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The map label for this gene is malE

Identifier: 187731820

GI number: 187731820

Start: 1943662

End: 1944894

Strand: Reverse

Name: malE

Synonym: SbBS512_E2135

Alternate gene names: 187731820

Gene position: 1944894-1943662 (Counterclockwise)

Preceding gene: 187732717

Following gene: 187731503

Centisome position: 42.13

GC content: 40.31

Gene sequence:

>1233_bases
ATGAAGCTTTCAAATATCGTCACAGTTATAATTCTTGCAATTTCCAGTACGCTAACACCCCAGGCGATGGCAGAAAAATT
AATTCCTGAAACTGATGCTGAGTTGTTAGTTTGGTCTGATGCCACAAGCGTGGAATATATGAAATATGCAGCAAAGGAAT
TCAATAAAGATTTTGGCTACAAGGTAAAGTTTACATTTCGCAATATAGCGCCAATGGATGCAGCATCAAGAATTATGCAG
GATGGGGGTACGACTCGTGTAGCTGATGTAGCTGAAATTGAACATGATACCCTGGGGCGGTTAGTCGTTGCTGGCGGGGT
TATGGAAAACATGGTCTCAGCTGAGCGGATTAAAAAAACATTTATTCCAGGCGCAGTATCGGCAGCTACATATAATAACA
TCAGCTATGGTTTTCCTGTAAGTTTCGCAACGCTGGCGCTTTTCTATAATAAGGATTTGTTAAACACCGCACCAAAAACA
TTCGAAGAAATCAATACTTTCAGTGAAAAGTTTAATAATTCATCCGAGCATAAATATGCTCTGCTATGGGATGTACAAAA
TTATTATGTTTCACGTATGTTTATTACCTTGTATGGTGCCAACGAATTCGGTAAAATCGGTAACGATCCTAAAGCTCTAG
GCATCGCTTCATCTGAAGCGAAGAAAGGGCTAGAGACGATGAAACGCTTAAAGAAAGCGAATCCCTCTAATCCTCTTGAT
ATGGGTAATCCACAAGTTCTAAGAGGTCTGTTTAATGAAGGTAAAGTTGCTGCTGTAATCGACGGACCTTGGTCCATACA
AGGTTACATTGACAGCGGAATCAATTTTGGCGTGACACGCATCCCAACATTAGATGGTCATCAGCCTCGCACATTTTCAA
CAGTACGGCTGGCCGTTGTAAGCTCATTTACCGCATATCCTCATGCCGCTGAATTATTCGCTGACTACCTGACAACGGAT
AAGATGTTGATGAAGCGTTATGAAATGACGAATCTCATCCCACCAATCGATTCACTTATGAACAAAATTAGCCAGACAGG
TAGTGAAGCTATAAAAGCAATTATTGCTCAAGCTAATTATTCTGACGCAATGCCATCAATACCGGAAATGTCTTATTTAT
GGTCTCCAATGACTAATGCTATTTTGGCTACCTGGGTTGAGAATAAAACACCGGATGAAGTTTTAAATCATGCACAAACA
ATTATTGAAGAACAACTTTCGCTTCAGGAGTAA

Upstream 100 bases:

>100_bases
TGACAGTTGATTGGCCTTGATTTGTGTTGATAATGGCGTGAATGCTTATGTAGTCGTACATCATATACTACTTAAAGATG
TTATTCATGAGGTAAATATA

Downstream 100 bases:

>100_bases
TTGTGAATACACAACAACTGAAAATGAAGAGTGCACCTGTGCTACCTATTTCCTGTTTGATTATGGGCGGGACTCAGTTA
TCACGTCATTATTATGTGAA

Product: maltose/maltodextrin-binding periplasmic protein MalE

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MKLSNIVTVIILAISSTLTPQAMAEKLIPETDAELLVWSDATSVEYMKYAAKEFNKDFGYKVKFTFRNIAPMDAASRIMQ
DGGTTRVADVAEIEHDTLGRLVVAGGVMENMVSAERIKKTFIPGAVSAATYNNISYGFPVSFATLALFYNKDLLNTAPKT
FEEINTFSEKFNNSSEHKYALLWDVQNYYVSRMFITLYGANEFGKIGNDPKALGIASSEAKKGLETMKRLKKANPSNPLD
MGNPQVLRGLFNEGKVAAVIDGPWSIQGYIDSGINFGVTRIPTLDGHQPRTFSTVRLAVVSSFTAYPHAAELFADYLTTD
KMLMKRYEMTNLIPPIDSLMNKISQTGSEAIKAIIAQANYSDAMPSIPEMSYLWSPMTNAILATWVENKTPDEVLNHAQT
IIEEQLSLQE

Sequences:

