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The map label for this gene is mak [H]

Identifier: 187731576

GI number: 187731576

Start: 302291

End: 303076

Strand: Direct

Name: mak [H]

Synonym: SbBS512_E0313

Alternate gene names: 187731576

Gene position: 302291-303076 (Clockwise)

Preceding gene: 187734168

Following gene: 187731565

Centisome position: 6.55

GC content: 55.22

Gene sequence:

>786_bases
GTGCGTATAGGTATCGATTTAGGCGGCACCAAAACTGAAGTGATTGCACTGGGCGATGCAGGGGAGCAGTTGTACCGCCA
TCGTCTGCCCACGCCGCGTGATGATTACCGGCAGACTATTGAAACGATCGCCACGTTGGTTGATATGGCGGAGCAGGCGA
CGGGGCAGCGCGGAACGGTAGGTATGGGCATTCCTGGCTCAATTTCGCCTTACACCGGTGTGGTGAAGAATGCCAATTCA
ACCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAA
CTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGAT
GCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCAGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTA
CCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTT
TATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGG
TTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTG
AATATTCTCGATCCGGATGTGATTGTCCTGGGAGGTGCCCCTATTAAGTTGTCAAGCATGTTATGA

Upstream 100 bases:

>100_bases
AAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGTCCGTCTCTACGCTATTGATATTG
AAAAAAATAAGGAGAGTACC

Downstream 100 bases:

>100_bases
CCCCTGCGGGGTATAAAAAGTCCCGTCGCGCATCATGGCGAACAGAACGTCGCAGCGTCGTCTCGCCAGGGCGATAAGCG
CCTGATTGTGTCGTTTTCCC

Product: ROK family protein

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIATLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANS
TWLNGQPFDKDLSARLQREVRLANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHISGNGTAGEWGHNPL
PWMDEDELRYREEVPCYCGKQGCIETFISGTGFAMDYRRLSGHALKGSEIIRLVEESDPVAELALRRYELRLAKSLAHVV
NILDPDVIVLGGAPIKLSSML

Sequences:

>Translated_261_residues
MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIATLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANS
TWLNGQPFDKDLSARLQREVRLANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHISGNGTAGEWGHNPL
PWMDEDELRYREEVPCYCGKQGCIETFISGTGFAMDYRRLSGHALKGSEIIRLVEESDPVAELALRRYELRLAKSLAHVV
NILDPDVIVLGGAPIKLSSML
>Mature_261_residues
MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIATLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANS
TWLNGQPFDKDLSARLQREVRLANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHISGNGTAGEWGHNPL
PWMDEDELRYREEVPCYCGKQGCIETFISGTGFAMDYRRLSGHALKGSEIIRLVEESDPVAELALRRYELRLAKSLAHVV
NILDPDVIVLGGAPIKLSSML

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI87081733, Length=252, Percent_Identity=99.6031746031746, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI1787363, Length=260, Percent_Identity=42.3076923076923, Blast_Score=181, Evalue=5e-47,
Organism=Escherichia coli, GI1786891, Length=264, Percent_Identity=22.7272727272727, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1790522, Length=271, Percent_Identity=26.1992619926199, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 27954; Mature: 27954

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIATLVDMAEQATGQRGTV
CEEEEECCCCCEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCE
GMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRLANDANCLAVSEAVDGAAA
ECCCCCCCCCCHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHCCHHH
GAQTVFAVIIGTGCGAGVAFNGRAHISGNGTAGEWGHNPLPWMDEDELRYREEVPCYCGK
HHHHEEEEEEECCCCCCEEECCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCC
QGCIETFISGTGFAMDYRRLSGHALKGSEIIRLVEESDPVAELALRRYELRLAKSLAHVV
HHHHHHHHCCCCCEEHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
NILDPDVIVLGGAPIKLSSML
HHCCCCEEEECCCCEECCCCC
>Mature Secondary Structure
MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIATLVDMAEQATGQRGTV
CEEEEECCCCCEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCE
GMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRLANDANCLAVSEAVDGAAA
ECCCCCCCCCCHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHCCHHH
GAQTVFAVIIGTGCGAGVAFNGRAHISGNGTAGEWGHNPLPWMDEDELRYREEVPCYCGK
HHHHEEEEEEECCCCCCEEECCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCC
QGCIETFISGTGFAMDYRRLSGHALKGSEIIRLVEESDPVAELALRRYELRLAKSLAHVV
HHHHHHHHCCCCCEEHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
NILDPDVIVLGGAPIKLSSML
HHCCCCEEEECCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]