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The map label for this gene is dnaB [H]

Identifier: 187932892

GI number: 187932892

Start: 3778157

End: 3779494

Strand: Reverse

Name: dnaB [H]

Synonym: CLL_A3580

Alternate gene names: 187932892

Gene position: 3779494-3778157 (Counterclockwise)

Preceding gene: 187933433

Following gene: 187934225

Centisome position: 99.45

GC content: 32.21

Gene sequence:

>1338_bases
TTGGAATCACAAGTTATGAGAACTTTACCTCAGAGCATAGAAGCTGAACAATCGGTAATAGGATCTATGATTATAGATAA
ATCTGCTATTGCTAAAGTTTTAGAAAGCTTGGAAGAAGAAGATTTCTATAGAGATGGCCATAAAGTAATATTTAGAGCTA
TATTAGATATGTTTAGAAAAGACATGGGTGTAGATTTAATTACTTTATTGGAAAATTTAAAAAGTACGGAAATGCTTGAA
AGAGCAGGTGGAGTAACTTATATAACAGAAATAAGTGCATCAGTTCCTACTACTGCTAATTTATCTTCTTATATAAAAAT
AGTAGAAGAAAAGTCAGTTTTAAGAAAACTTATAAAAGCGTCAACATCGATAATAGAATCCAGCTATAATAATCAAGGCG
AAGTTGAAAATGTATTAGACGGAGCTGAAAAAAGAATATTTGATATAGCTGAAAAGAAAACATCAAAGGATTTTGAACCG
CTAAGTGATGTCTTAGAAAGAGGTTTTCTTGAAATAGAAAGACTTTTTAATAATAGGGGTGAAATTACAGGTGTAGGATC
TGGTTTTTCAGATTTAGATTCTAAAACATCAGGATTTCAAAAAGGTGATATGATTCTTATTGCTGCTAGACCATCTATGG
GTAAGACTACATTTGCATTAAATATAGCAGAGCATGCGGCGTTAAGAGAAGGAAAAAGCGTAGTTGTATTTTCTTTAGAA
ATGTCAAAAGAACAATTAGCATACAAATTGCTTTGTTCTGAAGCAAATGTTGATATGTTAAGACTTAGAACAGGTGAGTT
AGATGATAAGGATTGGGAGAACATAGCTAGAGCGACAGGTCCATTATCAAAAGCAAAAGTTTATATAGATGATACTGCAG
GGGTTTCAGTTATGGAAATGAGATCTAAATGTAGAAGATTAAAAATGGAATATGGAATAGATTTAATAGTTATAGATTAC
TTGCAACTTATGTCTGGAAGTACTAGTAGTGATAATAGACAACAAGAAGTGTCAGAAATATCAAGATCGATAAAAGCACT
GGCAAAGGAAATGGAATGTCCAGTTATAGCATTATCACAATTATCGCGTGCTCCAGAACAAAGAGCAGATCATAGACCAA
TGTTATCTGATTTAAGAGAATCTGGTTCTATAGAACAAGATGCTGACATTGTTATGTTCTTATATAGAGATGAGTATTAT
GACAAAGAAACTGAAGATAAGAATATTGCTGAATGCATAATAGCTAAACAAAGAAATGGTCCAGTGGGAACAGCTAAACT
CGCGTGGCTTGGTCAATTCAGTAAATTTGGGAATTTAGATGTTATTCATAAAGAATAA

Upstream 100 bases:

>100_bases
TTGTTTTTGAATAATGAAATAGGCTTCCTTGAACTAGGAAGCTTATTTTAAATTAAAGTTTAGATTGTGTTTATTAAATT
AAAAAAAAGGAGGACTGTTT

Downstream 100 bases:

>100_bases
AAATATTTACATTACTATTTTTAATTTACTAGTTAAAAATAATAATATATAAGGTAATATATATTATTATTGAAAAATTG
AGAATGAGCGTATTTAGTTT

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MESQVMRTLPQSIEAEQSVIGSMIIDKSAIAKVLESLEEEDFYRDGHKVIFRAILDMFRKDMGVDLITLLENLKSTEMLE
RAGGVTYITEISASVPTTANLSSYIKIVEEKSVLRKLIKASTSIIESSYNNQGEVENVLDGAEKRIFDIAEKKTSKDFEP
LSDVLERGFLEIERLFNNRGEITGVGSGFSDLDSKTSGFQKGDMILIAARPSMGKTTFALNIAEHAALREGKSVVVFSLE
MSKEQLAYKLLCSEANVDMLRLRTGELDDKDWENIARATGPLSKAKVYIDDTAGVSVMEMRSKCRRLKMEYGIDLIVIDY
LQLMSGSTSSDNRQQEVSEISRSIKALAKEMECPVIALSQLSRAPEQRADHRPMLSDLRESGSIEQDADIVMFLYRDEYY
DKETEDKNIAECIIAKQRNGPVGTAKLAWLGQFSKFGNLDVIHKE

Sequences:

>Translated_445_residues
MESQVMRTLPQSIEAEQSVIGSMIIDKSAIAKVLESLEEEDFYRDGHKVIFRAILDMFRKDMGVDLITLLENLKSTEMLE
RAGGVTYITEISASVPTTANLSSYIKIVEEKSVLRKLIKASTSIIESSYNNQGEVENVLDGAEKRIFDIAEKKTSKDFEP
LSDVLERGFLEIERLFNNRGEITGVGSGFSDLDSKTSGFQKGDMILIAARPSMGKTTFALNIAEHAALREGKSVVVFSLE
MSKEQLAYKLLCSEANVDMLRLRTGELDDKDWENIARATGPLSKAKVYIDDTAGVSVMEMRSKCRRLKMEYGIDLIVIDY
LQLMSGSTSSDNRQQEVSEISRSIKALAKEMECPVIALSQLSRAPEQRADHRPMLSDLRESGSIEQDADIVMFLYRDEYY
DKETEDKNIAECIIAKQRNGPVGTAKLAWLGQFSKFGNLDVIHKE
>Mature_445_residues
MESQVMRTLPQSIEAEQSVIGSMIIDKSAIAKVLESLEEEDFYRDGHKVIFRAILDMFRKDMGVDLITLLENLKSTEMLE
RAGGVTYITEISASVPTTANLSSYIKIVEEKSVLRKLIKASTSIIESSYNNQGEVENVLDGAEKRIFDIAEKKTSKDFEP
LSDVLERGFLEIERLFNNRGEITGVGSGFSDLDSKTSGFQKGDMILIAARPSMGKTTFALNIAEHAALREGKSVVVFSLE
MSKEQLAYKLLCSEANVDMLRLRTGELDDKDWENIARATGPLSKAKVYIDDTAGVSVMEMRSKCRRLKMEYGIDLIVIDY
LQLMSGSTSSDNRQQEVSEISRSIKALAKEMECPVIALSQLSRAPEQRADHRPMLSDLRESGSIEQDADIVMFLYRDEYY
DKETEDKNIAECIIAKQRNGPVGTAKLAWLGQFSKFGNLDVIHKE

Specific function: Participates In Initiation And Elongation During Chromosome Replication; It Exhibits DNA-Dependent ATPase Activity And Contains Distinct Active Sites For ATP Binding, DNA Binding, And Interaction With DNAc Protein, Primase, And Other Prepriming Proteins.

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=439, Percent_Identity=48.74715261959, Blast_Score=422, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 49827; Mature: 49827

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESQVMRTLPQSIEAEQSVIGSMIIDKSAIAKVLESLEEEDFYRDGHKVIFRAILDMFRK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DMGVDLITLLENLKSTEMLERAGGVTYITEISASVPTTANLSSYIKIVEEKSVLRKLIKA
HCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
STSIIESSYNNQGEVENVLDGAEKRIFDIAEKKTSKDFEPLSDVLERGFLEIERLFNNRG
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCC
EITGVGSGFSDLDSKTSGFQKGDMILIAARPSMGKTTFALNIAEHAALREGKSVVVFSLE
CEEECCCCHHHHCHHHCCCCCCCEEEEEECCCCCCCEEEEEHHHHHHHHCCCEEEEEEEC
MSKEQLAYKLLCSEANVDMLRLRTGELDDKDWENIARATGPLSKAKVYIDDTAGVSVMEM
CCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHH
RSKCRRLKMEYGIDLIVIDYLQLMSGSTSSDNRQQEVSEISRSIKALAKEMECPVIALSQ
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LSRAPEQRADHRPMLSDLRESGSIEQDADIVMFLYRDEYYDKETEDKNIAECIIAKQRNG
HHCCHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEEEHHHCCCCCCCCHHHHHHHHHCCCC
PVGTAKLAWLGQFSKFGNLDVIHKE
CCCHHHHHHHHHHHHCCCEEEEECC
>Mature Secondary Structure
MESQVMRTLPQSIEAEQSVIGSMIIDKSAIAKVLESLEEEDFYRDGHKVIFRAILDMFRK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DMGVDLITLLENLKSTEMLERAGGVTYITEISASVPTTANLSSYIKIVEEKSVLRKLIKA
HCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
STSIIESSYNNQGEVENVLDGAEKRIFDIAEKKTSKDFEPLSDVLERGFLEIERLFNNRG
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCC
EITGVGSGFSDLDSKTSGFQKGDMILIAARPSMGKTTFALNIAEHAALREGKSVVVFSLE
CEEECCCCHHHHCHHHCCCCCCCEEEEEECCCCCCCEEEEEHHHHHHHHCCCEEEEEEEC
MSKEQLAYKLLCSEANVDMLRLRTGELDDKDWENIARATGPLSKAKVYIDDTAGVSVMEM
CCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHH
RSKCRRLKMEYGIDLIVIDYLQLMSGSTSSDNRQQEVSEISRSIKALAKEMECPVIALSQ
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LSRAPEQRADHRPMLSDLRESGSIEQDADIVMFLYRDEYYDKETEDKNIAECIIAKQRNG
HHCCHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEEEHHHCCCCCCCCHHHHHHHHHCCCC
PVGTAKLAWLGQFSKFGNLDVIHKE
CCCHHHHHHHHHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 1312531 [H]