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The map label for this gene is kgtP

Identifier: 187733934

GI number: 187733934

Start: 2771261

End: 2772208

Strand: Reverse

Name: kgtP

Synonym: SbBS512_E2965

Alternate gene names: 187733934

Gene position: 2772208-2771261 (Counterclockwise)

Preceding gene: 187733901

Following gene: 187731672

Centisome position: 60.06

GC content: 49.05

Gene sequence:

>948_bases
ATGGCTGAAAGTACTGTAACGGCAGACAGCAAACTGACAAGTAGTGATACTCGTCGCCGCATTTGGGCGATTGTGGGGGC
CTCTTCAGGTAATCTGGTCGAGTGGTTCGATTTCTATGTCTACTCGTTCTGTTCACTCTACTTCGCCCACATTTTCTTCC
CTTCCGGGAACACGACGACTCAACTACTACAAACAGCAGGTGTTTTTGCTGCGGGATTCCTGATGCGCCCAATAGGCGGT
TGGCTATTTGGCCGCATAGCCGATAAACATGGTCGCAAAAAATCGATGCTGTTATCGGTGTGTATGATGTGTTTCGGATC
GCTGGTTATCGCCTGCCTCCCAGGTTATGAAACTATAGGTACGTGGGCTCCGGCATTATTGCTTCTCGCGCGTTTATTTC
AGGGATTATCCGTTGGCGGAGAATATGGCACCAGCGCCACCTATATGAGTGAAGTTGCCGTTGAAGGGCGCAAAGGTTTT
TACGCATCATTTCAGTATGTGACGTTGATCGGCGGACAACTGCTAGCCCTACTGGTTGTCGTGGTTTTACAACACACCAT
GGAAGACGCTGCACTCAGAGAGTGGGGATGGCGTATTCCTTTCGCGTTAGGAGCTGTGTTAGCTGTTGTGGCGTTGTGGT
TACGTCGTCAATTAGATGAAACTTCGCAACAAGAAACGCGCGCTTTAAAAGAAGCTGGATCTCTGAAAGGATTATGGCGC
AATCGCCGTGCATTCATCATGGTTCTCGGTTTTACCGCTGCGGGCTCCCTTTGTTTCTATACCTTCACCACTTATATGCA
GAAGTATCTGGTAAATACTGCGGGGATGCATGCCAACGTGGCGAGTGGCATTATGACTGCCGCATTGTTTGTATTCATGC
TTATTCAACCACTCATTGGCGCGCTGTCGGATAAGATTGGTGTAAGCGTGCAGCAAGAACCGTATTGA

Upstream 100 bases:

>100_bases
GCATAATAATACGCAACAAATGTATAACATTTTCTTTACCAAAAGAAATAAACAAAAGCGACCAACAAAAGCATCGGATT
ACGGCAGGAGACATAATGGC

Downstream 100 bases:

>100_bases
CGGGGATGTGTTATTCAGTCGGCAGTGCTACGCGCCAGGGGAGCAGTTCGCCGACCCGGTTTATCGGCCAGTCGGCTATG
ACGTCAAGGACATAGCGGAG

Product: alpha-ketoglutarate permease

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFCSLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGG
WLFGRIADKHGRKKSMLLSVCMMCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQLDETSQQETRALKEAGSLKGLWR
NRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGMHANVASGIMTAALFVFMLIQPLIGALSDKIGVSVQQEPY

Sequences:

>Translated_315_residues
MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFCSLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGG
WLFGRIADKHGRKKSMLLSVCMMCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQLDETSQQETRALKEAGSLKGLWR
NRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGMHANVASGIMTAALFVFMLIQPLIGALSDKIGVSVQQEPY
>Mature_314_residues
AESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFCSLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGW
LFGRIADKHGRKKSMLLSVCMMCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGFY
ASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQLDETSQQETRALKEAGSLKGLWRN
RRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGMHANVASGIMTAALFVFMLIQPLIGALSDKIGVSVQQEPY

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=307, Percent_Identity=100, Blast_Score=629, Evalue=0.0,
Organism=Escherichia coli, GI1790550, Length=321, Percent_Identity=33.3333333333333, Blast_Score=197, Evalue=8e-52,
Organism=Escherichia coli, GI1788292, Length=311, Percent_Identity=32.7974276527331, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1789941, Length=313, Percent_Identity=32.9073482428115, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI87082231, Length=337, Percent_Identity=24.9258160237389, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082404, Length=309, Percent_Identity=24.5954692556634, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 34617; Mature: 34486

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFCSLYFAHIFFPSGNTTT
CCCCCCCCCCCCCCCHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
QLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCMMCFGSLVIACLPGYETIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
TWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGFYASFQYVTLIGGQLLALLVV
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
VVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQLDETSQQETRALKEAGSLKGLWR
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGMHANVASGIMTAALFVFMLIQPLIG
CCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALSDKIGVSVQQEPY
HHHHHHCCCCCCCCC
>Mature Secondary Structure 
AESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFCSLYFAHIFFPSGNTTT
CCCCCCCCCCCCCCHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHH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HHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]