>Translated_410_residues
MKLSNIVTVIILAISSTLTPQAMAEKLIPETDAELLVWSDATSVEYMKYAAKEFNKDFGYKVKFTFRNIAPMDAASRIMQ
DGGTTRVADVAEIEHDTLGRLVVAGGVMENMVSAERIKKTFIPGAVSAATYNNISYGFPVSFATLALFYNKDLLNTAPKT
FEEINTFSEKFNNSSEHKYALLWDVQNYYVSRMFITLYGANEFGKIGNDPKALGIASSEAKKGLETMKRLKKANPSNPLD
MGNPQVLRGLFNEGKVAAVIDGPWSIQGYIDSGINFGVTRIPTLDGHQPRTFSTVRLAVVSSFTAYPHAAELFADYLTTD
KMLMKRYEMTNLIPPIDSLMNKISQTGSEAIKAIIAQANYSDAMPSIPEMSYLWSPMTNAILATWVENKTPDEVLNHAQT
IIEEQLSLQE
>Mature_410_residues
MKLSNIVTVIILAISSTLTPQAMAEKLIPETDAELLVWSDATSVEYMKYAAKEFNKDFGYKVKFTFRNIAPMDAASRIMQ
DGGTTRVADVAEIEHDTLGRLVVAGGVMENMVSAERIKKTFIPGAVSAATYNNISYGFPVSFATLALFYNKDLLNTAPKT
FEEINTFSEKFNNSSEHKYALLWDVQNYYVSRMFITLYGANEFGKIGNDPKALGIASSEAKKGLETMKRLKKANPSNPLD
MGNPQVLRGLFNEGKVAAVIDGPWSIQGYIDSGINFGVTRIPTLDGHQPRTFSTVRLAVVSSFTAYPHAAELFADYLTTD
KMLMKRYEMTNLIPPIDSLMNKISQTGSEAIKAIIAQANYSDAMPSIPEMSYLWSPMTNAILATWVENKTPDEVLNHAQT
IIEEQLSLQE

Specific function: Binding protein for cyclodextrin; involved in its cellular uptake. Interacts with all natural cyclodextrins:alpha, beta and gamma [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=403, Percent_Identity=28.287841191067, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 45403; Mature: 45403

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSNIVTVIILAISSTLTPQAMAEKLIPETDAELLVWSDATSVEYMKYAAKEFNKDFGY
CCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCE
KVKFTFRNIAPMDAASRIMQDGGTTRVADVAEIEHDTLGRLVVAGGVMENMVSAERIKKT
EEEEEECCCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIPGAVSAATYNNISYGFPVSFATLALFYNKDLLNTAPKTFEEINTFSEKFNNSSEHKYA
HCCCCCHHHEECCCCCCCCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHCCCCCCEEE
LLWDVQNYYVSRMFITLYGANEFGKIGNDPKALGIASSEAKKGLETMKRLKKANPSNPLD
EEEEHHHHHHHHHEEEEECCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
MGNPQVLRGLFNEGKVAAVIDGPWSIQGYIDSGINFGVTRIPTLDGHQPRTFSTVRLAVV
CCCHHHHHHHHCCCCEEEEECCCEEEEEEECCCCCCCEEECCCCCCCCCCCHHHHHHHHH
SSFTAYPHAAELFADYLTTDKMLMKRYEMTNLIPPIDSLMNKISQTGSEAIKAIIAQANY
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
SDAMPSIPEMSYLWSPMTNAILATWVENKTPDEVLNHAQTIIEEQLSLQE
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLSNIVTVIILAISSTLTPQAMAEKLIPETDAELLVWSDATSVEYMKYAAKEFNKDFGY
CCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCE
KVKFTFRNIAPMDAASRIMQDGGTTRVADVAEIEHDTLGRLVVAGGVMENMVSAERIKKT
EEEEEECCCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIPGAVSAATYNNISYGFPVSFATLALFYNKDLLNTAPKTFEEINTFSEKFNNSSEHKYA
HCCCCCHHHEECCCCCCCCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHCCCCCCEEE
LLWDVQNYYVSRMFITLYGANEFGKIGNDPKALGIASSEAKKGLETMKRLKKANPSNPLD
EEEEHHHHHHHHHEEEEECCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
MGNPQVLRGLFNEGKVAAVIDGPWSIQGYIDSGINFGVTRIPTLDGHQPRTFSTVRLAVV
CCCHHHHHHHHCCCCEEEEECCCEEEEEEECCCCCCCEEECCCCCCCCCCCHHHHHHHHH
SSFTAYPHAAELFADYLTTDKMLMKRYEMTNLIPPIDSLMNKISQTGSEAIKAIIAQANY
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
SDAMPSIPEMSYLWSPMTNAILATWVENKTPDEVLNHAQTIIEEQLSLQE
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]