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The map label for this gene is oppA [H]

Identifier: 187933355

GI number: 187933355

Start: 2579730

End: 2581379

Strand: Reverse

Name: oppA [H]

Synonym: CLL_A2488

Alternate gene names: 187933355

Gene position: 2581379-2579730 (Counterclockwise)

Preceding gene: 187933451

Following gene: 187935684

Centisome position: 67.93

GC content: 31.82

Gene sequence:

>1650_bases
ATGAAAACTAGTAAAATTAAAAAGTTATGTGCAATAACATTAGCATTAACTTTAGGTTTTTCAAGCTTAGTAGGGTGTGG
AGGAGGATCTAGTAATGATCCAAATGCTCAAGTTATAACTTATAATTTAAATTCAGATCCTAAGACATTAGATCCAGCAT
TAAATGAAGCCGTAGAGAGTGGAACTGTTATAGTAAATGCATTTGAAGGATTGTATAAATTAGATGAGAATAATAAGGCT
ATTCCTGGAATTGCTGAAAAATGTGATATTTCAGAAGATGGAACAGTTTATACTTTCCATATAAGAGATGGAGTTAAGTG
GTCAAATGGAGATGTACTTACAGCAAATGATTTTGAATACTCATGGAAAAGAGTTTTAAATCCAGCAACAGCTTCAGGAT
ATGCATTCCAAATGTCATATATTAAAGGTGGAGAAGCTTATAATCAAGGAAAAGGAAGCCTAGAGGATGTAGGGATTAAA
GCAATTGATGATAAGACTTTAGAAGTAACATTAGCTGCTCCGTGTTCATACTTTTTAGAATTAACAGCATTTCCTTGTTA
CTTACCAGTAAATAAGGGGATAGTTGAAGCAAATAAAGATGCTTGGGCTCTTAATGCTGAAACCTACGTTTCAAATGGTC
CTTTTAAGATAACTAAATATGGAATTAAAGATGAAATTGTATTGGAAAAAAATGAAAATTACTATGATGCTAACAATATT
AAACTTGATAAATTAAATGTAAAATTAGTTGCTGAAGATACATCTGCTTGGGCTAGTTTCCAAACAGGAGATTTTGATAT
GGTAGATATTGTACCAACATCAGAAATAGAAGGAGCATTAGCTGATGGAACAGGAAAAAAATGTCCATTACTAGGAACAG
GATTTTATGTAATAAATGTTTCAGATTCAGTAAATGCAATTGATCCTAATGCTGCAAAAGCATTACAAGATAAGAGAGTT
AGAAAAGCTTTAAATCTTGCAATAGATAGACCTTCAATAATTAATAATATACTAAAAAATGGTTCAACACCAGCAACTGG
ATTTGTCCCACAAGGAATTAAAAATCCAAATGGAACAGATTTTGCTGATAAAAAATATTTTAAAGCAGAGGCAGATATAG
AAGAAGCTAAGAAATTATTAGCTGAAGCTGGATATCCAAATGGTGAAGGATTTCCTAATCTTGTAGTTCTTATTAACCCA
ACTAATCCGAATGGAGATGTTGCTCAAGCTATGCAAGATATGTGGAAAAATAATTTAGGAATTAATGTTGAACTTCAAAG
TCAAGAATGGAAAGTATTCCAAACTACAAGAACTGCAAAACAATATGAAGTTGCATTCCACGCTTGGGTTGGTGATTATA
TAGACCCAATGACATTCTTAGATATGTGGGAAAGTACATCAGGACAAAACTGTGCAGGATATAATAATCCAGAATATGAT
AAGTTAATAAGAGATGCAAAGGTTGAACAAGATCAAACTAAGAGATTTGAAATGCTGCATCAAGCAGAAGATATGTTAAT
GGAAGATATGTTTGTAATACCATTAAATTATCAAGTTAAGGTTAAGGGGATTAAAGACTACGTAAAAGGTGTTAATGTAT
CACCACTAGGATATATTTACTTTGATAAGGCATATGTAGAAGGAAAATAA

Upstream 100 bases:

>100_bases
TAAGGCTTTGCAATCGTTATCTTTCACTTGTTGTTGGAATAAAAAATGATTGTTAGTTATTAAGATAAATTTGTCAGAAT
TGTTAAGGGGGAAATTTTTA

Downstream 100 bases:

>100_bases
AATTTTAAGCAAAGTGCTGATTATGTATAAATAAAATGGACTGAGTAATTGAACTTAGGAATTCTCAAAGTCCAATTACA
ATGACATATTATCTATTACA

Product: oligopeptide-binding protein OppA

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: Stage 0 sporulation protein KA [H]

