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The map label for this gene is xylF

Identifier: 187732099

GI number: 187732099

Start: 3691545

End: 3692552

Strand: Reverse

Name: xylF

Synonym: SbBS512_E3956

Alternate gene names: 187732099

Gene position: 3692552-3691545 (Counterclockwise)

Preceding gene: 187732380

Following gene: 187730122

Centisome position: 79.99

GC content: 44.44

Gene sequence:

>1008_bases
ATGCCATTCTATTTCAGGAAGGAGTGTCCGTTAAACTCAGGCTACCTCAACATTCTACTCACCCTTTGCACCTCACTTCT
GCTTACCAACGTTGCGGCACACGCCAAAGAAGTCAAAATAGGTATGGCGATTGATGATCTACGTCTTGAACGCTGGCAAA
AAGATCGAGATATTTTTGTGAAAAAGGCTGAATCTCTCGGTGCGAAAGTATTTGTACAGTCTGCAAATGGCAATGGAGAA
ACACAAATGTCGCAGATTGAAAACATGATTAACCGGGGTGTCGATGTTCTTGTCATTATTCCGTATAACGGCCAGGTATT
AAGTAACGTTGTAAAAGAAGCCAAACAAGAAGGTATTAAAGTATTAGCTTACGACCGTATGATTAACGATGCGGATATCG
ATTTTTATATTTCTTTCGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAAT
TACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTA
CGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCGCTAACCGCCAATA
ATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTA
TCAGGGAAAGTAGCAATTTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGAC
GGTGTATAAACCTATTACATTGCTGGCAAATACTGCCGCAGAAATTGCCGTTGAATTGGGCAATGGTCAGGAGCCAAAAG
CAGATACCTCACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATC
AAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAA

Upstream 100 bases:

>100_bases
CACTGATCGAAAGTTGATTTTCAAGAACCGTTCTGACAGCTTCGGCTTTGAACTCTTTAGAGTAACGTTGGTTTTTTCTG
CTCATTATTAGCTCCTTCTG

Downstream 100 bases:

>100_bases
GCGTTACGCCCCGGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATG
CCTTATCTACTTGAAATGAA

Product: D-xylose transporter subunit XylF

Products: ADP; phosphate; xylose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MPFYFRKECPLNSGYLNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDRDIFVKKAESLGAKVFVQSANGNGE
TQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEAKQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGN
YFLMGGSPVDNNAKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALTANNNKIDAVVASNDATAGGAIQALSAQGL
SGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAEIAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNI
KDTVIKDGFHKESEL

Sequences:

>Translated_335_residues
MPFYFRKECPLNSGYLNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDRDIFVKKAESLGAKVFVQSANGNGE
TQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEAKQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGN
YFLMGGSPVDNNAKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALTANNNKIDAVVASNDATAGGAIQALSAQGL
SGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAEIAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNI
KDTVIKDGFHKESEL
>Mature_334_residues
PFYFRKECPLNSGYLNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDRDIFVKKAESLGAKVFVQSANGNGET
QMSQIENMINRGVDVLVIIPYNGQVLSNVVKEAKQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNY
FLMGGSPVDNNAKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALTANNNKIDAVVASNDATAGGAIQALSAQGLS
GKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAEIAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIK
DTVIKDGFHKESEL

Specific function: Involved in the high-affinity D-xylose membrane transport system. Binds with high affinity to xylose [H]

COG id: COG4213

COG function: function code G; ABC-type xylose transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1789990, Length=326, Percent_Identity=97.239263803681, Blast_Score=605, Evalue=1e-174,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761
- InterPro:   IPR013456 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36295; Mature: 36164

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFYFRKECPLNSGYLNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDRDIFV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHCCCHHHHH
KKAESLGAKVFVQSANGNGETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEAKQEGIK
HHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCE
VLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNNAKLFRAGQ
EEEEECCCCCCCEEEEEEECCCCHHHHHHEEHEEEECCCCEEEECCCCCCCCCEEEECCC
MKVLKPYVDSGKIKVVGDQWVDGWLPENALTANNNKIDAVVASNDATAGGAIQALSAQGL
EEEEEECCCCCCEEEEECCCCCCCCCCCCEECCCCEEEEEEECCCCCCCCCEEEECCCCC
SGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAEIAVELGNGQEPKADTSLNN
CCEEEECCCCCCHHHHHHHHCCCCEEEEECCHHHHHCCHHHEEEECCCCCCCCCCCCHHC
GLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL
CHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PFYFRKECPLNSGYLNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDRDIFV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHCCCHHHHH
KKAESLGAKVFVQSANGNGETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEAKQEGIK
HHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCE
VLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNNAKLFRAGQ
EEEEECCCCCCCEEEEEEECCCCHHHHHHEEHEEEECCCCEEEECCCCCCCCCEEEECCC
MKVLKPYVDSGKIKVVGDQWVDGWLPENALTANNNKIDAVVASNDATAGGAIQALSAQGL
EEEEEECCCCCCEEEEECCCCCCCCCCCCEECCCCEEEEEEECCCCCCCCCEEEECCCCC
SGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAEIAVELGNGQEPKADTSLNN
CCEEEECCCCCCHHHHHHHHCCCCEEEEECCHHHHHCCHHHEEEECCCCCCCCCCCCHHC
GLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL
CHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xylose [Periplasm]; H2O [C]

Specific reaction: ATP + xylose [Periplasm] + H2O = ADP + phosphate + xylose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 8581399; 9298646 [H]