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The map label for this gene is insK [C]

Identifier: 187733190

GI number: 187733190

Start: 1517446

End: 1518336

Strand: Reverse

Name: insK [C]

Synonym: SbBS512_E1664

Alternate gene names: 187733190

Gene position: 1518336-1517446 (Counterclockwise)

Preceding gene: 187730293

Following gene: 187732368

Centisome position: 32.89

GC content: 53.98

Gene sequence:

>891_bases
ATGATGCCACTGCTGGATAAGCTGCGTGAGCAGTACGGGGTCGGACCGCTATGCAGCGAACTGCATATTGCCCCGTCAAC
GTATTACCACTGTCAGCAACAGCGACATCATCCGGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAG
AGATACAGCGCGTATACGATGAAAATCACAAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGAAAGGCATC
AGAGTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTAC
GACCATCAGCCGGAAAGCCGTTGCCGCAGGCGACCGCGTAAACCGTCAGTTCGTGGCAGAACGACCTGACCAGCTGTGGG
TGGCTGATTTTACTTACGTCAGCACATGGCAGGGCTTCGTCTATGTGGCGTTCATCATTGATGTGTTTGCCGGATACATC
GTGGGGTGGCGGGTCTCATCGTCCATGGAAACGACATTCGTGCTGGATGCTCTGGAGCAGGCGTTATGGGCCCGTCGACC
GTCCGGCACGGTCCATCACAGTGATAAAGGTTCTCAGTATGTATCGCTGGCCTACACACAGCGGCTTAAGGAAGCCGGAT
TACTGGCATCAACAGGAAGTACAGGCGACTCGTATGACAACGCGATGGCGGAGAGCATCAATGGTCTTTACAAAGCGGAG
GTAATACACCGTAAGAGCTGGAAAAACCGTGCAGAAGTGGAACTGGCCACACTCACGTGGGTGGACTGGTATAATAATCG
ACGATTGCTGGAAAGGCTGGGCCACATCCCTCCGGCAGAAGCAGAAAAAGCTTATTATGCTTCTATCGGAAACGATGATC
TGGCAGCCTGA

Upstream 100 bases:

>100_bases
AGCTGGAGCGTGAAAATCGTGAACTGCGCCGCAGTAACGATATCCTTCGCCAGGCTTCCGCTTATTTTGCGAAGGCGGAG
TTCGACCGCCTCTGGAAAAA

Downstream 100 bases:

>100_bases
GTTCACAGATAAAACATTCTCCAGGAAACTTGGGGCGGTTCACGCCTCATTTCACACTCCTGTTGACCACCTGGTCTTAA
CTGGAATTTAAATGCCTGTA

Product: IS1203 transposase orfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MMPLLDKLREQYGVGPLCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEIQRVYDENHKVYGVRKVWRQLLRKGI
RVARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYI
VGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAE
VIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNDDLAA

Sequences:

>Translated_296_residues
MMPLLDKLREQYGVGPLCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEIQRVYDENHKVYGVRKVWRQLLRKGI
RVARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYI
VGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAE
VIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNDDLAA
>Mature_296_residues
MMPLLDKLREQYGVGPLCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEIQRVYDENHKVYGVRKVWRQLLRKGI
RVARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYI
VGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAE
VIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNDDLAA

Specific function: Involved in the transposition of the insertion sequence [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=260, Percent_Identity=50, Blast_Score=251, Evalue=5e-67,
Organism=Escherichia coli, GI1789981, Length=286, Percent_Identity=29.7202797202797, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1788405, Length=292, Percent_Identity=27.3972602739726, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787263, Length=292, Percent_Identity=27.3972602739726, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786753, Length=292, Percent_Identity=27.3972602739726, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786570, Length=292, Percent_Identity=27.3972602739726, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786490, Length=292, Percent_Identity=27.3972602739726, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 33792; Mature: 33792

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPLLDKLREQYGVGPLCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEIQRVYD
CCHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ENHKVYGVRKVWRQLLRKGIRVARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRV
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHH
NRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQ
HHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHH
ALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAE
HHHHCCCCCCCEECCCCCCEEHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHH
VIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNDDLAA
HHHHHHCCCCCCEEEEEEEEEHHHCCHHHHHHHCCCCCCCCCHHHEEECCCCCCCC
>Mature Secondary Structure
MMPLLDKLREQYGVGPLCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEIQRVYD
CCHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ENHKVYGVRKVWRQLLRKGIRVARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRV
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHH
NRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQ
HHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHH
ALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAE
HHHHCCCCCCCEECCCCCCEEHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHH
VIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNDDLAA
HHHHHHCCCCCCEEEEEEEEEHHHCCHHHHHHHCCCCCCCCCHHHEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2159625; 2824781 [H]