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MKTSKIKKLCAITLALTLGFSSLVGCGGGSSNDPNAQVITYNLNSDPKTLDPALNEAVESGTVIVNAFEGLYKLDENNKA
IPGIAEKCDISEDGTVYTFHIRDGVKWSNGDVLTANDFEYSWKRVLNPATASGYAFQMSYIKGGEAYNQGKGSLEDVGIK
AIDDKTLEVTLAAPCSYFLELTAFPCYLPVNKGIVEANKDAWALNAETYVSNGPFKITKYGIKDEIVLEKNENYYDANNI
KLDKLNVKLVAEDTSAWASFQTGDFDMVDIVPTSEIEGALADGTGKKCPLLGTGFYVINVSDSVNAIDPNAAKALQDKRV
RKALNLAIDRPSIINNILKNGSTPATGFVPQGIKNPNGTDFADKKYFKAEADIEEAKKLLAEAGYPNGEGFPNLVVLINP
TNPNGDVAQAMQDMWKNNLGINVELQSQEWKVFQTTRTAKQYEVAFHAWVGDYIDPMTFLDMWESTSGQNCAGYNNPEYD
KLIRDAKVEQDQTKRFEMLHQAEDMLMEDMFVIPLNYQVKVKGIKDYVKGVNVSPLGYIYFDKAYVEGK

Sequences:

>Translated_549_residues
MKTSKIKKLCAITLALTLGFSSLVGCGGGSSNDPNAQVITYNLNSDPKTLDPALNEAVESGTVIVNAFEGLYKLDENNKA
IPGIAEKCDISEDGTVYTFHIRDGVKWSNGDVLTANDFEYSWKRVLNPATASGYAFQMSYIKGGEAYNQGKGSLEDVGIK
AIDDKTLEVTLAAPCSYFLELTAFPCYLPVNKGIVEANKDAWALNAETYVSNGPFKITKYGIKDEIVLEKNENYYDANNI
KLDKLNVKLVAEDTSAWASFQTGDFDMVDIVPTSEIEGALADGTGKKCPLLGTGFYVINVSDSVNAIDPNAAKALQDKRV
RKALNLAIDRPSIINNILKNGSTPATGFVPQGIKNPNGTDFADKKYFKAEADIEEAKKLLAEAGYPNGEGFPNLVVLINP
TNPNGDVAQAMQDMWKNNLGINVELQSQEWKVFQTTRTAKQYEVAFHAWVGDYIDPMTFLDMWESTSGQNCAGYNNPEYD
KLIRDAKVEQDQTKRFEMLHQAEDMLMEDMFVIPLNYQVKVKGIKDYVKGVNVSPLGYIYFDKAYVEGK
>Mature_549_residues
MKTSKIKKLCAITLALTLGFSSLVGCGGGSSNDPNAQVITYNLNSDPKTLDPALNEAVESGTVIVNAFEGLYKLDENNKA
IPGIAEKCDISEDGTVYTFHIRDGVKWSNGDVLTANDFEYSWKRVLNPATASGYAFQMSYIKGGEAYNQGKGSLEDVGIK
AIDDKTLEVTLAAPCSYFLELTAFPCYLPVNKGIVEANKDAWALNAETYVSNGPFKITKYGIKDEIVLEKNENYYDANNI
KLDKLNVKLVAEDTSAWASFQTGDFDMVDIVPTSEIEGALADGTGKKCPLLGTGFYVINVSDSVNAIDPNAAKALQDKRV
RKALNLAIDRPSIINNILKNGSTPATGFVPQGIKNPNGTDFADKKYFKAEADIEEAKKLLAEAGYPNGEGFPNLVVLINP
TNPNGDVAQAMQDMWKNNLGINVELQSQEWKVFQTTRTAKQYEVAFHAWVGDYIDPMTFLDMWESTSGQNCAGYNNPEYD
KLIRDAKVEQDQTKRFEMLHQAEDMLMEDMFVIPLNYQVKVKGIKDYVKGVNVSPLGYIYFDKAYVEGK

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, It binds peptides up to five amino acids long with high affinity. Also required for sporulation and competence [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=518, Percent_Identity=33.011583011583, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1789397, Length=527, Percent_Identity=31.4990512333966, Blast_Score=261, Evalue=1e-70,
Organism=Escherichia coli, GI87081878, Length=538, Percent_Identity=31.2267657992565, Blast_Score=258, Evalue=8e-70,
Organism=Escherichia coli, GI1789966, Length=562, Percent_Identity=26.1565836298932, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1787762, Length=512, Percent_Identity=24.8046875, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1787052, Length=537, Percent_Identity=23.6499068901304, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787551, Length=506, Percent_Identity=21.3438735177866, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60467; Mature: 60467

