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The map label for this gene is nemA

Identifier: 187732900

GI number: 187732900

Start: 1683970

End: 1685067

Strand: Direct

Name: nemA

Synonym: SbBS512_E1842

Alternate gene names: 187732900

Gene position: 1683970-1685067 (Clockwise)

Preceding gene: 187732442

Following gene: 187731273

Centisome position: 36.48

GC content: 53.1

Gene sequence:

>1098_bases
ATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGAC
GCGTCTGCGCAGTATTGAACCGGGTGATATTCCTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTT
TGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCCTGGCATCCATAGTCCGGAGCAA
ATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACG
CATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTC
TGCGCGATGAAAATAGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTAGAAGAGATTCCAGGTATC
GTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGCTCCTCTCTGCTCACGGTTA
TTTGCTGCATCAGTTCCTTTCTCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTC
TGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCATTTCGCCAATCGGTACT
TTCCAGAACACGGATAACGGCCCGAATGAAGAAGCCGATACACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGC
TTATCTGCATATGTCAGAACCAGATTGGTCGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTT
TCCACGGTCCGATTATCTGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCG
GTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCG
TGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAA

Upstream 100 bases:

>100_bases
TTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCA
CCAGACGACCGGGAGCCTTT

Downstream 100 bases:

>100_bases
TCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAG
ATGCGTCTTCTTCATACCAT

Product: N-ethylmaleimide reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQ
IAAWKKITAGVHAENGHMAVQLWHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENSQAIRVETSMPRALELEEIPGI
VNDFRQAIANAREAGFDLVELLSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIEEWGADRIGIRISPIGT
FQNTDNGPNEEADTLYLIEQLGKRGIAYLHMSEPDWSGGEPYTDAFREKVRARFHGPIICAGAYTVEKAETLIGKGLIDA
VAFGRDWIANPDLVARLQRKAELNPQRAESFYGGGAEGYTDYPTL

Sequences:

>Translated_365_residues
MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQ
IAAWKKITAGVHAENGHMAVQLWHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENSQAIRVETSMPRALELEEIPGI
VNDFRQAIANAREAGFDLVELLSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIEEWGADRIGIRISPIGT
FQNTDNGPNEEADTLYLIEQLGKRGIAYLHMSEPDWSGGEPYTDAFREKVRARFHGPIICAGAYTVEKAETLIGKGLIDA
VAFGRDWIANPDLVARLQRKAELNPQRAESFYGGGAEGYTDYPTL
>Mature_364_residues
SSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQI
AAWKKITAGVHAENGHMAVQLWHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENSQAIRVETSMPRALELEEIPGIV
NDFRQAIANAREAGFDLVELLSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIEEWGADRIGIRISPIGTF
QNTDNGPNEEADTLYLIEQLGKRGIAYLHMSEPDWSGGEPYTDAFREKVRARFHGPIICAGAYTVEKAETLIGKGLIDAV
AFGRDWIANPDLVARLQRKAELNPQRAESFYGGGAEGYTDYPTL

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=365, Percent_Identity=98.3561643835616, Blast_Score=732, Evalue=0.0,
Organism=Escherichia coli, GI1789463, Length=359, Percent_Identity=31.1977715877437, Blast_Score=121, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17565138, Length=356, Percent_Identity=30.0561797752809, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17559802, Length=352, Percent_Identity=28.6931818181818, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17564188, Length=351, Percent_Identity=26.4957264957265, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17559804, Length=268, Percent_Identity=29.1044776119403, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI72001454, Length=354, Percent_Identity=29.6610169491525, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17540738, Length=374, Percent_Identity=27.5401069518717, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6325086, Length=389, Percent_Identity=38.8174807197943, Blast_Score=233, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6321973, Length=389, Percent_Identity=37.0179948586118, Blast_Score=218, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39629; Mature: 39498

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEAT
CCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEHHH
QISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQLWHTGRISHASLQPGGQAP
HHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEECCCCEEEEEEECCCCCCCCCCCCCCCC
VAPSALSAGTRTSLRDENSQAIRVETSMPRALELEEIPGIVNDFRQAIANAREAGFDLVE
CCCHHHCCCCCCCCCCCCCCEEEEECCCCCEECHHHCCCHHHHHHHHHHHHHHCCHHHHH
LLSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIEEWGADRIGIRISPIGT
HHHHHHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
FQNTDNGPNEEADTLYLIEQLGKRGIAYLHMSEPDWSGGEPYTDAFREKVRARFHGPIIC
CCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEE
AGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLVARLQRKAELNPQRAESFYGGGAEGYT
ECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCC
DYPTL
CCCCC
>Mature Secondary Structure 
SSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEAT
CCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEHHH
QISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQLWHTGRISHASLQPGGQAP
HHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEECCCCEEEEEEECCCCCCCCCCCCCCCC
VAPSALSAGTRTSLRDENSQAIRVETSMPRALELEEIPGIVNDFRQAIANAREAGFDLVE
CCCHHHCCCCCCCCCCCCCCEEEEECCCCCEECHHHCCCHHHHHHHHHHHHHHCCHHHHH
LLSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIEEWGADRIGIRISPIGT
HHHHHHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
FQNTDNGPNEEADTLYLIEQLGKRGIAYLHMSEPDWSGGEPYTDAFREKVRARFHGPIIC
CCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEE
AGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLVARLQRKAELNPQRAESFYGGGAEGYT
ECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCC
DYPTL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]