Molecular weight: Translated: 31730; Mature: 31598

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
5.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVNAKRQKELKALGFLLQNDKEHFAVRFLGRAGNFTVEELNNINVISKKYGRDYCGMTT
CCCCCHHHHHHHHHHHHHHCCCHHEEHHEECCCCCEEHHHCCCCCHHHHHHCCHHCCCCE
RLQIEVPWIKDEDVEMVIEEAKKLGLRHGGTGQKFRPLVSCKGTVCLHGNINTQEICRQL
EEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCEEEEECCCCHHHHHHHH
EDKYFGTDTPHKCKVAVVGCANNCAKANINDIGIMGRTVPQFVLDKCVGCGLCVKACRQK
HHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHH
ALEVVDKKIVYNKDLCVDCGGCVRACKLGAAVAKEKGGEIFVGGRFGRGMRIGDSLGKIF
HHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHH
KEEEIIPMVDKIVDYYREVGQPGERISKVMDRMGKEEFINNVLKR
HCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
AVNAKRQKELKALGFLLQNDKEHFAVRFLGRAGNFTVEELNNINVISKKYGRDYCGMTT
CCCCHHHHHHHHHHHHHHCCCHHEEHHEECCCCCEEHHHCCCCCHHHHHHCCHHCCCCE
RLQIEVPWIKDEDVEMVIEEAKKLGLRHGGTGQKFRPLVSCKGTVCLHGNINTQEICRQL
EEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCEEEEECCCCHHHHHHHH
EDKYFGTDTPHKCKVAVVGCANNCAKANINDIGIMGRTVPQFVLDKCVGCGLCVKACRQK
HHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHH
ALEVVDKKIVYNKDLCVDCGGCVRACKLGAAVAKEKGGEIFVGGRFGRGMRIGDSLGKIF
HHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHH
KEEEIIPMVDKIVDYYREVGQPGERISKVMDRMGKEEFINNVLKR
HCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD; NAD+; NADH; Siroheme [C]

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.4 {NO2-}} 0.005 {NO2-}} 0.016 {NADH}} 5.3 {hydroxylamine}} [C]

Substrates: 3 NAD(P)H; nitrite [C]

Specific reaction: 3 NAD(P)H + nitrite = 3 NAD(P)+ + NH4OH + H2O [C]

General reaction: Redox reaction [C]

Inhibitor: 1, 10-Phenanthroline; 2, 2'-Bipyridine; Arsenite; CN-; NAD+; NADP+; NaN3; p-Chloromercuribenzoate; SO32- [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]