| Definition | Clostridium botulinum B str. Eklund 17B, complete genome. |
|---|---|
| Accession | NC_010674 |
| Length | 3,800,327 |
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The map label for this gene is pheT [H]
Identifier: 187932405
GI number: 187932405
Start: 2564275
End: 2566650
Strand: Reverse
Name: pheT [H]
Synonym: CLL_A2472
Alternate gene names: 187932405
Gene position: 2566650-2564275 (Counterclockwise)
Preceding gene: 187935783
Following gene: 187933376
Centisome position: 67.54
GC content: 30.01
Gene sequence:
>2376_bases ATGAAAGTACCATTTAGTTGGTTACAAGATTATGTTGATATAAATGTTAGTCCTAAAGAATTAGGAGATAAATTAACTTT AACAGGATCACAATTAGAAGAATTAATAATTCAAGGTGATGTTATAGATAAAGTTGTTACAGGAAAAATTACTGAAATAG AAAAACATCCCGATGCTGATAAACTAAGCATCTGTCAAGTGGATATTGGAACTGAAACTATTCAAATAGTTACGGCTGCA ACAAATATGAAAGAGCAAGATGTAGTACCAGTAGCTTTACATGGTTCAACATTGGCAGATGGAACTAAGATTAAAAAAGG TAAGCTTAGAGGGGTACCATCAAATGGTATGTTCTGTTCAGAAGAGGAATTAGGTACAGCTGGAGATGAACCAGTACATG GTTTGATGATATTACCTACTGATACAGAATTAGGAGTAGAAATAAAAGAATTATTAAAATTAAACAAAGCTATATTAGAT TTTGAAATAACTTCTAATAGACCTGATTGTTTAAGTATAGTTGGTATGGCTAGAGAAACAGCAGCTGCACTTAGGACTAC TTATAAGATGCCTAATTTAGAATATAAAGTTTCTGGAAATGGAAATGTAGAATCAGAATTAAATGTTGAAGTTAAAGATG ACTTATGCTTGAGATATATGGCTAGAAAAGTTAAAAATGTAAAAGTAAAACCATCACCAGGCTGGATGCAAGAAAGACTT CTTGAAGCAGGAATAAGACCCATAGACAATATAGTTGATATTACAAATTTTATAATGCTTGAATTAGGTCAACCAATGCA TGCTTATGATGCTAGAGAAATATCTACTAATAAGATAGTTGTTGAAAGAGCTGAAAGTGGAGAAAAATTCACTACTTTAG ATGAAGTTGAAAGACAATTAGATGACTCTATGCTTTGCATAAAGGATGACAATAAGATTGTTGGGTTAGCTGGAATAATG GGTGGATTAAATTCAGAAATAAAAGAAGATACAACTGAAGTTATTTTTGAATCTGCAAATTTTGATGGTACGAACATAAG AGTTAATTCTAAAAAATTAAACTTAAGAAGTGAAGCTTCTGGAAGATTTGAAAAAGATATAGATCCTAACTTAGCTAAAC TTGCAATAGATAGAGCATGCGCTTTGATTTGTGAATTAGGTGCTGGTGAAGTTATAGAAGGAACAATAGATATTTATAAC AAAAAGAAAGAACCAGGAAAAGTAATTGTTGATTCTAATTGGGTTAATAAATTCCTTGGAACAAATCTTTCTAAAGAAGA AATGAAAAAATGTTTAGATAGCGTTGATTTATTTACAGAAATTGATGGAGATAATTTAAATGTAACAGCTCCAACATTTA GAATAGACATTGCTATTAAAGAAGATATAGCTGAAGAAATAGCTAGAATACATGGATATGATGTTATTCCTGCTACAATC TTTAGTGTTGCTACATCAAGAGAACCTAAATACAGAAATAGATTATTAGATGATAAAGTAGTTATGCTTGCTACAGGTAG CGGATTAAATCAATCTATAAGCTATTCATTTGTATCACCAAGGGTATTTGATAAAATTAATGTACCAGAAGATAGCGAAC