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The map label for this gene is menF

Identifier: 187733268

GI number: 187733268

Start: 2444440

End: 2445735

Strand: Reverse

Name: menF

Synonym: SbBS512_E2644

Alternate gene names: 187733268

Gene position: 2445735-2444440 (Counterclockwise)

Preceding gene: 187733797

Following gene: 187730990

Centisome position: 52.98

GC content: 53.47

Gene sequence:

>1296_bases
GTGCAATCACTTACTACGGCGCTGGAAAATCTACTGCGCCATTTGTCGCAAGAGATTCCGGCGACACCCGGCATTCGGGT
TATCGATATTCCTTTCCCTCTCAAAGACGCTTTTGATGCCTTGAGCTGGCTGGCCAGTCAGCAAACGTACCCGCAATTCT
ACTGGCAACAACGTAATGGTGATGAAGAAGCTGCCGTCCTGGGCGCGATTACCCGTTTTACGTCGTTGGACCAGGCACAA
CGTTTTCTTCGCCAGCACCCGGAACACGCCGACTTACGCATTTGGGGGCTGAATGCTTTTGACCCGTCGCAGGGCAATTT
ACTTTTACCCCGCTTGGAATGGCGACGCTGTGGCGGTAAAGCCACGCTGCGGCTGACGCTATTCAGCGAAAGCTCCCTTC
AGCACGATGCGATTCAGGCAAAAGAATTTATCGCCACACTGGTGAGTATCAAGCCCTTGCCTGGGTTACATTTAACCACC
ACGCGAGAACAACACTGGCCGGACAAAACGGGCTGGACGCAATTAATCGAACTGGCAACGAAAACCATCGCCGAAGGTGA
GCTCGACAAAGTGGTGCTCGCTCGGGCAACTGACCTGCATTTCGCAAGTCCGGTCAACGCGGCGGCGATGATGGCTGCCA
GTCGTCGACTGAATCTGAATTGCTACCATTTTTACATGGCCTTTGATGGCGAAAATGCTTTTCTTGGCTCTTCACCGGAA
CGGTTATGGCGGCGGCGTGACAAAGCGCTGCGTACTGAAGCGCTGGCGGGAACAGTAGCAAATCATCCTGATGATAAGCA
GGCGCAGCAGTTAGAAGAGTGGCTGATGGCGGATGATAAAAACCAGCGCGAGAACATGCTGGTGGTGGAAGATATCTGCC
AACGATTACAGGCCGATACCCAGACGCTGGATGTTTTACCGCCGCAGGTACTGCGTCTGCGTAAAGTGCAGCATCTTCGC
CGCTGTATCTGGACTTCACTCAACAAAGCGGATGATGTGATCTGTTTACATCAGTTGCAGCCAACGGCAGCAGTTGCTGG
CTTACCGCGCGATCTGGCGCGACAGTTTATCGCCCGTCACGAACCGTTCACCCGAGAATGGTACGCCGGTTCTGCGGGCT
ATCTCTCATTACAACAAAGCGAATTCTGCGTTTCCCTGCGCTCAGCAAAAATTAGCGGCAATGTCGTGCGATTATATGCT
GGCGCGGGCATTGTCCGTGGTTCCGACCCCGAGCAAGAGTGGCAGGAAATCGACAACAAAGCGGCAGGGTTGCGTACTTT
ATTACAAATGGAATAG

Upstream 100 bases:

>100_bases
ACTGTTGCTGGCACGCCGTTAAAACCTCCCTACACCGGGGTACTGCATTTTTTTTGTAGTACCCCGTATAATGTGAGGCT
TTTAACAGGGAGAGGTCCGC

Downstream 100 bases:

>100_bases
TAATGAGTCGCATCATTACCGATTCATATCAATAATCTATTTTTGTAGCTCCTTATACTTAGCCGCAATATTGATACCGG
ACAAACTCATGTCAGTAAGC

Product: isochorismate synthase, menaquinone-specific

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWLASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQ
RFLRQHPEHADLRIWGLNAFDPSQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLNLNCYHFYMAFDGENAFLGSSPE
RLWRRRDKALRTEALAGTVANHPDDKQAQQLEEWLMADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLR
RCIWTSLNKADDVICLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKISGNVVRLYA
GAGIVRGSDPEQEWQEIDNKAAGLRTLLQME

Sequences:

>Translated_431_residues
MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWLASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQ
RFLRQHPEHADLRIWGLNAFDPSQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLNLNCYHFYMAFDGENAFLGSSPE
RLWRRRDKALRTEALAGTVANHPDDKQAQQLEEWLMADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLR
RCIWTSLNKADDVICLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKISGNVVRLYA
GAGIVRGSDPEQEWQEIDNKAAGLRTLLQME
>Mature_431_residues
MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWLASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQ
RFLRQHPEHADLRIWGLNAFDPSQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLNLNCYHFYMAFDGENAFLGSSPE
RLWRRRDKALRTEALAGTVANHPDDKQAQQLEEWLMADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLR
RCIWTSLNKADDVICLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKISGNVVRLYA
GAGIVRGSDPEQEWQEIDNKAAGLRTLLQME

Specific function: Menaquinone biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI87082077, Length=431, Percent_Identity=99.3039443155453, Blast_Score=880, Evalue=0.0,
Organism=Escherichia coli, GI1786809, Length=276, Percent_Identity=31.1594202898551, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI1787518, Length=295, Percent_Identity=26.7796610169492, Blast_Score=73, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320935, Length=327, Percent_Identity=27.5229357798165, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 48832; Mature: 48832

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWLASQQTYPQFYWQQRNG
CCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHCCCC
DEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDPSQGNLLLPRLEWRRCGGK
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEECCCHHHHHCCCC
ATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTTTREQHWPDKTGWTQLIELAT
EEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHCCCCCCHHHHHHHHHH
KTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLNLNCYHFYMAFDGENAFLGSSPE
HHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCHH
RLWRRRDKALRTEALAGTVANHPDDKQAQQLEEWLMADDKNQRENMLVVEDICQRLQADT
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHCCC
QTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVICLHQLQPTAAVAGLPRDLARQFIARH
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHC
EPFTREWYAGSAGYLSLQQSEFCVSLRSAKISGNVVRLYAGAGIVRGSDPEQEWQEIDNK
CCCCHHHCCCCCCEEEECHHHHHHHHHHHCCCCCEEEEEECCCEECCCCCHHHHHHHHHH
AAGLRTLLQME
HHHHHHHHHCC
>Mature Secondary Structure
MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWLASQQTYPQFYWQQRNG
CCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHCCCC
DEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDPSQGNLLLPRLEWRRCGGK
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEECCCHHHHHCCCC
ATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTTTREQHWPDKTGWTQLIELAT
EEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHCCCCCCHHHHHHHHHH
KTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLNLNCYHFYMAFDGENAFLGSSPE
HHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCHH
RLWRRRDKALRTEALAGTVANHPDDKQAQQLEEWLMADDKNQRENMLVVEDICQRLQADT
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHCCC
QTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVICLHQLQPTAAVAGLPRDLARQFIARH
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHC
EPFTREWYAGSAGYLSLQQSEFCVSLRSAKISGNVVRLYAGAGIVRGSDPEQEWQEIDNK
CCCCHHHCCCCCCEEEECHHHHHHHHHHHCCCCCEEEEEECCCEECCCCCHHHHHHHHHH
AAGLRTLLQME
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8549818; 8764478; 9205837; 9278503; 2666397; 7984428 [H]