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The map label for this gene is insO2 [H]

Identifier: 187732573

GI number: 187732573

Start: 1763997

End: 1764518

Strand: Direct

Name: insO2 [H]

Synonym: SbBS512_E1925

Alternate gene names: 187732573

Gene position: 1763997-1764518 (Clockwise)

Preceding gene: 187732945

Following gene: 187732938

Centisome position: 38.21

GC content: 54.21

Gene sequence:

>522_bases
GTGACCGAGCCAAATCAGGTGTGGTGCGGTGATGTGACCTGTATCTGGACGGGTAAGCGCTGGGCGTACCTCGCCGTTGT
TCTCGACCTGTTCGCAAGAAAACCAGTGGGCTGGGCCATGTCGTTCTCGCCGGACAGCAGGCTCACCATGAAAGCGCTGG
AAATGGCATGGGAAACCCGTGGTAAGCCCGGCGGGGTGATGTTCCACAGCGATCAGGGCAGTCATTATACGAGCAGGCAG
TTCCGGCAGTTATTGTGGCGATACCAGATCAGACAGAGTATGAGCCGGCGCGGAAACTGCTGGGATAACAGCCCAATGGA
ACGCTTCTTCAGGAGTCTGAAGAACGAATGGATGCCGGTGGTGGGTTACGTAAGCTTCAGCGAGGCAGCTCACGCCATAA
CGGACTATATCGTTGGATATTACAGCGCACTAAGACCGCACGAATATAACGGTGGGTTACCCCCAAACGAATCGGAAAAT
CGATACTGGAAAAACTCTAACTCGGTGGCCAGTTTTTGTTGA

Upstream 100 bases:

>100_bases
AAAAAGCTTATTATGCTTCCATCGGAAACGATGATCTGGCAGCCTGAGTTCACAGATAAAACACTCTCCAGGAAACCCGG
GGCGGTTCAGGCAGTTCGCC

Downstream 100 bases:

>100_bases
CCACTTCAAACCGCAAAAAAGCGAACGTCATGCGCCCGGTGGAGTACCAGAACGGCGAAGCATTCACTATCCGTAACGTA
CGGGTTATGCCTGAATCCAT

Product: IS911, transposase orfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MTEPNQVWCGDVTCIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQ
FRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESEN
RYWKNSNSVASFC

Sequences:

>Translated_173_residues
MTEPNQVWCGDVTCIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQ
FRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESEN
RYWKNSNSVASFC
>Mature_172_residues
TEPNQVWCGDVTCIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQF
RQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENR
YWKNSNSVASFC

Specific function: Involved in the transposition of the insertion sequence IS911B [H]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=132, Percent_Identity=35.6060606060606, Blast_Score=85, Evalue=4e-17,
Organism=Escherichia coli, GI1788405, Length=158, Percent_Identity=39.873417721519, Blast_Score=121, Evalue=3e-29,
Organism=Escherichia coli, GI1787263, Length=158, Percent_Identity=39.873417721519, Blast_Score=121, Evalue=3e-29,
Organism=Escherichia coli, GI1786753, Length=158, Percent_Identity=39.873417721519, Blast_Score=121, Evalue=3e-29,
Organism=Escherichia coli, GI1786570, Length=158, Percent_Identity=39.873417721519, Blast_Score=121, Evalue=3e-29,
Organism=Escherichia coli, GI1786490, Length=158, Percent_Identity=39.873417721519, Blast_Score=121, Evalue=3e-29,
Organism=Escherichia coli, GI1789981, Length=162, Percent_Identity=35.1851851851852, Blast_Score=102, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 20158; Mature: 20027

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPNQVWCGDVTCIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETR
CCCCCCEEECCEEEEEECCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHC
GKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPV
CCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCE
VGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVASFC
EEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCCC
>Mature Secondary Structure 
TEPNQVWCGDVTCIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETR
CCCCCEEECCEEEEEECCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHC
GKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPV
CCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCE
VGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVASFC
EEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]