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The map label for this gene is ydeH [H]

Identifier: 187733341

GI number: 187733341

Start: 1553327

End: 1553713

Strand: Direct

Name: ydeH [H]

Synonym: SbBS512_E1707

Alternate gene names: 187733341

Gene position: 1553327-1553713 (Clockwise)

Preceding gene: 187733884

Following gene: 187732724

Centisome position: 33.65

GC content: 43.67

Gene sequence:

>387_bases
ATGCTGGCTATTGTTGAAAATCACTGGCAGGACGCGCATTTCGACGCCTTTCAGGAGGGGTTGCTTTCTTTTACTGCGGC
ATTAACCGATTACAAAATTTATTTGCTGACGATCCGTAGCAATATGGATGTTTTGACGGGATTGCCGGGGCGTCGGGTTC
TTGATGAATCCTTTGATCATCAGTTACGCAACACTGAGCCTCTGAATCTTTATTTAATGTTGTTGGATATTGACCGATTT
AAATTGGTTAATGATACCTACGGGCATTTAATCGGCGATGTAGTATTACGCACCCTGGCAACTTACTTAGCCAGTTGGAC
GCGTGATTACGAAACGGTTTATCGCTACGGGGGCGAAGAATTTATCATTATTGTCAAAGTGAGGTAG

Upstream 100 bases:

>100_bases
GTGGATTGATCATCTGGGGCCACTCGATAACGATGAATTACCTTACGTTCGGCTAATGGATTCTGCCCATCAACATATGC
ATAACTGTGGTCGGGAATTA

Downstream 100 bases:

>100_bases
CCTGAGTTTAACGGACACTCCTTCCTGAAATAGAATGGCATCAGAAGGAGCTAATAATGAGCAGAAAAAACCAACGTTAC
TCTAAAGAGTTCAAAGCCGA

Product: putative ggdef domain

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 128; Mature: 128

Protein sequence:

>128_residues
MLAIVENHWQDAHFDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLYLMLLDIDRF
KLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKVR

Sequences:

>Translated_128_residues
MLAIVENHWQDAHFDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLYLMLLDIDRF
KLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKVR
>Mature_128_residues
MLAIVENHWQDAHFDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLYLMLLDIDRF
KLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKVR

Specific function: A diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returnes to normal when residues 206-207 are both mutated to Ala. Overexpression also leads to a reduction in flagellar abundance and a 20-fold increase in c-di- GMP

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787816, Length=127, Percent_Identity=98.4251968503937, Blast_Score=258, Evalue=9e-71,
Organism=Escherichia coli, GI1787056, Length=123, Percent_Identity=39.8373983739837, Blast_Score=72, Evalue=1e-14,
Organism=Escherichia coli, GI1787541, Length=91, Percent_Identity=36.2637362637363, Blast_Score=70, Evalue=5e-14,
Organism=Escherichia coli, GI145693134, Length=88, Percent_Identity=42.0454545454545, Blast_Score=69, Evalue=8e-14,
Organism=Escherichia coli, GI1787802, Length=107, Percent_Identity=37.3831775700935, Blast_Score=66, Evalue=8e-13,
Organism=Escherichia coli, GI87081881, Length=95, Percent_Identity=41.0526315789474, Blast_Score=62, Evalue=8e-12,
Organism=Escherichia coli, GI87081977, Length=91, Percent_Identity=36.2637362637363, Blast_Score=61, Evalue=3e-11,
Organism=Escherichia coli, GI1788381, Length=92, Percent_Identity=39.1304347826087, Blast_Score=60, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 14947; Mature: 14947

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAIVENHWQDAHFDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDH
CEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHCCCCCHHHHHHHHH
QLRNTEPLNLYLMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEE
HHCCCCCCEEEEEEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCE
FIIIVKVR
EEEEEEEC
>Mature Secondary Structure
MLAIVENHWQDAHFDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDH
CEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHCCCCCHHHHHHHHH
QLRNTEPLNLYLMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEE
HHCCCCCCEEEEEEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCE
FIIIVKVR
EEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383113; 9097039; 9278503 [H]