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSKIKKLCAITLALTLGFSSLVGCGGGSSNDPNAQVITYNLNSDPKTLDPALNEAVES
CCCHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHC
GTVIVNAFEGLYKLDENNKAIPGIAEKCDISEDGTVYTFHIRDGVKWSNGDVLTANDFEY
CEEEEEECCCCEEECCCCCCCCCCHHHCCCCCCCCEEEEEECCCCEECCCCEEEECCCCH
SWKRVLNPATASGYAFQMSYIKGGEAYNQGKGSLEDVGIKAIDDKTLEVTLAAPCSYFLE
HHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHCCEEEECCCEEEEEEECCHHHHHH
LTAFPCYLPVNKGIVEANKDAWALNAETYVSNGPFKITKYGIKDEIVLEKNENYYDANNI
HHCCEEEEECCCCEEECCCCEEEEECHHEECCCCEEEEEECCCCEEEEECCCCEECCCCE
KLDKLNVKLVAEDTSAWASFQTGDFDMVDIVPTSEIEGALADGTGKKCPLLGTGFYVINV
EEEEEEEEEEEECCCCCEEECCCCEEEEEEEECCCCCCEEECCCCCCCCEEECCEEEEEE
SDSVNAIDPNAAKALQDKRVRKALNLAIDRPSIINNILKNGSTPATGFVPQGIKNPNGTD
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
FADKKYFKAEADIEEAKKLLAEAGYPNGEGFPNLVVLINPTNPNGDVAQAMQDMWKNNLG
CCCCCCEECCCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCC
INVELQSQEWKVFQTTRTAKQYEVAFHAWVGDYIDPMTFLDMWESTSGQNCAGYNNPEYD
EEEEECCCCCEEEEEHHCCHHHEEEEEEHHHHHCCHHHHHHHHCCCCCCCCCCCCCCHHH
KLIRDAKVEQDQTKRFEMLHQAEDMLMEDMFVIPLNYQVKVKGIKDYVKGVNVSPLGYIY
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEEECCEEEEEEEHHHHHCCCCCCCEEEEE
FDKAYVEGK
EEEEEECCC
>Mature Secondary Structure
MKTSKIKKLCAITLALTLGFSSLVGCGGGSSNDPNAQVITYNLNSDPKTLDPALNEAVES
CCCHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHC
GTVIVNAFEGLYKLDENNKAIPGIAEKCDISEDGTVYTFHIRDGVKWSNGDVLTANDFEY
CEEEEEECCCCEEECCCCCCCCCCHHHCCCCCCCCEEEEEECCCCEECCCCEEEECCCCH
SWKRVLNPATASGYAFQMSYIKGGEAYNQGKGSLEDVGIKAIDDKTLEVTLAAPCSYFLE
HHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHCCEEEECCCEEEEEEECCHHHHHH
LTAFPCYLPVNKGIVEANKDAWALNAETYVSNGPFKITKYGIKDEIVLEKNENYYDANNI
HHCCEEEEECCCCEEECCCCEEEEECHHEECCCCEEEEEECCCCEEEEECCCCEECCCCE
KLDKLNVKLVAEDTSAWASFQTGDFDMVDIVPTSEIEGALADGTGKKCPLLGTGFYVINV
EEEEEEEEEEEECCCCCEEECCCCEEEEEEEECCCCCCEEECCCCCCCCEEECCEEEEEE
SDSVNAIDPNAAKALQDKRVRKALNLAIDRPSIINNILKNGSTPATGFVPQGIKNPNGTD
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
FADKKYFKAEADIEEAKKLLAEAGYPNGEGFPNLVVLINPTNPNGDVAQAMQDMWKNNLG
CCCCCCEECCCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCC
INVELQSQEWKVFQTTRTAKQYEVAFHAWVGDYIDPMTFLDMWESTSGQNCAGYNNPEYD
EEEEECCCCCEEEEEHHCCHHHEEEEEEHHHHHCCHHHHHHHHCCCCCCCCCCCCCCHHH
KLIRDAKVEQDQTKRFEMLHQAEDMLMEDMFVIPLNYQVKVKGIKDYVKGVNVSPLGYIY
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEEECCEEEEEEEHHHHHCCCCCCCEEEEE
FDKAYVEGK
EEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1901616; 1899858; 9384377 [H]