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The map label for this gene is tgt

Identifier: 187918663

GI number: 187918663

Start: 868484

End: 869611

Strand: Direct

Name: tgt

Synonym: BH0809

Alternate gene names: 187918663

Gene position: 868484-869611 (Clockwise)

Preceding gene: 187918662

Following gene: 187918664

Centisome position: 94.16

GC content: 31.83

Gene sequence:

>1128_bases
ATGTTTAATATTATTAAAAATGATAAGAATTCTAATGCACGAATTGGTGTGCTTGAACTGCCTCATGGGAAGGTAACAAC
TCCATGTTTCATGCCAGTTGGTACCTTGGGCGTAATGAAAGCTTTAAAGCATGATGTGCTTGAGAAATTGGGATGTAATT
TGATGCTTGCAAATACTTATCATCTTTATTTAAGACCTGGCATTGATGTAATAAAAGAATATGGAAGTTTGCATAATTTT
ACAACTTGGAATAAAAATTTTTTAACAGATTCTGGGGGATTTCAAGTATTCTCTTTGTCTAATTTTAGAAAGATTGAAAC
TGAGGGAGTAGATTTTAAGTCTCATATTGATGGCTCTAGGCACTATTTTACACCTGAGAGTGTATTTAAGATGCAAGAAA
TTTTTGAAAGTGATATTATTATGGCACTTGATATTTGTAGTTCTTATGGTATTGATTACAGTGAGGCGAGTTTATATGCA
AATATTACAACCTCTTGGGCTCGTCGTACATTGCGTGCTTATGAGAATAGAAAAGAAGGATATGATGGTTTTTTATTCTT
AATAACGCAAGGTAATTTTTTTAAAGATTTGAGGAAAAGAAGTACTGAAGCAATTTTAGAATTAAATAGTCCTGGTATTG
CAATTGGTGGAATTTCTGTTGGAGAACCAAGAGATAAATATTTAGAAATTCTTGAATATAATTCTTCATTAATACCTAAA
GATAAGCCAAAGTACGTAATGGGAATTGGTACGCCGCACTATATATTGGATGCAATATATTATGGTATTGATATCTTTGA
TTGTGTTAATCCTACAAGAATTGCTAGACATGGTTCACTTTTAACTGATAATGGAATATTGCGCATTAAGAGGGCCGGGT
TTAATGTTGATACTTCTCCTATTGAGCAAGATTGTTCTTGCACTTTATGTACAAGGTATTCAAGAGGATATTTAAGACAT
TTAATCAAATCAGGAGAAACCCTTGGAGTTATGTTAGCTAGTGAGCATAACATTCATTATATGTTTAGACTTATTCAAAA
GGCTCGAAATGCAATTATGAATGATGATTTTACACAATTTAGAAAGCTTTATTTAAGCAAGTATGATGAAGGCAATTTTA
ATGAATAG

Upstream 100 bases:

>100_bases
GCCCATATTTGTTGCAAGTTCATTGCCAACTATTTTATTTATTATTATTAATTTTGTCGTTTATAATCTTGTATGTAAGT
AGTAGGTTTTTGGGAATTTT

Downstream 100 bases:

>100_bases
AGATATTATTTCAACAATTATTGTTATGGTTTCGATCTTTTTTTCACGTATTATGGGTTTTATTAAGATAAAGGTATTTT
CTTACTATTTTGGCGCAAAT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MFNIIKNDKNSNARIGVLELPHGKVTTPCFMPVGTLGVMKALKHDVLEKLGCNLMLANTYHLYLRPGIDVIKEYGSLHNF
TTWNKNFLTDSGGFQVFSLSNFRKIETEGVDFKSHIDGSRHYFTPESVFKMQEIFESDIIMALDICSSYGIDYSEASLYA
NITTSWARRTLRAYENRKEGYDGFLFLITQGNFFKDLRKRSTEAILELNSPGIAIGGISVGEPRDKYLEILEYNSSLIPK
DKPKYVMGIGTPHYILDAIYYGIDIFDCVNPTRIARHGSLLTDNGILRIKRAGFNVDTSPIEQDCSCTLCTRYSRGYLRH
LIKSGETLGVMLASEHNIHYMFRLIQKARNAIMNDDFTQFRKLYLSKYDEGNFNE

Sequences:

>Translated_375_residues
MFNIIKNDKNSNARIGVLELPHGKVTTPCFMPVGTLGVMKALKHDVLEKLGCNLMLANTYHLYLRPGIDVIKEYGSLHNF
TTWNKNFLTDSGGFQVFSLSNFRKIETEGVDFKSHIDGSRHYFTPESVFKMQEIFESDIIMALDICSSYGIDYSEASLYA
NITTSWARRTLRAYENRKEGYDGFLFLITQGNFFKDLRKRSTEAILELNSPGIAIGGISVGEPRDKYLEILEYNSSLIPK
DKPKYVMGIGTPHYILDAIYYGIDIFDCVNPTRIARHGSLLTDNGILRIKRAGFNVDTSPIEQDCSCTLCTRYSRGYLRH
LIKSGETLGVMLASEHNIHYMFRLIQKARNAIMNDDFTQFRKLYLSKYDEGNFNE
>Mature_375_residues
MFNIIKNDKNSNARIGVLELPHGKVTTPCFMPVGTLGVMKALKHDVLEKLGCNLMLANTYHLYLRPGIDVIKEYGSLHNF
TTWNKNFLTDSGGFQVFSLSNFRKIETEGVDFKSHIDGSRHYFTPESVFKMQEIFESDIIMALDICSSYGIDYSEASLYA
NITTSWARRTLRAYENRKEGYDGFLFLITQGNFFKDLRKRSTEAILELNSPGIAIGGISVGEPRDKYLEILEYNSSLIPK
DKPKYVMGIGTPHYILDAIYYGIDIFDCVNPTRIARHGSLLTDNGILRIKRAGFNVDTSPIEQDCSCTLCTRYSRGYLRH
LIKSGETLGVMLASEHNIHYMFRLIQKARNAIMNDDFTQFRKLYLSKYDEGNFNE

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=367, Percent_Identity=38.4196185286104, Blast_Score=276, Evalue=2e-74,
Organism=Escherichia coli, GI1786607, Length=351, Percent_Identity=40.7407407407407, Blast_Score=298, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI17544680, Length=360, Percent_Identity=36.3888888888889, Blast_Score=242, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI25146502, Length=140, Percent_Identity=30.7142857142857, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19920510, Length=367, Percent_Identity=38.6920980926431, Blast_Score=286, Evalue=1e-77,
Organism=Drosophila melanogaster, GI21355311, Length=147, Percent_Identity=31.2925170068027, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_BORHD (B2S1F3)

Other databases:

- EMBL:   CP000048
- RefSeq:   YP_001884229.1
- EnsemblBacteria:   EBBORT00000006294
- GeneID:   6276546
- GenomeReviews:   CP000048_GR
- KEGG:   bhr:BH0809
- GeneTree:   EBGT00050000007233
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- ProtClustDB:   PRK00112
- BioCyc:   BHER314723:BH0809-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 42794; Mature: 42794

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: ACT_SITE 90-90 BINDING 91-91

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNIIKNDKNSNARIGVLELPHGKVTTPCFMPVGTLGVMKALKHDVLEKLGCNLMLANTY
CCCCCCCCCCCCCEEEEEECCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHCCCEEEECEE
HLYLRPGIDVIKEYGSLHNFTTWNKNFLTDSGGFQVFSLSNFRKIETEGVDFKSHIDGSR
EEEECCCHHHHHHHCCCCCCCCCCCCEEECCCCEEEEEECCCEEECCCCCCHHHHCCCCC
HYFTPESVFKMQEIFESDIIMALDICSSYGIDYSEASLYANITTSWARRTLRAYENRKEG
EEECHHHHHHHHHHHHCCHHHEEHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCC
YDGFLFLITQGNFFKDLRKRSTEAILELNSPGIAIGGISVGEPRDKYLEILEYNSSLIPK
CCEEEEEEECCCHHHHHHHCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHHCCCCCCCC
DKPKYVMGIGTPHYILDAIYYGIDIFDCVNPTRIARHGSLLTDNGILRIKRAGFNVDTSP
CCCCEEEECCCHHHHHHHHHHCCHHHHCCCHHHHHHCCCEEECCCEEEEEECCCCCCCCC
IEQDCSCTLCTRYSRGYLRHLIKSGETLGVMLASEHNIHYMFRLIQKARNAIMNDDFTQF
CCCCCCEEHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHH
RKLYLSKYDEGNFNE
HHHHHHHCCCCCCCC
>Mature Secondary Structure
MFNIIKNDKNSNARIGVLELPHGKVTTPCFMPVGTLGVMKALKHDVLEKLGCNLMLANTY
CCCCCCCCCCCCCEEEEEECCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHCCCEEEECEE
HLYLRPGIDVIKEYGSLHNFTTWNKNFLTDSGGFQVFSLSNFRKIETEGVDFKSHIDGSR
EEEECCCHHHHHHHCCCCCCCCCCCCEEECCCCEEEEEECCCEEECCCCCCHHHHCCCCC
HYFTPESVFKMQEIFESDIIMALDICSSYGIDYSEASLYANITTSWARRTLRAYENRKEG
EEECHHHHHHHHHHHHCCHHHEEHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCC
YDGFLFLITQGNFFKDLRKRSTEAILELNSPGIAIGGISVGEPRDKYLEILEYNSSLIPK
CCEEEEEEECCCHHHHHHHCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHHCCCCCCCC
DKPKYVMGIGTPHYILDAIYYGIDIFDCVNPTRIARHGSLLTDNGILRIKRAGFNVDTSP
CCCCEEEECCCHHHHHHHHHHCCHHHHCCCHHHHHHCCCEEECCCEEEEEECCCCCCCCC
IEQDCSCTLCTRYSRGYLRHLIKSGETLGVMLASEHNIHYMFRLIQKARNAIMNDDFTQF
CCCCCCEEHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHH
RKLYLSKYDEGNFNE
HHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA