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The map label for this gene is insB1 [H]

Identifier: 187732084

GI number: 187732084

Start: 1478498

End: 1479160

Strand: Reverse

Name: insB1 [H]

Synonym: SbBS512_E1621

Alternate gene names: 187732084

Gene position: 1479160-1478498 (Counterclockwise)

Preceding gene: 187730368

Following gene: 187730938

Centisome position: 32.04

GC content: 51.58

Gene sequence:

>663_bases
ATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAACACGGTTTTACGTCACTTAAAAAACTCAGGCCACAGTCGGT
AACCTCGCGCATACAACCGGGCAGTGATGTGATTGTCTGCGCTGAAATGGACGAACAGTGGGGCTACGTCGGTGCTAAAT
CACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTG
GCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATGGATGACGGATGGCTGGCCGCTGTATGA
ATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGAC
AACATCTGGCAAGGCTGGGACGGAAGTCACTGTCGTTCTCAAAATCGGTGGAGCTGCATGACAAGGTCATCGGGCATTAT
CTGACTATCAGTAAGTTGGAGTCATTACCAATTTTCGTACCCATTTACCGTTTATCCCTTGTACATATTCCCCCGGTTTG
GCCCGAGCTACGAGCTTTTGCCCGGCGAGTTTCGCGATATCATCTACAGACACATTATTTTGCTTTGCCAGTTGTTGGTA
ACTCGCTTTACGTTCGGCATTAA

Upstream 100 bases:

>100_bases
GCTCTCATTGCCGTAAAACATGGCAACTACAGTTCACTTACACCGCCTCTCAGCCCGGTACGCACCAGAAAATCATTGAT
ATGGCCATGAATGGCGTCGG

Downstream 100 bases:

>100_bases
TGTCGGTTACTAATTTCTCTGTTTCAGCATCCGTTTTCAGCGCAACCAGATAACCATAAAATGTTTCCCCGACCCGCCCC
TGCGTTCTGGCTTCATCCAG

Product: IS1 transposase orfB

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MSRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTL
ATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHY
LTISKLESLPIFVPIYRLSLVHIPPVWPELRAFARRVSRYHLQTHYFALPVVGNSLYVRH

Sequences:

>Translated_220_residues
MSRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTL
ATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHY
LTISKLESLPIFVPIYRLSLVHIPPVWPELRAFARRVSRYHLQTHYFALPVVGNSLYVRH
>Mature_219_residues
SRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLA
TLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYL
TISKLESLPIFVPIYRLSLVHIPPVWPELRAFARRVSRYHLQTHYFALPVVGNSLYVRH

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=163, Percent_Identity=90.1840490797546, Blast_Score=307, Evalue=4e-85,
Organism=Escherichia coli, GI1788203, Length=163, Percent_Identity=90.1840490797546, Blast_Score=307, Evalue=4e-85,
Organism=Escherichia coli, GI1786203, Length=163, Percent_Identity=90.1840490797546, Blast_Score=307, Evalue=4e-85,
Organism=Escherichia coli, GI1787223, Length=163, Percent_Identity=88.3435582822086, Blast_Score=301, Evalue=3e-83,
Organism=Escherichia coli, GI1786470, Length=157, Percent_Identity=91.0828025477707, Blast_Score=300, Evalue=7e-83,
Organism=Escherichia coli, GI1786459, Length=157, Percent_Identity=91.0828025477707, Blast_Score=300, Evalue=7e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 25891; Mature: 25759

Theoretical pI: Translated: 10.94; Mature: 10.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLF
CCCCCCCCCCCCCHHHHHHHHCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCHHH
YAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHCCHHHHHHHH
YTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLTISKLESLPIFVPIYRLSL
HHHHHHHCCCCHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHEEE
VHIPPVWPELRAFARRVSRYHLQTHYFALPVVGNSLYVRH
EECCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCEEEEEC
>Mature Secondary Structure 
SRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLF
CCCCCCCCCCCCHHHHHHHHCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCHHH
YAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHCCHHHHHHHH
YTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLTISKLESLPIFVPIYRLSL
HHHHHHHCCCCHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHEEE
VHIPPVWPELRAFARRVSRYHLQTHYFALPVVGNSLYVRH
EECCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]