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The map label for this gene is phnE

Identifier: 187732726

GI number: 187732726

Start: 4298317

End: 4299096

Strand: Reverse

Name: phnE

Synonym: SbBS512_E4605

Alternate gene names: 187732726

Gene position: 4299096-4298317 (Counterclockwise)

Preceding gene: 187731575

Following gene: 187733940

Centisome position: 93.13

GC content: 60.51

Gene sequence:

>780_bases
ATGCAAACCATCACCATCGCCCCACCCAAGCGTAGCTGGTTCTCGCTTCTGAGCTGTGCCGTTGTTCTCGCCGTGCTGGT
CGTCTCGTGGCAGGGCGCGGAAATGGCCCCGCTTACGCTGATTAAAGACGGCGGCAACATGGCAACCTTCGTTGCCGACT
TCTTCCCGCCCGATTTCAGCCAGTGGCAGGATTACCTCACCGAAATGGCCGTCACGCTGCAAATCGCCGTCTGGGGCACC
GCGCTGGCAGTGGTTCTCTCCATCCCCTTCGGTCTGATGAGCGCCGAAAACCTGGTGCCGTGGTGGGTTTACCAGCCCGT
TCGCCGCCTGATGGATGCCTGCCGCGCCATTAACGAAATGGTCTTCGCCATGCTGTTCGTGGTCGCCGTCGGTCTCGGCC
CGTTCGCTGGCGTGCTGGCGCTATTTATCCACACCACCGGCGTGCTCTCCAAGCTGCTTTCCGAAGCGGTGGAAGCGATC
GAACCCGGCCCGGTAGAAGGCATTCGCGCCACCGGCGCCAACAAGCTGGAAGAGATCCTTTACGGCGTGCTGCCACAGGT
AATGCCGCTGCTGATCTCCTACTCCCTCTATCGCTTCGAATCCAACGTCCGCTCGGCGACCGTCGTCGGCATGGTCGGTG
CAGGCGGGATCGGCGTCACCCTGTGGGAAGCGATTCGCGGTTTTCAGTTCCAACAAACCTGCGCCCTGATGGTGCTTATC
ATCGTCACGGTCAGCCTGCTGGATTTCCTCTCTCAACGGTTGCGTAAGCACTTTATCTGA

Upstream 100 bases:

>100_bases
TGAGGGAGAGGGTTAGGGTGAGGGGGAAATGCGCACGCTGATGCCCTCATCCCGGCCCTCTCCCACAGGGAGAGGGAGAA
AACATTAAACGGAGCCAAAC

Downstream 100 bases:

>100_bases
TAAGCGAGGCATTGATATCTATGCACTTGTCTACACATCCGACCAGCTACCCAACACGCTATCAGGAGATAGCCGCAAAA
CTTGAGCAGGAGCTTCGTCA

Product: phosphonate ABC transporter permease

Products: 3-phospho-serine [Cytoplasm]; phosphite [Cytoplasm]; ADP; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MQTITIAPPKRSWFSLLSCAVVLAVLVVSWQGAEMAPLTLIKDGGNMATFVADFFPPDFSQWQDYLTEMAVTLQIAVWGT
ALAVVLSIPFGLMSAENLVPWWVYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIGVTLWEAIRGFQFQQTCALMVLI
IVTVSLLDFLSQRLRKHFI

Sequences:

>Translated_259_residues
MQTITIAPPKRSWFSLLSCAVVLAVLVVSWQGAEMAPLTLIKDGGNMATFVADFFPPDFSQWQDYLTEMAVTLQIAVWGT
ALAVVLSIPFGLMSAENLVPWWVYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIGVTLWEAIRGFQFQQTCALMVLI
IVTVSLLDFLSQRLRKHFI
>Mature_259_residues
MQTITIAPPKRSWFSLLSCAVVLAVLVVSWQGAEMAPLTLIKDGGNMATFVADFFPPDFSQWQDYLTEMAVTLQIAVWGT
ALAVVLSIPFGLMSAENLVPWWVYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIGVTLWEAIRGFQFQQTCALMVLI
IVTVSLLDFLSQRLRKHFI

Specific function: Part of the binding-protein-dependent transport system for phosphonates; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG3639

COG function: function code P; ABC-type phosphate/phosphonate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005769 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 28329; Mature: 28329

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTITIAPPKRSWFSLLSCAVVLAVLVVSWQGAEMAPLTLIKDGGNMATFVADFFPPDFS
CCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHCCCCHH
QWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWWVYQPVRRLMDACRAINEM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
VFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAIEPGPVEGIRATGANKLEEIL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHH
YGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIGVTLWEAIRGFQFQQTCALMVLI
HHHHHHHHHHHHHHHHHHHHHCCCHHHHEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHH
IVTVSLLDFLSQRLRKHFI
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQTITIAPPKRSWFSLLSCAVVLAVLVVSWQGAEMAPLTLIKDGGNMATFVADFFPPDFS
CCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHCCCCHH
QWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWWVYQPVRRLMDACRAINEM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
VFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAIEPGPVEGIRATGANKLEEIL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHH
YGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIGVTLWEAIRGFQFQQTCALMVLI
HHHHHHHHHHHHHHHHHHHHHCCCHHHHEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHH
IVTVSLLDFLSQRLRKHFI
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]