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The map label for this gene is eno [H]

Identifier: 187933291

GI number: 187933291

Start: 3156667

End: 3157962

Strand: Reverse

Name: eno [H]

Synonym: CLL_A3055

Alternate gene names: 187933291

Gene position: 3157962-3156667 (Counterclockwise)

Preceding gene: 187935323

Following gene: 187932521

Centisome position: 83.1

GC content: 31.71

Gene sequence:

>1296_bases
ATGAACGATTACTTAGAAATAATAGACGTCGTTGCAAGACAAATATTAGATTCAAGATGCTTTCCAACTGTAGAAGTAGA
AGTATATCTTGAAGATGGAACAATAGGAAGAGCTGCAGTACCATCAGGGGCATCAACTGGTATGTATGAAGCAGTAGAGT
TAAGAGATGGAGACAAAGATAAATATTTAGGTAAAGGTGTATTAACTGCAGTACAAAATGTTAATGATACAATTGCTGAA
GCTTTAATAGGTTGTAATGTATTTGATCAACCATATATAGATAAAATGCTTATAGAATTAGATGGAACTGATAATAAAGG
AAAACTAGGGGCTAATGCAATCTTAGGAGTGTCATTAGCTGTTGCAAATGCTGCTGCTAATGCACTAGGATTATCTTTAT
ATAAATATGTTGGCGGTGTAAATGCAAAAGTTTTACCAGTACCTATGATGAACATAATAAATGGTGGATCACATGCTGAT
AATTCAGTAGATCTACAAGAATTTATGATTATGCCAGTAGGTGCTACAAGTTTTACTGAAGCTTTAAGAATGTGTGCTGA
AGTATACCATACATTAAAAAATACTCTTAAAGATAAAGGATATGCTACTGGACTTGGAGATGAGGGTGGATTTGCTCCTA
ATTTAAAATCAAATGCAGAAGCTATTGATGTTATTATAGAAGCAATAACTAAAGCAGGATATAAAGCCGGAGAAGATATG
TTCATTGCTATAGATGCAGCATCTTCAGAATATTATAAAGATGGAAAATATGTATTAGAAAATGAAGGCAAAACATTGAC
ATCAGCTGAAATGGTTGATTTCTTTGAAGATTGGGTGAATAAATATCCAATAATTTCAATTGAAGATGGTATGGCAGAAG
AAGATTGGGATGGATGGAAGTTGTTAAATGAAAGATTAGGAAAGAAAGTTCAATTAGTAGGTGATGATTTATTTGTTACT
AATACTGAAAGATTAGAAAAAGGAATAGAAATTGGTGCAGCCAATTCAATTCTTATAAAATTAAACCAAATTGGTACATT
AACTGAAACTTTAAATGCAATAGAAATGGCTAATAGAGCAGGATATACTGCTGTTATTTCTCATAGATCTGGAGAAACTG
AAGATACTACAATTTCTGACTTAGTTGTTGCAGTTAATGCAGGACAAATAAAAACAGGTGCACCAGCTAGATCTGAAAGA
GTTGCAAAATACAATCAATTATTAAGAATAGAAGAAGAATTAGAAGATGTAGCTGAATATAGAGGAAAAAAAGCATTTTT
TAATATAAAAAAATAA

Upstream 100 bases:

>100_bases
AGGTAATTAAAAAAATCTTTTCATTTTTATGTTTTATATATATAAATGAAATTGATAAATATTATCAAATAATTTCATAT
AATTCAAGGAGGGTAATTAA

Downstream 100 bases:

>100_bases
ATTTATAAAATGAAATAAGATATATAGCATCTAACAAAACTAAAAAACGGCTGCAATTTGTAGCTGTTTTTTAGTTTTAT
AATTTCTAGAAGATGTTAAT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MNDYLEIIDVVARQILDSRCFPTVEVEVYLEDGTIGRAAVPSGASTGMYEAVELRDGDKDKYLGKGVLTAVQNVNDTIAE
ALIGCNVFDQPYIDKMLIELDGTDNKGKLGANAILGVSLAVANAAANALGLSLYKYVGGVNAKVLPVPMMNIINGGSHAD
NSVDLQEFMIMPVGATSFTEALRMCAEVYHTLKNTLKDKGYATGLGDEGGFAPNLKSNAEAIDVIIEAITKAGYKAGEDM
FIAIDAASSEYYKDGKYVLENEGKTLTSAEMVDFFEDWVNKYPIISIEDGMAEEDWDGWKLLNERLGKKVQLVGDDLFVT
NTERLEKGIEIGAANSILIKLNQIGTLTETLNAIEMANRAGYTAVISHRSGETEDTTISDLVVAVNAGQIKTGAPARSER
VAKYNQLLRIEEELEDVAEYRGKKAFFNIKK

Sequences:

>Translated_431_residues
MNDYLEIIDVVARQILDSRCFPTVEVEVYLEDGTIGRAAVPSGASTGMYEAVELRDGDKDKYLGKGVLTAVQNVNDTIAE
ALIGCNVFDQPYIDKMLIELDGTDNKGKLGANAILGVSLAVANAAANALGLSLYKYVGGVNAKVLPVPMMNIINGGSHAD
NSVDLQEFMIMPVGATSFTEALRMCAEVYHTLKNTLKDKGYATGLGDEGGFAPNLKSNAEAIDVIIEAITKAGYKAGEDM
FIAIDAASSEYYKDGKYVLENEGKTLTSAEMVDFFEDWVNKYPIISIEDGMAEEDWDGWKLLNERLGKKVQLVGDDLFVT
NTERLEKGIEIGAANSILIKLNQIGTLTETLNAIEMANRAGYTAVISHRSGETEDTTISDLVVAVNAGQIKTGAPARSER
VAKYNQLLRIEEELEDVAEYRGKKAFFNIKK
>Mature_431_residues
MNDYLEIIDVVARQILDSRCFPTVEVEVYLEDGTIGRAAVPSGASTGMYEAVELRDGDKDKYLGKGVLTAVQNVNDTIAE
ALIGCNVFDQPYIDKMLIELDGTDNKGKLGANAILGVSLAVANAAANALGLSLYKYVGGVNAKVLPVPMMNIINGGSHAD
NSVDLQEFMIMPVGATSFTEALRMCAEVYHTLKNTLKDKGYATGLGDEGGFAPNLKSNAEAIDVIIEAITKAGYKAGEDM
FIAIDAASSEYYKDGKYVLENEGKTLTSAEMVDFFEDWVNKYPIISIEDGMAEEDWDGWKLLNERLGKKVQLVGDDLFVT
NTERLEKGIEIGAANSILIKLNQIGTLTETLNAIEMANRAGYTAVISHRSGETEDTTISDLVVAVNAGQIKTGAPARSER
VAKYNQLLRIEEELEDVAEYRGKKAFFNIKK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=54.1860465116279, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI4503571, Length=431, Percent_Identity=50.8120649651972, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI301897477, Length=433, Percent_Identity=51.7321016166282, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI301897469, Length=433, Percent_Identity=51.7321016166282, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI301897479, Length=431, Percent_Identity=46.6357308584687, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=27.6785714285714, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=27.6785714285714, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=27.6785714285714, Blast_Score=108, Evalue=7e-24,
Organism=Escherichia coli, GI1789141, Length=424, Percent_Identity=62.2641509433962, Blast_Score=511, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI71995829, Length=432, Percent_Identity=53.4722222222222, Blast_Score=437, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17536383, Length=432, Percent_Identity=53.4722222222222, Blast_Score=435, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI32563855, Length=187, Percent_Identity=47.5935828877005, Blast_Score=186, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6321693, Length=434, Percent_Identity=52.073732718894, Blast_Score=416, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6324974, Length=435, Percent_Identity=50.8045977011494, Blast_Score=404, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6324969, Length=435, Percent_Identity=50.8045977011494, Blast_Score=404, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6323985, Length=435, Percent_Identity=50.5747126436782, Blast_Score=402, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6321968, Length=434, Percent_Identity=50.9216589861751, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580918, Length=432, Percent_Identity=52.3148148148148, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580916, Length=432, Percent_Identity=52.3148148148148, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580920, Length=432, Percent_Identity=52.3148148148148, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580914, Length=432, Percent_Identity=52.3148148148148, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI281360527, Length=432, Percent_Identity=52.3148148148148, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI17137654, Length=432, Percent_Identity=52.3148148148148, Blast_Score=410, Evalue=1e-115,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46790; Mature: 46790

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDYLEIIDVVARQILDSRCFPTVEVEVYLEDGTIGRAAVPSGASTGMYEAVELRDGDKD
CCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCH
KYLGKGVLTAVQNVNDTIAEALIGCNVFDQPYIDKMLIELDGTDNKGKLGANAILGVSLA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHH
VANAAANALGLSLYKYVGGVNAKVLPVPMMNIINGGSHADNSVDLQEFMIMPVGATSFTE
HHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHCCCCCCCCCCCHHHHEEECCCCHHHHH
ALRMCAEVYHTLKNTLKDKGYATGLGDEGGFAPNLKSNAEAIDVIIEAITKAGYKAGEDM
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCE
FIAIDAASSEYYKDGKYVLENEGKTLTSAEMVDFFEDWVNKYPIISIEDGMAEEDWDGWK
EEEEECCCCCHHCCCCEEEECCCCEEHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHH
LLNERLGKKVQLVGDDLFVTNTERLEKGIEIGAANSILIKLNQIGTLTETLNAIEMANRA
HHHHHCCCEEEEECCCEEEECHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCC
GYTAVISHRSGETEDTTISDLVVAVNAGQIKTGAPARSERVAKYNQLLRIEEELEDVAEY
CCEEEEECCCCCCCCCCHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RGKKAFFNIKK
CCCEEEEECCC
>Mature Secondary Structure
MNDYLEIIDVVARQILDSRCFPTVEVEVYLEDGTIGRAAVPSGASTGMYEAVELRDGDKD
CCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCH
KYLGKGVLTAVQNVNDTIAEALIGCNVFDQPYIDKMLIELDGTDNKGKLGANAILGVSLA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHH
VANAAANALGLSLYKYVGGVNAKVLPVPMMNIINGGSHADNSVDLQEFMIMPVGATSFTE
HHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHCCCCCCCCCCCHHHHEEECCCCHHHHH
ALRMCAEVYHTLKNTLKDKGYATGLGDEGGFAPNLKSNAEAIDVIIEAITKAGYKAGEDM
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCE
FIAIDAASSEYYKDGKYVLENEGKTLTSAEMVDFFEDWVNKYPIISIEDGMAEEDWDGWK
EEEEECCCCCHHCCCCEEEECCCCEEHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHH
LLNERLGKKVQLVGDDLFVTNTERLEKGIEIGAANSILIKLNQIGTLTETLNAIEMANRA
HHHHHCCCEEEEECCCEEEECHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCC
GYTAVISHRSGETEDTTISDLVVAVNAGQIKTGAPARSERVAKYNQLLRIEEELEDVAEY
CCEEEEECCCCCCCCCCHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RGKKAFFNIKK
CCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA