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The map label for this gene is intB [H]

Identifier: 187733993

GI number: 187733993

Start: 4485423

End: 4486691

Strand: Reverse

Name: intB [H]

Synonym: SbBS512_E4814

Alternate gene names: 187733993

Gene position: 4486691-4485423 (Counterclockwise)

Preceding gene: 187731850

Following gene: 187732744

Centisome position: 97.2

GC content: 50.91

Gene sequence:

>1269_bases
ATGGCCCTAACGGATACTAAAGTTCGCTCTGCGAAACCTGAGGAGAAAGAGTATTCACTTGTTGACGGCGACGGCATGTC
CTTACTTGTAAAACCTGGCGGTTCAAAGTATTGGCGATTTCGTTTCCGTTTTGGCGGTAAACAACATTTGATGGCGTTCG
GCGTTTACCCGGATGTTTCGCTGGCGGATGCGCGGAAGAAAAGGGAAGAGGCCAGAAAGCTGGTAGCTGCTGGTATCGAT
CCTCGTGAGCATAAGCGTGCTGTGAAAGAAGAGCAGGCGAAAGAGATTATTACTTTCGAGAAGGTTGCCAGAGAGTGGCT
CGTAACCAACCAAAAATGGTCGGAAGAGCATGCTAATCGTGTAAAAAAGAGCCTGGAGGACAATATCTTCCCGGCAATTG
GTTCCCACAATATTGCTGAACTGGGCACCCGCGATCTGTTAATTCCTATTAAAGCCGTGGAGAAATCCGGACGTCTTGAA
GTGGCTTCCCGCCTTCAACAGCGCACCACGGCCATCATGCGTTATGCAGTGCAAAGCGGGTTAATTGATTACAATCCGGC
GCAAGAGATGGCGGGGGCGGTAGCTTCCTGTAATCGACAGCATCGTCCGGCGCTGGAGTTAAAGCGTATCCCGGAATTAC
TCCAGAAAATCGATGAATACACCGGCAGGCCACTAACCCGCTGGGCAACCGAACTTACCCTGCTTATTTTTATTCGATCC
AGTGAGCTGCGTTTTGCTCGCTGGTCAGAAATCGATTTTGAAACGTCAATGTGGACCATCCCGCCTGAGCGAGAACCTAT
TCCCGGTGTGAAACATTCTCAGCGAGGAGCAAAGATGCGTACACCTCATCTGGTGCCGCTTTCAAAGCAGGCGCTAGCGA
TCCTGAAGCAGATAAAACAGTTCTGTGGCGAACATGAGCTGATTTTTATTGGCGATCACGATCCGAGTAAGCCCATGAGT
GAAAACACGGTGAACAGTGCATTGCGGGTGATGGGCTATGACACCAAGGTTGAGGTTTGCGGCCACGGCTTCCGCACTAT
GGCCTGTAGCTCATTGATTGAGTCAGGGTTATGGTCGAAGGATGCGGTGGAAAGGCAGATGAGCCATATGGAACGCAATT
CGGTGAGGGCGGCGTATATCCATAAAGCGGAACATCTGGATGAGCGCAAGCTGATGTTGCAGTGGTGGGCTGATTTTCTG
GATGCGAACCGGGAGAAGGGGATTACGCCGTTTGACTATGCGAAGATCAATCGGGGTAATGGCGAGTGA

Upstream 100 bases:

>100_bases
CTCAACCCACATCAACAGGCTTGTGAGTATACTAATGAGTATATTTGCCGATTCGATATTGCTTGTATACTCACGAGATA
CCAATGGAAGGAAGACCATT

Downstream 100 bases:

>100_bases
GTTATCTAAAAGCGCACATTCGCCTCTAAATTGAAAGCTGCTGACTTATTCAAACGAGGAATTGGTCAGCAGTGCCAAAT
AGCCGAGTTTTCACTAAGGT

Product: site-specific recombinase, phage integrase family protein

Products: NA

Alternate protein names: Int(P4) [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MALTDTKVRSAKPEEKEYSLVDGDGMSLLVKPGGSKYWRFRFRFGGKQHLMAFGVYPDVSLADARKKREEARKLVAAGID
PREHKRAVKEEQAKEIITFEKVAREWLVTNQKWSEEHANRVKKSLEDNIFPAIGSHNIAELGTRDLLIPIKAVEKSGRLE
VASRLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASCNRQHRPALELKRIPELLQKIDEYTGRPLTRWATELTLLIFIRS
SELRFARWSEIDFETSMWTIPPEREPIPGVKHSQRGAKMRTPHLVPLSKQALAILKQIKQFCGEHELIFIGDHDPSKPMS
ENTVNSALRVMGYDTKVEVCGHGFRTMACSSLIESGLWSKDAVERQMSHMERNSVRAAYIHKAEHLDERKLMLQWWADFL
DANREKGITPFDYAKINRGNGE

Sequences:

>Translated_422_residues
MALTDTKVRSAKPEEKEYSLVDGDGMSLLVKPGGSKYWRFRFRFGGKQHLMAFGVYPDVSLADARKKREEARKLVAAGID
PREHKRAVKEEQAKEIITFEKVAREWLVTNQKWSEEHANRVKKSLEDNIFPAIGSHNIAELGTRDLLIPIKAVEKSGRLE
VASRLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASCNRQHRPALELKRIPELLQKIDEYTGRPLTRWATELTLLIFIRS
SELRFARWSEIDFETSMWTIPPEREPIPGVKHSQRGAKMRTPHLVPLSKQALAILKQIKQFCGEHELIFIGDHDPSKPMS
ENTVNSALRVMGYDTKVEVCGHGFRTMACSSLIESGLWSKDAVERQMSHMERNSVRAAYIHKAEHLDERKLMLQWWADFL
DANREKGITPFDYAKINRGNGE
>Mature_421_residues
ALTDTKVRSAKPEEKEYSLVDGDGMSLLVKPGGSKYWRFRFRFGGKQHLMAFGVYPDVSLADARKKREEARKLVAAGIDP
REHKRAVKEEQAKEIITFEKVAREWLVTNQKWSEEHANRVKKSLEDNIFPAIGSHNIAELGTRDLLIPIKAVEKSGRLEV
ASRLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASCNRQHRPALELKRIPELLQKIDEYTGRPLTRWATELTLLIFIRSS
ELRFARWSEIDFETSMWTIPPEREPIPGVKHSQRGAKMRTPHLVPLSKQALAILKQIKQFCGEHELIFIGDHDPSKPMSE
NTVNSALRVMGYDTKVEVCGHGFRTMACSSLIESGLWSKDAVERQMSHMERNSVRAAYIHKAEHLDERKLMLQWWADFLD
ANREKGITPFDYAKINRGNGE

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=418, Percent_Identity=38.2775119617225, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI1788690, Length=405, Percent_Identity=33.3333333333333, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI145693166, Length=404, Percent_Identity=33.9108910891089, Blast_Score=223, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 48233; Mature: 48102

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTDTKVRSAKPEEKEYSLVDGDGMSLLVKPGGSKYWRFRFRFGGKQHLMAFGVYPDVS
CCCCCCCHHCCCCCCCCCEEECCCCCEEEEECCCCCEEEEEEEECCCEEEEEEECCCCCH
LADARKKREEARKLVAAGIDPREHKRAVKEEQAKEIITFEKVAREWLVTNQKWSEEHANR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VKKSLEDNIFPAIGSHNIAELGTRDLLIPIKAVEKSGRLEVASRLQQRTTAIMRYAVQSG
HHHHHHHCCCCCCCCCCHHHHCCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
LIDYNPAQEMAGAVASCNRQHRPALELKRIPELLQKIDEYTGRPLTRWATELTLLIFIRS
CCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEEEEC
SELRFARWSEIDFETSMWTIPPEREPIPGVKHSQRGAKMRTPHLVPLSKQALAILKQIKQ
CCCCEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
FCGEHELIFIGDHDPSKPMSENTVNSALRVMGYDTKVEVCGHGFRTMACSSLIESGLWSK
HHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEHHHCCCHHHHHHHHHHHHCCCCH
DAVERQMSHMERNSVRAAYIHKAEHLDERKLMLQWWADFLDANREKGITPFDYAKINRGN
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCHHCCCCCC
GE
CC
>Mature Secondary Structure 
ALTDTKVRSAKPEEKEYSLVDGDGMSLLVKPGGSKYWRFRFRFGGKQHLMAFGVYPDVS
CCCCCCHHCCCCCCCCCEEECCCCCEEEEECCCCCEEEEEEEECCCEEEEEEECCCCCH
LADARKKREEARKLVAAGIDPREHKRAVKEEQAKEIITFEKVAREWLVTNQKWSEEHANR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
VKKSLEDNIFPAIGSHNIAELGTRDLLIPIKAVEKSGRLEVASRLQQRTTAIMRYAVQSG
HHHHHHHCCCCCCCCCCHHHHCCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
LIDYNPAQEMAGAVASCNRQHRPALELKRIPELLQKIDEYTGRPLTRWATELTLLIFIRS
CCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEEEEC
SELRFARWSEIDFETSMWTIPPEREPIPGVKHSQRGAKMRTPHLVPLSKQALAILKQIKQ
CCCCEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
FCGEHELIFIGDHDPSKPMSENTVNSALRVMGYDTKVEVCGHGFRTMACSSLIESGLWSK
HHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEHHHCCCHHHHHHHHHHHHCCCCH
DAVERQMSHMERNSVRAAYIHKAEHLDERKLMLQWWADFLDANREKGITPFDYAKINRGN
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCHHCCCCCC
GE
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]