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The map label for this gene is tnaA

Identifier: 187731211

GI number: 187731211

Start: 3939016

End: 3940431

Strand: Reverse

Name: tnaA

Synonym: SbBS512_E4214

Alternate gene names: 187731211

Gene position: 3940431-3939016 (Counterclockwise)

Preceding gene: 187733846

Following gene: 187733123

Centisome position: 85.36

GC content: 50.49

Gene sequence:

>1416_bases
ATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGAGCCAGTAAAACGTACTACTCGCGCTTATCG
TGAAGAAGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATCGCGAAGATGTGTTTATCGATTTACTGACCGACA
GCGGCACCGGGGCGGTAACCCAAAGTATGCAGGCAGCGATGATGCGCGGCGACGAAGCCTACAGCGGCAGCCGCAGCTAC
TATGCGTTAGCCGAGTCAGTGAAAAATATCTTTGGTTATCAATATACTATTCCGACTCACCAGGGCCGTGGCGCAGAGCA
AATCTATATTCCGGTACTGATTAAAAAACGCGAGCAGGAAAAAGGCCTGGATCGCAGCAAAATGGTGGCATTCTCTAACT
ATTTCTTTGATACCACGCAGGGCCATAGCCAGATTAACGGCTGTACCGTGCGTAACGTCTATATCAAAGAAGCCTTCGAT
ACGGGCGTGCGTTACGACTTTAAAGGCAACTTTGACCTCGAAGGATTAGAACGCGGTATTGAAGAAGTTGGCCCGAATAA
CGTGCCGTATATCGTTGCAACCATCACCAGTAACTCTGCAGGTGGTCAGCCGGTTTCACTGGCAAACTTAAAAGCGATGT
ACAGCATCGCGAAGAAATACGATATTCCGGTGGTAATGGACTCCGCACGCTTTGCTGAAAACGCCTATTTCATCAAGCAG
CGTGAAGCAGAATACAAAGACTGGACCATCGAGCAGATCACCCGCGAAACCTACAAATATGCCGATATGCTGGCGATGTC
CGCCAAGAAAGATGCGATGGTGCCGATGGGCGGCTTGCTGTGCATGAAAGACGACAGCTTCTTTGATGTGTACACCGAGT
GCAGAACCCTTTGCGTGGTGCAGGAAGGCTTCCCGACATATGGCGGCCTGGAAGGCGGCGCGATGGAGCGTCTGGCGGTA
GGTCTGTATGACGGCATGAATCTCGACTGGCTGGCTTATCGTATCGCGCAGGTACAGTATCTGGTCGATGGTCTGGAAGA
GATTGGCGTTGTCTGCCAGCAGGCGGGCGGTCACGCGGCATTCGTTGATGCCGGTAAACTGCTGCCGCATATCCCGGCAG
ACCAGTTCCCGGCACAGGCGCTGGCGTGCGAGCTGTATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTG
TTAGGCCGCGATCCGAAAACCGGTAAACAACTGCCATGCCCGGCTGAACTGCTGCGTTTAACCATTCCGCGCGCAACATA
TACTCAAACACATATGGACTTCATTATTGAAGCCTTTAAACATGTGAAAGAGAACGCGGCGAATATTAAAGGATTAACCT
TTACCTACGAACCAAAAGTATTGCGTCACTTCACCGCAAAACTGAAAGAAGTTTAA

Upstream 100 bases:

>100_bases
TAAATGATGGTGCTTGTATATATACATGGCGAATTAATGGATATTGCAGATGTAATATTCACAGGGATCACTGTAATTAA
AATAAATGAAGGATTATGTA

Downstream 100 bases:

>100_bases
TTAATACTACAGAGTGGCTATAAGGATGTTAGCCACTCTCTTACCCTACATCCTCAATAACAAAAATAGCCTTCCTCTAA
AGGTGGCATCATGACTGATC

Product: tryptophanase

Products: NA

Alternate protein names: L-tryptophan indole-lyase; TNase [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDREDVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSY
YALAESVKNIFGYQYTIPTHQGRGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAKKYDIPVVMDSARFAENAYFIKQ
REAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPMGGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAV
GLYDGMNLDWLAYRIAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIRAVEIGSFL
LGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEPKVLRHFTAKLKEV

Sequences:

>Translated_471_residues
MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDREDVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSY
YALAESVKNIFGYQYTIPTHQGRGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAKKYDIPVVMDSARFAENAYFIKQ
REAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPMGGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAV
GLYDGMNLDWLAYRIAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIRAVEIGSFL
LGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEPKVLRHFTAKLKEV
>Mature_471_residues
MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDREDVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSY
YALAESVKNIFGYQYTIPTHQGRGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAKKYDIPVVMDSARFAENAYFIKQ
REAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPMGGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAV
GLYDGMNLDWLAYRIAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIRAVEIGSFL
LGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEPKVLRHFTAKLKEV

Specific function: Tryptophan catabolism. [C]

COG id: COG3033

COG function: function code E; Tryptophanase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-eliminating lyase family [H]

Homologues:

Organism=Escherichia coli, GI87082323, Length=471, Percent_Identity=99.7876857749469, Blast_Score=979, Evalue=0.0,

Paralogues:

None

Copy number: 7160 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 460 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR011166
- InterPro:   IPR013440
- InterPro:   IPR018176 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: =4.1.99.1 [H]

Molecular weight: Translated: 52843; Mature: 52843

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00853 BETA_ELIM_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDREDVFIDLLTDSGTGAVT
CCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEECCCCCHHH
QSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQGRGAEQIYIPVLIKKREQE
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEEEEEECCHHH
KGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFDTGVRYDFKGNFDLEGLERGI
CCCCHHHEEEEHHCEEECCCCCCCCCCEEEEEEEEEHHHCCCCEEEECCCCCHHHHHHHH
EEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAKKYDIPVVMDSARFAENAYFIKQ
HHHCCCCCCEEEEEEECCCCCCCCEEHHHHHHHHHHHHHCCCCEEECCHHHHCCEEEEEC
REAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPMGGLLCMKDDSFFDVYTECRTLCVV
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHEEEE
QEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYRIAQVQYLVDGLEEIGVVCQQAGGHAA
ECCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
FVDAGKLLPHIPADQFPAQALACELYKVAGIRAVEIGSFLLGRDPKTGKQLPCPAELLRL
EEECCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCHHHCCCCCCCCCCCCCHHHHHHE
TIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEPKVLRHFTAKLKEV
ECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHCC
>Mature Secondary Structure
MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDREDVFIDLLTDSGTGAVT
CCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEECCCCCHHH
QSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQGRGAEQIYIPVLIKKREQE
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEEEEEECCHHH
KGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFDTGVRYDFKGNFDLEGLERGI
CCCCHHHEEEEHHCEEECCCCCCCCCCEEEEEEEEEHHHCCCCEEEECCCCCHHHHHHHH
EEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAKKYDIPVVMDSARFAENAYFIKQ
HHHCCCCCCEEEEEEECCCCCCCCEEHHHHHHHHHHHHHCCCCEEECCHHHHCCEEEEEC
REAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPMGGLLCMKDDSFFDVYTECRTLCVV
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHEEEE
QEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYRIAQVQYLVDGLEEIGVVCQQAGGHAA
ECCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
FVDAGKLLPHIPADQFPAQALACELYKVAGIRAVEIGSFLLGRDPKTGKQLPCPAELLRL
EEECCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCHHHCCCCCCCCCCCCCHHHHHHE
TIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEPKVLRHFTAKLKEV
ECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA