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The map label for this gene is ynfL [H]

Identifier: 187733321

GI number: 187733321

Start: 1624808

End: 1625701

Strand: Reverse

Name: ynfL [H]

Synonym: SbBS512_E1781

Alternate gene names: 187733321

Gene position: 1625701-1624808 (Counterclockwise)

Preceding gene: 187732933

Following gene: 187732834

Centisome position: 35.22

GC content: 51.9

Gene sequence:

>894_bases
ATGAATATTGAACTTCGTCATCTGCGTTACTTTGTTGCTGTTGCGGAAGAGCTGCATTTCGGGCGCGCCGCTGCCCGCCT
GAATATTTCGCAACCACCGCTAAGTCAGCAGATTCAGGCACTGGAGCAACAAATTGGTGCCCGACTGCTGGCACGAACTA
ATCGCAGTGTATTGCTGACGGCAGCAGGAAAACAGTTTCTTGCAGATAGTCGGCAAATCCTGTCTATGGTAGATGACGCT
GCCGCTCGCGCTGAAAGACTGCATCAGGGTGAAGCGGGGGAGTTGCGCATTGGTTTTACTTCGTCCGCTCCTTTTATTCG
GGCGGTGTCCGATACGTTATCGCTGTTTCGCCGTGATTATCCCGGTGTCCATTTACAAACCCGCGAAATGAACACTCGTG
AGCAAATCGCTCCGCTCATTGAAGGAACGCTGGATATGGGATTGCTGCGTAACACAGCGTTACCGGAGACGCTTGAACAC
GCAGTCATCGTCCATGAACCGCTTATGGCGATGATCCCGCACGATCATCCCCTGGCAAATAACCCGAATGTAACGTTGGC
TGAACTGGCGAAAGAACCCTTCGTCTTTTTTGATCCGCACGTCGGGACAGGGCTGTATGACGATATTCTCGGGCTGATGC
GACGTTACCATTTGACGCCCGTCATCACTCAGGAGGTGGGCGAGGCAATGACCATCATCGGTCTGGTTTCCGCCGGTCTG
GGTGTTTCAATTTTGCCTGCTTCATTTAAACGTGTTCAGCTCAACGAAATGCGCTGGGTGCCGATTGCTGAAGAGGATGC
GGTTTCTGAAATGTGGTTGGTCTGGCCGAAACATCATGAACAAAGTCCAGCTTCGCGTAACTTTCGTATTCATCTGCTGA
ATGCTCTCAGGTGA

Upstream 100 bases:

>100_bases
TCCTTGCTAAATATGCCTGTGGATCAGGCTTATACATAGGTTAGGAAAATCGAATTGCTCTGTCTAATATATTAATAATC
TCAAATAAGATGTTTTAAAT

Downstream 100 bases:

>100_bases
GGGAAATTTCAGCGAAAAAGCCCGAAAAATGTGCTATTAATCACATGTCTAAGTAAAAATTTGACGACACATATTGAAGT
GCTTCACCATAGCCTACAGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDA
AARAERLHQGEAGELRIGFTSSAPFIRAVSDTLSLFRRDYPGVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVGEAMTIIGLVSAGL
GVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPASRNFRIHLLNALR

Sequences:

>Translated_297_residues
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDA
AARAERLHQGEAGELRIGFTSSAPFIRAVSDTLSLFRRDYPGVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVGEAMTIIGLVSAGL
GVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPASRNFRIHLLNALR
>Mature_297_residues
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDA
AARAERLHQGEAGELRIGFTSSAPFIRAVSDTLSLFRRDYPGVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVGEAMTIIGLVSAGL
GVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPASRNFRIHLLNALR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=297, Percent_Identity=98.989898989899, Blast_Score=597, Evalue=1e-172,
Organism=Escherichia coli, GI1788887, Length=286, Percent_Identity=36.013986013986, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI2367136, Length=257, Percent_Identity=38.1322957198444, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1790399, Length=248, Percent_Identity=31.8548387096774, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI145693105, Length=295, Percent_Identity=26.4406779661017, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788748, Length=247, Percent_Identity=28.7449392712551, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI157672245, Length=292, Percent_Identity=29.4520547945205, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI87081904, Length=195, Percent_Identity=32.8205128205128, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788297, Length=252, Percent_Identity=28.1746031746032, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790208, Length=246, Percent_Identity=26.8292682926829, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787530, Length=269, Percent_Identity=26.7657992565056, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786713, Length=258, Percent_Identity=28.2945736434109, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790262, Length=253, Percent_Identity=24.5059288537549, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787806, Length=285, Percent_Identity=23.859649122807, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787589, Length=94, Percent_Identity=43.6170212765958, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788481, Length=301, Percent_Identity=25.2491694352159, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789173, Length=130, Percent_Identity=33.0769230769231, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789506, Length=278, Percent_Identity=22.6618705035971, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=27.3037542662116, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082132, Length=299, Percent_Identity=24.7491638795987, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1787601, Length=163, Percent_Identity=32.5153374233129, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33254; Mature: 33254

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRSVLLT
CCCCHHHHHHHHHHHHHHHHCHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
AAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSSAPFIRAVSDTLSLFRRDY
ECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCC
PGVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEHAVIVHEPLMAMIPHDHPLAN
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHCCCCCCCCC
NPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVGEAMTIIGLVSAGL
CCCEEHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
GVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPASRNFRIHLLNALR
CCEECCCCHHHEECCCEEECCCCCHHHHHHHEEECCCCCCCCCCCCCCEEEEEECCC
>Mature Secondary Structure
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRSVLLT
CCCCHHHHHHHHHHHHHHHHCHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
AAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSSAPFIRAVSDTLSLFRRDY
ECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCC
PGVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEHAVIVHEPLMAMIPHDHPLAN
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHCCCCCCCCC
NPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVGEAMTIIGLVSAGL
CCCEEHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
GVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPASRNFRIHLLNALR
CCEECCCCHHHEECCCEEECCCCCHHHHHHHEEECCCCCCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]