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The map label for this gene is metF [H]

Identifier: 187932404

GI number: 187932404

Start: 2930727

End: 2931614

Strand: Reverse

Name: metF [H]

Synonym: CLL_A2856

Alternate gene names: 187932404

Gene position: 2931614-2930727 (Counterclockwise)

Preceding gene: 187934123

Following gene: 187933093

Centisome position: 77.14

GC content: 28.72

Gene sequence:

>888_bases
ATGAATATTAAAAATTTATTCAGAGACAAAAAGGTGGTTTTTTCATTTGAAATTTTCCCACCTAAAACAACATCATCCAT
ACAAACTATTTATAATACATTAGATGATTTAAAAGGATTAAATCCAGATTATATAAGTGTTACCTATGGGGCAGGTGGCA
GTGTTAAAAACAATAAGACTATTGAGCTAACTTCTCTTATAAAGAATAAATATGGAATTGAAGCAGTATCACATTTAACT
TGTATTAGTTCAACTAAAAGTGATATAGAATATTATTTAAGAGAATTTGAAAAGAATAATATAGAAAATATCCTTGCATT
AAGAGGGGATATTCCTATAGGAAGTAAGATTACAGGAGAATTAAATCATGCTAACGAATTAATTAAGCATATAAGTGATA
ATGGACAATTTAATATAGCTGCAGCATGTTATCCAGAAGGCCATATAGAGCATAAAGGGTTGTATAGAGAAATTGAGAGC
ATGAAGAGGAAAGAAGAGGCAGGAGCATCTTATTTTATATCTCAGTTGTTTTTTGATAACAATTTATATTATAATTTTCA
AGACGAGGTTCGTGCTGCTAATATAAATTTACCTATTGAAGCTGGAATTATGCCTGTAACAAATAAAAGACAAATTGAAA
GAATTTTATCTTTAAGTGGAGCATCACTTCCAGATAAATTTAAAAGAATAATGGAAAGATATGAGCATAATCCAGAAGCT
TTAAGAGATGCGGGTATTGCTTATGCAGTTGAACAAATCGTAGACCTAATTTCTACAGGTGTGGATGGAATACATTTATA
TGTTATGAATAATCCATATGTAGCTAAAAGAATAAGTCAAAGTATAGAAAATATTCTATTAACAGTAAATAAAAATCAAT
TCGTTTAG

Upstream 100 bases:

>100_bases
AGAAATGAATCCTAAAGAATAAAAAATAATCATATTATGCCGCATCATTATCCCTACATTGCGTTTGGAAAGGGTGTATG
GCTAAAAAGGGGGCAAAAAA

Downstream 100 bases:

>100_bases
GAGGATACAATAATGGAAAAAGTAGAAAGTTTTGAATTAGATCACAGAAAGGTTAAAGCACCATACATAAGAAAATGTTG
TTTGCTTGATGGTAAGCTTG

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MNIKNLFRDKKVVFSFEIFPPKTTSSIQTIYNTLDDLKGLNPDYISVTYGAGGSVKNNKTIELTSLIKNKYGIEAVSHLT
CISSTKSDIEYYLREFEKNNIENILALRGDIPIGSKITGELNHANELIKHISDNGQFNIAAACYPEGHIEHKGLYREIES
MKRKEEAGASYFISQLFFDNNLYYNFQDEVRAANINLPIEAGIMPVTNKRQIERILSLSGASLPDKFKRIMERYEHNPEA
LRDAGIAYAVEQIVDLISTGVDGIHLYVMNNPYVAKRISQSIENILLTVNKNQFV

Sequences:

>Translated_295_residues
MNIKNLFRDKKVVFSFEIFPPKTTSSIQTIYNTLDDLKGLNPDYISVTYGAGGSVKNNKTIELTSLIKNKYGIEAVSHLT
CISSTKSDIEYYLREFEKNNIENILALRGDIPIGSKITGELNHANELIKHISDNGQFNIAAACYPEGHIEHKGLYREIES
MKRKEEAGASYFISQLFFDNNLYYNFQDEVRAANINLPIEAGIMPVTNKRQIERILSLSGASLPDKFKRIMERYEHNPEA
LRDAGIAYAVEQIVDLISTGVDGIHLYVMNNPYVAKRISQSIENILLTVNKNQFV
>Mature_295_residues
MNIKNLFRDKKVVFSFEIFPPKTTSSIQTIYNTLDDLKGLNPDYISVTYGAGGSVKNNKTIELTSLIKNKYGIEAVSHLT
CISSTKSDIEYYLREFEKNNIENILALRGDIPIGSKITGELNHANELIKHISDNGQFNIAAACYPEGHIEHKGLYREIES
MKRKEEAGASYFISQLFFDNNLYYNFQDEVRAANINLPIEAGIMPVTNKRQIERILSLSGASLPDKFKRIMERYEHNPEA
LRDAGIAYAVEQIVDLISTGVDGIHLYVMNNPYVAKRISQSIENILLTVNKNQFV

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=284, Percent_Identity=32.7464788732394, Blast_Score=155, Evalue=6e-38,
Organism=Escherichia coli, GI1790377, Length=268, Percent_Identity=33.955223880597, Blast_Score=174, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI25147436, Length=290, Percent_Identity=28.6206896551724, Blast_Score=130, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI25147441, Length=290, Percent_Identity=28.6206896551724, Blast_Score=130, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6321313, Length=270, Percent_Identity=32.5925925925926, Blast_Score=146, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6325234, Length=302, Percent_Identity=31.4569536423841, Blast_Score=139, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 33392; Mature: 33392

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKNLFRDKKVVFSFEIFPPKTTSSIQTIYNTLDDLKGLNPDYISVTYGAGGSVKNNKT
CCHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCE
IELTSLIKNKYGIEAVSHLTCISSTKSDIEYYLREFEKNNIENILALRGDIPIGSKITGE
EEHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHEEECCCCCCCCCCCC
LNHANELIKHISDNGQFNIAAACYPEGHIEHKGLYREIESMKRKEEAGASYFISQLFFDN
HHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NLYYNFQDEVRAANINLPIEAGIMPVTNKRQIERILSLSGASLPDKFKRIMERYEHNPEA
CCEECCHHHEEEEECCCCHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHH
LRDAGIAYAVEQIVDLISTGVDGIHLYVMNNPYVAKRISQSIENILLTVNKNQFV
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHEEEEECCCCCC
>Mature Secondary Structure
MNIKNLFRDKKVVFSFEIFPPKTTSSIQTIYNTLDDLKGLNPDYISVTYGAGGSVKNNKT
CCHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCE
IELTSLIKNKYGIEAVSHLTCISSTKSDIEYYLREFEKNNIENILALRGDIPIGSKITGE
EEHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHEEECCCCCCCCCCCC
LNHANELIKHISDNGQFNIAAACYPEGHIEHKGLYREIESMKRKEEAGASYFISQLFFDN
HHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NLYYNFQDEVRAANINLPIEAGIMPVTNKRQIERILSLSGASLPDKFKRIMERYEHNPEA
CCEECCHHHEEEEECCCCHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHH
LRDAGIAYAVEQIVDLISTGVDGIHLYVMNNPYVAKRISQSIENILLTVNKNQFV
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]