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The map label for this gene is potB [H]

Identifier: 187933229

GI number: 187933229

Start: 1004886

End: 1005728

Strand: Reverse

Name: potB [H]

Synonym: CLL_A0995

Alternate gene names: 187933229

Gene position: 1005728-1004886 (Counterclockwise)

Preceding gene: 187934240

Following gene: 187932927

Centisome position: 26.46

GC content: 27.28

Gene sequence:

>843_bases
ATGCGTAAAAAAAGTACTTCGATTTTAGCATATCCTTATTTTATATGGAGTGCGCTATTTATAGTTATCCCTTTAATTAT
AGTTTTATTTTTTAGCTTTACTACAAAAACTAGTTCAGGATATTCATTTTCTTTAGAAAATTATAATAACTTATTTAATT
TTCAATATTTATATATATTTAAACAATCTATATTATTAGCTTTAGAATCAACTGTAGCTTGTTTAGTTATTGGATATCCC
GCTTCTTATATAATTTCAAAAATGAACTTAAGTAAGAGCAATTTTTTAATAATGTTATTTATATTGCCAATGTGGATGAA
TTTTTTATTGAGAACCTATGCTTGGGTACCTATCTTAGGAAAAACAGGTTTAATAAATTTGTTACTTGGTAAAATAGGTA
TAGGACCTATTTCTTTTCTTTATAATGATGGTGCTGTTTTACTAGGAATGATTTATAACTTCTTACCATTTATGGTTCTT
CCTATTTATACAATATTAATAAAGATGGACCAAGATTTAATAAATGCTGCGCATGATTTAGGTGCTAACAGATTTAAAGT
ATTTACAAGAGTTGTTTTACCACTTAGTATGCCTGGAATAATTTCTGGAATCACAATGGTGTTTATGCCAGCAGTTTCTA
CCTTTGTAATCTCAAGATTATTAGGTGGAGGTCAATATATGTTAATTGGTAATGTAATTGAACAACAATTCACAACCATG
GGTGATTGGAATTTTGGATCTGCAATCTCAATATTAATGATGATAATTATATTAATATTTATGAGAATTGCTTCTAAATT
CGAAAATGATGACAGCAAAGAAGGTGGTGGACAATTATGGTAA

Upstream 100 bases:

>100_bases
ACATTCATTCTTCACAATACAAAATCTGTAGATGTTGATAATGAAATAGGATTAGATATCTATCCTGAAGATATACATAT
TATGAAAAAGAGTGATGATA

Downstream 100 bases:

>100_bases
GTAAACTAGAAAGTTTTATTAAAAAATTCTATTTAGTTATTATATTTTTATTCTTATATACGCCTATCATTACTTTGATG
GTATTTTCATTTAATAGTTC

Product: putrescine transport system permease protein PotH

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MRKKSTSILAYPYFIWSALFIVIPLIIVLFFSFTTKTSSGYSFSLENYNNLFNFQYLYIFKQSILLALESTVACLVIGYP
ASYIISKMNLSKSNFLIMLFILPMWMNFLLRTYAWVPILGKTGLINLLLGKIGIGPISFLYNDGAVLLGMIYNFLPFMVL
PIYTILIKMDQDLINAAHDLGANRFKVFTRVVLPLSMPGIISGITMVFMPAVSTFVISRLLGGGQYMLIGNVIEQQFTTM
GDWNFGSAISILMMIIILIFMRIASKFENDDSKEGGGQLW

Sequences:

>Translated_280_residues
MRKKSTSILAYPYFIWSALFIVIPLIIVLFFSFTTKTSSGYSFSLENYNNLFNFQYLYIFKQSILLALESTVACLVIGYP
ASYIISKMNLSKSNFLIMLFILPMWMNFLLRTYAWVPILGKTGLINLLLGKIGIGPISFLYNDGAVLLGMIYNFLPFMVL
PIYTILIKMDQDLINAAHDLGANRFKVFTRVVLPLSMPGIISGITMVFMPAVSTFVISRLLGGGQYMLIGNVIEQQFTTM
GDWNFGSAISILMMIIILIFMRIASKFENDDSKEGGGQLW
>Mature_280_residues
MRKKSTSILAYPYFIWSALFIVIPLIIVLFFSFTTKTSSGYSFSLENYNNLFNFQYLYIFKQSILLALESTVACLVIGYP
ASYIISKMNLSKSNFLIMLFILPMWMNFLLRTYAWVPILGKTGLINLLLGKIGIGPISFLYNDGAVLLGMIYNFLPFMVL
PIYTILIKMDQDLINAAHDLGANRFKVFTRVVLPLSMPGIISGITMVFMPAVSTFVISRLLGGGQYMLIGNVIEQQFTTM
GDWNFGSAISILMMIIILIFMRIASKFENDDSKEGGGQLW

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=267, Percent_Identity=37.4531835205993, Blast_Score=188, Evalue=4e-49,
Organism=Escherichia coli, GI1787080, Length=223, Percent_Identity=41.2556053811659, Blast_Score=175, Evalue=2e-45,
Organism=Escherichia coli, GI1787713, Length=258, Percent_Identity=27.1317829457364, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31599; Mature: 31599

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKKSTSILAYPYFIWSALFIVIPLIIVLFFSFTTKTSSGYSFSLENYNNLFNFQYLYIF
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHH
KQSILLALESTVACLVIGYPASYIISKMNLSKSNFLIMLFILPMWMNFLLRTYAWVPILG
HHHHHHHHHHHHEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
KTGLINLLLGKIGIGPISFLYNDGAVLLGMIYNFLPFMVLPIYTILIKMDQDLINAAHDL
CCHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GANRFKVFTRVVLPLSMPGIISGITMVFMPAVSTFVISRLLGGGQYMLIGNVIEQQFTTM
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHC
GDWNFGSAISILMMIIILIFMRIASKFENDDSKEGGGQLW
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRKKSTSILAYPYFIWSALFIVIPLIIVLFFSFTTKTSSGYSFSLENYNNLFNFQYLYIF
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHH
KQSILLALESTVACLVIGYPASYIISKMNLSKSNFLIMLFILPMWMNFLLRTYAWVPILG
HHHHHHHHHHHHEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
KTGLINLLLGKIGIGPISFLYNDGAVLLGMIYNFLPFMVLPIYTILIKMDQDLINAAHDL
CCHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GANRFKVFTRVVLPLSMPGIISGITMVFMPAVSTFVISRLLGGGQYMLIGNVIEQQFTTM
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHC
GDWNFGSAISILMMIIILIFMRIASKFENDDSKEGGGQLW
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]