TTAGAAATGTAGTTAAAATAAAAAATCCTTTAGGCGAAGATTATAGTGTTATGAGAACTACTACTATACCTTCAATGATG GAATGCTTAGGAAGAAACTATTCAAGAAATAATGATTATGTTAGACTATTTGAAATGGCTAAAATATATATTAAAAATGA AGATGAAACTAAGATTCCAACTGAAAGAAATATATTAACTATTGGTATGTATGGGGATTGTGATTATTTAGATCTTAAAG GCGCAGTTGAAAACATAATTGATGGTCTTGGAATAAAGAATTCTAAGTATGAAAGAGAAAGTGAAAATGTAAGCTATCAT CCAGGAAAAACTGCAAAACTTGTTATAGGCAAAAATGTAGTTGGTACTTTAGGTGAAGTTCATTTAGATGTAACTGAAAA CTATGGAATTGATGTACCTTGCTTTATTGCAGAACTTAATTTAGATGCATTATATGAAAGTGCTGATATGGATAGAAAGT ATAAAGCATTACCTAAATTCCCAGCTGTTACAAGAGATATTGCATTACTTGTAGAAGATAGTATATTAGTTCAAGAAATT GAAGAATGTATAAGAAAAGCTGGTGGAAATTTAGTAGAAAAAGTACAATTATTTGATATCTACAAAGGAAAGCAAATTCC AGATGGCAAAAAGAGCATAGCTTATGCTATTGCTTATAGAGCTGATAAAACATTAACTGATAAAGAAGTTAATAAAGTAC ATGATAAGATATTAAGATCATTAGAATATAAATTAGGTGCTACTTTAAGAGACTAA
Upstream 100 bases:
>100_bases TAACAATATTACTTAATTATATAGCTTGAAAGACATAAAAATACAAGTTCTAAAGAAATTGATAATTTGAGAACTTAAGG AAAAATGAGGAGGAAAAAAT
Downstream 100 bases:
>100_bases TTTTATTGAAAATACAGATGACTAGATTTTGTAAACTTAAATCTAGTCATTTTTTTTATGTATAGGAAAGTTTTGATAAG ATGAATTAAAGTAAAATAAA
Product: phenylalanyl-tRNA synthetase subunit beta
Products: NA
Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]
Number of amino acids: Translated: 791; Mature: 791
Protein sequence:
>791_residues MKVPFSWLQDYVDINVSPKELGDKLTLTGSQLEELIIQGDVIDKVVTGKITEIEKHPDADKLSICQVDIGTETIQIVTAA TNMKEQDVVPVALHGSTLADGTKIKKGKLRGVPSNGMFCSEEELGTAGDEPVHGLMILPTDTELGVEIKELLKLNKAILD FEITSNRPDCLSIVGMARETAAALRTTYKMPNLEYKVSGNGNVESELNVEVKDDLCLRYMARKVKNVKVKPSPGWMQERL LEAGIRPIDNIVDITNFIMLELGQPMHAYDAREISTNKIVVERAESGEKFTTLDEVERQLDDSMLCIKDDNKIVGLAGIM GGLNSEIKEDTTEVIFESANFDGTNIRVNSKKLNLRSEASGRFEKDIDPNLAKLAIDRACALICELGAGEVIEGTIDIYN KKKEPGKVIVDSNWVNKFLGTNLSKEEMKKCLDSVDLFTEIDGDNLNVTAPTFRIDIAIKEDIAEEIARIHGYDVIPATI FSVATSREPKYRNRLLDDKVVMLATGSGLNQSISYSFVSPRVFDKINVPEDSELRNVVKIKNPLGEDYSVMRTTTIPSMM ECLGRNYSRNNDYVRLFEMAKIYIKNEDETKIPTERNILTIGMYGDCDYLDLKGAVENIIDGLGIKNSKYERESENVSYH PGKTAKLVIGKNVVGTLGEVHLDVTENYGIDVPCFIAELNLDALYESADMDRKYKALPKFPAVTRDIALLVEDSILVQEI EECIRKAGGNLVEKVQLFDIYKGKQIPDGKKSIAYAIAYRADKTLTDKEVNKVHDKILRSLEYKLGATLRD
Sequences:
>Translated_791_residues MKVPFSWLQDYVDINVSPKELGDKLTLTGSQLEELIIQGDVIDKVVTGKITEIEKHPDADKLSICQVDIGTETIQIVTAA TNMKEQDVVPVALHGSTLADGTKIKKGKLRGVPSNGMFCSEEELGTAGDEPVHGLMILPTDTELGVEIKELLKLNKAILD FEITSNRPDCLSIVGMARETAAALRTTYKMPNLEYKVSGNGNVESELNVEVKDDLCLRYMARKVKNVKVKPSPGWMQERL LEAGIRPIDNIVDITNFIMLELGQPMHAYDAREISTNKIVVERAESGEKFTTLDEVERQLDDSMLCIKDDNKIVGLAGIM GGLNSEIKEDTTEVIFESANFDGTNIRVNSKKLNLRSEASGRFEKDIDPNLAKLAIDRACALICELGAGEVIEGTIDIYN KKKEPGKVIVDSNWVNKFLGTNLSKEEMKKCLDSVDLFTEIDGDNLNVTAPTFRIDIAIKEDIAEEIARIHGYDVIPATI FSVATSREPKYRNRLLDDKVVMLATGSGLNQSISYSFVSPRVFDKINVPEDSELRNVVKIKNPLGEDYSVMRTTTIPSMM ECLGRNYSRNNDYVRLFEMAKIYIKNEDETKIPTERNILTIGMYGDCDYLDLKGAVENIIDGLGIKNSKYERESENVSYH PGKTAKLVIGKNVVGTLGEVHLDVTENYGIDVPCFIAELNLDALYESADMDRKYKALPKFPAVTRDIALLVEDSILVQEI EECIRKAGGNLVEKVQLFDIYKGKQIPDGKKSIAYAIAYRADKTLTDKEVNKVHDKILRSLEYKLGATLRD >Mature_791_residues MKVPFSWLQDYVDINVSPKELGDKLTLTGSQLEELIIQGDVIDKVVTGKITEIEKHPDADKLSICQVDIGTETIQIVTAA TNMKEQDVVPVALHGSTLADGTKIKKGKLRGVPSNGMFCSEEELGTAGDEPVHGLMILPTDTELGVEIKELLKLNKAILD FEITSNRPDCLSIVGMARETAAALRTTYKMPNLEYKVSGNGNVESELNVEVKDDLCLRYMARKVKNVKVKPSPGWMQERL LEAGIRPIDNIVDITNFIMLELGQPMHAYDAREISTNKIVVERAESGEKFTTLDEVERQLDDSMLCIKDDNKIVGLAGIM GGLNSEIKEDTTEVIFESANFDGTNIRVNSKKLNLRSEASGRFEKDIDPNLAKLAIDRACALICELGAGEVIEGTIDIYN KKKEPGKVIVDSNWVNKFLGTNLSKEEMKKCLDSVDLFTEIDGDNLNVTAPTFRIDIAIKEDIAEEIARIHGYDVIPATI FSVATSREPKYRNRLLDDKVVMLATGSGLNQSISYSFVSPRVFDKINVPEDSELRNVVKIKNPLGEDYSVMRTTTIPSMM ECLGRNYSRNNDYVRLFEMAKIYIKNEDETKIPTERNILTIGMYGDCDYLDLKGAVENIIDGLGIKNSKYERESENVSYH PGKTAKLVIGKNVVGTLGEVHLDVTENYGIDVPCFIAELNLDALYESADMDRKYKALPKFPAVTRDIALLVEDSILVQEI EECIRKAGGNLVEKVQLFDIYKGKQIPDGKKSIAYAIAYRADKTLTDKEVNKVHDKILRSLEYKLGATLRD
Specific function: Unknown
COG id: COG0072
COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 tRNA-binding domain [H]
Homologues:
Organism=Homo sapiens, GI124028525, Length=287, Percent_Identity=24.390243902439, Blast_Score=83, Evalue=1e-15, Organism=Escherichia coli, GI1788006, Length=818, Percent_Identity=30.9290953545232, Blast_Score=377, Evalue=1e-105, Organism=Caenorhabditis elegans, GI17533403, Length=290, Percent_Identity=25.1724137931034, Blast_Score=90, Evalue=4e-18, Organism=Drosophila melanogaster, GI21355211, Length=365, Percent_Identity=21.3698630136986, Blast_Score=79, Evalue=1e-14,
Paralogues:
None
Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR005146 - InterPro: IPR005147 - InterPro: IPR009061 - InterPro: IPR012340 - InterPro: IPR016027 - InterPro: IPR004532 - InterPro: IPR020825 - InterPro: IPR005121 - InterPro: IPR002547 [H]
Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]
EC number: =6.1.1.20 [H]
Molecular weight: Translated: 88107; Mature: 88107
Theoretical pI: Translated: 4.73; Mature: 4.73
Prosite motif: PS50886 TRBD
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.5 %Cys (Translated Protein) 2.5 %Met (Translated Protein) 4.0 %Cys+Met (Translated Protein) 1.5 %Cys (Mature Protein) 2.5 %Met (Mature Protein) 4.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKVPFSWLQDYVDINVSPKELGDKLTLTGSQLEELIIQGDVIDKVVTGKITEIEKHPDAD CCCCHHHHHHHHCCCCCHHHHCCEEEECHHHHHHHHHCCHHHHHHHCCCCEECCCCCCCC KLSICQVDIGTETIQIVTAATNMKEQDVVPVALHGSTLADGTKIKKGKLRGVPSNGMFCS CEEEEEEECCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCCEECCCCCCCCCCCCCEEC EEELGTAGDEPVHGLMILPTDTELGVEIKELLKLNKAILDFEITSNRPDCLSIVGMARET HHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHH AAALRTTYKMPNLEYKVSGNGNVESELNVEVKDDLCLRYMARKVKNVKVKPSPGWMQERL HHHHHHHHCCCCEEEEECCCCCCCCEEEEEECHHHHHHHHHHHHHCCEECCCCCHHHHHH LEAGIRPIDNIVDITNFIMLELGQPMHAYDAREISTNKIVVERAESGEKFTTLDEVERQL HHHCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHC DDSMLCIKDDNKIVGLAGIMGGLNSEIKEDTTEVIFESANFDGTNIRVNSKKLNLRSEAS CCCEEEEECCCCEEEHHHHHCCCCHHHHHHHHHHHEECCCCCCCEEEEECCEECCCCCCC GRFEKDIDPNLAKLAIDRACALICELGAGEVIEGTIDIYNKKKEPGKVIVDSNWVNKFLG CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCEECCCCCCCCEEEECCHHHHHHHC TNLSKEEMKKCLDSVDLFTEIDGDNLNVTAPTFRIDIAIKEDIAEEIARIHGYDVIPATI CCCCHHHHHHHHHHHHHHEEECCCCEEEECCEEEEEEEEHHHHHHHHHHHCCCCCHHHHH FSVATSREPKYRNRLLDDKVVMLATGSGLNQSISYSFVSPRVFDKINVPEDSELRNVVKI HHHHCCCCCHHHHHCCCCCEEEEEECCCCCCCCEEEECCCHHHHCCCCCCCHHHHHHHHH KNPLGEDYSVMRTTTIPSMMECLGRNYSRNNDYVRLFEMAKIYIKNEDETKIPTERNILT CCCCCCCCEEEEECCCHHHHHHHCCCCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCEEE IGMYGDCDYLDLKGAVENIIDGLGIKNSKYERESENVSYHPGKTAKLVIGKNVVGTLGEV EEECCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCEEEEECCCHHHCCCEE HLDVTENYGIDVPCFIAELNLDALYESADMDRKYKALPKFPAVTRDIALLVEDSILVQEI EEEEECCCCCCCEEEEEECCHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHCCHHHHHHH EECIRKAGGNLVEKVQLFDIYKGKQIPDGKKSIAYAIAYRADKTLTDKEVNKVHDKILRS HHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHEEEEEEEECCCCCCHHHHHHHHHHHHHH LEYKLGATLRD HHHHHCCCCCC >Mature Secondary Structure MKVPFSWLQDYVDINVSPKELGDKLTLTGSQLEELIIQGDVIDKVVTGKITEIEKHPDAD CCCCHHHHHHHHCCCCCHHHHCCEEEECHHHHHHHHHCCHHHHHHHCCCCEECCCCCCCC KLSICQVDIGTETIQIVTAATNMKEQDVVPVALHGSTLADGTKIKKGKLRGVPSNGMFCS CEEEEEEECCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCCEECCCCCCCCCCCCCEEC EEELGTAGDEPVHGLMILPTDTELGVEIKELLKLNKAILDFEITSNRPDCLSIVGMARET HHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHH AAALRTTYKMPNLEYKVSGNGNVESELNVEVKDDLCLRYMARKVKNVKVKPSPGWMQERL HHHHHHHHCCCCEEEEECCCCCCCCEEEEEECHHHHHHHHHHHHHCCEECCCCCHHHHHH LEAGIRPIDNIVDITNFIMLELGQPMHAYDAREISTNKIVVERAESGEKFTTLDEVERQL HHHCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHC DDSMLCIKDDNKIVGLAGIMGGLNSEIKEDTTEVIFESANFDGTNIRVNSKKLNLRSEAS CCCEEEEECCCCEEEHHHHHCCCCHHHHHHHHHHHEECCCCCCCEEEEECCEECCCCCCC GRFEKDIDPNLAKLAIDRACALICELGAGEVIEGTIDIYNKKKEPGKVIVDSNWVNKFLG CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCEECCCCCCCCEEEECCHHHHHHHC TNLSKEEMKKCLDSVDLFTEIDGDNLNVTAPTFRIDIAIKEDIAEEIARIHGYDVIPATI CCCCHHHHHHHHHHHHHHEEECCCCEEEECCEEEEEEEEHHHHHHHHHHHCCCCCHHHHH FSVATSREPKYRNRLLDDKVVMLATGSGLNQSISYSFVSPRVFDKINVPEDSELRNVVKI HHHHCCCCCHHHHHCCCCCEEEEEECCCCCCCCEEEECCCHHHHCCCCCCCHHHHHHHHH KNPLGEDYSVMRTTTIPSMMECLGRNYSRNNDYVRLFEMAKIYIKNEDETKIPTERNILT CCCCCCCCEEEEECCCHHHHHHHCCCCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCEEE IGMYGDCDYLDLKGAVENIIDGLGIKNSKYERESENVSYHPGKTAKLVIGKNVVGTLGEV EEECCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCEEEEECCCHHHCCCEE HLDVTENYGIDVPCFIAELNLDALYESADMDRKYKALPKFPAVTRDIALLVEDSILVQEI EEEEECCCCCCCEEEEEECCHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHCCHHHHHHH EECIRKAGGNLVEKVQLFDIYKGKQIPDGKKSIAYAIAYRADKTLTDKEVNKVHDKILRS HHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHEEEEEEEECCCCCCHHHHHHHHHHHHHH LEYKLGATLRD HHHHHCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11792842 [H]