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The map label for this gene is apu [H]

Identifier: 187932508

GI number: 187932508

Start: 3596828

End: 3598642

Strand: Reverse

Name: apu [H]

Synonym: CLL_A3414

Alternate gene names: 187932508

Gene position: 3598642-3596828 (Counterclockwise)

Preceding gene: 187933573

Following gene: 187935023

Centisome position: 94.69

GC content: 25.34

Gene sequence:

>1815_bases
ATGGATGAAATAAAAGTGATATACAATTCTAGAGATAAGAATTTCAAGAAGCCTTTCGGGGCAGTTGAAATTGGTCAAGT
TATAAAATTATCGATTATAGTTAATAAAGATTTATTAGTAGCTCTTGAATTAACTGATTTCAATAATGAGAATAGCTTAT
TAGAAATGCAAAAAGAATACTTAAATGATGGAAATTATAAATACTCAGTAGAAATAGATACATCTAATAAATCAGGACTA
CTAAGATATTATTTTATTTTAATAGATGGTTATAAGAGAATTTATTATGGAAATAATGATGAAAGATTAGGCGGAGAAGG
ACAAGTATATAATAATGATCCTTTGCCATATGAAGTAACTGTATATGAAAAAATTGAAGTACCAAAATGGTATAAAGATG
GTGTTATATATCAGATATTTGTTGATAGATTTTGTAATGGAAATGAAGATAACAGCATAAATAAACCAAAGAAAAATTCT
TTTTTATATGCTACATGGGATGATGATCCAATGTATATAAAAGATAATATGGGAAGAATTTTGAGATGGGATTTTTATGG
TGGAAATCTAAAGGGAATAATAAAAAAGTTAGATTATATAAAATCTTTAGGTGCTAATATCATATATCTAAGTCCAATAT
TTAAATCTTCTAGTTGTCACAAATACGATGTTGGTGATTATGAAATTATAGATGAAATGTTTGGAACTAACGAAGAATTT
TCAAAATTATGTAATATAGCACACAGTAAAGGAATAAGAATTATATTAGACGGAGTATTCAGCAATACTGGATCTGATAG
TAGATATTTTAATAAGTATGGAAATTATGATGAAGTTGGCGCATATCAATCTCCTAATTCAAAGTATTACAATTGGTATA
AATTCATAACTTATCCTTATCAATATGAATCTTGGTGGGGAATAGATAATAGACCTAATGTAAATGAACTTGAAGAGAGT
TATCTAGATTATATCATAAATAAAAAGGGATCTATAATTGAAAAATGGATGAATTTGGGTGCGAGTGGATGGAGATTAAA
CGTTGCTGATGAATTACCAGATAAATTTATAGAGATTTTTAAAAAAAGAATGAAAGAAATTAATAATGATAGTGTGCTTA
TAGGAGAAGTTTGGGAGGATGCTTCTAATAAAATAAGTTATTCAAAACGAAGAAAATATTTGTTTGGCAAAGAATTAGAT
TCAGTCACTAATTATCCATTAAGAGAATATTTAATAAATTTTGTTAAAGGGTATATAACTTCTGAGAAACTAAAAAAAAG
AATAATGTCTTTATATGAGAATTATCCAAGAGAAATTTTTAATTCTAATATGAATGTTATAGGTACACATGATACTGAAA
GAATTTTAACAATATTAGATGGAAATTTATATTTACTTAAATTAATAATAGTTTTTCAAATGACATTACCAGGGGTACCG
GTAATTTATTATGGAGATGAAGCTGGGTTAAATGGTGGAAAAGATCCTGAAAACAGAAAGGCTTATCCATGGAATAATGA
AAATCAAGAGATATTAAAATTTTACTCTAAGATAATAAATATAAGAAAAAAAGAAGAAGTTTTAAGAAAAGGTAATTTAA
AGATATTTGATATGGATGTGAACATATGTGTATTAAAAAGAACATATGAAGGAAAAAACATAATAGTAGTATTAAATAAT
TCAGGATTGCATAAAAACTTAAATAAATTTGAATTTGAAGGTGTATATAGCGAATTATTTAGTAATAAAACTATAGATTT
TGATAAGGCTGATATCAACTTATCACCATATAATTTTTTAATATTATCTAAATAA

Upstream 100 bases:

>100_bases
TAGTTGTGATATTAGTAGGTTCCAATGAAGAAATAGCTCATGAAATAAACTAACATAATGACTAGTTATTTATTTAATGC
CTTATAGGAAGGACTAATTT

Downstream 100 bases:

>100_bases
ATATGTAAATAAATATGTAAATAAAAATGGATATTTTGTTTAAAGAAGATTTATAAGAGTTATGTCGTGAATATATGATA
ATTTAAGGGGAAAAAGAGAG

Product: amylopullulanase

Products: NA

Alternate protein names: Alpha-amylase/pullulanase; Alpha-amylase; 1,4-alpha-D-glucan glucanohydrolase; Pullulanase; 1,4-alpha-D-glucan glucanohydrolase; Alpha-dextrin endo-1,6-alpha-glucosidase [H]

