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The map label for this gene is mak [H]

Identifier: 187731565

GI number: 187731565

Start: 304501

End: 304650

Strand: Direct

Name: mak [H]

Synonym: SbBS512_E0314

Alternate gene names: 187731565

Gene position: 304501-304650 (Clockwise)

Preceding gene: 187731576

Following gene: 187733926

Centisome position: 6.6

GC content: 52.0

Gene sequence:

>150_bases
ATGAGCAATGTAGACCGTTTATATCAAACGGTTCCGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCC
GGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAA

Upstream 100 bases:

>100_bases
GACATCACCCCCCGGATCATGGACGACTGGGGGCAGTAATCATGCCGGGTCTGGCTGGCTAACACCCCATTATAAGGGGG
ACGAATAAAGTAACGGGGGG

Downstream 100 bases:

>100_bases
AAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGC
GTAGGGCGGATAAGGCGTTC

Product: ROK family protein

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 49; Mature: 48

Protein sequence:

>49_residues
MSNVDRLYQTVPQLIKQFVFGGECETPVRKAKHGDSSGVRGAAWLWPQE

Sequences:

>Translated_49_residues
MSNVDRLYQTVPQLIKQFVFGGECETPVRKAKHGDSSGVRGAAWLWPQE
>Mature_48_residues
SNVDRLYQTVPQLIKQFVFGGECETPVRKAKHGDSSGVRGAAWLWPQE

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI87081733, Length=49, Percent_Identity=97.9591836734694, Blast_Score=104, Evalue=1e-24,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 5518; Mature: 5387

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVDRLYQTVPQLIKQFVFGGECETPVRKAKHGDSSGVRGAAWLWPQE
CCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNVDRLYQTVPQLIKQFVFGGECETPVRKAKHGDSSGVRGAAWLWPQE
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]