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MDEIKVIYNSRDKNFKKPFGAVEIGQVIKLSIIVNKDLLVALELTDFNNENSLLEMQKEYLNDGNYKYSVEIDTSNKSGL
LRYYFILIDGYKRIYYGNNDERLGGEGQVYNNDPLPYEVTVYEKIEVPKWYKDGVIYQIFVDRFCNGNEDNSINKPKKNS
FLYATWDDDPMYIKDNMGRILRWDFYGGNLKGIIKKLDYIKSLGANIIYLSPIFKSSSCHKYDVGDYEIIDEMFGTNEEF
SKLCNIAHSKGIRIILDGVFSNTGSDSRYFNKYGNYDEVGAYQSPNSKYYNWYKFITYPYQYESWWGIDNRPNVNELEES
YLDYIINKKGSIIEKWMNLGASGWRLNVADELPDKFIEIFKKRMKEINNDSVLIGEVWEDASNKISYSKRRKYLFGKELD
SVTNYPLREYLINFVKGYITSEKLKKRIMSLYENYPREIFNSNMNVIGTHDTERILTILDGNLYLLKLIIVFQMTLPGVP
VIYYGDEAGLNGGKDPENRKAYPWNNENQEILKFYSKIINIRKKEEVLRKGNLKIFDMDVNICVLKRTYEGKNIIVVLNN
SGLHKNLNKFEFEGVYSELFSNKTIDFDKADINLSPYNFLILSK

Sequences:

>Translated_604_residues
MDEIKVIYNSRDKNFKKPFGAVEIGQVIKLSIIVNKDLLVALELTDFNNENSLLEMQKEYLNDGNYKYSVEIDTSNKSGL
LRYYFILIDGYKRIYYGNNDERLGGEGQVYNNDPLPYEVTVYEKIEVPKWYKDGVIYQIFVDRFCNGNEDNSINKPKKNS
FLYATWDDDPMYIKDNMGRILRWDFYGGNLKGIIKKLDYIKSLGANIIYLSPIFKSSSCHKYDVGDYEIIDEMFGTNEEF
SKLCNIAHSKGIRIILDGVFSNTGSDSRYFNKYGNYDEVGAYQSPNSKYYNWYKFITYPYQYESWWGIDNRPNVNELEES
YLDYIINKKGSIIEKWMNLGASGWRLNVADELPDKFIEIFKKRMKEINNDSVLIGEVWEDASNKISYSKRRKYLFGKELD
SVTNYPLREYLINFVKGYITSEKLKKRIMSLYENYPREIFNSNMNVIGTHDTERILTILDGNLYLLKLIIVFQMTLPGVP
VIYYGDEAGLNGGKDPENRKAYPWNNENQEILKFYSKIINIRKKEEVLRKGNLKIFDMDVNICVLKRTYEGKNIIVVLNN
SGLHKNLNKFEFEGVYSELFSNKTIDFDKADINLSPYNFLILSK
>Mature_604_residues
MDEIKVIYNSRDKNFKKPFGAVEIGQVIKLSIIVNKDLLVALELTDFNNENSLLEMQKEYLNDGNYKYSVEIDTSNKSGL
LRYYFILIDGYKRIYYGNNDERLGGEGQVYNNDPLPYEVTVYEKIEVPKWYKDGVIYQIFVDRFCNGNEDNSINKPKKNS
FLYATWDDDPMYIKDNMGRILRWDFYGGNLKGIIKKLDYIKSLGANIIYLSPIFKSSSCHKYDVGDYEIIDEMFGTNEEF
SKLCNIAHSKGIRIILDGVFSNTGSDSRYFNKYGNYDEVGAYQSPNSKYYNWYKFITYPYQYESWWGIDNRPNVNELEES
YLDYIINKKGSIIEKWMNLGASGWRLNVADELPDKFIEIFKKRMKEINNDSVLIGEVWEDASNKISYSKRRKYLFGKELD
SVTNYPLREYLINFVKGYITSEKLKKRIMSLYENYPREIFNSNMNVIGTHDTERILTILDGNLYLLKLIIVFQMTLPGVP
VIYYGDEAGLNGGKDPENRKAYPWNNENQEILKFYSKIINIRKKEEVLRKGNLKIFDMDVNICVLKRTYEGKNIIVVLNN
SGLHKNLNKFEFEGVYSELFSNKTIDFDKADINLSPYNFLILSK

Specific function: May Play A Role In Regulating The Intracellular Level Of Maltotriose. Cleaves Glucose From The Reducing End Of Maltotriose And Longer Maltodextrins With A Chain Length Of Up To 7 Glucose Units. [C]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 fibronectin type-III domains [H]

Homologues:

Organism=Escherichia coli, GI1786604, Length=554, Percent_Identity=29.2418772563177, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1790687, Length=590, Percent_Identity=21.864406779661, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1789995, Length=149, Percent_Identity=27.5167785234899, Blast_Score=66, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6322245, Length=147, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6324416, Length=144, Percent_Identity=30.5555555555556, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6321726, Length=144, Percent_Identity=29.8611111111111, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322241, Length=144, Percent_Identity=29.8611111111111, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322021, Length=144, Percent_Identity=29.8611111111111, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24586587, Length=377, Percent_Identity=23.8726790450928, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI45549022, Length=507, Percent_Identity=20.9072978303748, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24586599, Length=555, Percent_Identity=20.9009009009009, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24583749, Length=501, Percent_Identity=21.5568862275449, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24583747, Length=501, Percent_Identity=21.5568862275449, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24586591, Length=84, Percent_Identity=35.7142857142857, Blast_Score=66, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006048
- InterPro:   IPR013784
- InterPro:   IPR008957
- InterPro:   IPR003961
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR002044
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N; PF00686 CBM_20; PF00041 fn3 [H]

EC number: =3.2.1.1; =3.2.1.41 [H]

Molecular weight: Translated: 70758; Mature: 70758

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEIKVIYNSRDKNFKKPFGAVEIGQVIKLSIIVNKDLLVALELTDFNNENSLLEMQKEY
CCCEEEEECCCCCCCCCCCCCEECCCEEEEEEEECCCEEEEEEEECCCCCCHHHHHHHHH
LNDGNYKYSVEIDTSNKSGLLRYYFILIDGYKRIYYGNNDERLGGEGQVYNNDPLPYEVT
CCCCCEEEEEEEECCCCCCEEEEEEEEEECCEEEEECCCCCCCCCCCEEECCCCCCEEEE
VYEKIEVPKWYKDGVIYQIFVDRFCNGNEDNSINKPKKNSFLYATWDDDPMYIKDNMGRI
EEEEECCCCHHHCCEEEEEEHHHHCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCE
LRWDFYGGNLKGIIKKLDYIKSLGANIIYLSPIFKSSSCHKYDVGDYEIIDEMFGTNEEF
EEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCHHH
SKLCNIAHSKGIRIILDGVFSNTGSDSRYFNKYGNYDEVGAYQSPNSKYYNWYKFITYPY
HHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEECE
QYESWWGIDNRPNVNELEESYLDYIINKKGSIIEKWMNLGASGWRLNVADELPDKFIEIF
EECCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECHHHCCHHHHHHH
KKRMKEINNDSVLIGEVWEDASNKISYSKRRKYLFGKELDSVTNYPLREYLINFVKGYIT
HHHHHHCCCCCEEEEEHHCCCCCCCCHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHC
SEKLKKRIMSLYENYPREIFNSNMNVIGTHDTERILTILDGNLYLLKLIIVFQMTLPGVP
HHHHHHHHHHHHHHCCHHHHCCCCCEEECCCHHEEEEEECCCHHHHHHHHHHHHHCCCCE
VIYYGDEAGLNGGKDPENRKAYPWNNENQEILKFYSKIINIRKKEEVLRKGNLKIFDMDV
EEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECC
NICVLKRTYEGKNIIVVLNNSGLHKNLNKFEFEGVYSELFSNKTIDFDKADINLSPYNFL
EEEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
ILSK
EEEC
>Mature Secondary Structure
MDEIKVIYNSRDKNFKKPFGAVEIGQVIKLSIIVNKDLLVALELTDFNNENSLLEMQKEY
CCCEEEEECCCCCCCCCCCCCEECCCEEEEEEEECCCEEEEEEEECCCCCCHHHHHHHHH
LNDGNYKYSVEIDTSNKSGLLRYYFILIDGYKRIYYGNNDERLGGEGQVYNNDPLPYEVT
CCCCCEEEEEEEECCCCCCEEEEEEEEEECCEEEEECCCCCCCCCCCEEECCCCCCEEEE
VYEKIEVPKWYKDGVIYQIFVDRFCNGNEDNSINKPKKNSFLYATWDDDPMYIKDNMGRI
EEEEECCCCHHHCCEEEEEEHHHHCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCE
LRWDFYGGNLKGIIKKLDYIKSLGANIIYLSPIFKSSSCHKYDVGDYEIIDEMFGTNEEF
EEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCHHH
SKLCNIAHSKGIRIILDGVFSNTGSDSRYFNKYGNYDEVGAYQSPNSKYYNWYKFITYPY
HHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEECE
QYESWWGIDNRPNVNELEESYLDYIINKKGSIIEKWMNLGASGWRLNVADELPDKFIEIF
EECCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECHHHCCHHHHHHH
KKRMKEINNDSVLIGEVWEDASNKISYSKRRKYLFGKELDSVTNYPLREYLINFVKGYIT
HHHHHHCCCCCEEEEEHHCCCCCCCCHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHC
SEKLKKRIMSLYENYPREIFNSNMNVIGTHDTERILTILDGNLYLLKLIIVFQMTLPGVP
HHHHHHHHHHHHHHCCHHHHCCCCCEEECCCHHEEEEEECCCHHHHHHHHHHHHHCCCCE
VIYYGDEAGLNGGKDPENRKAYPWNNENQEILKFYSKIINIRKKEEVLRKGNLKIFDMDV
EEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECC
NICVLKRTYEGKNIIVVLNNSGLHKNLNKFEFEGVYSELFSNKTIDFDKADINLSPYNFL
EEEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
ILSK
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2391488; 3260488; 1409594 [H]